LOCUS       RC214928                8982 bp    DNA     circular  SYN 
DEFINITION  IGF1R (Myc-DDK-tagged)-Human insulin-like growth factor 1 receptor (IGF1R)
ACCESSION   NM_000875
VERSION     NM_000875.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5139..5230 
                     /label="Myc-DDK"  
     misc_feature    5260..5845 
                     /label="PolyA signal"  
     misc_feature    5317..5336 
                     /label="Restriction Enzyme"  
     misc_feature    6121..6793 
                     /label="ColE1"  
     misc_feature    7223..8017 
                     /label="Kan/Neo"  
     misc_feature    8052..8354 
                     /label="SV40 ori"  
     misc_feature    8440..8917 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5121..5127 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5121 
                     /label="IGF1R"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGTCTGGC TCCGGAGGAG GGTCCCCGAC CTCGCTGTGG GGGCTCCTGT 
     1081 TTCTCTCCGC CGCGCTCTCG CTCTGGCCGA CGAGTGGAGA AATCTGCGGG CCAGGCATCG 
     1141 ACATCCGCAA CGACTATCAG CAGCTGAAGC GCCTGGAGAA CTGCACGGTG ATCGAGGGCT 
     1201 ACCTCCACAT CCTGCTCATC TCCAAGGCCG AGGACTACCG CAGCTACCGC TTCCCCAAGC 
     1261 TCACGGTCAT TACCGAGTAC TTGCTGCTGT TCCGAGTGGC TGGCCTCGAG AGCCTCGGAG 
     1321 ACCTCTTCCC CAACCTCACG GTCATCCGCG GCTGGAAACT CTTCTACAAC TACGCCCTGG 
     1381 TCATCTTCGA GATGACCAAT CTCAAGGATA TTGGGCTTTA CAACCTGAGG AACATTACTC 
     1441 GGGGGGCCAT CAGGATTGAG AAAAATGCTG ACCTCTGTTA CCTCTCCACT GTGGACTGGT 
     1501 CCCTGATCCT GGATGCGGTG TCCAATAACT ACATTGTGGG GAATAAGCCC CCAAAGGAAT 
     1561 GTGGGGACCT GTGTCCAGGG ACCATGGAGG AGAAGCCGAT GTGTGAGAAG ACCACCATCA 
     1621 ACAATGAGTA CAACTACCGC TGCTGGACCA CAAACCGCTG CCAGAAAATG TGCCCAAGCA 
     1681 CGTGTGGGAA GCGGGCGTGC ACCGAGAACA ATGAGTGCTG CCACCCCGAG TGCCTGGGCA 
     1741 GCTGCAGCGC GCCTGACAAC GACACGGCCT GTGTAGCTTG CCGCCACTAC TACTATGCCG 
     1801 GTGTCTGTGT GCCTGCCTGC CCGCCCAACA CCTACAGGTT TGAGGGCTGG CGCTGTGTGG 
     1861 ACCGTGACTT CTGCGCCAAC ATCCTCAGCG CCGAGAGCAG CGACTCCGAG GGGTTTGTGA 
     1921 TCCACGACGG CGAGTGCATG CAGGAGTGCC CCTCGGGCTT CATCCGCAAC GGCAGCCAGA 
     1981 GCATGTACTG CATCCCTTGT GAAGGTCCTT GCCCGAAGGT CTGTGAGGAA GAAAAGAAAA 
     2041 CAAAGACCAT TGATTCTGTT ACTTCTGCTC AGATGCTCCA AGGATGCACC ATCTTCAAGG 
     2101 GCAATTTGCT CATTAACATC CGACGGGGGA ATAACATTGC TTCAGAGCTG GAGAACTTCA 
     2161 TGGGGCTCAT CGAGGTGGTG ACGGGCTACG TGAAGATCCG CCATTCTCAT GCCTTGGTCT 
     2221 CCTTGTCCTT CCTAAAAAAC CTTCGCCTCA TCCTAGGAGA GGAGCAGCTA GAAGGGAATT 
     2281 ACTCCTTCTA CGTCCTCGAC AACCAGAACT TGCAGCAACT GTGGGACTGG GACCACCGCA 
     2341 ACCTGACCAT CAAAGCAGGG AAAATGTACT TTGCTTTCAA TCCCAAATTA TGTGTTTCCG 
     2401 AAATTTACCG CATGGAGGAA GTGACGGGGA CTAAAGGGCG CCAAAGCAAA GGGGACATAA 
     2461 ACACCAGGAA CAACGGGGAG AGAGCCTCCT GTGAAAGTGA CGTCCTGCAT TTCACCTCCA 
     2521 CCACCACGTC GAAGAATCGC ATCATCATAA CCTGGCACCG GTACCGGCCC CCTGACTACA 
     2581 GGGATCTCAT CAGCTTCACC GTTTACTACA AGGAAGCACC CTTTAAGAAT GTCACAGAGT 
     2641 ATGATGGGCA GGATGCCTGC GGCTCCAACA GCTGGAACAT GGTGGACGTG GACCTCCCGC 
     2701 CCAACAAGGA CGTGGAGCCC GGCATCTTAC TACATGGGCT GAAGCCCTGG ACTCAGTACG 
     2761 CCGTTTACGT CAAGGCTGTG ACCCTCACCA TGGTGGAGAA CGACCATATC CGTGGGGCCA 
     2821 AGAGTGAGAT CTTGTACATT CGCACCAATG CTTCAGTTCC TTCCATTCCC TTGGACGTTC 
     2881 TTTCAGCATC GAACTCCTCT TCTCAGTTAA TCGTGAAGTG GAACCCTCCC TCTCTGCCCA 
