LOCUS       RC215163                8841 bp    DNA     circular  SYN 
DEFINITION  MAP4K4 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 2
ACCESSION   NM_145686
VERSION     NM_145686.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4998..5089 
                     /label="Myc-DDK"  
     misc_feature    5119..5704 
                     /label="PolyA signal"  
     misc_feature    5176..5195 
                     /label="Restriction Enzyme"  
     misc_feature    5980..6652 
                     /label="ColE1"  
     misc_feature    7082..7876 
                     /label="Kan/Neo"  
     misc_feature    7911..8213 
                     /label="SV40 ori"  
     misc_feature    8299..8776 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4980..4986 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4980 
                     /label="MAP4K4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAACGAC TCCCCTGCAA AAAGTCTGGT GGACATCGAC CTCTCCTCCC 
     1081 TGCGGGATCC TGCTGGGATT TTTGAGCTGG TGGAAGTGGT TGGAAATGGC ACCTATGGAC 
     1141 AAGTCTATAA GGGTCGACAT GTTAAAACGG GTCAGTTGGC AGCCATCAAA GTTATGGATG 
     1201 TCACTGAGGA TGAAGAGGAA GAAATCAAAC TGGAGATAAA TATGCTAAAG AAATACTCTC 
     1261 ATCACAGAAA CATTGCAACA TATTATGGTG CTTTCATCAA AAAGAGCCCT CCAGGACATG 
     1321 ATGACCAACT CTGGCTTGTT ATGGAGTTCT GTGGGGCTGG GTCCATTACA GACCTTGTGA 
     1381 AGAACACCAA AGGGAACACA CTCAAAGAAG ACTGGATCGC TTACATCTCC AGAGAAATCC 
     1441 TGAGGGGACT GGCACATCTT CACATTCATC ATGTGATTCA CCGGGATATC AAGGGCCAGA 
     1501 ATGTGTTGCT GACTGAGAAT GCAGAGGTGA AACTTGTTGA CTTTGGTGTG AGTGCTCAGC 
     1561 TGGACAGGAC TGTGGGGCGG AGAAATACGT TCATAGGCAC TCCCTACTGG ATGGCTCCTG 
     1621 AGGTCATCGC CTGTGATGAG AACCCAGATG CCACCTATGA TTACAGAAGT GATCTTTGGT 
     1681 CTTGTGGCAT TACAGCCATT GAGATGGCAG AAGGTGCTCC CCCTCTCTGT GACATGCATC 
     1741 CAATGAGAGC ACTGTTTCTC ATTCCCAGAA ACCCTCCTCC CCGGCTGAAG TCAAAAAAAT 
     1801 GGTCGAAGAA GTTTTTTAGT TTTATAGAAG GGTGCCTGGT GAAGAATTAC ATGCAGCGGC 
     1861 CCTCTACAGA GCAGCTTTTG AAACATCCTT TTATAAGGGA TCAGCCAAAT GAAAGGCAAG 
     1921 TTAGAATCCA GCTTAAGGAT CATATAGATC GTACCAGGAA GAAGAGAGGC GAGAAAGATG 
     1981 AAACTGAGTA TGAGTACAGT GGGAGTGAGG AAGAAGAGGA GGAAGTGCCT GAACAGGAAG 
     2041 GAGAGCCAAG TTCCATTGTG AACGTGCCTG GTGAGTCTAC TCTTCGCCGA GATTTCCTGA 
     2101 GACTGCAGCA GGAGAACAAG GAACGTTCCG AGGCTCTTCG GAGACAACAG TTACTACAGG 
     2161 AGCAACAGCT CCGGGAGCAG GAAGAATATA AAAGGCAACT GCTGGCAGAG AGACAGAAGC 
     2221 GGATTGAGCA GCAGAAAGAA CAGAGGCGAC GGCTAGAAGA GCAACAAAGG AGAGAGCGGG 
     2281 AAGCTAGAAG GCAGCAGGAA CGTGAACAGC GAAGGAGAGA ACAAGAAGAA AAGAGGCGTC 
     2341 TAGAGGAGTT GGAGAGAAGG CGCAAAGAAG AAGAGGAGAG GAGACGGGCA GAAGAAGAAA 
     2401 AGAGGAGAGT TGAAAGAGAA CAGGAGTATA TCAGGCGACA GCTAGAAGAG GAGCAGCGGC 
     2461 ACTTGGAAGT CCTTCAGCAG CAGCTGCTCC AGGAGCAGGC CATGTTACTG GAGTGCCGAT 
     2521 GGCGGGAGAT GGAGGAGCAC CGGCAGGCAG AGAGGCTCCA GAGGCAGTTG CAACAAGAAC 
     2581 AAGCATATCT CCTGTCTCTA CAGCATGACC ATAGGAGGCC GCACCCGCAG CACTCGCAGC 
     2641 AGCCGCCACC ACCGCAGCAG GAAAGGAGCA AGCCAAGCTT CCATGCTCCC GAGCCCAAAG 
     2701 CCCACTACGA GCCTGCTGAC CGAGCGCGAG AGGTGGAAGA TAGATTTAGG AAAACTAACC 
     2761 ACAGCTCCCC TGAAGCCCAG TCTAAGCAGA CAGGCAGAGT ATTGGAGCCA CCAGTGCCTT 
     2821 CCCGATCAGA GTCTTTTTCC AATGGCAACT CCGAGTCTGT GCATCCCGCC CTGCAGAGAC 
     2881 CAGCGGAGCC ACAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT GTTTCCCCTG 
     2941 TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC CATCTTCGTT 
     3001 CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT GACTCTAAGT 
     3061 CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC GAGGTGCCTC 
     3121 CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA GATTCCCCAC 
     3181 TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC AGCAGTATTG 
     3241 AGCCCAGGCT TCTGTGGGAG AGAGTGGAGA AGCTGGTGCC CAGACCTGGC AGTGGCAGCT 
     3301 CCTCAGGGTC CAGCAACTCA GGATCCCAGC CCGGGTCTCA CCCTGGGTCT CAGAGTGGCT 
     3361 CCGGGGAACG CTTCAGAGTG AGATCATCAT CCAAGTCTGA AGGCTCTCCA TCTCAGCGCC 
     3421 TGGAAAATGC AGTGAAAAAA CCTGAAGATA AAAAGGAAGT TTTCAGACCC CTCAAGCCTG 
     3481 CTGGCGAAGT GGATCTGACC GCACTGGCCA AAGAGCTTCG AGCAGTGGAA GATGTACGGC 
     3541 CACCTCACAA AGTAACGGAC TACTCCTCAT CCAGTGAGGA GTCGGGGACG ACGGATGAGG 
     3601 AGGACGACGA TGTGGAGCAG GAAGGGGCTG ACGAGTCCAC CTCAGGACCA GAGGACACCA 
     3661 GAGCAGCGTC ATCTCTGAAT TTGAGCAATG GTGAAACGGA ATCTGTGAAA ACCATGATTG 
     3721 TCCATGATGA TGTAGAAAGT GAGCCGGCCA TGACCCCATC CAAGGAGGGC ACTCTAATCG 
     3781 TCCGCCAGAC TCAGTCCGCT AGTAGCACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA 
     3841 CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGCGGAACA ACAGTGACAT 
     3901 CTGTGGTGGG ATTTTCCTGT GATGGGATGA GACCAGAAGC CATAAGGCAA GATCCTACCC 
     3961 GGAAAGGCTC AGTGGTCAAT GTGAATCCTA CCAACACTAG GCCACAGAGT GACACCCCGG 
     4021 AGATTCGTAA ATACAAGAAG AGGTTTAACT CTGAGATTCT GTGTGCTGCC TTATGGGGAG 
     4081 TGAATTTGCT AGTGGGTACA GAGAGTGGCC TGATGCTGCT GGACAGAAGT GGCCAAGGGA 
