LOCUS       RC214776                8250 bp    DNA     circular  SYN 
DEFINITION  MYBPC1 (Myc-DDK-tagged)-Human myosin binding protein C, slow type (MYBPC1), transcript variant 4
ACCESSION   NM_206821
VERSION     NM_206821.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4407..4498 
                     /label="Myc-DDK"  
     misc_feature    4528..5113 
                     /label="PolyA signal"  
     misc_feature    4585..4604 
                     /label="Restriction Enzyme"  
     misc_feature    5389..6061 
                     /label="ColE1"  
     misc_feature    6491..7285 
                     /label="Kan/Neo"  
     misc_feature    7320..7622 
                     /label="SV40 ori"  
     misc_feature    7708..8185 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4389..4395 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4389 
                     /label="MYBPC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCAGAACCC ACTAAGAAAG AGGAAAATGA AGTGCCAGCC CCAGCCCCAC 
     1081 CCCCGGAAGA ACCAAGTAAA GAGAAGGAGG CCGGAACTAC ACCAGCAAAA GACTGGACCC 
     1141 TTGTCGAAAC TCCTCCTGGG GAGGAACAAG CCAAGCAGAA TGCCAACTCC CAGCTGTCCA 
     1201 TCTTGTTCAT TGAAAAACCT CAAGGAGGAA CAGTGAAAGT TGGTGAAGAT ATCACCTTCA 
     1261 TAGCCAAAGT CAAGGCTGAA GATCTTCTGA GAAAACCCAC TATCAAATGG TTCAAAGGAA 
     1321 AATGGATGGA CCTGGCCAGC AAAGCCGGGA AGCACCTTCA GCTGAAGGAA ACCTTTGAGA 
     1381 GGCACAGTCG GGTGTACACA TTTGAGATGC AGATCATCAA GGCCAAAGAT AACTTTGCAG 
     1441 GAAATTACAG ATGCGAGGTC ACCTATAAGG ATAAGTTTGA CAGCTGTTCA TTTGATCTTG 
     1501 AAGTGCACGA ATCTACTGGG ACTACTCCAA ACATTGACAT CAGATCTGCT TTCAAGAGAA 
     1561 GTGGAGAAGG TCAAGAGGAT GCAGGAGAAC TTGACTTTAG TGGTCTCCTG AAACGTAGGG 
     1621 AGGTGAAGCA GCAGGAGGAA GAACCCCAGG TGGACGTATG GGAGTTGCTG AAGAACGCGA 
     1681 AACCCAGTGA GTACGAGAAG ATCGCCTTCC AGTATGGAAT CACCGACCTG CGCGGCATGC 
     1741 TCAAGCGACT CAAGCGCATG CGCAGAGAGG AGAAGAAGAG CGCAGCTTTT GCAAAAATTC 
     1801 TTGATCCTGC ATATCAGGTT GACAAAGGAG GCAGAGTGAG GTTTGTTGTG GAGCTGGCAG 
     1861 ATCCAAAGTT GGAGGTGAAA TGGTATAAAA ATGGTCAAGA AATTCGACCC AGTACCAAAT 
     1921 ACATCTTTGA ACACAAAGGA TGCCAGAGAA TCCTGTTTAT CAATAACTGT CAGATGACAG 
     1981 ATGATTCAGA GTATTATGTG ACAGCCGGTG ATGAGAAATG TTCCACTGAG CTCTTCGTAA 
     2041 GAGAGCCTCC AATTATGGTG ACCAAACAGC TGGAAGATAC AACTGCTTAT TGTGGGGAGA 
     2101 GAGTGGAATT AGAATGTGAG GTGTCTGAAG ATGATGCCAA TGTAAAATGG TTTAAGAATG 
     2161 GTGAAGAGAT TATCCCTGGT CCAAAATCAA GATACCGAAT TAGAGTTGAG GGTAAAAAAC 
     2221 ACATCTTGAT CATAGAGGGA GCAACAAAGG CTGATGCTGC AGAATATTCA GTAATGACAA 
     2281 CAGGAGGACA ATCATCTGCT AAACTTAGTG TTGACTTGAA ACCTCTGAAG ATTTTGACTC 
     2341 CTCTGACTGA TCAGACTGTA AATCTTGGAA AAGAAATCTG CCTGAAGTGT GAAATCTCTG 
     2401 AAAACATACC AGGAAAATGG ACTAAAAATG GCCTACCTGT TCAGGAGAGT GACCGTCTAA 
     2461 AGGTGGTTCA CAAGGGAAGG ATCCACAAGT TAGTGATAGC CAATGCCCTC ACTGAAGATG 
     2521 AAGGTGATTA TGTATTTGCA CCTGATGCCT ACAATGTTAC TCTGCCTGCC AAAGTTCATG 
     2581 TTATTGATCC TCCTAAGATC ATCCTGGATG GTCTTGATGC TGACAACACA GTGACAGTGA 
     2641 TTGCAGGAAA CAAGCTTCGT CTTGAGATCC CCATCAGCGG AGAACCACCT CCTAAAGCCA 
     2701 TGTGGAGCCG GGGAGATAAG GCTATTATGG AAGGCAGTGG CCGGATAAGA ACAGAATCTT 
     2761 ACCCTGATAG CAGCACTCTG GTCATTGATA TAGCTGAAAG AGATGACTCT GGTGTTTACC 
     2821 ACATCAATCT GAAAAACGAA GCTGGAGAGG CACATGCAAG CATCAAGGTT AAAGTTGTGG 
     2881 ACTTCCCTGA TCCTCCAGTG GCACCGACTG TGACAGAGGT GGGAGATGAC TGGTGTATCA 
     2941 TGAACTGGGA GCCTCCTGCC TACGACGGAG GCTCTCCAAT CCTAGGATAT TTTATTGAGA 
     3001 GGAAGAAGAA ACAAAGCTCC AGGTGGATGA GGCTGAATTT TGATCTCTGC AAAGAAACAA 
     3061 CTTTTGAGCC CAAGAAGATG ATTGAAGGTG TGGCCTATGA GGTCCGCATC TTTGCAGTCA 
     3121 ATGCCATTGG CATCTCCAAG CCCAGTATGC CCTCCAGGCC TTTTGTTCCT TTGGCTGTAA 
     3181 CAAGCCCTCC TACTCTTCTG ACTGTGGACT CTGTCACTGA CACGACTGTC ACGATGAGGT 
     3241 GGCGCCCCCC AGACCACATT GGTGCAGCAG GTTTAGATGG CTATGTGCTA GAGTATTGCT 
     3301 TTGAAGGAAC TGAGGACTGG ATAGTTGCAA ACAAAGATCT GATTGACAAG ACGAAGTTCA 
     3361 CCATCACAGG TCTGCCAACA GATGCAAAGA TCTTTGTGCG TGTGAAGGCT GTTAATGCAG 
     3421 CTGGTGCCAG CGAGCCCAAG TACTATTCTC AGCCCATTCT CGTGAAGGAA ATCATAGAAC 
     3481 CTCCAAAGAT TCGCATTCCA AGACACCTGA AGCAAACCTA TATCCGCAGA GTTGGAGAAG 
     3541 CTGTCAATCT GGTTATACCT TTCCAGGGAA AACCAAGACC AGAATTAACT TGGAAGAAGG 
     3601 ATGGTGCAGA AATTGATAAG AATCAAATAA ACATTCGCAA CTCTGAGACT GATACAATCA 
     3661 TATTTATTAG AAAAGCAGAG AGGAGCCACT CTGGGAAATA TGATCTGCAA GTCAAAGTGG 
     3721 ACAAATTCGT GGAGACCGCA TCAATTGACA TCCAGATCAT TGACCGTCCA GGTCCACCCC 
     3781 AAATTGTGAA GATTGAGGAT GTCTGGGGAG AAAATGTCGC TCTCACATGG ACTCCACCAA 
