LOCUS       RC214918                9513 bp    DNA     circular  SYN 
DEFINITION  KDM5B (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 5B (KDM5B)
ACCESSION   NM_006618
VERSION     NM_006618.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5670..5761 
                     /label="Myc-DDK"  
     misc_feature    5791..6376 
                     /label="PolyA signal"  
     misc_feature    5848..5867 
                     /label="Restriction Enzyme"  
     misc_feature    6652..7324 
                     /label="ColE1"  
     misc_feature    7754..8548 
                     /label="Kan/Neo"  
     misc_feature    8583..8885 
                     /label="SV40 ori"  
     misc_feature    8971..9448 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5652..5658 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5652 
                     /label="KDM5B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCGGCC ACCACACTGC ACCCAGGCCC GCGCCCGGCG CTGCCCCTCG 
     1081 GGGGCCCGGG CCCGCTGGGC GAGTTCCTGC CTCCACCCGA GTGCCCGGTC TTCGAACCCA 
     1141 GCTGGGAAGA GTTCGCGGAC CCCTTCGCTT TCATCCACAA GATCCGGCCC ATAGCCGAGC 
     1201 AGACTGGCAT CTGTAAGGTG CGGCCGCCGC CGGATTGGCA GCCACCATTT GCATGTGATG 
     1261 TTGATAAACT TCATTTTACG CCACGTATCC AGAGACTGAA TGAATTGGAG GCCCAAACTC 
     1321 GTGTAAAATT GAATTTCTTG GACCAGATTG CAAAGTACTG GGAGTTACAG GGAAGTACTC 
     1381 TGAAAATTCC ACATGTGGAG AGGAAGATCT TGGACTTATT TCAGCTTAAT AAGTTAGTTG 
     1441 CAGAAGAAGG TGGATTTGCA GTTGTTTGCA AGGATAGAAA ATGGACCAAA ATTGCTACCA 
     1501 AGATGGGGTT TGCTCCTGGC AAAGCAGTGG GCTCACATAT CAGAGGGCAT TATGAACGAA 
     1561 TTCTCAACCC CTACAACTTA TTCCTGTCCG GAGACAGCCT AAGGTGTTTG CAGAAGCCAA 
     1621 ACCTGACCAC AGACACTAAG GACAAGGAGT ACAAACCCCA TGATATTCCC CAGAGGCAGT 
     1681 CTGTGCAGCC TTCGGAAACG TGCCCCCCAG CCCGACGAGC AAAACGCATG AGAGCAGAGG 
     1741 CCATGAATAT TAAAATAGAA CCCGAGGAGA CAACAGAAGC CAGAACTCAT AATCTGAGAC 
     1801 GTCGAATGGG TTGTCCAACT CCAAAATGTG AAAATGAGAA AGAAATGAAG AGTAGCATCA 
     1861 AGCAAGAACC TATTGAGAGG AAAGATTATA TTGTAGAAAA TGAGAAGGAA AAGCCCAAGA 
     1921 GTCGATCTAA AAAAGCCACC AATGCTGTGG ACCTGTATGT CTGTCTTTTA TGTGGCAGTG 
     1981 GCAATGATGA AGACCGGCTA CTGTTGTGTG ATGGCTGTGA TGACAGTTAC CATACCTTTT 
     2041 GCTTGATCCC ACCTCTCCAT GATGTTCCCA AGGGAGACTG GAGGTGTCCT AAGTGTTTGG 
     2101 CTCAGGAATG TAGTAAGCCA CAAGAAGCAT TTGGCTTTGA ACAAGCAGCC AGGGACTATA 
     2161 CCCTCCGTAC TTTTGGGGAA ATGGCAGATG CGTTCAAATC TGATTACTTC AACATGCCAG 
     2221 TCCATATGGT CCCCACAGAG CTTGTTGAGA AAGAATTTTG GAGACTAGTA AGCACTATTG 
     2281 AGGAGGATGT CACAGTGGAA TATGGAGCTG ACATTGCCTC AAAGGAATTT GGCAGTGGCT 
     2341 TTCCTGTCCG AGATGGGAAA ATCAAACTCT CACCTGAGGA AGAGGAGTAT CTTGATAGTG 
     2401 GCTGGAATTT GAACAACATG CCAGTGATGG AGCAGTCTGT CCTTGCACAT ATTACTGCTG 
     2461 ATATATGTGG CATGAAACTT CCTTGGTTGT ATGTGGGAAT GTGCTTTTCT TCATTCTGTT 
     2521 GGCACATTGA AGACCACTGG AGCTATTCAA TTAACTACTT GCACTGGGGT GAGCCAAAAA 
     2581 CCTGGTATGG AGTCCCAGGG TATGCTGCTG AGCAGCTAGA AAATGTAATG AAGAAACTAG 
     2641 CTCCAGAACT CTTTGTGTCC CAGCCGGATC TCCTCCATCA GCTTGTGACC ATCATGAACC 
     2701 CCAATACCCT GATGACTCAT GAAGTGCCTG TTTACCGAAC TAATCAGTGT GCTGGGGAGT 
     2761 TTGTGATTAC ATTTCCAAGA GCCTACCACA GTGGTTTTAA CCAGGGTTTT AATTTTGCTG 
     2821 AGGCTGTTAA CTTCTGCACT GTTGATTGGC TGCCATTAGG CCGACAGTGT GTGGAGCATT 
     2881 ATCGCTTGCT TCATCGATAT TGTGTGTTTT CCCATGATGA GATGATCTGC AAGATGGCTT 
     2941 CCAAGGCTGA TGTATTAGAT GTTGTAGTGG CTTCAACTGT TCAGAAAGAC ATGGCCATTA 
     3001 TGATTGAGGA TGAGAAAGCT TTAAGAGAAA CTGTCCGTAA ATTGGGAGTG ATTGATTCGG 
     3061 AAAGAATGGA TTTTGAGCTG TTGCCAGATG ATGAACGTCA GTGTGTAAAA TGCAAAACTA 
