LOCUS       RC214974L4                8788 bp    DNA     circular  SYN 
DEFINITION  ETNK2 (Myc-DDK-tagged)-Human ethanolamine kinase 2 (ETNK2)
ACCESSION   NM_018208
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7079..7259 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    7987..8633 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    5954..5974 
                     /label="LR50"  
     misc_feature    4417..5145 
                     /label="mGFP"  
     misc_feature    5152..5217 
                     /label="P2A"  
     misc_feature    7307..7926 
                     /label="pBR322 Ori"  
     misc_feature    5218..5817 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    4396..4402 
                     /label="Restriction Enzyme"  
     misc_feature    3320..4396 
                     /label="ETNK2"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCTGTGCCC CCTTCGGCCC CTCAGCCGCG 
     3361 CGCGTCCTTT CACCTGAGGA GGCACACGCC TTGCCCGCAG TGCTCATGGG GCATGGAGGA 
     3421 GAAGGCGGCG GCCAGCGCCA GCTGCCGGGA GCCGCCGGGC CCCCCGAGGG CCGCCGCCGT 
     3481 CGCGTACTTC GGCATTTCCG TGGACCCGGA CGACATCCTT CCCGGGGCCC TGCGCCTCAT 
     3541 CCAGGAGCTG CGGCCGCATT GGAAACCCGA GCAAGTTCGG ACCAAGCGCT TCACGGATGG 
     3601 CATCACCAAC AAGCTGGTGG CCTGCTATGT GGAGGAGGAC ATGCAGGACT GCGTGCTGGT 
     3661 CCGGGTGTAT GGGGAGCGGA CGGAGCTGCT GGTGGACCGG GAGAATGAGG TCAGAAACTT 
     3721 CCAGCTGCTG CGAGCACACA GCTGTGCCCC CAAACTCTAC TGCACCTTCC AGAATGGGCT 
     3781 GTGCTATGAG TACATGCAGG GTGTGGCCCT GGAGCCTGAG CACATCCGTG AGCCCCGGCT 
     3841 TTTCAGGTTA ATCGCCTTAG AAATGGCAAA GATTCATACT ATCCACGCCA ACGGCAGCCT 
     3901 GCCCAAGCCC ATCCTCTGGC ACAAGATGCA CAATTATTTC ACGCTTGTGA AGAACGAGAT 
     3961 CAACCCCAGC CTTTCTGCAG ATGTCCCTAA GGTAGAGGTG TTGGAACGGG AGCTGGCCTG 
     4021 GCTGAAGGAG CATCTGTCCC AGCTGGAGTC CCCTGTGGTG TTTTGTCACA ATGACCTGCT 
     4081 CTGCAAGAAT ATCATCTATG ACAGCATCAA AGGTCACGTG CGGTTCATTG ACTATGAATA 
     4141 TGCTGGCTAC AACTACCAAG CTTTTGACAT TGGCAACCAT TTCAATGAGT TTGCAGGCGT 
     4201 GAATGAGGTG GATTACTGCC TGTACCCGGC GCGGGAGACC CAGCTGCAGT GGCTGCACTA 
     4261 CTACCTGCAG ACACAAAAGG GGATGGCCGT GACCCCCAGG GAGGTGCAAA GGCTCTACGT 
     4321 GCAAGTCAAC AAGTTTGCCC TGGCGTCTCA CTTCTTCTGG GCTCTCTGGG CCCTCATCCA 
     4381 GAACCAGTAC TCCACCATCG ACTTACGCGT ACGCGGCCGC TCGAGATGAG CGGGGGCGAG 
     4441 GAGCTGTTCG CCGGCATCGT GCCCGTGCTG ATCGAGCTGG ACGGCGACGT GCACGGCCAC 
     4501 AAGTTCAGCG TGCGCGGCGA GGGCGAGGGC GACGCCGACT ACGGCAAGCT GGAGATCAAG 
     4561 TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTGGTGAC CACCCTCTGC 
     4621 TACGGCATCC AGTGCTTCGC CCGCTACCCC GAGCACATGA AGATGAACGA CTTCTTCAAG 
     4681 AGCGCCATGC CCGAGGGCTA CATCCAGGAG CGCACCATCC AGTTCCAGGA CGACGGCAAG 
     4741 TACAAGACCC GCGGCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG 
     4801 AAGGGCAAGG ACTTCAAGGA GGACGGCAAC ATCCTGGGCC ACAAGCTGGA GTACAGCTTC 
     4861 AACAGCCACA ACGTGTACAT CCGCCCCGAC AAGGCCAACA ACGGCCTGGA GGCTAACTTC 
     4921 AAGACCCGCC ACAACATCGA GGGCGGCGGC GTGCAGCTGG CCGACCACTA CCAGACCAAC 
     4981 GTGCCCCTGG GCGACGGCCC CGTGCTGATC CCCATCAACC ACTACCTGAG CACTCAGACC 
     5041 AAGATCAGCA AGGACCGCAA CGAGGCCCGC GACCACATGG TGCTCCTGGA GTCCTTCAGC 
     5101 GCCTGCTGCC ACACCCACGG CATGGACGAG CTGTACAGGT CCGGACTCAG AGTTTGGGTA 
     5161 GGAAGCGGAG CTACTAACTT CAGCCTGCTG AAGCAGGCTG GAGACGTGGA GGAGAACCCT 
     5221 GGACCTATGA CCGAGTACAA GCCCACGGTG CGCCTCGCCA CCCGCGACGA CGTCCCCAGG 
     5281 GCCGTACGCA CCCTCGCCGC CGCGTTCGCC GACTACCCCG CCACGCGCCA CACCGTCGAT 
     5341 CCGGATCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG 
     5401 CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG 
     5461 CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG 
     5521 AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC 
     5581 AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTCTCGC CCGACCACCA GGGCAAGGGT 
     5641 CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC 
