LOCUS       RC214736                8649 bp    DNA     circular  SYN 
DEFINITION  GNPTAB (Myc-DDK-tagged)-Human N-acetylglucosamine-1-phosphate transferase, alpha and beta subunits (GNPTAB)
ACCESSION   NM_024312
VERSION     NM_024312.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4806..4897 
                     /label="Myc-DDK"  
     misc_feature    4927..5512 
                     /label="PolyA signal"  
     misc_feature    4984..5003 
                     /label="Restriction Enzyme"  
     misc_feature    5788..6460 
                     /label="ColE1"  
     misc_feature    6890..7684 
                     /label="Kan/Neo"  
     misc_feature    7719..8021 
                     /label="SV40 ori"  
     misc_feature    8107..8584 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4788..4794 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4788 
                     /label="GNPTAB"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGTTCAAG CTCCTGCAGA GACAGACCTA TACCTGCCTG TCCCACAGGT 
     1081 ATGGGCTCTA CGTGTGCTTC TTGGGCGTCG TTGTCACCAT CGTCTCCGCC TTCCAGTTCG 
     1141 GAGAGGTGGT TCTGGAATGG AGCCGAGATC AATACCATGT TTTGTTTGAT TCCTATAGAG 
     1201 ACAATATTGC TGGAAAGTCC TTTCAGAATC GGCTTTGTCT GCCCATGCCG ATTGACGTTG 
     1261 TTTACACCTG GGTGAATGGC ACAGATCTTG AACTACTGAA GGAACTACAG CAGGTCAGAG 
     1321 AACAGATGGA GGAGGAGCAG AAAGCAATGA GAGAAATCCT TGGGAAAAAC ACAACGGAAC 
     1381 CTACTAAGAA GAGTGAGAAG CAGTTAGAGT GTTTGCTAAC ACACTGCATT AAGGTGCCAA 
     1441 TGCTTGTCCT GGACCCAGCC CTGCCAGCCA ACATCACCCT GAAGGACCTG CCATCTCTTT 
     1501 ATCCTTCTTT TCATTCTGCC AGTGACATTT TCAATGTTGC AAAACCAAAA AACCCTTCTA 
     1561 CCAATGTCTC AGTTGTTGTT TTTGACAGTA CTAAGGATGT TGAAGATGCC CACTCTGGAC 
     1621 TGCTTAAAGG AAATAGCAGA CAGACAGTAT GGAGGGGCTA CTTGACAACA GATAAAGAAG 
     1681 TCCCTGGATT AGTGCTAATG CAAGATTTGG CTTTCCTGAG TGGATTTCCA CCAACATTCA 
     1741 AGGAAACAAA TCAACTAAAA ACAAAATTGC CAGAAAATCT TTCCTCTAAA GTCAAACTGT 
     1801 TGCAGTTGTA TTCAGAGGCC AGTGTAGCGC TTCTAAAACT GAATAACCCC AAGGATTTTC 
     1861 AAGAATTGAA TAAGCAAACT AAGAAGAACA TGACCATTGA TGGAAAAGAA CTGACCATAA 
     1921 GTCCTGCATA TTTATTATGG GATCTGAGCG CCATCAGCCA GTCTAAGCAG GATGAAGACA 
     1981 TCTCTGCCAG TCGTTTTGAA GATAACGAAG AACTGAGGTA CTCATTGCGA TCTATCGAGA 
     2041 GGCATGCACC ATGGGTTCGG AATATTTTCA TTGTCACCAA CGGGCAGATT CCATCCTGGC 
     2101 TGAACCTTGA CAATCCTCGA GTGACAATAG TAACACACCA GGATGTTTTT CGAAATTTGA 
     2161 GCCACTTGCC TACCTTTAGT TCACCTGCTA TTGAAAGTCA CATTCATCGC ATCGAAGGGC 
     2221 TGTCCCAGAA GTTTATTTAC CTAAATGATG ATGTCATGTT TGGGAAGGAT GTCTGGCCAG 
     2281 ATGATTTTTA CAGTCACTCC AAAGGCCAGA AGGTTTATTT GACATGGCCT GTGCCAAACT 
     2341 GTGCCGAGGG CTGCCCAGGT TCCTGGATTA AGGATGGCTA TTGTGACAAG GCTTGTAATA 
     2401 ATTCAGCCTG CGATTGGGAT GGTGGGGATT GCTCTGGAAA CAGTGGAGGG AGTCGCTATA 
     2461 TTGCAGGAGG TGGAGGTACT GGGAGTATTG GAGTTGGACA GCCCTGGCAG TTTGGTGGAG 
     2521 GAATAAACAG TGTCTCTTAC TGTAATCAGG GATGTGCGAA TTCCTGGCTC GCTGATAAGT 
     2581 TCTGTGACCA AGCATGCAAT GTCTTGTCCT GTGGGTTTGA TGCTGGCGAC TGTGGGCAAG 
     2641 ATCATTTTCA TGAATTGTAT AAAGTGATCC TTCTCCCAAA CCAGACTCAC TATATTATTC 
     2701 CAAAAGGTGA ATGCCTGCCT TATTTCAGCT TTGCAGAAGT AGCCAAAAGA GGAGTTGAAG 
     2761 GTGCCTATAG TGACAATCCA ATAATTCGAC ATGCTTCTAT TGCCAACAAG TGGAAAACCA 
     2821 TCCACCTCAT AATGCACAGT GGAATGAATG CCACCACAAT ACATTTTAAT CTCACGTTTC 
     2881 AAAATACAAA CGATGAAGAG TTCAAAATGC AGATAACAGT GGAGGTGGAC ACAAGGGAGG 
     2941 GACCAAAACT GAATTCTACG GCCCAGAAGG GTTACGAAAA TTTAGTTAGT CCCATAACAC 
     3001 TTCTTCCAGA GGCGGAAATC CTTTTTGAGG ATATTCCCAA AGAAAAACGC TTCCCGAAGT 
     3061 TTAAGAGACA TGATGTTAAC TCAACAAGGA GAGCCCAGGA AGAGGTGAAA ATTCCCCTGG 
     3121 TAAATATTTC ACTCCTTCCA AAAGACGCCC AGTTGAGTCT CAATACCTTG GATTTGCAAC 
     3181 TGGAACATGG AGACATCACT TTGAAAGGAT ACAATTTGTC CAAGTCAGCC TTGCTGAGAT 
     3241 CATTTCTGAT GAACTCACAG CATGCTAAAA TAAAAAATCA AGCTATAATA ACAGATGAAA 
     3301 CAAATGACAG TTTGGTGGCT CCACAGGAAA AACAGGTTCA TAAAAGCATC TTGCCAAACA 
     3361 GCTTAGGAGT GTCTGAAAGA TTGCAGAGGT TGACTTTTCC TGCAGTGAGT GTAAAAGTGA 
     3421 ATGGTCATGA CCAGGGTCAG AATCCACCCC TGGACTTGGA GACCACAGCA AGATTTAGAG 
     3481 TGGAAACTCA CACCCAAAAA ACCATAGGCG GAAATGTGAC AAAAGAAAAG CCCCCATCTC 
     3541 TGATTGTTCC ACTGGAAAGC CAGATGACAA AAGAAAAGAA AATCACAGGG AAAGAAAAAG 
     3601 AGAACAGTAG AATGGAGGAA AATGCTGAAA ATCACATAGG CGTTACTGAA GTGTTACTTG 
     3661 GAAGAAAGCT GCAGCATTAC ACAGATAGTT ACTTGGGCTT TTTGCCATGG GAGAAAAAAA 
     3721 AGTATTTCCA AGATCTTCTC GACGAAGAAG AGTCATTGAA GACACAATTG GCATACTTCA 
     3781 CTGATAGCAA AAATACTGGG AGGCAACTAA AAGATACATT TGCAGATTCC CTCAGATATG 
     3841 TAAATAAAAT TCTAAATAGC AAGTTTGGAT TCACATCGCG GAAAGTCCCT GCTCACATGC 
     3901 CTCACATGAT TGACCGGATT GTTATGCAAG AACTGCAAGA TATGTTCCCT GAAGAATTTG 
     3961 ACAAGACGTC ATTTCACAAA GTGCGCCATT CTGAGGATAT GCAGTTTGCC TTCTCTTATT 
