LOCUS       RC215090L3                9272 bp    DNA     circular  SYN 
DEFINITION  MAGED4 (Myc-DDK-tagged)-Human melanoma antigen family D, 4 (MAGED4)
ACCESSION   NM_001098800
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7563..7743 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8471..9130 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7791..8410 
                     /label="ColE1"  
     misc_feature    5603..5626 
                     /label="DDK"  
     misc_feature    6438..6458 
                     /label="LR50"  
     misc_feature    5555..5626 
                     /label="MYC"  
     misc_feature    5636..5701 
                     /label="P2A"  
     misc_feature    5702..6301 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    6447..7038 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5537..5543 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5537 
                     /label="MAGED4"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCTGAGGGA AGCTTCAGCG TGCAATCGGA 
     3361 AAGCTACAGT GTTGAAGACA TGGATGAGGG TAGCGACGAA GTCGGGGAGG AAGAGATGGT 
     3421 TGAAGGCAAC GACTATGAAG AATTCGGTGC GTTTGGTGGC TATGGCACCC TCACCAGCTT 
     3481 TGACATCCAT ATCCTCAGAG CCTTCGGAAG CTTGGGTCCA GGCCTTCGCA TCTTATCGAA 
     3541 TGAGCCCTGG GAACTGGAAA ACCCTGTGCT GGCCCAGACC CTGGTGGAGG CATTGCAGCT 
     3601 GGATCCGGAA ACACTTGCCA ATGAGACGGC CGCCCGTGCT GCCAACGTAG CCCGCGCCGC 
     3661 CGCCTCCAAC CGTGCGGCTC GGGCCGCTGC CGCCGCTGCC CGTACCGCCT TCAGTCAGGT 
     3721 GGTCGCTAGC CACCGGGTGG CCACGCCGCA GGTCTCAGGA GAGGATACCC AGCCCACGAC 
     3781 CTACGCCGCC GAGGCTCAGG GGCCCACCCC TGAGCCACCC CTTGCTTCTC CGCAGACCTC 
     3841 CCAGATGTTA GTCACCAGTA AGATGGCTGC CCCCGAGGCT CCGGCAACCT CCGCACAGTC 
     3901 CCAGACAGGC TCCCCGGCCC AGGAGGCTGC TACTGAGGGC CCTAGTAGCG CCTGTGCTTT 
     3961 CTCTCAGGCT CCGTGTGCCA GGGAGGTGGA CGCCAACCGG CCCAGCACAG CCTTCCTGGG 
     4021 CCAGAATGAT GTCTTCGATT TCACTCAGCC GGCAGGTGTC AGTGGCATGG CCTTCCCGCG 
     4081 CCCCAAGAGA CCTGCCCCAG CCCAAGAGGC TGCCACAGAG GGCCCCAGTG CTGCCTCTGG 
     4141 TGTGCCCCAG ACGGGACCTG GCAGGGAGGT GGCAGCCACC CGGCCCAAGA CCACCAAGTC 
     4201 GGGGAAGGCG CTGGCCAAGA CTCGGTGGGT GGAGCCTCAG AATGTTGTGG CAGCAGCTGC 
     4261 TGCCAAGGCC AAGATGGCCA CGAGCATCCC TGAGCCGGAG GGTGCAGCTG CTGCCACTGC 
     4321 TCAGCACAGT GCTGAGCCCT GGGCCAGGAT GGGAGGCAAG AGGACCAAGA AGTCCAAGCA 
     4381 CCTGGATGAT GAGTATGAGA GCAGCGAGGA GGAGAGAGAG ACTCCCGCGG TCCCACCCAC 
     4441 CTGGAGAGCA TCACAGCCCT CATTGACGGT GCGGGCTCAG TTGGCCCCTC GGCCCCCGAT 
     4501 GGCCCCGAGG TCCCAGATAC CCTCAAGGCA CGTACTGTGC CTGCCCCCCC GCAACGTGAC 
     4561 CCTTCTGCAG GAGAGGGCAA ATAAGTTGGT GAAATACCTG ATGATTAAGG ACTACAAGAA 
     4621 GATCCCCATC AAGCGCGCAG ACATGCTGAA GGATGTCATC AGAGAATATG ATGAACATTT 
     4681 CCCTGAGATC ATTGAACGAG CAACGTACAC CCTGGAAAAG AAGTTTGGGA TCCACCTGAA 
