LOCUS       RC214926                9324 bp    DNA     circular  SYN 
DEFINITION  PRDM2 (Myc-DDK-tagged)-Human PR domain containing 2, with ZNF domain (PRDM2), transcript variant 3
ACCESSION   NM_001007257
VERSION     NM_001007257.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5481..5572 
                     /label="Myc-DDK"  
     misc_feature    5602..6187 
                     /label="PolyA signal"  
     misc_feature    5659..5678 
                     /label="Restriction Enzyme"  
     misc_feature    6463..7135 
                     /label="ColE1"  
     misc_feature    7565..8359 
                     /label="Kan/Neo"  
     misc_feature    8394..8696 
                     /label="SV40 ori"  
     misc_feature    8782..9259 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5463..5469 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5463 
                     /label="PRDM2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGAGATTCT GCAGAAGGTC CTAAAGAAGA CGAAGAGAAG CCTTCAGCCT 
     1081 CAGCACTTGA GCAGCCGGCC ACCCTCCAGG AGGTGGCCAG TCAGGAGGTG CCTCCAGAAC 
     1141 TAGCAACCCC TGCCCCTGCC TGGGAGCCAC AGCCAGAACC AGACGAGCGA TTAGAAGCGG 
     1201 CAGCTTGTGA GGTGAATGAT TTGGGGGAAG AGGAGGAGGA GGAAGAGGAG GAGGATGAAG 
     1261 AAGAAGAAGA AGATGATGAT GATGATGAGT TGGAAGACGA GGGGGAAGAA GAAGCCAGCA 
     1321 TGCCAAATGA AAATTCTGTG AAAGAGCCAG AAATACGGTG TGATGAGAAG CCAGAAGATT 
     1381 TATTAGAGGA ACCAAAAACA ACTTCAGAAG AAACTCTTGA AGACTGCTCA GAGGTAACAC 
     1441 CTGCCATGCA AATCCCCAGA ACTAAAGAAG AGGCCAATGG TGATGTATTT GAAACGTTTA 
     1501 TGTTTCCGTG TCAACATTGT GAAAGGAAGT TTACAACCAA ACAGGGGCTT GAGCGTCACA 
     1561 TGCATATCCA TATATCCACC GTCAATCATG CTTTCAAATG CAAGTACTGT GGGAAAGCCT 
     1621 TTGGCACACA GATTAACCGG CGGCGACATG AGCGGCGCCA TGAAGCAGGG TTAAAGCGGA 
     1681 AACCCAGCCA AACACTACAG CCGTCAGAGG ATCTGGCTGA TGGCAAAGCA TCTGGAGAAA 
     1741 ACGTTGCTTC AAAAGATGAT TCGAGTCCTC CCAGTCTTGG GCCAGACTGT CTGATCATGA 
     1801 ATTCAGAGAA GGCTTCCCAA GACACAATAA ATTCTTCTGT CGTAGAAGAG AATGGGGAAG 
     1861 TTAAAGAACT TCATCCGTGC AAATATTGTA AAAAGGTTTT TGGAACTCAT ACTAATATGA 
     1921 GACGGCATCA GCGTAGAGTT CACGAACGTC ATCTGATTCC CAAAGGTGTA CGGCGAAAAG 
     1981 GAGGCCTTGA AGAGCCCCAG CCTCCAGCAG AACAGGCCCA GGCCACCCAG AACGTGTATG 
     2041 TACCAAGCAC AGAGCCGGAG GAGGAAGGGG AAGCAGATGA TGTGTACATC ATGGACATTT 
     2101 CTAGCAATAT CTCTGAAAAC TTAAATTACT ATATTGATGG TAAAATTCAA ACTAATAACA 
     2161 ACACTAGTAA CTGTGATGTG ATTGAGATGG AGTCTGCTTC GGCAGATTTG TATGGTATAA 
     2221 ATTGTCTGCT CACTCCAGTT ACAGTGGAAA TTACTCAAAA TATAAAGACC ACACAGGTCC 
     2281 CTGTAACAGA AGATCTTCCT AAAGAGCCTT TGGGCAGCAC AAATAGTGAG GCCAAGAAGC 
     2341 GGAGAACTGC GAGCCCACCT GCACTGCCCA AAATTAAGGC CGAAACAGAC TCTGACCCCA 
     2401 TGGTCCCCTC TTGCTCTTTA AGTCTTCCTC TTAGCATATC AACAACAGAG GCAGTGTCTT 
     2461 TCCACAAAGA GAAAAGTGTT TATTTGTCAT CAAAGCTCAA ACAACTTCTT CAAACCCAAG 
     2521 ATAAACTAAC TCCTGCAGGG ATTTCAGCAA CTGAAATAGC TAAATTAGGT CCTGTTTGTG 
     2581 TGTCTGCTCC TGCATCAATG TTGCCTGTGA CCTCAAGTAG GTTTAAGAGG CGGACCAGCT 
     2641 CTCCTCCCAG TTCTCCACAG CACAGTCCTG CCCTTCGAGA CTTTGGAAAG CCAAGTGATG 
     2701 GGAAAGCAGC ATGGACCGAT GCCGGGCTGA CTTCCAAAAA ATCCAAATTA GAAAGTCACA 
     2761 GCGACTCACC AGCATGGAGT TTGTCTGGGA GAGATGAGAG AGAAACTGTG AGCCCTCCAT 
     2821 GCTTTGATGA ATATAAAATG TCTAAAGAGT GGACAGCTAG TTCTGCTTTT AGCAGTGTGT 
     2881 GCAACCAGCA GCCACTGGAT TTATCCAGCG GTGTCAAACA GAAGGCTGAG GGTACAGGCA 
     2941 AGACTCCAGT CCAGTGGGAA TCTGTCTTAG ATCTCAGTGT GCATAAAAAG CATTGTAGTG 
     3001 ACTCTGAAGG CAAGGAATTC AAAGAAAGTC ATTCAGTGCA GCCTACGTGT AGTGCTGTAA 
