LOCUS       RC215171                6951 bp    DNA     circular  SYN 
DEFINITION  CAPN9 (Myc-DDK-tagged)-Human calpain 9 (CAPN9), transcript variant 1
ACCESSION   NM_006615
VERSION     NM_006615.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3108..3199 
                     /label="Myc-DDK"  
     misc_feature    3229..3814 
                     /label="PolyA signal"  
     misc_feature    3286..3305 
                     /label="Restriction Enzyme"  
     misc_feature    4090..4762 
                     /label="ColE1"  
     misc_feature    5192..5986 
                     /label="Kan/Neo"  
     misc_feature    6021..6323 
                     /label="SV40 ori"  
     misc_feature    6409..6886 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3090..3096 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3090 
                     /label="CAPN9"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTTACCTC TACCGGGCCC CAGGGCCTCA GGCACACCCG GTTCCCAAGG 
     1081 ACGCCCGGAT CACCCACTCC TCAGGCCAGA GCTTTGAGCA AATGAGGCAG GAGTGCCTGC 
     1141 AGAGAGGCAC CCTGTTTGAG GATGCAGACT TCCCAGCCAG CAATTCCTCC CTGTTCTACA 
     1201 GTGAGAGGCC GCAGATCCCC TTTGTGTGGA AACGACCAGG GGAAATCGTG AAAAACCCAG 
     1261 AATTCATTCT TGGAGGGGCC ACCAGGACTG ATATCTGCCA GGGAGAGCTG GGAGACTGCT 
     1321 GGCTATTAGC CGCCATCGCC TCCCTTACGC TTAATCAAAA AGCACTGGCC AGAGTCATCC 
     1381 CCCAGGACCA AAGATTTGGC CCTGGTTATG CCGGGATATT CCATTTCCAG TTCTGGCAGC 
     1441 ACAGTGAGTG GCTGGACGTG GTGATCGATG ACCGCCTGCC CACCTTCAGG GACCGCTTGG 
     1501 TTTTCCTCCA CTCTGCCGAC CACAACGAGT TCTGGAGCGC CTTGCTGGAA AAAGCCTACG 
     1561 CCAAGCTAAA TGGGAGCTAT GAAGCTCTGA AGGGAGGCAG CGCCATCGAG GCCATGGAAG 
     1621 ACTTCACTGG GGGTGTGGCA GAGACCTTCC AAACTAAAGA GGCCCCCGAG AACTTCTATG 
     1681 AGATTCTAGA GAAGGCTTTG AAGAGAGGCT CCCTGCTGGG CTGCTTCATT GATACCAGAA 
     1741 GTGCTGCAGA ATCTGAGGCC CGGACGCCGT TTGGTCTTAT TAAGGGTCAT GCCTACAGTG 
     1801 TAACGGGAAT TGACCAGGTA AGCTTCCGAG GCCAGAGAAT CGAGCTCATC CGAATCCGGA 
     1861 ACCCTTGGGG CCAGGTTGAG TGGAACGGGT CGTGGAGCGA CAGTTCTCCG GAGTGGCGTT 
     1921 CTGTTGGTCC AGCTGAGCAG AAGCGTCTGT GTCACACTGC TCTGGATGAT GGGGAATTCT 
     1981 GGATGGCATT TCAGGACTTC AAGGCCCACT TTGATAAAGT GGAGATCTGC AACCTCACTC 
     2041 CCGATGCCCT GGAGGAAGAC GCGATCCACA AATGGGAGGT GACGGTCCAT CAGGGAAGCT 
     2101 GGGTTCGCGG CTCCACGGCT GGGGGCTGCC GCAATTTCCT GGATACCTTT TGGACCAATC 
     2161 CACAAATAAA ATTGTCTCTG ACTGAGAAAG ATGAGGGGCA GGAGGAGTGT AGTTTCCTTG 
     2221 TAGCCCTGAT GCAGAAAGAT AGAAGGAAAC TCAAGAGATT TGGTGCCAAT GTGCTGACAA 
     2281 TCGGCTATGC CATTTATGAG TGCCCTGACA AAGACGAACA CCTGAACAAA GACTTCTTCA 
     2341 GATACCACGC TTCTCGGGCC AGAAGCAAGA CGTTCATCAA CCTGAGAGAA GTCTCCGACC 
     2401 GGTTCAAGCT GCCCCCTGGG GAGTACATCC TGATTCCCAG CACTTTTGAG CCCCACCAGG 
     2461 AAGCTGATTT CTGTCTGAGA ATCTTTTCAG AGAAAAAAGC CATTACCCGG GATATGGATG 
     2521 GAAATGTAGA CATTGACCTT CCTGAGCCTC CAAAGCCAAC TCCACCTGAC CAGGAGACAG 
     2581 AGGAGGAGCA GCGGTTTCGG GCTCTGTTTG AACAAGTCGC TGGTGAGGAC ATGGAGGTGA 
     2641 CAGCAGAGGA ACTTGAGTAT GTTTTAAATG CTGTGCTGCA AAAGAAAAAG GACATCAAAT 
     2701 TCAAGAAGCT AAGCCTGATC TCCTGTAAAA ACATCATTTC CCTGATGGAC ACCAGCGGCA 
     2761 ATGGGAAGCT GGAGTTTGAT GAATTCAAAG TGTTCTGGGA CAAGCTGAAG CAGTGGATTA 
     2821 ACCTTTTCCT TCGGTTTGAT GCTGACAAGT CCGGCACCAT GTCTACCTAT GAACTACGGA 
     2881 CTGCACTGAA AGCTGCAGGC TTTCAGCTGA GCAGCCACCT CCTGCAGCTG ATTGTGCTCA 
     2941 GGTATGCGGA TGAGGAGCTC CAGCTGGACT TCGATGACTT CCTCAACTGC CTGGTCCGGC 
     3001 TGGAGAATGC GAGCCGGGTG TTCCAGGCTC TCAGTACAAA GAACAAGGAG TTCATTCATC 
     3061 TCAATATAAA TGAGTTCATC CATTTGACAA TGAACATCAC GCGTACGCGG CCGCTCGAGC 
     3121 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     3181 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     3241 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     3301 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     3361 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     3421 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     3481 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     3541 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     3601 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     3661 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     3721 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     3781 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     3841 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     3901 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     3961 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4021 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4081 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     4141 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     4201 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     4261 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     4321 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     4381 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     4441 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     4501 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     4561 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     4621 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     4681 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     4741 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     4801 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     4861 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     4921 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     4981 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5041 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     5101 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     5161 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     5221 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     5281 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     5341 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     5401 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     5461 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     5521 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     5581 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     5641 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     5701 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     5761 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     5821 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     5881 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     5941 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6001 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6061 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     6121 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     6181 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6241 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     6301 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     6361 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     6421 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     6481 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     6541 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     6601 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     6661 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     6721 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     6781 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     6841 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     6901 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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