     4021 TTTATTATCT CATGAGTGCA GTGCAGCCAC TGAATATATC TCAAGTCTTT GATGAAGTTG 
     4081 ATACAGATCA ATCTGGTGTC TTGTCTGACA GAGAAATCCG AACACTGGCT ACCAGAATTC 
     4141 ACGAACTGCC GTTAAGTTTG CAGGATTTGA CAGGTCTGGA ACACATGCTA ATAAATTGCT 
     4201 CAAAAATGCT TCCTGCTGAT ATCACGCAGC TAAATAATAT TCCACCAACT CAGGAATCCT 
     4261 ACTATGATCC CAACCTGCCA CCGGTCACTA AAAGTCTAGT AACAAACTGT AAACCAGTAA 
     4321 CTGACAAAAT CCACAAAGCA TATAAGGACA AAAACAAATA TAGGTTTGAA ATCATGGGAG 
     4381 AAGAAGAAAT CGCTTTTAAA ATGATTCGTA CCAACGTTTC TCATGTGGTT GGCCAGTTGG 
     4441 ATGACATAAG AAAAAACCCT AGGAAGTTTG TTTGCCTGAA TGACAACATT GACCACAATC 
     4501 ATAAAGATGC TCAGACAGTG AAGGCTGTTC TCAGGGACTT CTATGAATCC ATGTTCCCCA 
     4561 TACCTTCCCA ATTTGAACTG CCAAGAGAGT ATCGAAACCG TTTCCTTCAT ATGCATGAGC 
     4621 TGCAGGAATG GAGGGCTTAT CGAGACAAAT TGAAGTTTTG GACCCATTGT GTACTAGCAA 
     4681 CATTGATTAT GTTTACTATA TTCTCATTTT TTGCTGAGCA GTTAATTGCA CTTAAGCGGA 
     4741 AGATATTTCC CAGAAGGAGG ATACACAAAG AAGCTAGTCC CAATCGAATC AGAGTAACGC 
     4801 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4861 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4921 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4981 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5041 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5101 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5161 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5221 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5281 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5341 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5401 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5461 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5521 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5581 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5641 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5701 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5761 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5821 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5881 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5941 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6001 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6061 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6121 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6181 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6241 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6301 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6361 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6421 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6481 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6541 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6601 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6661 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6721 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6781 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6841 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6901 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6961 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7021 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7081 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7141 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7201 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7261 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7321 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7381 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7441 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7501 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7561 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7621 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7681 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7741 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7801 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7861 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7921 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7981 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8041 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8101 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8161 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8221 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8281 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8341 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8401 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8461 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8521 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8581 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8641 GCGAATTTT
//