     3841 AGGATGATGG AAATGCTGCT ATCACAGGCT ATACCATTCA GAAGGCTGAC AAGAAGAGCA 
     3901 TGGAATGGTT TACTGTCATT GAGCATTATC ATCGAACCAG TGCCACCATT ACTGAATTGG 
     3961 TCATAGGGAA TGAATATTAC TTCCGGGTCT TTTCTGAAAA CATGTGTGGC CTCAGTGAGG 
     4021 ATGCCACCAT GACTAAAGAG AGTGCAGTGA TCGCCAGGGA TGGTAAAATC TACAAAAATC 
     4081 CAGTGTATGA AGACTTTGAT TTCTCAGAGG CACCCATGTT TACTCAGCCT TTGGTTAACA 
     4141 CCTATGCCAT AGCTGGTTAC AATGCCACCC TAAACTGCAG TGTGAGAGGA AATCCTAAGC 
     4201 CTAAAATAAC CTGGATGAAA AACAAAGTTG CTATTGTGGA TGATCCAAGA TACAGGATGT 
     4261 TCAGCAACCA GGGAGTCTGT ACCCTGGAAA TTCGCAAGCC CAGCCCCTAT GATGGAGGCA 
     4321 CTTACTGCTG CAAAGCAGTC AATGACCTTG GGACAGTGGA GATTGAATGC AAACTGGAGG 
     4381 TGAAAGTCAT TGCACAAACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4441 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4501 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4561 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4621 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4681 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4741 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4801 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4861 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4921 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4981 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5041 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5101 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5161 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5221 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5281 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5341 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5401 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5461 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5521 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5581 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5641 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5701 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5761 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5821 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5881 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5941 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6001 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6061 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6121 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6181 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6241 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6301 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6361 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6421 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6481 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6541 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6601 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6661 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6721 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6781 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6841 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6901 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6961 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7021 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7081 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7141 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7201 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7261 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7321 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7381 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7441 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7501 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7561 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7621 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7681 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7741 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7801 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7861 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7921 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7981 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8041 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8101 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8161 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8221 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//