     5701 TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG AGCGGCTCGG CTTCACCGTC 
     5761 ACCGCCGACG TCGAGGTGCC CGAAGGACCG CGCACCTGGT GCATGACCCG CAAGCCCGGT 
     5821 GCCTGATGTA CACCCAAACG GCCGGCCGCG GTCTGTACAA GTAGGATTCG TCGAGGGACC 
     5881 TAATAACTTC GTATAGCATA CATTATACGA AGTTATACAT GTTTAAGGGT TCCGGTTCCA 
     5941 CTAGGTACAA TTCGATATCA AGCTTATCGA TAATCAACCT CTGGATTACA AAATTTGTGA 
     6001 AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT 
     6061 AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA 
     6121 ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT 
     6181 GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT 
     6241 CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG 
     6301 CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC 
     6361 GGGGAAATCA TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG 
     6421 GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT 
     6481 GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC 
     6541 CCTTTGGGCC GCCTCCCCGC ATCGATACCG TCGACCTCGA TCGAGACCTA GAAAAACATG 
     6601 GAGCAATCAC AAGTAGCAAT ACAGCAGCTA CCAATGCTGA TTGTGCCTGG CTAGAAGCAC 
     6661 AAGAGGAGGA GGAGGTGGGT TTTCCAGTCA CACCTCAGGT ACCTTTAAGA CCAATGACTT 
     6721 ACAAGGCAGC TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG GAAGGGCTAA 
     6781 TTCACTCCCA ACGAAGACAA GATATCCTTG ATCTGTGGAT CTACCACACA CAAGGCTACT 
     6841 TCCCTGATTG GCAGAACTAC ACACCAGGGC CAGGGATCAG ATATCCACTG ACCTTTGGAT 
     6901 GGTGCTACAA GCTAGTACCA GTTGAGCAAG AGAAGGTAGA AGAAGCCAAT GAAGGAGAGA 
     6961 ACACCCGCTT GTTACACCCT GTGAGCCTGC ATGGGATGGA TGACCCGGAG AGAGAAGTAT 
     7021 TAGAGTGGAG GTTTGACAGC CGCCTAGCAT TTCATCACAT GGCCCGAGAG CTGCATCCGG 
     7081 ACTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG 
     7141 GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC 
     7201 GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA 
     7261 TCTCTAGCAG CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT 
     7321 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 
     7381 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT 
     7441 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 
     7501 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG 
     7561 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT 
     7621 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 
     7681 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA 
     7741 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA 
     7801 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG 
     7861 GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 
     7921 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 
     7981 GGATTTTGGT CATGATTACG CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT 
     8041 AAGCATTCTG CCGACATGGA AGCCATCACA AACGGCATGA TGAACCTGAA TCGCCAGCGG 
     8101 CATCAGCACC TTGTCGCCTT GCGTATAATA TTTGCCCATG GTGAAAACGG GGGCGAAGAA 
     8161 GTTGTCCATA TTGGCCACGT TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA 
     8221 GACGAAAAAC ATATTCTCAA TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA 
     8281 CGCCACATCT TGCGAATATA TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA 
     8341 GAGCGATGAA AACGTTTCAG TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC 
     8401 CCATATCACC AGCTCACCGT CTTTCATTGC CATACGGAAC TCCGGATGAG CATTCATCAG 
     8461 GCGGGCAAGA ATGTGAATAA AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT 
     8521 TAAAAAGGCC GTAATATCCA GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG 
     8581 AAATGCCTCA AAATGTTCTT TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT 
     8641 GATTTTTTTC TCCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG 
     8701 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG 
     8761 CACATTTCCC CGAAAAGTGC CACCTGAC
//