     4741 GGAGATCGAC AAGGAAGAAC ACCTGTATAT TCTTGTCTGC ACACGGGACT CCTCAGCTCG 
     4801 CCTCCTTGGA AAAACCAAGG ACACTCCCAG GCTGAGTCTC CTCTTGGTGA TTCTGGGCGT 
     4861 CATCTTCATG AATGGCAACC GTGCCAGCGA GGCTGTCCTC TGGGAGGCAC TACGCAAGAT 
     4921 GGGACTGCGC CCTGGGGTGA GGCACCCATT CCTCGGCGAT CTGAGGAAGC TCATCACAGA 
     4981 TGACTTTGTG AAGCAGAAGT ACCTGGAATA CAAGAAGATC CCCAACAGCA ACCCACCTGA 
     5041 GTATGAATTC CTCTGGGGCC TGCGAGCCCG CCATGAGACC AGCAAGATGA GGGTCCTGAG 
     5101 ATTCATCGCC CAGAATCAGA ACCGAGACCC CCGGGAATGG AAGGCTCATT TCTTGGAGGC 
     5161 TGTGGATGAT GCTTTCAAGA CAATGGATGT GGATATGGCC GAGGAACATG CCAGGGCCCA 
     5221 GATGAGGGCC CAGATGAATA TCGGGGATGA AGCGCTGATT GGACGGTGGA GCTGGGATGA 
     5281 CATACAAGTC GAGCTCCTGA CCTGGGATGA GGACGGAGAT TTTGGCGATG CCTGGGCCAG 
     5341 GATCCCCTTT GCTTTCTGGG CCAGATACCA TCAGTACATT CTGAATAGCA ACCGTGCCAA 
     5401 CAGGAGGGCC ACGTGGAGAG CTGGCGTCAG CAGTGGCACC AATGGAGGGG CCAGCACCAG 
     5461 CGTCCTAGAT GGCCCCAGCA CCAGCTCCAC CATCCGGACC AGAAATGCTG CCAGAGCTGG 
     5521 CGCCAGCTTC TTCTCCTGGA TCCAGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC 
     5581 AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTG 
     5641 GGTAGGAAGC GGAGCTACTA ACTTCAGCCT GCTGAAGCAG GCTGGAGACG TGGAGGAGAA 
     5701 CCCTGGACCT ATGACCGAGT ACAAGCCCAC GGTGCGCCTC GCCACCCGCG ACGACGTCCC 
     5761 CAGGGCCGTA CGCACCCTCG CCGCCGCGTT CGCCGACTAC CCCGCCACGC GCCACACCGT 
     5821 CGATCCGGAT CGCCACATCG AGCGGGTCAC CGAGCTGCAA GAACTCTTCC TCACGCGCGT 
     5881 CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC GGACGACGGC GCCGCGGTGG CGGTCTGGAC 
     5941 CACGCCGGAG AGCGTCGAAG CGGGGGCGGT GTTCGCCGAG ATCGGCCCGC GCATGGCCGA 
     6001 GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA ACAGATGGAA GGCCTCCTGG CGCCGCACCG 
     6061 GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC CGTCGGCGTC TCGCCCGACC ACCAGGGCAA 
     6121 GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC 
     6181 CGCCTTCCTG GAGACCTCCG CGCCCCGCAA CCTCCCCTTC TACGAGCGGC TCGGCTTCAC 
     6241 CGTCACCGCC GACGTCGAGG TGCCCGAAGG ACCGCGCACC TGGTGCATGA CCCGCAAGCC 
     6301 CGGTGCCTGA TGTACACCCA AACGGCCGGC CGCGGTCTGT ACAAGTAGGA TTCGTCGAGG 
     6361 GACCTAATAA CTTCGTATAG CATACATTAT ACGAAGTTAT ACATGTTTAA GGGTTCCGGT 
     6421 TCCACTAGGT ACAATTCGAT ATCAAGCTTA TCGATAATCA ACCTCTGGAT TACAAAATTT 
     6481 GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG 
     6541 CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT 
     6601 ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG 
     6661 TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC 
     6721 AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG 
     6781 CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT 
     6841 TGTCGGGGAA ATCATCGTCC TTTCCTTGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC 
     6901 GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG 
