LOCUS       RC215117                7917 bp    DNA     circular  SYN 
DEFINITION  NLRP7 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 7 (NLRP7), transcript variant 1
ACCESSION   NM_139176
VERSION     NM_139176.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4072..4163 
                     /label="Myc-DDK"  
     misc_feature    4193..4778 
                     /label="PolyA signal"  
     misc_feature    4250..4269 
                     /label="Restriction Enzyme"  
     misc_feature    5054..5726 
                     /label="ColE1"  
     misc_feature    6156..6950 
                     /label="Kan/Neo"  
     misc_feature    6985..7287 
                     /label="SV40 ori"  
     misc_feature    7373..7850 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4047..4054 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4047 
                     /label="NLRP7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACATCGCCC CAGCTAGAGT GGACTCTGCA GACCCTTCTG GAGCAGCTGA 
     1081 ACGAGGATGA ATTAAAGAGT TTCAAATCCC TTTTATGGGC TTTTCCCCTC GAAGACGTGC 
     1141 TACAGAAGAC CCCATGGTCT GAGGTGGAAG AGGCTGATGG CAAGAAACTG GCAGAAATTC 
     1201 TGGTCAACAC CTCCTCAGAA AATTGGATAA GGAATGCGAC TGTGAACATC TTGGAAGAGA 
     1261 TGAATCTCAC GGAATTGTGT AAGATGGCAA AGGCTGAGAT GATGGAGGAC GGACAGGTGC 
     1321 AAGAAATAGA TAATCCTGAG CTGGGAGATG CAGAAGAAGA CTCGGAGTTA GCAAAGCCAG 
     1381 GTGAAAAGGA AGGATGGAGA AATTCAATGG AGAAACAGTC TTTGGTCTGG AAGAACACCT 
     1441 TTTGGCAAGG AGACATTGAC AATTTCCATG ACGACGTCAC TCTGAGAAAC CAACGGTTCA 
     1501 TTCCATTCTT GAATCCCAGA ACACCCAGGA AGCTAACACC TTACACGGTG GTGCTGCACG 
     1561 GCCCCGCAGG CGTGGGGAAA ACCACGCTGG CCAAAAAGTG TATGCTGGAC TGGACAGACT 
     1621 GCAACCTCAG CCCGACGCTC AGATACGCGT TCTACCTCAG CTGCAAGGAG CTCAGCCGCA 
     1681 TGGGCCCCTG CAGTTTTGCA GAGCTGATCT CCAAAGACTG GCCTGAATTG CAGGATGACA 
     1741 TTCCAAGCAT CCTAGCCCAA GCACAGAGAA TCCTGTTCGT GGTCGATGGC CTTGATGAGC 
     1801 TGAAAGTCCC ACCTGGGGCG CTGATCCAGG ACATCTGCGG GGACTGGGAG AAGAAGAAGC 
     1861 CGGTGCCCGT CCTCCTGGGG AGTTTGCTGA AGAGGAAGAT GTTACCCAGG GCAGCCTTGC 
     1921 TGGTCACCAC GCGGCCCAGG GCACTGAGGG ACCTCCAGCT CCTGGCGCAG CAGCCGATCT 
     1981 ACGTAAGGGT GGAGGGCTTC CTGGAGGAGG ACAGGAGGGC CTATTTCCTG AGACACTTTG 
     2041 GAGACGAGGA CCAAGCCATG CGTGCCTTTG AGCTAATGAG GAGCAACGCG GCCCTGTTCC 
     2101 AGCTGGGCTC GGCCCCCGCG GTGTGCTGGA TTGTGTGCAC GACTCTGAAG CTGCAGATGG 
     2161 AGAAGGGGGA GGACCCGGTC CCCACCTGCC TCACCCGCAC GGGGCTGTTC CTGCGTTTCC 
     2221 TCTGCAGCCG GTTCCCGCAG GGCGCACAGC TGCGGGGCGC GCTGCGGACG CTGAGCCTCC 
     2281 TGGCCGCGCA GGGCCTGTGG GCGCAGATGT CCGTGTTCCA CCGAGAGGAC CTGGAAAGGC 
     2341 TCGGGGTGCA GGAGTCCGAC CTCCGTCTGT TCCTGGACGG AGACATCCTC CGCCAGGACA 
     2401 GAGTCTCCAA AGGCTGCTAC TCCTTCATCC ACCTCAGCTT CCAGCAGTTT CTCACTGCCC 
     2461 TGTTCTACGC CCTGGAGAAG GAGGAGGGGG AGGACAGGGA CGGCCACGCC TGGGACATCG 
     2521 GGGACGTACA GAAGCTGCTT TCCGGAGAAG AAAGACTCAA GAACCCCGAC CTGATTCAAG 
     2581 TAGGACACTT CTTATTCGGC CTCGCTAACG AGAAGAGAGC CAAGGAGTTG GAGGCCACTT 
     2641 TTGGCTGCCG GATGTCACCG GACATCAAAC AGGAATTGCT GCAATGCAAA GCACATCTTC 
     2701 ATGCAAATAA GCCCTTATCC GTGACCGACC TGAAGGAGGT CTTGGGCTGC CTGTATGAGT 
     2761 CTCAGGAGGA GGAGCTGGCG AAGGTGGTGG TGGCCCCGTT CAAGGAAATT TCTATTCACC 
     2821 TGACAAATAC TTCTGAAGTG ATGCATTGTT CCTTCAGCCT GAAGCATTGT CAAGACTTGC 
     2881 AGAAACTCTC ACTGCAGGTA GCAAAGGGGG TGTTCCTGGA GAATTACATG GATTTTGAAC 
     2941 TGGACATTGA ATTTGAAAGC TCAAACAGCA ACCTCAAGTT TCTGGAAGTG AAACAAAGCT 
     3001 TCCTGAGTGA CTCTTCTGTG CGGATTCTTT GTGACCACGT AACCCGTAGC ACCTGTCATC 
     3061 TGCAGAAAGT GGAGATTAAA AACGTCACCC CTGACACCGC GTACCGGGAC TTCTGTCTTG 
     3121 CTTTCATTGG GAAGAAGACC CTCACGCACC TGACCCTGGC AGGGCACATC GAGTGGGAAC 
     3181 GCACGATGAT GCTGATGCTG TGTGACCTGC TCAGAAATCA TAAATGCAAC CTGCAGTACC 
     3241 TGAGGTTGGG AGGTCACTGT GCCACCCCGG AGCAGTGGGC TGAATTCTTC TATGTCCTCA 
     3301 AAGCCAACCA GTCCCTGAAG CACCTGCGTC TCTCAGCCAA TGTGCTCCTG GATGAGGGTG 
     3361 CCATGTTGCT GTACAAGACC ATGACACGCC CAAAACACTT CCTGCAGATG TTGTCGTTGG 
     3421 AAAACTGTCG TCTTACAGAA GCCAGTTGCA AGGACCTTGC TGCTGTCTTG GTTGTCAGCA 
     3481 AGAAGCTGAC ACACCTGTGC TTGGCCAAGA ACCCCATTGG GGATACAGGG GTGAAGTTTC 
     3541 TGTGTGAGGG CTTGAGTTAC CCTGATTGTA AACTGCAGAC CTTGGTGTTA CAGCAATGCA 
     3601 GCATAACCAA GCTTGGCTGT AGATATCTCT CAGAGGCGCT CCAAGAAGCC TGCAGCCTCA 
