LOCUS       RC214983                9060 bp    DNA     circular  SYN 
DEFINITION  RAB3GAP2 (Myc-DDK-tagged)-Human RAB3 GTPase activating protein subunit 2 (non-catalytic) (RAB3GAP2)
ACCESSION   NM_012414
VERSION     NM_012414.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5217..5308 
                     /label="Myc-DDK"  
     misc_feature    5338..5923 
                     /label="PolyA signal"  
     misc_feature    5395..5414 
                     /label="Restriction Enzyme"  
     misc_feature    6199..6871 
                     /label="ColE1"  
     misc_feature    7301..8095 
                     /label="Kan/Neo"  
     misc_feature    8130..8432 
                     /label="SV40 ori"  
     misc_feature    8518..8995 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5199..5205 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5199 
                     /label="RAB3GAP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCTGCTCC ATTGTCCAGT TCTGCTACTT CCAGGACCTC CAGGCCGCCC 
     1081 GGGACTTCCT CTTTCCTCAC CTGCGGGAGG AGATCCTCAG CGGCGCCTTG CGGAGGGACC 
     1141 CCAGTAAATC AACAGACTGG GAAGATGATG GTTGGGGAGC ATGGGAAGAA AATGAACCAC 
     1201 AAGAACCTGA AGAAGAAGGA AATACTTGCA AAACACAAAA AACTTCCTGG CTCCAAGATT 
     1261 GTGTTTTATC CTTATCTCCA ACCAATGATC TTATGGTGAT AGCTCGAGAG CAAAAAGCTG 
     1321 TATTTCTAGT GCCAAAATGG AAATATAGTG ATAAAGGAAA GGAAGAAATG CAATTTGCTG 
     1381 TTGGCTGGAG TGGTTCCTTA AATGTCGAAG AAGGGGAATG TGTAACCAGT GCTCTATGTA 
     1441 TCCCACTAGC AAGCCAAAAG AGGAGTTCCA CTGGGCGTCC TGACTGGACC TGCATTGTGG 
     1501 TGGGTTTTAC TTCAGGTTAT GTACGCTTCT ACACTGAGAA TGGTGTGCTC TTGCTTGCAC 
     1561 AGCTTTTGAA TGAGGACCCA GTACTTCAAC TTAAATGCAG AACCTATGAA ATACCACGAC 
     1621 ATCCCGGCGT GACTGAGCAG AATGAAGAGT TGAGTATCTT ATATCCAGCT GCCATTGTGA 
     1681 CTATTGATGG ATTTAGCCTT TTTCAATCTC TTCGTGCTTG TCGAAATCAG GTAGCAAAAG 
     1741 CTGCAGCATC AGGCAATGAG AACATACAAC CACCACCATT AGCTTATAAG AAATGGGGTC 
     1801 TACAAGATAT TGACACTATT ATTGATCATG CTAGTGTTGG TATTATGACT CTGTCCCCCT 
     1861 TTGATCAAAT GAAGACTGCC TCCAATATAG GTGGATTTAA TGCAGCAATT AAAAATAGTC 
     1921 CACCTGCCAT GTCTCAGTAT ATCACTGTAG GGTCCAATCC ATTTACTGGC TTCTTCTATG 
     1981 CTTTAGAGGG AAGCACACAA CCATTATTAT CCCATGTTGC ACTAGCAGTT GCAAGTAAAC 
     2041 TCACTTCTGC TTTATTTAAT GCTGCCAGTG GTTGGCTTGG TTGGAAAAGT AAGCACGAAG 
     2101 AAGAAGCTGT CCAAAAGCAA AAGCCGAAGG TTGAGCCAGC TACCCCATTA GCTGTAAGAT 
     2161 TTGGACTTCC AGATTCTAGA CGCCATGGTG AAAGCATATG TCTGTCTCCA TGTAACACAC 
     2221 TGGCAGCAGT AACAGATGAT TTCGGCAGAG TTATTTTATT GGATGTAGCT AGAGGAATTG 
     2281 CAATACGCAT GTGGAAAGGG TACCGCGACG CACAAATTGG ATGGATTCAA ACTGTAGAGG 
     2341 ACCTCCATGA AAGAGTGCCA GAAAAGGCAG ATTTTTCCCC CTTTGGAAAT TCTCAGGGTC 
     2401 CAAGTCGAGT AGCTCAATTC CTTGTGATCT ATGCGCCAAG AAGGGGAATT TTAGAAGTGT 
     2461 GGAGCACACA GCAGGGACCT AGAGTAGGAG CTTTCAATGT GGGGAAGCAC TGCAGGCTGC 
     2521 TGTATCCTGG CTATAAAATA ATGGGTTTAA ATAATGTTAC CAGTCAGAGT TGGCAGCCAC 
     2581 AGACTTATCA GATCTGTCTG GTTGATCCAG TGTCTGGAAG TGTGAAAACA GTGAACGTTC 
     2641 CCTTCCATTT AGCACTGAGT GATAAGAAGA GTGAACGAGC CAAGGATATG CACCTAGTGA 
     2701 AGAAACTAGC AGCCTTACTG AAAACAAAAT CTCCCAATCT TGATTTGGTT GAAACAGAAA 
     2761 TAAAGGAATT AATTCTTGAT ATTAAATACC CTGCAACCAA AAAACAAGCT TTGGAAAGCA 
     2821 TTTTGGCAAG TGAACGTTTA CCATTTTCTT GCCTTAGAAA CATCACTCAG ACTTTAATGG 
     2881 ACACTTTAAA AAGTCAAGAA CTTGAGTCTG TTGATGAAGG ATTGCTACAG TTTTGTGCCA 
