LOCUS       RC215033                9012 bp    DNA     circular  SYN 
DEFINITION  PHLDB1 (Myc-DDK-tagged)-Human pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant 1
ACCESSION   NM_015157
VERSION     NM_015157.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5169..5260 
                     /label="Myc-DDK"  
     misc_feature    5290..5875 
                     /label="PolyA signal"  
     misc_feature    5347..5366 
                     /label="Restriction Enzyme"  
     misc_feature    6151..6823 
                     /label="ColE1"  
     misc_feature    7253..8047 
                     /label="Kan/Neo"  
     misc_feature    8082..8384 
                     /label="SV40 ori"  
     misc_feature    8470..8947 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5151..5157 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5151 
                     /label="PHLDB1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACGCTCTC AATAGGAACC AAATAGGCCC TGGATGCCAG ACCCAGACCA 
     1081 TGGTGCAGAA AGGACCCTTG GACCTGATCG AGACAGGCAA AGGGCTGAAA GTGCAAACGG 
     1141 ACAAACCCCA CCTGGTGAGC CTGGGCAGTG GGCGACTCAG CACAGCCATC ACCCTCCTGC 
     1201 CGCTGGAGGA AGGGAGGACG GTGATTGGCT CTGCAGCCAG AGACATCTCA CTACAGGGCC 
     1261 CAGGCCTGGC TCCAGAGCAC TGCTACATCG AGAACCTGCG GGGCACCCTC ACCCTCTACC 
     1321 CCTGTGGCAA TGCCTGCACT ATTGATGGGC TCCCTGTCCG GCAGCCTACC CGGCTCACTC 
     1381 AGGGCTGCAT GTTGTGCCTG GGTCAGTCCA CCTTCCTTCG CTTTAACCAC CCGGCTGAAG 
     1441 CCAAGTGGAT GAAAAGCATG ATTCCAGCAG GGGGCCGAGC CCCTGGGCCC CCCTACAGCC 
     1501 CTGTTCCTGC AGAATCAGAA AGTCTGGTAA ATGGGAACCA CACCCCACAG ACTGCAACAC 
     1561 GGGGACCCTC TGCCTGTGCC AGCCACAGTT CCCTGGTGAG CTCTATTGAG AAGGACCTGC 
     1621 AAGAGATCAT GGACTCACTG GTGCTAGAGG AGCCTGGAGC TGCTGGCAAG AAGCCTGCCG 
     1681 CAACCTCTCC ACTGTCACCG ATGGCTAATG GTGGGCGCTA CCTGCTGTCT CCCCCAACCA 
     1741 GCCCCGGCGC CATGTCTGTG GGCTCCAGCT ATGAGAACAC CTCTCCAGCC TTCTCTCCAC 
     1801 TCTCTTCACC AGCCAGCAGT GGAAGCTGTG CCAGTCACTC ACCCAGTGGG CAAGAGCCAG 
     1861 GACCTTCTGT GCCCCCGCTG GTACCTGCCC GTTCCTCCAG CTACCATCTG GCCCTACAGC 
     1921 CCCCACAGTC CCGCCCAAGT GGTGCTCGCT CCGAGAGTCC TCGGCTGAGC AGGAAAGGGG 
     1981 GCCATGAGAG GCCTCCCAGC CCTGGCCTCC GGGGTCTGCT GACAGACAGC CCTGCAGCTA 
     2041 CTGTCTTGGC GGAGGCCCGG AGAGCCACTG AGAGCCCCCG GCTGGGTGGG CAGCTGCCTG 
     2101 TGGTGGCCAT CAGCCTGAGT GAATACCCAG CTTCTGGTGC TCTCAGTCAA CCCACCAGCA 
     2161 TTCCTGGCAG CCCCAAGTTT CAGCCTCCAG TCCCTGCTCC CCGAAACAAG ATTGGCACAC 
     2221 TCCAGGACCG CCCTCCCAGC CCTTTCCGTG AGCCTCCAGG CAGTGAGCGG GTGCTAACAA 
     2281 CCAGCCCCTC ACGCCAACTG GTGGGCCGAA CATTTTCAGA TGGGTTAGCC ACCCGTACCC 
     2341 TGCAGCCTCC TGAGAGTCCC CGCCTGGGCC GGCGGGGCCT GGACAGTATG CGAGAACTAC 
     2401 CCCCCTTAAG TCCATCTCTG TCCCGGCGAG CTCTCTCCCC GCTGCCCACC CGGACCACCC 
     2461 CAGATCCCAA GCTAAACAGG GAAGTGGCAG AGAGTCCTCG GCCCCGGCGC TGGGCAGCCC 
     2521 ATGGGGCTTC ACCAGAGGAC TTCTCCCTGA CGCTGGGGGC ACGGGGCCGT AGGACACGGA 
     2581 GCCCCTCACC CACACTGGGT GAGTCTCTGG CACCCCACAA GGGCAGCTTC AGTGGCAGGC 
     2641 TGAGCCCAGC CTACAGTCTG GGCTCTCTTA CTGGGGCTTC ACCCTGCCAG AGTCCCTGTG 
     2701 TCCAGAGGAA GCTCTCCAGC GGGGACTTGC GGGTGCCTGT CACAAGGGAG CGGAAAAATA 
     2761 GCATCACAGA GATCAGTGAC AATGAGGACG ACCTCCTGGA GTACCACCGG CGACAGCGCC 
     2821 AAGAGCGGCT CCGGGAGCAG GAGATGGAGA GGCTGGAACG CCAGCGCCTG GAGACCATCC 
     2881 TGAACCTGTG TGCCGAATAC AGCCGGGCTG ATGGGGGACC TGAGGCTGGG GAGCTTCCCA 
