LOCUS       RC214949                8784 bp    DNA     circular  SYN 
DEFINITION  EHMT1 (Myc-DDK-tagged)-Human euchromatic histone-lysine N-methyltransferase 1 (EHMT1), transcript variant 1
ACCESSION   NM_024757
VERSION     NM_024757.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4939..5030 
                     /label="Myc-DDK"  
     misc_feature    5060..5645 
                     /label="PolyA signal"  
     misc_feature    5117..5136 
                     /label="Restriction Enzyme"  
     misc_feature    5921..6593 
                     /label="ColE1"  
     misc_feature    7023..7817 
                     /label="Kan/Neo"  
     misc_feature    7852..8154 
                     /label="SV40 ori"  
     misc_feature    8240..8717 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4914..4921 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4914 
                     /label="EHMT1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGCCGCC GATGCCGAGG CAGTTCCGGC GAGGGGGGAG CCTCAGCAGG 
     1081 ATTGCTGTGT GAAAACCGAG CTGCTGGGAG AAGAGACACC TATGGCTGCC GATGAAGGCT 
     1141 CAGCAGAGAA ACAGGCAGGA GAGGCCCACA TGGCTGCGGA CGGTGAGACC AATGGGTCTT 
     1201 GTGAAAACAG CGATGCCAGC AGTCATGCAA ATGCTGCAAA GCACACTCAG GACAGCGCAA 
     1261 GGGTCAACCC CCAGGATGGC ACCAACACAC TAACTCGGAT AGCGGAAAAT GGGGTTTCAG 
     1321 AAAGAGACTC AGAAGCGGCG AAGCAAAACC ACGTCACTGC CGACGACTTT GTGCAGACTT 
     1381 CTGTCATCGG CAGCAACGGA TACATCTTAA ATAAGCCGGC CCTACAGGCA CAGCCCTTGA 
     1441 GGACTACCAG CACTCTGGCC TCTTCGCTGC CTGGCCATGC TGCAAAAACC CTTCCTGGAG 
     1501 GGGCTGGCAA AGGCAGGACT CCAAGCGCTT TTCCCCAGAC GCCAGCCGCC CCACCAGCCA 
     1561 CCCTTGGGGA GGGGAGTGCT GACACAGAGG ACAGGAAGCT CCCGGCCCCT GGCGCCGACG 
     1621 TCAAGGTCCA CAGGGCACGC AAGACCATGC CGAAGTCCGT CGTGGGCCTG CATGCAGCCA 
     1681 GTAAAGATCC CAGAGAAGTT CGAGAAGCTA GAGATCATAA GGAACCAAAA GAGGAGATCA 
     1741 ACAAAAACAT TTCTGACTTT GGACGACAGC AGCTTTTACC CCCCTTCCCA TCCCTTCATC 
     1801 AGTCGCTACC TCAGAACCAG TGCTACATGG CCACCACAAA ATCACAGACA GCTTGCTTGC 
     1861 CTTTTGTTTT AGCAGCTGCA GTATCTCGGA AGAAAAAACG AAGAATGGGA ACCTATAGCC 
     1921 TGGTTCCTAA GAAAAAGACC AAAGTATTAA AACAGAGGAC GGTGATTGAG ATGTTTAAGA 
     1981 GCATAACTCA TTCCACTGTG GGTTCCAAGG GGGAGAAGGA CCTGGGCGCC AGCAGCCTGC 
     2041 ACGTGAATGG GGAGAGCCTG GAGATGGACT CGGATGAGGA CGACTCAGAG GAGCTCGAGG 
     2101 AGGACGACGG CCATGGTGCA GAGCAGGCGG CCGCGTTCCC CACAGAGGAC AGCAGGACTT 
     2161 CCAAGGAGAG CATGTCGGAG GCTGATCGCG CCCAGAAGAT GGACGGGGAG TCCGAGGAGG 
     2221 AGCAGGAGTC CGTGGACACC GGGGAGGAGG AGGAAGGCGG TGACGAGTCT GACCTGAGTT 
     2281 CGGAATCCAG CATTAAGAAG AAATTTCTCA AGAGGAAAGG AAAGACCGAC AGTCCCTGGA 
     2341 TCAAGCCAGC CAGGAAAAGG AGGCGGAGAA GTAGAAAGAA GCCCAGCGGT GCCCTCGGTT 
     2401 CTGAGTCGTA TAAGTCATCT GCAGGAAGCG CTGAGCAGAC GGCACCAGGA GACAGCACAG 
     2461 GGTACATGGA AGTTTCTCTG GACTCCCTGG ATCTCCGAGT CAAAGGAATT CTGTCTTCAC 
     2521 AAGCAGAAGG GTTGGCCAAC GGTCCAGATG TGCTGGAGAC AGACGGCCTC CAGGAAGTGC 
     2581 CTCTCTGCAG CTGCCGGATG GAAACACCGA AGAGTCGAGA GATCACCACA CTGGCCAACA 
     2641 ACCAGTGCAT GGCTACAGAG AGCGTGGACC ATGAATTGGG CCGGTGCACA AACAGCGTGG 
     2701 TCAAGTATGA GCTGATGCGC CCCTCCAACA AGGCCCCGCT CCTCGTGCTG TGTGAAGACC 
     2761 ACCGGGGCCG CATGGTGAAG CACCAGTGCT GTCCTGGCTG TGGCTACTTC TGCACAGCGG 