     6961 GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA 
     7021 TCTCCCTTTG GGCCGCCTCC CCGCATCGAT ACCGTCGACC TCGATCGAGA CCTAGAAAAA 
     7081 CATGGAGCAA TCACAAGTAG CAATACAGCA GCTACCAATG CTGATTGTGC CTGGCTAGAA 
     7141 GCACAAGAGG AGGAGGAGGT GGGTTTTCCA GTCACACCTC AGGTACCTTT AAGACCAATG 
     7201 ACTTACAAGG CAGCTGTAGA TCTTAGCCAC TTTTTAAAAG AAAAGGGGGG ACTGGAAGGG 
     7261 CTAATTCACT CCCAACGAAG ACAAGATATC CTTGATCTGT GGATCTACCA CACACAAGGC 
     7321 TACTTCCCTG ATTGGCAGAA CTACACACCA GGGCCAGGGA TCAGATATCC ACTGACCTTT 
     7381 GGATGGTGCT ACAAGCTAGT ACCAGTTGAG CAAGAGAAGG TAGAAGAAGC CAATGAAGGA 
     7441 GAGAACACCC GCTTGTTACA CCCTGTGAGC CTGCATGGGA TGGATGACCC GGAGAGAGAA 
     7501 GTATTAGAGT GGAGGTTTGA CAGCCGCCTA GCATTTCATC ACATGGCCCG AGAGCTGCAT 
     7561 CCGGACTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT CTCTGGCTAA 
     7621 CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT 
     7681 GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA GTCAGTGTGG 
     7741 AAAATCTCTA GCAGCATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC 
     7801 GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC 
     7861 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA 
     7921 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT 
     7981 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG 
     8041 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC 
     8101 GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG 
     8161 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC 
     8221 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG 
     8281 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC 
     8341 GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT 
     8401 CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT 
     8461 TAAGGGATTT TGGTCATGAT TACGCCCCGC CCTGCCACTC ATCGCAGTAC TGTTGTAATT 
     8521 CATTAAGCAT TCTGCCGACA TGGAAGCCAT CACAAACGGC ATGATGAACC TGAATCGCCA 
     8581 GCGGCATCAG CACCTTGTCG CCTTGCGTAT AATATTTGCC CATGGTGAAA ACGGGGGCGA 
     8641 AGAAGTTGTC CATATTGGCC ACGTTTAAAT CAAAACTGGT GAAACTCACC CAGGGATTGG 
     8701 CTGAGACGAA AAACATATTC TCAATAAACC CTTTAGGGAA ATAGGCCAGG TTTTCACCGT 
     8761 AACACGCCAC ATCTTGCGAA TATATGTGTA GAAACTGCCG GAAATCGTCG TGGTATTCAC 
     8821 TCCAGAGCGA TGAAAACGTT TCAGTTTGCT CATGGAAAAC GGTGTAACAA GGGTGAACAC 
     8881 TATCCCATAT CACCAGCTCA CCGTCTTTCA TTGCCATACG GAACTCCGGA TGAGCATTCA 
     8941 TCAGGCGGGC AAGAATGTGA ATAAAGGCCG GATAAAACTT GTGCTTATTT TTCTTTACGG 
     9001 TCTTTAAAAA GGCCGTAATA TCCAGCTGAA CGGTCTGGTT ATAGGTACAT TGAGCAACTG 
     9061 ACTGAAATGC CTCAAAATGT TCTTTACGAT GCCATTGGGA TATATCAACG GTGGTATATC 
     9121 CAGTGATTTT TTTCTCCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT 
     9181 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 
     9241 CGCGCACATT TCCCCGAAAA GTGCCACCTG AC
//