     2941 GCATTGGGGA GGCCACCGCA GCATTGGCAC TGGCAGGCCG GAGGCCCTCA CGAGGCCTTG 
     3001 CAGGGGCCTC TGGGCGGAGC AGCGAGGAGC CTGGCGTTGC CACCCAACGC CTATGGGAGA 
     3061 GTATGGAGCG CTCAGATGAG GAAAATCTCA AGGAGGAGTG CAGCAGCACT GAGAGCACCC 
     3121 AGCAGGAGCA CGAAGATGCA CCTAGCACCA AGCTCCAGGG AGAGGTGCTA GCCCTGGAAG 
     3181 AAGAGCGGGC TCAGGTGCTG GGGCACGTGG AGCAGCTCAA GGTCCGTGTG AAGGAGCTAG 
     3241 AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CCGAAATGGA GCGGGCACTG CTGCAGGGAG 
     3301 AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCAGTGGAT CAGCTGCAGG 
     3361 AGAAGCTGGT GGCCTTGGAG ACAGGCATCC AGAAGGAGAG GGACAAGGAG GCAGAGGCCC 
     3421 TGGAGACTGA GACAAAGCTC TTTGAGGACT TGGAGTTCCA GCAGTTGGAG CGGGAGAGCC 
     3481 GCGTGGAGGA GGAGCGCGAG CTGGCCGGCC AGGGGCTGCT CCGGAGCAAG GCTGAGCTGC 
     3541 TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGCCATCCT GGACAGTCAG GCTGGGCAGA 
     3601 TCCGGGCTCA GGCCGTGCAG GAGTCAGAAC GCCTGGCCCG GGACAAGAAT GCCTCCTTAC 
     3661 AGCTGCTGCA AAAGGAGAAG GAGAAGCTGA CTGTGCTGGA AAGGAGATAC CACTCACTCA 
     3721 CAGGGGGCAG GCCTTTCCCG AAGACCACAT CGACCCTCAA AGAGATGGAG AAGCTGCTGC 
     3781 TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG GGGACTGGCC 
     3841 CCGCTGCAGC CTCCCCTCAC TCTTCTCCCC CGCCTCTGCC CGCCAAAGCT TCCCGTCAGC 
     3901 TGCAGGTTTA CCGCTCCAAG ATGGATGGCG AGGCCACCAG CCCCCTTCCC CGGACCCGCA 
     3961 GCGGCCCCCT CCCCTCCTCC TCTGGCTCTT CCTCCTCCTC CTCCCAGCTC AGCGTGGCTA 
     4021 CCCTGGGGCG TAGCCCCTCC CCAAAGAGCG CTCTACTCAC CCAGAATGGC ACGGGCAGCC 
     4081 TTCCTCGCAA CCTGGCAGCC ACACTGCAGG ACATCGAGAC CAAGCGCCAA CTAGCTCTGC 
     4141 AGCAGAAGGG ACAACAAGTG ATTGAAGAGC AGCGGCGGCG ACTGGCTGAG CTGAAGCAGA 
     4201 AAGCGGCAGC TGAGGCACAG TGCCAGTGGG ATGCCCTTCA CGGGGCAGCA CCCTTCCCAG 
     4261 CGGGCCCCTC GGGCTTCCCC CCTCTCATGC ACCACTCTAT CCTACACCAC CTGCCTGCGG 
     4321 GGCGGGAGCG TGGGGAGGAG GGTGAGCACG CCTATGATAC GCTGAGTCTG GAGAGCTCTG 
     4381 ACAGCATGGA GACCAGCATC TCCACCGGGG GCAACTCGGC CTGCTCCCCT GACAACATGT 
     4441 CCAGCGCGAG TGGTCTGGAC ATGGGGAAGA TCGAGGAGAT GGAGAAGATG CTGAAAGAGG 
     4501 CTCATGCAGA GAAGAACCGG CTCATGGAGT CGAGGGAGCG GGAGATGGAG CTGCGGCGGC 
     4561 AGGCCCTGGA GGAGGAGCGG CGGAGGCGTG AGCAGGTAGA ACGGAGGCTG CAGAGTGAGA 
     4621 GTGCCCGGAG GCAGCAGCTG GTCGAGAAGG AGGTCAAGAT GCGGGAGAAA CAATTTTCCC 
     4681 AGGCACGACC CCTGACCCGC TACCTGCCAA TCCGGAAGGA GGACTTTGAC CTGAAGACAC 
     4741 ATATTGAGTC ATCGGGCCAT GGTGTTGATA CCTGCCTGCA CGTGGTGCTC AGCAGCAAGG 
     4801 TCTGCCGTGG CTACTTGGTC AAGATGGGCG GCAAGATTAA ATCATGGAAG AAGCGCTGGT 
     4861 TTGTCTTCGA CCGGCTCAAG CGCACCCTTT CCTATTATGT GGACAAGCAT GAGACGAAGC 
     4921 TGAAGGGAGT CATCTATTTC CAGGCCATTG AGGAAGTGTA CTACGACCAC CTGCGCAGTG 
     4981 CAGCCAAGAA GAGGTTTTTC CGCTTCACTA TGGTGACTGA GAGCCCGAAC CCAGCCCTCA 
     5041 CCTTCTGCGT AAAGACCCAT GACCGGCTGT ACTACATGGT GGCCCCATCT GCAGAGGCCA 
     5101 TGCGTATCTG GATGGATGTC ATTGTCACAG GGGCTGAGGG CTACACTCAG TTCATGAACA 
     5161 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     5221 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     5281 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     5341 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5401 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5461 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5521 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5581 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5641 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5701 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5761 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5821 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5881 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5941 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     6001 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     6061 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     6121 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     6181 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     6241 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     6301 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6361 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6421 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6481 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6541 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6601 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6661 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6721 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6781 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6841 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6901 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6961 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     7021 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     7081 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     7141 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     7201 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     7261 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     7321 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7381 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7441 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7501 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7561 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7621 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7681 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7741 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7801 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7861 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7921 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7981 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     8041 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     8101 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     8161 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     8221 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     8281 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     8341 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8401 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8461 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8521 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8581 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8641 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8701 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8761 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8821 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8881 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8941 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     9001 AACGCGAATT TT
//