LOCUS       RC215217                7449 bp    DNA     circular  SYN 
DEFINITION  VILL (Myc-DDK-tagged)-Human villin-like (VILL)
ACCESSION   NM_015873
VERSION     NM_015873.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3606..3697 
                     /label="Myc-DDK"  
     misc_feature    3727..4312 
                     /label="PolyA signal"  
     misc_feature    3784..3803 
                     /label="Restriction Enzyme"  
     misc_feature    4588..5260 
                     /label="ColE1"  
     misc_feature    5690..6484 
                     /label="Kan/Neo"  
     misc_feature    6519..6821 
                     /label="SV40 ori"  
     misc_feature    6907..7384 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3588..3594 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3588 
                     /label="VILL"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACATCAGC AAGGGCCTCC CAGGCATGCA GGGAGGCCTC CACATATGGA 
     1081 TCTCTGAGAA CCGGAAGATG GTGCCGGTAC CCGAGGGGGC TTACGGGAAC TTTTTTGAGG 
     1141 AACACTGCTA TGTCATCCTC CACGTCCCCC AGAGCCCGAA GGCCACGCAG GGGGCGTCCA 
     1201 GCGACCTGCA CTACTGGGTC GGGAAGCAGG CGGGTGCGGA AGCGCAGGGC GCTGCGGAGG 
     1261 CCTTCCAGCA GCGCCTACAG GACGAGCTGG GGGGCCAGAC CGTGCTGCAC CGCGAGGCGC 
     1321 AGGGCCACGA GTCCGACTGC TTCTGCAGCT ACTTCCGCCC GGGAATCATC TACAGGAAGG 
     1381 GAGGCCTAGC ATCTGACCTC AAGCATGTGG AGACCAACTT GTTCAACATC CAGCGACTGC 
     1441 TGCACATCAA AGGGAGGAAG CACGTGTCTG CCACTGAGGT GGAGCTCTCC TGGAACAGCT 
     1501 TTAATAAGGG TGACATCTTC CTGCTGGACC TAGGCAAGAT GATGATTCAG TGGAATGGGC 
     1561 CCAAGACCAG CATTTCTGAG AAGGCTCGGG GGCTGGCTTT GACCTACAGC CTCCGGGACA 
     1621 GGGAACGTGG TGGTGGTCGT GCACAGATTG GTGTGGTGGA TGATGAGGCC AAAGCCCCGG 
     1681 ACCTCATGCA GATCATGGAG GCTGTGCTGG GCCGCAGGGT GGGCAGCCTG CGTGCCGCCA 
     1741 CGCCCAGCAA GGATATCAAC CAGCTGCAGA AGGCCAATGT TCGCCTGTAC CATGTCTATG 
     1801 AGAAGGGCAA AGACCTGGTG GTCCTGGAGT TGGCGACCCC CCCACTGACC CAGGACCTGC 
     1861 TGCAGGAGGA GGACTTCTAC ATCCTGGACC AGGGTGGCTT CAAGATCTAT GTGTGGCAGG 
     1921 GACGCATGTC TAGCCTCCAG GAGAGAAAGG CTGCCTTCAG CCGGGCTGTG GGCTTCATCC 
     1981 AGGCCAAGGG CTACCCGACC TACACCAACG TGGAGGTGGT GAACGACGGC GCCGAGTCGG 
     2041 CCGCGTTCAA GCAGCTCTTC CGGACTTGGT CTGAGAAGCG GCGCAGGAAC CAGAAGCTCG 
     2101 GCGGGAGGGA TAAATCGATT CATGTAAAGC TGGACGTGGG CAAGCTGCAC ACCCAGCCTA 
     2161 AGTTAGCGGC CCAGCTCAGG ATGGTGGACG ACGGCTCTGG GAAGGTGGAG GTGTGGTGCA 
     2221 TCCAGGACTT ACACAGGCAG CCCGTGGACC CCAAGCGTCA TGGACAGCTG TGTGCAGGCA 
     2281 ACTGCTACCT TGTGCTCTAC ACATACCAGA GGCTGGGCCG TGTCCAGTAC ATCCTGTACC 
     2341 TATGGCAGGG CCACCAGGCC ACTGCGGATG AGATTGAGGC CCTGAACAGC AACGCTGAGG 
     2401 AACTAGATGT CATGTATGGT GGCGTCCTAG TACAGGAGCA TGTGACCATG GGCAGCGAGC 
     2461 CCCCCCACTT CCTCGCCATC TTCCAGGGCC AGCTGGTGAT CTTCCAGGAG AGAGCTGGGC 
     2521 ACCATGGAAA GGGGCAGTCA GCATCCACCA CAAGGCTTTT CCAAGTGCAA GGCACTGACA 
     2581 GCCACAACAC CAGGACCATG GAGGTGCCAG CCCGTGCCTC ATCCCTCAAC TCCAGTGACA 
     2641 TCTTCTTGCT GGTCACAGCC AGCGTCTGCT ACCTCTGGTT TGGGAAGGGC TGTAATGGTG 
     2701 ATCAGCGTGA GATGGCACGG GTGGTGGTCA CTGTCATTTC CAGGAAGAAT GAGGAAACGG 
     2761 TGCTGGAGGG TCAGGAGCCT CCCCACTTCT GGGAGGCCCT GGGAGGCCGG GCCCCCTACC 
     2821 CCAGCAACAA GAGGCTCCCT GAGGAGGTCC CCAGCTTCCA GCCACGACTG TTTGAGTGCT 
     2881 CCAGCCACAT GGGCTGCCTG GTCCTCGCAG AAGTGGGGTT CTTCAGCCAG GAGGACCTGG 
     2941 ACAAGTATGA CATCATGTTA CTGGACACCT GGCAGGAGAT CTTCCTGTGG CTTGGGGAAG 
     3001 CTGCAAGTGA GTGGAAGGAG GCGGTGGCCT GGGGCCAGGA GTACCTGAAG ACTCACCCAG 
     3061 CAGGGAGGAG CCCGGCCACA CCCATCGTGC TGGTCAAGCA GGGCCATGAG CCTCCCACCT 
     3121 TCATTGGATG GTTCTTCACT TGGGACCCCT ACAAGTGGAC TAGCCACCCG TCCCACAAGG 
     3181 AAGTGGTGGA TGGCAGCCCG GCAGCAGCAT CAACCATCTC TGAGATAACA GCAGAAGTCA 
     3241 ACAACTTGCG GCTATCCAGA TGGCCGGGCA ATGGCAGGGC AGGTGCCGTG GCCCTGCAGG 
     3301 CCCTCAAGGG CTCCCAGGAC AGCTCAGAGA ATGATCTGGT GCGAAGCCCC AAGTCGGCTG 
     3361 GCAGCAGAAC CAGCAGCTCC GTCAGCAGCA CCAGCGCCAC GATCAACGGG GGCCTGCGCC 
     3421 GGGAACAACT GATGCACCAG GCTGTTGAGG ACCTGCCAGA GGGCGTGGAC CCTGCCCGCA 
     3481 GGGAGTTCTA TCTCTCAGAC TCTGACTTCC AAGATATCTT TGGGAAATCC AAGGAGGAAT 
     3541 TCTACAGCAT GGCCACGTGG AGGCAGCGGC AGGAGAAAAA GCAGCTGGGC TTCTTCACGC 
     3601 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     3661 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     3721 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     3781 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     3841 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     3901 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     3961 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4021 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4081 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4141 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4201 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4261 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4321 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4381 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4441 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4501 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4561 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     4621 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     4681 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     4741 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     4801 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     4861 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     4921 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     4981 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5041 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5101 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5161 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5221 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5281 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5341 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5401 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     5461 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     5521 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     5581 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     5641 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     5701 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     5761 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     5821 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     5881 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     5941 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6001 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6061 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6121 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6181 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6241 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6301 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6361 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6421 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6481 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6541 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     6601 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     6661 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     6721 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     6781 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6841 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     6901 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     6961 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7021 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7081 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7141 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7201 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7261 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7321 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7381 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7441 GCGAATTTT
//