LOCUS       RC214820                8649 bp    DNA     circular  SYN 
DEFINITION  KCNT1 (Myc-DDK-tagged)-Human potassium channel, subfamily T, member 1 (KCNT1)
ACCESSION   NM_020822
VERSION     NM_020822.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4806..4897 
                     /label="Myc-DDK"  
     misc_feature    4927..5512 
                     /label="PolyA signal"  
     misc_feature    4984..5003 
                     /label="Restriction Enzyme"  
     misc_feature    5788..6460 
                     /label="ColE1"  
     misc_feature    6890..7684 
                     /label="Kan/Neo"  
     misc_feature    7719..8021 
                     /label="SV40 ori"  
     misc_feature    8107..8584 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4788..4794 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4788 
                     /label="KCNT1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCACTCCCT GACGGGGCGC GGACCCCGGG GGGCGTCTGC CGGGAGGCGC 
     1081 GCGGCGGGGG CTACACCAAC CGGACCTTCG AGTTTGACGA CGGCCAATGC GCCCCCAGGC 
     1141 GGCCCTGCGC GGGGGACGGC GCGCTCCTGG ACACCGCCGG CTTCAAGATG AGCGACCTGG 
     1201 ACTCCGAGGT GCTGCCCTTG CCGCCGCGCT ACCGCTTCCG GGACCTGCTG CTGGGCGACC 
     1261 CGTCCTTCCA GAACGACGAC AGGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA 
     1321 AGGAGCGGCT CAAGCTGTTC TTCATCAAAA ACCAAAGATC GAGCCTGAGG ATCCGGCTGT 
     1381 TCAACTTCTC CCTGAAGCTG CTCACCTGCC TGCTCTACAT TGTGCGCGTC CTGCTCGATG 
     1441 ACCCGGCCCT GGGCATCGGA TGCTGGGGCT GCCCAAAGCA GAACTACTCC TTCAATGACT 
     1501 CGTCCTCCGA GATCAACTGG GCTCCTATTC TGTGGGTGGA GAGAAAGATG ACACTGTGGG 
     1561 CGATCCAGGT CATCGTGGCC ATAATAAGCT TCCTGGAGAC GATGCTTCTC ATCTACCTCA 
     1621 GCTACAAAGG CAACATCTGG GAGCAGATCT TCCGCGTGTC CTTCGTCCTG GAGATGATCA 
     1681 ACACTCTGCC CTTCATCATC ACGATCTTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG 
     1741 TCTTTCTGAA CTGCTGGCTG GCCAAGCACG CGCTGGAAAA CATGATTAAT GACTTCCACC 
     1801 GTGCCATCCT GCGGACACAG TCAGCCATGT TCAACCAGGT CCTCATCCTC TTCTGCACCC 
     1861 TGCTGTGCCT CGTTTTCACG GGGACCTGCG GCATCCAGCA CCTGGAGCGG GCGGGCGAGA 
     1921 ACCTGTCCCT CCTGACCTCC TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTACG 
     1981 GTGACGTCAC GCCCAAGATC TGGCCATCGC AGCTGCTGGT GGTCATCATG ATCTGCGTGG 
     2041 CCCTCGTGGT GCTCCCACTG CAGTTCGAGG AGCTCGTCTA CCTCTGGATG GAGCGGCAGA 
     2101 AGTCAGGGGG CAACTACAGC CGCCACCGTG CGCAGACGGA GAAGCACGTG GTCCTGTGTG 
     2161 TCAGCTCCCT CAAGATCGAC CTTCTCATGG ACTTCCTGAA CGAGTTCTAC GCCCACCCCC 
     2221 GGCTCCAGGA CTATTACGTG GTCATCCTGT GCCCCACGGA GATGGATGTC CAGGTGCGCA 
     2281 GAGTCCTGCA GATCCCTCTG TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCTGCACTCA 
     2341 AAGACCAGGA CCTCATGCGA GCCAAGATGG ACAATGGGGA GGCCTGCTTC ATCCTCAGCA 
     2401 GCAGGAACGA GGTGGACCGC ACGGCTGCAG ACCACCAGAC CATCCTGCGC GCCTGGGCCG 
     2461 TGAAGGACTT CGCCCCCAAC TGCCCCCTCT ACGTCCAGAT CCTCAAACCT GAAAACAAGT 
     2521 TTCACGTCAA GTTTGCTGAC CACGTGGTGT GTGAGGAGGA GTGCAAGTAC GCCATGCTGG 
     2581 CGCTGAACTG CATCTGCCCG GCGACCTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC 
     2641 GCGGCCAGGA GGGACAGGAG TCTCCGGAGC AGTGGCAGCG CATGTATGGG CGCTGCTCCG 
     2701 GCAACGAGGT GTACCACATC CGCATGGGTG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA 
     2761 AGAGCTTCAC CTACGCGGCC TTCCACGCCC ACAAGAAGTA TGGCGTGTGC CTCATCGGGC 
     2821 TGAAGCGGGA GGACAACAAG AGCATCCTGC TGAACCCGGG GCCCCGGCAC ATCCTGGCCG 
     2881 CCTCTGACAC CTGCTTCTAC ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA 
     2941 AGCAGGAGGA GAAGCGGAAG AAGAGGGCCT TCTCGGGGCA GGGGCTGCAC GAGGGTCCGG 
     3001 CCCGCCTGCC CGTGCACAGC ATCATCGCCT CCATGGGGAC AGTGGCCATG GACCTGCAGG 
     3061 GCACAGAGCA CCGGCCTACG CAGAGCGGCG GTGGGGGCGG GGGCAGCAAG CTGGCACTGC 
     3121 CCACGGAGAA CGGCTCGGGC AGCCGGCGGC CCAGCATCGC GCCCGTCCTG GAACTGGCCG 
     3181 ACAGCTCAGC CCTGCTGCCC TGCGACCTGC TGAGCGACCA GTCGGAGGAT GAGGTGACGC 
     3241 CGTCGGACGA CGAGGGGCTC TCCGTGGTAG AGTATGTGAA GGGCTACCCT CCCAACTCGC 
     3301 CCTACATCGG CAGCTCCCCA ACCCTGTGCC ACCTCCTGCC TGTGAAAGCC CCCTTCTGCT 
     3361 GCCTGCGGCT GGACAAGGGC TGCAAGCACA ACAGCTATGA AGACGCCAAG GCCTACGGGT 
     3421 TCAAGAACAA GCTGATCATC GTCTCGGCAG AGACGGCCGG CAATGGGCTG TACAACTTCA 
     3481 TCGTGCCACT GCGGGCCTAC TACAGATCCC GCAAGGAGCT GAACCCCATC GTGCTGCTGC 
     3541 TGGACAACAA GCCCGACCAC CACTTCCTGG AAGCCATCTG CTGCTTCCCC ATGGTCTACT 
     3601 ACATGGAGGG CTCTGTGGAC AACCTGGACA GCCTGCTGCA GTGTGGCATC ATCTATGCGG 
     3661 ACAACCTGGT GGTGGTGGAC AAGGAGAGCA CCATGAGCGC CGAGGAGGAC TACATGGCGG 
     3721 ACGCCAAGAC CATCGTCAAC GTGCAGACCA TGTTCCGGCT CTTCCCCAGC CTCAGCATCA 
     3781 CCACGGAGCT CACCCACCCT TCCAACATGC GCTTCATGCA GTTCCGCGCC AAGGACAGCT 
     3841 ACTCTCTGGC TCTTTCCAAA CTAGAAAAGA GGGAGCGAGA GAATGGCTCC AACCTGGCCT 
     3901 TCATGTTCCG CCTGCCGTTC GCCGCCGGCC GCGTCTTCAG CATCAGCATG TTGGACACAC 
