LOCUS       RC214993                8229 bp    DNA     circular  SYN 
DEFINITION  EIF2AK3 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3)
ACCESSION   NM_004836
VERSION     NM_004836.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4386..4477 
                     /label="Myc-DDK"  
     misc_feature    4507..5092 
                     /label="PolyA signal"  
     misc_feature    4564..4583 
                     /label="Restriction Enzyme"  
     misc_feature    5368..6040 
                     /label="ColE1"  
     misc_feature    6470..7264 
                     /label="Kan/Neo"  
     misc_feature    7299..7601 
                     /label="SV40 ori"  
     misc_feature    7687..8164 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4368..4374 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4368 
                     /label="EIF2AK3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCGCGCC ATCAGCCCGG GGCTGCTGGT ACGGGCGCTG CTGCTGCTGC 
     1081 TGCTGCTGCT GGGGCTCGCG GCAAGGACGG TGGCCGCGGG GCGCGCCCGT GGCCTCCCAG 
     1141 CGCCGACGGC GGAGGCGGCG TTCGGCCTCG GGGCGGCCGC TGCTCCCACC TCAGCGACGC 
     1201 GAGTACCGGC GGCGGGCGCC GTGGCTGCGG CCGAGGTGAC TGTGGAGGAC GCTGAGGCGC 
     1261 TGCCGGCAGC CGCGGGAGAG CAGGAGCCTC GGGGTCCGGA ACCAGACGAT GAGACAGAGT 
     1321 TGCGACCGCG CGGCAGGTCA TTAGTAATTA TCAGCACTTT AGATGGGAGA ATTGCTGCCT 
     1381 TGGATCCTGA AAATCATGGT AAAAAGCAGT GGGATTTGGA TGTGGGATCC GGTTCCTTGG 
     1441 TGTCATCCAG CCTTAGCAAA CCAGAGGTAT TTGGGAATAA GATGATCATT CCTTCCCTGG 
     1501 ATGGAGCCCT CTTCCAGTGG GACCGAGACC GTGAAAGCAT GGAAACAGTT CCTTTCACAG 
     1561 TTGAATCACT TCTTGAATCT TCTTATAAAT TTGGAGATGA TGTTGTTTTG GTTGGAGGAA 
     1621 AATCTCTGAC TACATATGGA CTCAGTGCAT ATAGTGGAAA GGTGAGGTAT ATCTGTTCAG 
     1681 CTCTGGGTTG TCGCCAATGG GATAGTGACG AAATGGAACA AGAGGAAGAC ATCCTGCTTC 
     1741 TACAGCGTAC CCAAAAAACT GTTAGAGCTG TCGGACCTCG CAGTGGCAAT GAGAAGTGGA 
     1801 ATTTCAGTGT TGGCCACTTT GAACTTCGGT ATATTCCAGA CATGGAAACG AGAGCCGGAT 
     1861 TTATTGAAAG CACCTTTAAG CCCAATGAGA ACACAGAAGA GTCTAAAATT ATTTCAGATG 
     1921 TGGAAGAACA GGAAGCTGCC ATAATGGACA TAGTGATAAA GGTTTCGGTT GCTGACTGGA 
     1981 AAGTTATGGC ATTCAGTAAG AAGGGAGGAC ATCTGGAATG GGAGTACCAG TTTTGTACTC 
     2041 CAATTGCATC TGCCTGGTTA CTTAAGGATG GGAAAGTCAT TCCCATCAGT CTTTTTGATG 
     2101 ATACAAGTTA TACATCTAAT GATGATGTTT TAGAAGATGA AGAAGACATT GTAGAAGCTG 
     2161 CCAGAGGAGC CACAGAAAAC AGTGTTTACT TGGGAATGTA TAGAGGCCAG CTGTATCTGC 
     2221 AGTCATCAGT CAGAATTTCA GAAAAGTTTC CTTCAAGTCC CAAGGCTTTG GAATCTGTCA 
     2281 CTAATGAAAA CGCAATTATT CCTTTACCAA CAATCAAATG GAAACCCTTA ATTCATTCTC 
     2341 CTTCCAGAAC TCCTGTCTTG GTAGGATCTG ATGAATTTGA CAAATGTCTC AGTAATGATA 
     2401 AGTTTTCTCA TGAAGAATAT AGTAATGGTG CACTTTCAAT CTTGCAGTAT CCATATGATA 
     2461 ATGGTTATTA TCTACCATAC TACAAGAGGG AGAGGAACAA ACGAAGCACA CAGATTACAG 
     2521 TCAGATTCCT CGACAACCCA CATTACAACA AGAATATCCG CAAAAAGGAT CCTGTTCTTC 
     2581 TTTTACACTG GTGGAAAGAA ATAGTTGCAA CGATTTTGTT TTGTATCATA GCAACAACGT 
     2641 TTATTGTGCG CAGGCTTTTC CATCCTCATC CTCACAGGCA AAGGAAGGAG TCTGAAACTC 
     2701 AGTGTCAAAC TGAAAATAAA TATGATTCTG TAAGTGGTGA AGCCAATGAC AGTAGCTGGA 
     2761 ATGACATAAA AAACTCTGGA TATATATCAC GATATCTAAC TGATTTTGAG CCAATTCAAT 
     2821 GCCTGGGACG TGGTGGCTTT GGAGTTGTTT TTGAAGCTAA AAACAAAGTA GATGACTGCA 
     2881 ATTATGCTAT CAAGAGGATC CGTCTCCCCA ATAGGGAATT GGCTCGGGAA AAGGTAATGC 
     2941 GAGAAGTTAA AGCCTTAGCC AAGCTTGAAC ACCCGGGCAT TGTTAGATAT TTCAATGCCT 
     3001 GGCTCGAAGC ACCACCAGAG AAGTGGCAAG AAAAGATGGA TGAAATTTGG CTGAAAGATG 
     3061 AAAGCACAGA CTGGCCACTC AGCTCTCCTA GCCCAATGGA TGCACCATCA GTTAAAATAC 
     3121 GCAGAATGGA TCCTTTCTCT ACAAAAGAAC ATATTGAAAT CATAGCTCCT TCACCACAAA 
     3181 GAAGCAGGTC TTTTTCAGTA GGGATTTCCT GTGACCAGAC AAGTTCATCT GAGAGCCAGT 
     3241 TCTCACCACT GGAATTCTCA GGAATGGACC ATGAGGACAT CAGTGAGTCA GTGGATGCAG 
     3301 CATACAACCT CCAGGACAGT TGCCTTACAG ACTGTGATGT GGAAGATGGG ACTATGGATG 
     3361 GCAATGATGA GGGGCACTCC TTTGAACTTT GTCCTTCTGA AGCTTCTCCT TATGTAAGGT 
     3421 CAAGGGAGAG AACCTCCTCT TCAATAGTAT TTGAAGATTC TGGCTGTGAT AATGCTTCCA 
     3481 GTAAAGAAGA GCCGAAAACT AATCGATTGC ATATTGGCAA CCATTGTGCT AATAAACTAA 
     3541 CTGCTTTCAA GCCCACCAGT AGCAAATCTT CTTCTGAAGC TACATTGTCT ATTTCTCCTC 
     3601 CAAGACCAAC CACTTTAAGT TTAGATCTCA CTAAAAACAC CACAGAAAAA CTCCAGCCCA 
     3661 GTTCACCAAA GGTGTATCTT TACATTCAAA TGCAGCTGTG CAGAAAAGAA AACCTCAAAG 
     3721 ACTGGATGAA TGGACGATGT ACCATAGAGG AGAGAGAGAG GAGCGTGTGT CTGCACATCT 
