LOCUS       RC214871                7227 bp    DNA     circular  SYN 
DEFINITION  GSN (Myc-DDK-tagged)-Human gelsolin (GSN), transcript variant 1
ACCESSION   NM_000177
VERSION     NM_000177.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3384..3475 
                     /label="Myc-DDK"  
     misc_feature    3505..4090 
                     /label="PolyA signal"  
     misc_feature    3562..3581 
                     /label="Restriction Enzyme"  
     misc_feature    4366..5038 
                     /label="ColE1"  
     misc_feature    5468..6262 
                     /label="Kan/Neo"  
     misc_feature    6297..6599 
                     /label="SV40 ori"  
     misc_feature    6685..7162 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3366..3372 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3366 
                     /label="GSN"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCCGCAC CGCCCCGCGC CCGCGCTGCT TTGCGCGCTG TCCCTGGCGC 
     1081 TGTGCGCGCT GTCGCTGCCC GTCCGCGCGG CCACTGCGTC GCGGGGGGCG TCCCAGGCGG 
     1141 GGGCGCCCCA GGGGCGGGTG CCCGAGGCGC GGCCCAACAG CATGGTGGTG GAACACCCCG 
     1201 AGTTCCTCAA GGCAGGGAAG GAGCCTGGCC TGCAGATCTG GCGTGTGGAG AAGTTCGATC 
     1261 TGGTGCCCGT GCCCACCAAC CTTTATGGAG ACTTCTTCAC GGGCGACGCC TACGTCATCC 
     1321 TGAAGACAGT GCAGCTGAGG AACGGAAATC TGCAGTATGA CCTCCACTAC TGGCTGGGCA 
     1381 ATGAGTGCAG CCAGGATGAG AGCGGGGCGG CCGCCATCTT TACCGTGCAG CTGGATGACT 
     1441 ACCTGAACGG CCGGGCCGTG CAGCACCGTG AGGTCCAGGG CTTCGAGTCG GCCACCTTCC 
     1501 TAGGCTACTT CAAGTCTGGC CTGAAGTACA AGAAAGGAGG TGTGGCATCA GGATTCAAGC 
     1561 ACGTGGTACC CAACGAGGTG GTGGTGCAGA GACTCTTCCA GGTCAAAGGG CGGCGTGTGG 
     1621 TCCGTGCCAC CGAGGTACCT GTGTCCTGGG AGAGCTTCAA CAATGGCGAC TGCTTCATCC 
     1681 TGGACCTGGG CAACAACATC CACCAGTGGT GTGGTTCCAA CAGCAATCGG TATGAAAGAC 
     1741 TGAAGGCCAC ACAGGTGTCC AAGGGCATCC GGGACAACGA GCGGAGTGGC CGGGCCCGAG 
     1801 TGCACGTGTC TGAGGAGGGC ACTGAGCCCG AGGCGATGCT CCAGGTGCTG GGCCCCAAGC 
     1861 CGGCTCTGCC TGCAGGTACC GAGGACACCG CCAAGGAGGA TGCGGCCAAC CGCAAGCTGG 
     1921 CCAAGCTCTA CAAGGTCTCC AATGGTGCAG GGACCATGTC CGTCTCCCTC GTGGCTGATG 
     1981 AGAACCCCTT CGCCCAGGGG GCCCTGAAGT CAGAGGACTG CTTCATCCTG GACCACGGCA 
     2041 AAGATGGGAA AATCTTTGTC TGGAAAGGCA AGCAGGCAAA CACGGAGGAG AGGAAGGCTG 
     2101 CCCTCAAAAC AGCCTCTGAC TTCATCACCA AGATGGACTA CCCCAAGCAG ACTCAGGTCT 
     2161 CGGTCCTTCC TGAGGGCGGT GAGACCCCAC TGTTCAAGCA GTTCTTCAAG AACTGGCGGG 
     2221 ACCCAGACCA GACAGATGGC CTGGGCTTGT CCTACCTTTC CAGCCATATC GCCAACGTGG 
     2281 AGCGGGTGCC CTTCGACGCC GCCACCCTGC ACACCTCCAC TGCCATGGCC GCCCAGCACG 
     2341 GCATGGATGA CGATGGCACA GGCCAGAAAC AGATCTGGAG AATCGAAGGT TCCAACAAGG 
     2401 TGCCCGTGGA CCCTGCCACA TATGGACAGT TCTATGGAGG CGACAGCTAC ATCATTCTGT 
     2461 ACAACTACCG CCATGGTGGC CGCCAGGGGC AGATAATCTA TAACTGGCAG GGTGCCCAGT 
     2521 CTACCCAGGA TGAGGTCGCT GCATCTGCCA TCCTGACTGC TCAGCTGGAT GAGGAGCTGG 
     2581 GAGGTACCCC TGTCCAGAGC CGTGTGGTCC AAGGCAAGGA GCCCGCCCAC CTCATGAGCC 
     2641 TGTTTGGTGG GAAGCCCATG ATCATCTACA AGGGCGGCAC CTCCCGCGAG GGCGGGCAGA 
     2701 CAGCCCCTGC CAGCACCCGC CTCTTCCAGG TCCGCGCCAA CAGCGCTGGA GCCACCCGGG 
     2761 CTGTTGAGGT ATTGCCTAAG GCTGGTGCAC TGAACTCCAA CGATGCCTTT GTTCTGAAAA 
     2821 CCCCCTCAGC CGCCTACCTG TGGGTGGGTA CAGGAGCCAG CGAGGCAGAG AAGACGGGGG 
     2881 CCCAGGAGCT GCTCAGGGTG CTGCGGGCCC AACCTGTGCA GGTGGCAGAA GGCAGCGAGC 
     2941 CAGATGGCTT CTGGGAGGCC CTGGGCGGGA AGGCTGCCTA CCGCACATCC CCACGGCTGA 
     3001 AGGACAAGAA GATGGATGCC CATCCTCCTC GCCTCTTTGC CTGCTCCAAC AAGATTGGAC 
     3061 GTTTTGTGAT CGAAGAGGTT CCTGGTGAGC TCATGCAGGA AGACCTGGCA ACGGATGACG 
     3121 TCATGCTTCT GGACACCTGG GACCAGGTCT TTGTCTGGGT TGGAAAGGAT TCTCAAGAAG 
     3181 AAGAAAAGAC AGAAGCCTTG ACTTCTGCTA AGCGGTACAT CGAGACGGAC CCAGCCAATC 
     3241 GGGATCGGCG GACGCCCATC ACCGTGGTGA AGCAAGGCTT TGAGCCTCCC TCCTTTGTGG 
     3301 GCTGGTTCCT TGGCTGGGAT GATGATTACT GGTCTGTGGA CCCCTTGGAC AGGGCCATGG 
     3361 CTGAGCTGGC TGCCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     3421 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     3481 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     3541 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     3601 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     3661 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     3721 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     3781 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     3841 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     3901 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     3961 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4021 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4081 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4141 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4201 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     4261 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     4321 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     4381 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     4441 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4501 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     4561 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     4621 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     4681 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     4741 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     4801 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     4861 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     4921 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     4981 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5041 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5101 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5161 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     5221 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     5281 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     5341 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     5401 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     5461 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     5521 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     5581 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     5641 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     5701 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     5761 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     5821 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     5881 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     5941 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6001 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6061 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6121 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6181 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     6241 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     6301 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     6361 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     6421 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     6481 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6541 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6601 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     6661 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     6721 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     6781 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     6841 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     6901 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     6961 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7021 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7081 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7141 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7201 ATTTAACAAA AATTTAACGC GAATTTT
//