     2821 GTAATTTTAT GGAGTGTCAG CCCGAGAGCA GCATCTCTCA CCGTTTCCAC AAAGACTGTG 
     2881 CCTCTCGAGT CAATAACGCC AGCTATTGTC CCCACTGTGG GGAGGAGAGC TCCAAGGCCA 
     2941 AAGAGGTGAC GATAGCTAAA GCAGACACCA CCTCGACCGT GACACCAGTC CCCGGGCAGG 
     3001 AGAAGGGCTC GGCCCTGGAG GGCAGGGCCG ACACCACAAC GGGCAGTGCT GCCGGGCCAC 
     3061 CACTCTCGGA GGACGACAAG CTGCAGGGTG CAGCCTCCCA CGTGCCCGAG GGCTTTGATC 
     3121 CAACGGGACC TGCTGGGCTT GGGAGGCCAA CTCCCGGCCT TTCCCAGGGA CCAGGGAAGG 
     3181 AAACCTTGGA GAGCGCTCTC ATCGCCCTCG ACTCGGAAAA ACCCAAGAAG CTTCGCTTCC 
     3241 ACCCAAAGCA GCTGTACTTC TCCGCCAGGC AAGGGGAGCT TCAGAAGGTG CTCCTCATGC 
     3301 TGGTGGACGG AATTGACCCC AACTTCAAAA TGGAGCACCA GAATAAGCGC TCTCCACTGC 
     3361 ACGCCGCGGC AGAGGCTGGA CACGTGGACA TCTGCCACAT GCTGGTTCAG GCGGGCGCTA 
     3421 ATATTGACAC CTGCTCAGAA GACCAGAGGA CCCCGTTGAT GGAAGCAGCC GAAAACAACC 
     3481 ATCTGGAAGC AGTGAAGTAC CTCATCAAGG CTGGGGCCCT GGTGGATCCC AAGGACGCAG 
     3541 AGGGCTCTAC GTGTTTGCAC CTGGCTGCCA AGAAAGGCCA CTACGAAGTG GTCCAGTACC 
     3601 TGCTTTCAAA TGGACAGATG GACGTCAACT GTCAGGATGA CGGAGGCTGG ACACCCATGA 
     3661 TCTGGGCCAC AGAGTACAAG CACGTGGACC TCGTGAAGCT GCTGCTGTCC AAGGGCTCTG 
     3721 ACATCAACAT CCGAGACAAC GAGGAGAACA TTTGCCTGCA CTGGGCGGCG TTCTCCGGCT 
     3781 GCGTGGACAT AGCCGAGATC CTGCTGGCTG CCAAGTGCGA CCTCCACGCC GTGAACATCC 
     3841 ACGGAGACTC GCCACTGCAC ATTGCCGCCC GGGAGAACCG CTACGACTGT GTCGTCCTCT 
     3901 TTCTTTCTCG GGATTCAGAT GTCACCTTAA AGAACAAGGA AGGAGAGACG CCCCTGCAGT 
     3961 GTGCGAGCCT CAACTCTCAG GTGTGGAGCG CTCTGCAGAT GAGCAAGGCT CTGCAGGACT 
     4021 CGGCCCCCGA CAGGCCCAGC CCCGTGGAGA GGATAGTGAG CAGGGACATC GCTCGAGGCT 
     4081 ACGAGCGCAT CCCCATCCCC TGTGTCAACG CCGTGGACAG CGAGCCATGC CCCAGCAACT 
     4141 ACAAGTACGT CTCTCAGAAC TGCGTGACGT CCCCCATGAA CATCGACAGA AATATCACTC 
     4201 ATCTGCAGTA CTGCGTGTGC ATCGACGACT GCTCCTCCAG CAACTGCATG TGCGGCCAGC 
     4261 TCAGCATGCG CTGCTGGTAC GACAAGGATG GCCGGCTCCT GCCAGAGTTC AACATGGCGG 
     4321 AGCCTCCCTT GATCTTCGAA TGCAACCACG CGTGCTCCTG CTGGAGGAAC TGCCGAAATC 
     4381 GCGTCGTACA GAATGGTCTC AGGGCAAGGC TGCAGCTCTA CCGGACGCGG GACATGGGCT 
     4441 GGGGCGTGCG GTCCCTGCAG GACATCCCAC CAGGCACCTT TGTCTGCGAG TATGTTGGGG 
     4501 AGCTGATTTC AGACTCAGAA GCCGACGTTC GAGAGGAAGA TTCTTACCTC TTTGATCTCG 
     4561 ACAATAAGGA CGGGGAGGTT TACTGCATCG ACGCGCGGTT CTACGGGAAC GTCAGCCGGT 
     4621 TCATCAACCA CCACTGCGAG CCCAACCTGG TGCCCGTGCG CGTGTTCATG GCCCACCAGG 
     4681 ACCTGCGGTT CCCCCGGATC GCCTTCTTCA GCACCCGCCT GATCGAGGCC GGCGAGCAGC 
     4741 TCGGGTTTGA CTATGGAGAG CGCTTCTGGG ACATCAAAGG CAAGCTCTTC AGCTGCCGCT 
     4801 GCGGCTCCCC CAAGTGCCGG CACTCGAGCG CGGCCCTGGC CCAGCGTCAG GCCAGCGCGG 
     4861 CCCAGGAGGC CCAGGAGGAC GGCTTGCCCG ACACCAGCTC CGCGGCTGCC GCCGACCCCC 
     4921 TAAGCGGACC GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4981 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5041 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5101 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5161 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5221 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5281 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5341 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5401 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5461 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5521 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5581 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5641 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5701 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5761 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5821 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5881 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5941 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6001 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6061 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6121 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6181 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6241 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6301 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6361 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6421 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6481 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6541 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6601 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6661 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6721 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6781 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6841 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6901 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6961 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7021 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7081 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7141 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7201 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7261 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7321 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7381 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7441 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7501 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7561 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7621 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7681 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7741 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7801 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7861 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7921 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7981 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8041 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8101 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8161 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8221 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8281 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8341 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8401 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8461 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8521 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8581 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8641 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8701 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8761 TAACAAAAAT TTAACGCGAA TTTT
//