     3121 CATGCTTCAT GTCTGCCATC TCCTGTTCTT GTAAACCTGG CCTTCTTGTT TGCCTGCATC 
     3181 ATGTAAAAGA ATTGTGTTCC TGTCCTCCTT ATAAATATAA ATTGCGGTAT AGGTACACGC 
     3241 TGGATGATCT CTACCCTATG ATGAATGCAT TGAAGCTTCG AGCAGAATCT TACAACGAAT 
     3301 GGGCCTTGAA TGTGAATGAA GCTTTGGAGG CAAAGATCAA CAAGAAGAAA AGCCTTGTCA 
     3361 GCTTCAAGGC TTTAATTGAA GAATCTGAAA TGAAGAAATT CCCAGACAAT GATCTTTTGC 
     3421 GACACCTTCG CCTAGTCACA CAGGATGCAG AGAAGTGTGC CTCTGTTGCG CAGCAGTTGC 
     3481 TTAATGGCAA AAGGCAAACT AGATATCGAT CTGGTGGAGG GAAATCCCAA AATCAGTTGA 
     3541 CAGTGAATGA GCTCCGGCAG TTTGTAACAC AGCTGTATGC TCTTCCATGT GTCCTCAGTC 
     3601 AGACACCATT ACTAAAGGAT CTCTTGAATC GTGTAGAAGA TTTTCAACAG CATAGTCAGA 
     3661 AACTACTCTC TGAGGAAACG CCTAGTGCTG CGGAGCTGCA GGACTTGCTA GATGTCAGCT 
     3721 TTGAATTTGA TGTTGAACTT CCACAGCTTG CTGAGATGCG TATCCGTTTG GAACAAGCCC 
     3781 GTTGGCTAGA AGAGGTGCAG CAAGCTTGCC TAGACCCCAG CTCCCTTACT TTAGATGATA 
     3841 TGAGACGTCT CATAGACCTA GGGGTAGGGC TGGCCCCGTA TTCAGCAGTG GAGAAAGCTA 
     3901 TGGCCCGGCT GCAGGAACTG CTCACAGTGT CAGAGCACTG GGACGACAAA GCCAAGAGTC 
     3961 TCCTCAAGGC CAGGCCACGA CATTCATTGA ATAGCCTTGC TACGGCAGTA AAGGAAATCG 
     4021 AAGAGATCCC TGCATATCTG CCCAATGGTG CGGCTCTGAA AGACTCAGTG CAGAGAGCCA 
     4081 GAGACTGGCT TCAGGATGTA GAGGGCCTGC AGGCTGGAGG ACGTGTGCCA GTGTTAGACA 
     4141 CACTCATAGA ACTTGTTACA CGAGGCCGAT CTATCCCGGT ACATCTGAAT TCTTTGCCAA 
     4201 GACTGGAAAC CCTAGTAGCT GAGGTTCAGG CTTGGAAAGA ATGTGCTGTT AATACATTCT 
     4261 TGACTGAGAA TTCTCCATAT TCTCTCTTAG AGGTGCTGTG TCCTCGATGT GATATTGGCC 
     4321 TTTTGGGATT GAAAAGGAAG CAGAGAAAGT TAAAGGAGCC CTTGCCAAAT GGAAAGAAAA 
     4381 AAAGCACCAA ATTAGAGAGT CTGAGTGACC TGGAGAGAGC TTTAACTGAA AGCAAGGAGA 
     4441 CTGCTTCAGC TATGGCAACT CTTGGGGAAG CTCGCCTAAG GGAAATGGAA GCCTTGCAGT 
     4501 CTCTCAGACT CGCCAATGAA GGGAAATTGC TGTCGCCTCT CCAAGATGTG GATATAAAAA 
     4561 TCTGCCTATG TCAGAAGGCC CCAGCTGCCC CTATGATTCA ATGTGAACTC TGCAGGGATG 
     4621 CTTTCCACAC CAGTTGTGTG GCGGTACCCA GTATTTCACA GGGCCTGCGA ATCTGGCTTT 
     4681 GTCCCCATTG TCGGAGGTCA GAGAAACCTC CATTAGAGAA AATTCTGCCC CTGCTCGCCT 
     4741 CCCTTCAGCG TATCCGAGTT CGCCTTCCTG AGGGAGATGC ACTTCGATAT ATGATTGAAA 
     4801 GAACCGTGAA CTGGCAGCAC AGAGCCCAGC AACTGCTTTC GTCAGGGAAT CTTAAATTTG 
     4861 TGCAAGATCG AGTGGGCTCA GGACTGTTAT ATAGCAGATG GCAAGCCTCA GCAGGACAGG 
     4921 TGTCAGACAC AAACAAGGTA TCTCAACCTC CTGGCACAAC ATCATTTTCT TTGCCTGATG 
     4981 ACTGGGACAA CAGAACCTCA TATTTGCACT CCCCCTTCTC AACTGGACGA AGTTGTATCC 
     5041 CCCTCCATGG TGTTAGTCCA GAAGTGAATG AACTATTGAT GGAAGCCCAG CTGCTCCAGG 
     5101 TATCCCTTCC TGAAATTCAG GAACTTTACC AGACTTTACT TGCAAAGCCA AGCCCTGCTC 
     5161 AGCAGACTGA CCGAAGCTCA CCAGTGAGAC CCAGCAGTGA GAAGAATGAC TGTTGCCGAG 
     5221 GGAAGCGAGA TGGAATTAAC AGTCTTGAGA GAAAACTGAA GAGACGCCTG GAAAGAGAGG 
     5281 GCCTCTCCAG TGAGCGGTGG GAACGAGTTA AGAAAATGCG GACCCCCAAA AAGAAGAAAA 
     5341 TCAAACTGAG CCACCCCAAG GACATGAACA ATTTCAAGTT AGAGAGAGAG CGTAGCTATG 
     5401 AATTAGTTCG TTCTGCTGAA ACTCATTCCC TGCCCTCAGA CACATCCTAT TCCGAACAGG 
     5461 AAGACTCTGA GGATGAAGAT GCCATCTGCC CAGCTGTGAG CTGCCTGCAG CCAGAAGGAG 
     5521 ATGAGGTGGA CTGGGTCCAG TGTGATGGCA GCTGCAATCA GTGGTTTCAT CAGGTCTGTG 
     5581 TTGGTGTCTC CCCAGAGATG GCAGAGAAAG AAGACTACAT CTGTGTGCGC TGTACTGTGA 
     5641 AGGACGCACC AAGCCGAAAG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5701 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5761 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5821 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5881 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5941 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     6001 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     6061 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     6121 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     6181 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     6241 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     6301 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     6361 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     6421 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     6481 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     6541 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     6601 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6661 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6721 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6781 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6841 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6901 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6961 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     7021 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     7081 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     7141 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     7201 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     7261 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     7321 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     7381 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     7441 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     7501 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     7561 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     7621 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7681 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7741 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7801 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7861 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7921 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7981 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     8041 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     8101 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     8161 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     8221 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     8281 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     8341 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     8401 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     8461 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     8521 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     8581 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     8641 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8701 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8761 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8821 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8881 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8941 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     9001 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     9061 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     9121 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     9181 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     9241 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     9301 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     9361 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     9421 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     9481 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//