     4141 AGGTCTATCC TCTTATCAAC CGAAGACGAT TTCAACAAAT GGACGTACTT GAGGGCTTGA 
     4201 ATGTCTTGGT GACAATATCT GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTGTCCTGGT 
     4261 TAAGAAATAA AATACTTCAC AATGATCCAG AAGTTGAGAA GAAGCAGGGA TGGACAACCG 
     4321 TAGGGGATTT GGAAGGATGT GTACATTATA AAGTTGTAAA ATATGAAAGA ATCAAATTTC 
     4381 TGGTGATTGC TTTGAAGAGT TCTGTGGAAG TCTATGCGTG GGCACCAAAG CCATATCACA 
     4441 AATTTATGGC CTTTAAGTCA TTTGGAGAAT TGGTACATAA GCCATTACTG GTGGATCTCA 
     4501 CTGTTGAGGA AGGCCAGAGG TTGAAAGTGA TCTATGGATC CTGTGCTGGA TTCCATGCTG 
     4561 TTGATGTGGA TTCAGGATCA GTCTATGACA TTTATCTACC AACACATATC CAGTGTAGCA 
     4621 TCAAACCCCA TGCAATCATC ATCCTCCCCA ATACAGATGG AATGGAGCTT CTGGTGTGCT 
     4681 ATGAAGATGA GGGGGTTTAT GTAAACACAT ATGGAAGGAT CACCAAGGAT GTAGTTCTAC 
     4741 AGTGGGGAGA GATGCCTACA TCAGTAGCAT ATATTCGATC CAATCAGACA ATGGGCTGGG 
     4801 GAGAGAAGGC CATAGAGATC CGATCTGTGG AAACTGGTCA CTTGGATGGT GTGTTCATGC 
     4861 ACAAAAGGGC TCAAAGACTA AAATTCTTGT GTGAACGCAA TGACAAGGTG TTCTTTGCCT 
     4921 CTGTTCGGTC TGGTGGCAGC AGTCAGGTTT ATTTCATGAC CTTAGGCAGG ACTTCTCTTC 
     4981 TGAGCTGGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     5041 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5101 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5161 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5221 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5281 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5341 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5401 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5461 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5521 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5581 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5641 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5701 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5761 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5821 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5881 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5941 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     6001 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6061 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6121 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6181 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6241 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6301 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6361 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6421 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6481 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6541 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6601 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6661 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6721 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6781 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6841 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6901 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6961 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     7021 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7081 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7141 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7201 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7261 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7321 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7381 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7441 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7501 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7561 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7621 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7681 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7741 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7801 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7861 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7921 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7981 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     8041 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8101 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8161 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8221 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8281 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8341 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8401 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8461 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8521 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8581 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8641 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8701 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8761 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8821 CAAAAATTTA ACGCGAATTT T
//