     3661 CAAACCTGGA CTTGAGTATC AACCAGATAG CTCGTGGATT GTGGATTCTC TGTCAGGCAT 
     3721 TAGAGAATCC AAACTGTAAC CTAAAACACC TACGCCTCTG GAGCTGCTCC CTCATGCCTT 
     3781 TCTATTGTCA GCATCTTGGA TCTGCTCTCC TCAGCAATCA GAAGCTTGAA ACTCTGGACC 
     3841 TGGGCCAGAA TCATTTGTGG AAGAGTGGCA TAATTAAGCT CTTTGGGGTT CTAAGACAAA 
     3901 GAACTGGATC CTTGAAGATA CTCAGGTTGA AGACCTATGA AACTAATTTG GAAATCAAGA 
     3961 AGCTGTTGGA GGAAGTGAAA GAAAAGAATC CCAAGCTGAC TATTGATTGC AATGCTTCCG 
     4021 GGGCAACGGC ACCTCCGTGC TGTGACTTTT TTTGCAGCGG ACCGACGCGT ACGCGGCCGC 
     4081 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4141 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4201 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4261 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4321 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4381 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4441 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4501 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4561 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4621 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4681 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4741 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4801 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4861 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4921 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4981 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5041 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5101 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5161 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5221 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5281 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5341 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5401 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5461 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5521 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5581 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5641 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5701 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5761 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5821 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5881 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5941 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6001 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6061 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6121 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6181 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6241 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6301 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6361 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6421 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6481 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6541 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6601 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6661 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6721 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6781 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6841 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6901 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6961 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7021 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7081 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7141 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7201 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7261 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7321 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7381 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7441 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7501 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7561 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7621 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7681 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7741 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7801 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7861 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//