     3781 TCCTGCAGAT CGCAGAGGCA GTGGAGTTTC TTCACAGTAA AGGACTGATG CACAGGGACC 
     3841 TCAAGCCATC CAACATATTC TTTACAATGG ATGATGTGGT CAAGGTTGGA GACTTTGGGT 
     3901 TAGTGACTGC AATGGACCAG GATGAGGAAG AGCAGACGGT TCTGACCCCA ATGCCAGCTT 
     3961 ATGCCAGACA CACAGGACAA GTAGGGACCA AACTGTATAT GAGCCCAGAG CAGATTCATG 
     4021 GAAACAGCTA TTCTCATAAA GTGGACATCT TTTCTTTAGG CCTGATTCTA TTTGAATTGC 
     4081 TGTATCCATT CAGCACTCAG ATGGAGAGAG TCAGGACCTT AACTGATGTA AGAAATCTCA 
     4141 AATTTCCACC ATTATTTACT CAGAAATATC CTTGTGAGTA CGTGATGGTT CAAGACATGC 
     4201 TCTCTCCATC CCCCATGGAA CGACCTGAAG CTATAAACAT CATTGAAAAT GCTGTATTTG 
     4261 AGGACTTGGA CTTTCCAGGA AAAACAGTGC TCAGACAGAG GTCTCGCTCC TTGAGTTCAT 
     4321 CGGGAACAAA ACATTCAAGA CAGTCCAACA ACTCCCATAG CCCTTTGCCA AGCAATACGC 
     4381 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4441 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4501 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4561 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4621 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4681 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4741 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4801 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4861 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4921 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4981 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5041 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5101 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5161 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5221 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5281 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5341 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5401 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5461 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5521 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5581 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5641 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5701 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5761 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5821 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5881 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5941 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6001 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6061 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6121 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6181 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6241 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6301 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6361 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6421 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6481 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6541 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6601 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6661 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6721 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6781 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6841 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6901 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6961 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7021 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7081 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7141 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7201 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7261 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7321 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7381 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7441 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7501 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7561 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7621 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7681 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7741 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7801 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7861 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7921 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7981 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8041 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8101 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8161 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8221 GCGAATTTT
//