     3961 TGCTCTACCA GTCCTTCGTG AAGGACTACA TGATCACCAT CACCCGGCTG CTGCTGGGCC 
     4021 TGGACACCAC GCCGGGCTCG GGGTACCTCT GTGCCATGAA AATCACCGAG GGCGACCTGT 
     4081 GGATCCGCAC GTACGGCCGC CTCTTCCAGA AGCTCTGCTC CTCCAGCGCC GAGATCCCCA 
     4141 TTGGCATCTA CCGGACAGAG AGCCACGTCT TCTCCACCTC GGAGCCCCAC GACCTCAGAG 
     4201 CCCAGTCCCA GATCTCGGTG AACGTGGAGG ACTGTGAGGA CACACGGGAA GTGAAGGGGC 
     4261 CCTGGGGCTC CCGCGCTGGC ACCGGAGGCA GCTCCCAGGG CCGCCACACG GGCGGCGGTG 
     4321 ACCCCGCAGA GCACCCACTG CTACGGCGCA AGAGCCTGCA GTGGGCCCGG AGGCTGAGCC 
     4381 GCAAGGCGCC CAAGCAGGCA GGCCGGGCGG CGGCCGCGGA GTGGATCAGC CAGCAGCGCC 
     4441 TCAGCCTGTA CCGGCGCTCT GAGCGCCAGG AGCTCTCCGA GCTGGTGAAG AACCGCATGA 
     4501 AGCACCTGGG GCTGCCCACC ACCGGCTACG AGGACGTAGC AAATTTAACA GCCAGTGATG 
     4561 TCATGAATCG GGTAAACCTG GGATATTTGC AAGACGAGAT GAACGACCAC CAGAACACCC 
     4621 TCTCCTACGT CCTCATCAAC CCTCCGCCCG ACACGAGGCT GGAGCCCAGT GACATTGTCT 
     4681 ATCTCATCCG CTCCGACCCC CTGGCTCACG TGGCCAGCAG CTCCCAGAGC CGGAAGAGCA 
     4741 GCTGCAGCCA CAAGCTGTCG TCCTGCAACC CCGAGACTCG CGACGAGACA CAGCTCACGC 
     4801 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4861 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4921 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4981 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5041 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5101 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5161 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5221 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5281 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5341 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5401 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5461 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5521 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5581 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5641 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5701 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5761 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5821 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5881 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5941 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6001 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6061 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6121 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6181 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6241 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6301 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6361 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6421 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6481 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6541 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6601 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6661 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6721 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6781 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6841 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6901 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6961 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7021 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7081 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7141 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7201 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7261 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7321 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7381 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7441 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7501 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7561 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7621 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7681 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7741 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7801 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7861 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7921 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7981 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8041 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8101 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8161 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8221 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8281 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8341 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8401 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8461 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8521 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8581 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8641 GCGAATTTT
//