     2941 ACGGCAACCT GAGTTACTAC ATTGTGCGCT GGCAGCGGCA GCCTCAGGAC GGCTACCTTT 
     3001 ACCGGCACAA TTACTGCTCC AAAGACAAAA TCCCCATCAG GAAGTATGCC GACGGCACCA 
     3061 TCGACATTGA GGAGGTCACA GAGAACCCCA AGACTGAGGT GTGTGGTGGG GAGAAAGGGC 
     3121 CTTGCTGCGC CTGCCCCAAA ACTGAAGCCG AGAAGCAGGC CGAGAAGGAG GAGGCTGAAT 
     3181 ACCGCAAAGT CTTTGAGAAT TTCCTGCACA ACTCCATCTT CGTGCCCAGA CCTGAAAGGA 
     3241 AGCGGAGAGA TGTCATGCAA GTGGCCAACA CCACCATGTC CAGCCGAAGC AGGAACACCA 
     3301 CGGCCGCAGA CACCTACAAC ATCACCGACC CGGAAGAGCT GGAGACAGAG TACCCTTTCT 
     3361 TTGAGAGCAG AGTGGATAAC AAGGAGAGAA CTGTCATTTC TAACCTTCGG CCTTTCACAT 
     3421 TGTACCGCAT CGATATCCAC AGCTGCAACC ACGAGGCTGA GAAGCTGGGC TGCAGCGCCT 
     3481 CCAACTTCGT CTTTGCAAGG ACTATGCCCG CAGAAGGAGC AGATGACATT CCTGGGCCAG 
     3541 TGACCTGGGA GCCAAGGCCT GAAAACTCCA TCTTTTTAAA GTGGCCGGAA CCTGAGAATC 
     3601 CCAATGGATT GATTCTAATG TATGAAATAA AATACGGATC ACAAGTTGAG GATCAGCGAG 
     3661 AATGTGTGTC CAGACAGGAA TACAGGAAGT ATGGAGGGGC CAAGCTAAAC CGGCTAAACC 
     3721 CGGGGAACTA CACAGCCCGG ATTCAGGCCA CATCTCTCTC TGGGAATGGG TCGTGGACAG 
     3781 ATCCTGTGTT CTTCTATGTC CAGGCCAAAA CAGGATATGA AAACTTCATC CATCTGATCA 
     3841 TCGCTCTGCC CGTCGCTGTC CTGTTGATCG TGGGAGGGTT GGTGATTATG CTGTACGTCT 
     3901 TCCATAGAAA GAGAAATAAC AGCAGGCTGG GGAATGGAGT GCTGTATGCC TCTGTGAACC 
     3961 CGGAGTACTT CAGCGCTGCT GATGTGTACG TTCCTGATGA GTGGGAGGTG GCTCGGGAGA 
     4021 AGATCACCAT GAGCCGGGAA CTTGGGCAGG GGTCGTTTGG GATGGTCTAT GAAGGAGTTG 
     4081 CCAAGGGTGT GGTGAAAGAT GAACCTGAAA CCAGAGTGGC CATTAAAACA GTGAACGAGG 
     4141 CCGCAAGCAT GCGTGAAAGG ATTGAGTTTC TCAACGAAGC TTCTGTGATG AAGGAGTTCA 
     4201 ATTGTCACCA TGTGGTGCGA TTGCTGGGTG TGGTGTCCCA AGGCCAGCCA ACACTGGTCA 
     4261 TCATGGAACT GATGACACGG GGCGATCTCA AAAGTTATCT CCGGTCTCTG AGGCCAGAAA 
     4321 TGGAGAATAA TCCAGTCCTA GCACCTCCAA GCCTGAGCAA GATGATTCAG ATGGCCGGAG 
     4381 AGATTGCAGA CGGCATGGCA TACCTCAACG CCAATAAGTT CGTCCACAGA GACCTTGCTG 
     4441 CCCGGAATTG CATGGTAGCC GAAGATTTCA CAGTCAAAAT CGGAGATTTT GGTATGACGC 
     4501 GAGATATCTA TGAGACAGAC TATTACCGGA AAGGAGGGAA AGGGCTGCTG CCCGTGCGCT 
     4561 GGATGTCTCC TGAGTCCCTC AAGGATGGAG TCTTCACCAC TTACTCGGAC GTCTGGTCCT 
     4621 TCGGGGTCGT CCTCTGGGAG ATCGCCACAC TGGCCGAGCA GCCCTACCAG GGCTTGTCCA 
     4681 ACGAGCAAGT CCTTCGCTTC GTCATGGAGG GCGGCCTTCT GGACAAGCCA GACAACTGTC 
     4741 CTGACATGCT GTTTGAACTG ATGCGCATGT GCTGGCAGTA TAACCCCAAG ATGAGGCCTT 
     4801 CCTTCCTGGA GATCATCAGC AGCATCAAAG AGGAGATGGA GCCTGGCTTC CGGGAGGTCT 
     4861 CCTTCTACTA CAGCGAGGAG AACAAGCTGC CCGAGCCGGA GGAGCTGGAC CTGGAGCCAG 
     4921 AGAACATGGA GAGCGTCCCC CTGGACCCCT CGGCCTCCTC GTCCTCCCTG CCACTGCCCG 
     4981 ACAGACACTC AGGACACAAG GCCGAGAACG GCCCCGGCCC TGGGGTGCTG GTCCTCCGCG 
     5041 CCAGCTTCGA CGAGAGACAG CCTTACGCCC ACATGAACGG GGGCCGCAAG AACGAGCGGG 
     5101 CCTTGCCGCT GCCCCAGTCT TCGACCTGCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     5161 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     5221 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     5281 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     5341 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5401 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5461 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5521 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5581 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5641 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5701 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5761 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5821 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5881 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5941 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     6001 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     6061 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     6121 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     6181 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     6241 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     6301 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6361 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6421 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6481 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6541 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6601 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6661 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6721 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6781 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6841 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6901 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6961 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     7021 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     7081 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     7141 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     7201 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     7261 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     7321 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7381 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7441 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7501 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7561 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7621 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7681 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7741 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7801 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7861 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7921 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7981 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     8041 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     8101 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     8161 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     8221 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8281 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8341 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8401 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8461 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8521 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8581 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8641 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8701 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8761 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8821 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8881 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8941 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//