     2941 ATAAACTAAA ACTGCTGCAA CTCTATGAGT CTGTCAGTCA ATTAAATTCC CTTGATTTTC 
     3001 ATTTAGACAC ACCATTCTCT GATAATGACT TGGCTCTGTT ACTAAGGCTT GATGAAAAAG 
     3061 AACTGCTTAA GCTCCAGGCA TTACTAGAGA AATATAAGCA AGAGAACACC AGGACAAATG 
     3121 TTCGATTTTC TGATGATAAA GATGGTGTGT TGCCTGTAAA AACATTCTTG GAATATTTAG 
     3181 AATATGAAAA GGATGTGCTC AACATAAAGA AAATAAGTGA AGAGGAATAT GTGGCTTTAG 
     3241 GTAGTTTCTT TTTTTGGAAG TGTTTGCATG GAGAAAGCTC CACTGAGGAT ATGTGTCACA 
     3301 CTTTGGAGTC GGCTGGTCTT AGCCCTCAGC TGTTGTTGTC TCTGCTCCTG AGTGTTTGGC 
     3361 TTTCAAAGGA AAAGGATATT TTGGATAAAC CACAGTCAAT CTGCTGTCTT CATACCATGC 
     3421 TGTCCCTCCT GAGCAAGATG AAAGTGGCCA TCGATGAGAC CTGGGATTCT CAGTCTGTGT 
     3481 CCCCATGGTG GCAGCAGATG CGCACAGCCT GTATTCAGTC TGAGAACAAT GGAGCCGCTC 
     3541 TGTTGTCTGC GCATGTTGGG CATTCTGTTG CTGCACAGAT ATCAAACAAC ATGACAGAGA 
     3601 AAAAATTTTC CCAAACAGTT TTGGGTGCTG ATTCAGAGGC CCTCACTGAT TCCTGGGAGG 
     3661 CACTTTCTCT TGACACTGAG TACTGGAAAC TCCTTCTGAA ACAGCTGGAG GATTGTCTCA 
     3721 TACTTCAGAC TCTGCTTCAC AGCAAAGGGA ACACTCAGAC CTCCAAAGTG TCATCACTGC 
     3781 AGGCTGAGCC ACTTCCAAGG CTTTCTGTTA AAAAGTTATT AGAAGGAGGA AAAGGTGGCA 
     3841 TTGCAGACAG TGTAGCCAAG TGGATATTTA AACAGGACTT CAGCCCTGAA GTATTAAAAC 
     3901 TGGCTAATGA AGAAAGAGAT GCAGAAAACC CAGATGAACC CAAAGAAGGT GTTAACAGAA 
     3961 GTTTCCTTGA GGTATCAGAG ATGGAGATGG ACTTAGGAGC CATACCAGAC TTACTGCATT 
     4021 TAGCCTATGA GCAGTTTCCT TGTAGCCTTG AGCTCGATGT CTTGCATGCA CATTGCTGCT 
     4081 GGGAGTACGT TGTTCAGTGG AATAAAGATC CAGAGGAAGC ACGTTTTTTT GTTAGGTCAA 
     4141 TAGAACACTT GAAGCAAATA TTTAATGCAC ATGTTCAAAA TGGCATTGCA CTGATGATGT 
     4201 GGAATACGTT CTTAGTTAAA AGATTTTCTG CTGCTACATA CTTAATGGAT AAGGTTGGAA 
     4261 AATCACCAAA AGATAGGTTA TGCCGAAGGG ATGTGGGAAT GAGTGACACA GCAATGACAT 
     4321 CTTTCCTCGG CTCCTGTTTG GATCTTCTTC AGATCTTAAT GGAGGCAGAT GTTAGCAGGG 
     4381 ATGAAATACA GGTGCCTGTG CTGGATACTG AGGATGCGTG GCTCTCCGTG GAAGGACCAA 
     4441 TCTCCATAGT GGAACTGGCC CTTGAACAGA AGCACATCCA CTACCCACTG GTGGAGCACC 
     4501 ACTCCATCCT GTGCTCCATC TTGTATGCAG TCATGAGGTT TTCTCTGAAG ACCGTGAAGC 
     4561 CACTTTCACT TTTTGACAGT AAGGGAAAAA ATGCATTTTT CAAAGACCTA ACTTCAATTC 
     4621 AGTTATTACC TAGTGGGGAA ATGGATCCAA ATTTTATTTC TGTACGACAA CAGTTCTTAT 
     4681 TGAAAGTTGT CAGTGCAGCT GTCCAGGCCC AACATTCAGC CACAAAGGTC AAAGATCCCA 
     4741 CAGAAGAGGC CACACCCACT CCTTTTGGGA AAGACCAAGA TTGGCCAGCT CTAGCTGTGG 
     4801 ATTTAGCCCA TCACCTTCAA GTTAGTGAAG ATGTTGTTAG AAGGCATTAT GTGGGGGAAC 
     4861 TATACAACTA TGGAGTTGAC CACTTAGGAG AAGAGGCCAT TCTACAGGTT CATGACAAAG 
     4921 AGGTCCTTGC CTCTCAGCTG CTGGTGCTCA CGGGGCAAAG GCTGGCTCAT GCGCTTCTCC 
     4981 ACACCCAGAC AAAAGAAGGA ATGGAGCTGC TTGCCAGACT TCCACCCACA CTGTGTACTT 
     5041 GGCTGAAAGC AATGGACCCC CAGGACCTTC AAAACACTGA AGTGCCAATT GCAACAACAG 
     5101 CTAAACTAGT AAATAAAGTA ATTGAGCTTT TACCAGAAAA ACATGGGCAA TATGGTCTAG 
     5161 CCTTACACCT CATTGAAGCT GTGGAAGCCA TATCTCTTCC TTCTTTAACG CGTACGCGGC 
     5221 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     5281 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     5341 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     5401 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5461 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5521 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5581 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5641 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5701 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5761 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5821 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5881 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5941 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     6001 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     6061 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     6121 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     6181 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     6241 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     6301 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     6361 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6421 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6481 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6541 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6601 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6661 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6721 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6781 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6841 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6901 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6961 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     7021 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     7081 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     7141 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     7201 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     7261 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     7321 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     7381 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7441 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7501 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7561 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7621 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7681 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7741 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7801 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7861 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7921 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7981 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     8041 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     8101 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     8161 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     8221 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     8281 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     8341 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     8401 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8461 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8521 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8581 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8641 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8701 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8761 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8821 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8881 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8941 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     9001 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//