     3061 AGAAAAGGAA ACCAACCACC TGCATGCTGC AGAAGGTTCT TCTCAATGAA TATAATGGCA 
     3121 TCGATTTACC TGTAGAAAAC CCTGCAGATG GGACCAGGAG CCCAAGTCCT TGTAAATCCC 
     3181 TAGAAGCTCA GCCAGATCCT GACCTCGGTC CGGGCTCTGG TTTCCCTGCC CCTACTGTTG 
     3241 AGTCCACACC TGATGTTTGT CCTTCATCAC CTGCCCTGCA GACACCCTCC CTTTCATCCG 
     3301 GTCAGCTGCC TCCTCTCTTG ATCCCCACAG ATCCCTCTTC CCCTCCACCC TGTCCCCCGG 
     3361 TATTAACTGT TGCCACTCCG CCCCCTCCCC TCCTTCCTAC CGTACCTCTT CCAGCCCCCT 
     3421 CTTCCAGTGC ATCTCCACAC CCATGCCCCT CTCCACTCTC AAATGCCACC GCACAGTCCC 
     3481 CACTTCCAAT TCTGTCCCCA ACAGTGTCCC CCTCTCCCTC TCCCATTCCT CCCGTGGAGC 
     3541 CCCTGATGTC TGCCGCCTCA CCCGGGCCTC CAACACTTTC TTCTTCCTCC TCTTCATCTT 
     3601 CCTCCTCCTC TTCGTTTTCT TCTTCATCTT CCTCCTCTTC TCCTTCTCCA CCTCCTCTCT 
     3661 CCGCAATATC ATCTGTTGTT TCCTCTGGTG ATAATCTGGA GGCTTCTCTC CCCATGATAT 
     3721 CTTTCAAACA GGAGGAATTA GAGAATGAAG GTCTGAAACC CAGGGAAGAG CCCCAGTCTG 
     3781 CTGCTGAACA GGATGTTGTT GTTCAGGAAA CATTCAACAA AAACTTTGTT TGCAACGTCT 
     3841 GTGAATCACC TTTTCTTTCC ATTAAAGATC TAACCAAACA TTTATCTATT CATGCTGAAG 
     3901 AATGGCCCTT CAAATGTGAA TTTTGTGTGC AGCTTTTTAA GGATAAAACG GACTTGTCAG 
     3961 AACATCGCTT TTTGCTTCAT GGAGTTGGGA ATATCTTTGT GTGTTCTGTT TGTAAAAAAG 
     4021 AATTTGCTTT TTTGTGCAAT TTGCAGCAGC ACCAGCGAGA TCTCCACCCA GATAAGGTGT 
     4081 GCACACATCA CGAGTTTGAA AGCGGGACTC TGAGGCCCCA GAACTTTACA GATCCCAGCA 
     4141 AGGCCCATGT AGAGCATATG CAGAGCTTGC CAGAAGATCC TTTAGAAACT TCTAAAGAAG 
     4201 AAGAGGAGTT AAATGATTCC TCTGAAGAGC TTTACACGAC TATAAAAATA ATGGCTTCTG 
     4261 GAATAAAGAC AAAAGATCCA GATGTTCGAT TGGGCCTCAA TCAGCATTAC CCAAGCTTTA 
     4321 AACCACCTCC ATTTCAGTAC CATCACCGTA ACCCCATGGG GATTGGTGTG ACAGCCACAA 
     4381 ATTTCACTAC ACACAATATT CCACAGACTT TCACTACCGC CATTCGCTGC ACAAAGTGTG 
     4441 GAAAAGGTGT CGACAATATG CCGGAGTTGC ACAAACATAT CCTGGCTTGT GCTTCTGCAA 
     4501 GTGACAAGAA GAGGTACACG CCTAAGAAAA ACCCAGTACC ATTAAAACAA ACTGTGCAAC 
     4561 CCAAAAATGG CGTGGTGGTT TTAGATAACT CTGGGAAAAA TGCCTTCCGA CGAATGGGAC 
     4621 AGCCCAAAAG GCTTAACTTT AGTGTTGAGC TCAGCAAAAT GTCGTCGAAT AAGCTCAAAT 
     4681 TAAATGCATT GAAGAAAAAA AATCAGCTAG TACAGAAAGC AATTCTTCAG AAAAACAAAT 
     4741 CTGCAAAGCA GAAGGCCGAC TTGAAAAATG CTTGTGAGTC ATCCTCTCAC ATCTGCCCTT 
     4801 ACTGTAATCG AGAGTTCACT TACATTGGAA GCCTGAATAA ACACGCCGCC TTCAGCTGTC 
     4861 CCAAAAAACC CCTTTCTCCT CCCAAAAAAA AAGTTTCTCA TTCATCTAAG AAAGGTGGAC 
     4921 ACTCATCACC TGCAAGTAGT GACAAAAACA GTAACAGCAA CCACCGCAGA CGGACAGCGG 
     4981 ATGCGGAGAT TAAAATGCAA AGCATGCAGA CTCCGTTGGG CAAGACCAGA GCCCGCAGCT 
     5041 CAGGCCCCAC CCAAGTCCCA CTTCCCTCCT CATCCTTCAG GTCCAAGCAG AACGTCAAGT 
     5101 TTGCAGCTTC GGTGAAATCC AAAAAACCAA GCTCCTCCTC TTTAAGGAAC TCCAGCCCGA 
     5161 TAAGAATGGC CAAAATAACT CATGTTGAGG GGAAAAAACC TAAAGCTGTG GCCAAGAATC 
     5221 ATTCTGCTCA GCTTTCCAGC AAAACATCAC GGAGCCTGCA CGTGAGGGTA CAGAAAAGCA 
     5281 AAGCTGTTTT ACAAAGCAAA TCCACCTTGG CGAGTAAGAA AAGAACAGAC CGGTTCAATA 
     5341 TAAAATCTAG AGAGCGGAGT GGGGGGCCAG TCACCCGGAG CCTTCAGCTG GCAGCTGCTG 
     5401 CTGACTTGAG TGAGAACAAG AGAGAGGACG GCAGCGCCAA GCAGGAGCTG AAGGACTTCA 
     5461 GGAACTTCCT GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5521 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5581 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5641 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5701 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5761 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5821 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5881 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5941 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     6001 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     6061 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     6121 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     6181 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     6241 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     6301 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     6361 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     6421 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6481 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6541 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6601 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6661 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6721 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6781 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6841 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6901 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6961 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     7021 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     7081 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     7141 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     7201 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     7261 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     7321 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     7381 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     7441 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7501 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7561 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7621 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7681 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7741 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7801 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7861 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7921 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7981 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     8041 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     8101 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     8161 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     8221 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     8281 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     8341 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     8401 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     8461 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8521 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8581 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8641 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8701 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8761 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8821 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8881 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8941 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     9001 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     9061 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     9121 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     9181 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     9241 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     9301 TAACAAAAAT TTAACGCGAA TTTT
//