LOCUS       RC214653L1                9318 bp    DNA     circular  SYN 
DEFINITION  EML4 (Myc-DDK-tagged)-Human echinoderm microtubule associated protein like 4 (EML4), transcript variant 1
ACCESSION   NM_019063
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7609..7789 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8517..9176 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7948..8516 
                     /label="ColE1"  
     misc_feature    6484..6504 
                     /label="LR50 primer"  
     misc_feature    6281..6352 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6493..7084 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6263..6269 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6263 
                     /label="EML4"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGACGGTTTC GCCGGCAGTC TCGATGATAG 
     3361 TATTTCTGCT GCAAGTACTT CTGATGTTCA AGATCGCCTG TCAGCTCTTG AGTCACGAGT 
     3421 TCAGCAACAA GAAGATGAAA TCACTGTGCT AAAGGCGGCT TTGGCTGATG TTTTGAGGCG 
     3481 TCTTGCAATC TCTGAAGATC ATGTGGCCTC AGTGAAAAAA TCAGTCTCAA GTAAAGGCCA 
     3541 ACCAAGCCCT CGAGCAGTTA TTCCCATGTC CTGTATAACC AATGGAAGTG GTGCAAACAG 
     3601 AAAACCAAGT CATACCAGTG CTGTCTCAAT TGCAGGAAAA GAAACTCTTT CATCTGCTGC 
     3661 TAAAAGTGGT ACAGAAAAAA AGAAAGAAAA ACCACAAGGA CAGAGAGAAA AAAAAGAGGA 
     3721 ATCTCATTCT AATGATCAAA GTCCACAAAT TCGAGCATCA CCTTCTCCCC AGCCCTCTTC 
     3781 ACAACCTCTC CAAATACACA GACAAACTCC AGAAAGCAAG AATGCTACTC CCACCAAAAG 
     3841 CATAAAACGA CCATCACCAG CTGAAAAGTC ACATAATTCT TGGGAAAATT CAGATGATAG 
     3901 CCGTAATAAA TTGTCGAAAA TACCTTCAAC ACCCAAATTA ATACCAAAAG TTACCAAAAC 
     3961 TGCAGACAAG CATAAAGATG TCATCATCAA CCAAGAAGGA GAATATATTA AAATGTTTAT 
     4021 GCGCGGTCGG CCAATTACCA TGTTCATTCC TTCCGATGTT GACAACTATG ATGACATCAG 
     4081 AACGGAACTG CCTCCTGAGA AGCTCAAACT GGAGTGGGCA TATGGTTATC GAGGAAAGGA 
     4141 CTGTAGAGCT AATGTTTACC TTCTTCCGAC CGGGGAAATA GTTTATTTCA TTGCATCAGT 
     4201 AGTAGTACTA TTTAATTATG AGGAGAGAAC TCAGCGACAC TACCTGGGCC ATACAGACTG 
     4261 TGTGAAATGC CTTGCTATAC ATCCTGACAA AATTAGGATT GCAACTGGAC AGATAGCTGG 
     4321 CGTGGATAAA GATGGAAGGC CTCTACAACC CCACGTCAGA GTGTGGGATT CTGTTACTCT 
     4381 ATCCACACTG CAGATTATTG GACTTGGCAC TTTTGAGCGT GGAGTAGGAT GCCTGGATTT 
     4441 TTCAAAAGCA GATTCAGGTG TTCATTTATG TGTTATTGAT GACTCCAATG AGCATATGCT 
     4501 TACTGTATGG GACTGGCAGA AGAAAGCAAA AGGAGCAGAA ATAAAGACAA CAAATGAAGT 
     4561 TGTTTTGGCT GTGGAGTTTC ACCCAACAGA TGCAAATACC ATAATTACAT GCGGTAAATC 
     4621 TCATATTTTC TTCTGGACCT GGAGCGGCAA TTCACTAACA AGAAAACAGG GAATTTTTGG 
     4681 GAAATATGAA AAGCCAAAAT TTGTGCAGTG TTTAGCATTC TTGGGGAATG GAGATGTTCT 
     4741 TACTGGAGAC TCAGGTGGAG TCATGCTTAT ATGGAGCAAA ACTACTGTAG AGCCCACACC 
     4801 TGGGAAAGGA CCTAAAGGTG TATATCAAAT CAGCAAACAA ATCAAAGCTC ATGATGGCAG 
     4861 TGTGTTCACA CTTTGTCAGA TGAGAAATGG GATGTTATTA ACTGGAGGAG GGAAAGACAG 
     4921 AAAAATAATT CTGTGGGATC ATGATCTGAA TCCTGAAAGA GAAATAGAGG TTCCTGATCA 
     4981 GTATGGCACA ATCAGAGCTG TAGCAGAAGG AAAGGCAGAT CAATTTTTAG TAGGCACATC 
     5041 ACGAAACTTT ATTTTACGAG GAACATTTAA TGATGGCTTC CAAATAGAAG TACAGGGTCA 
     5101 TACAGATGAG CTTTGGGGTC TTGCCACACA TCCCTTCAAA GATTTGCTCT TGACATGTGC 
     5161 TCAGGACAGG CAGGTGTGCC TGTGGAACTC AATGGAACAC AGGCTGGAAT GGACCAGGCT 
     5221 GGTAGATGAA CCAGGACACT GTGCAGATTT TCATCCAAGT GGCACAGTGG TGGCCATAGG 
     5281 AACGCACTCA GGCAGGTGGT TTGTTCTGGA TGCAGAAACC AGAGATCTAG TTTCTATCCA 
     5341 CACAGACGGG AATGAACAGC TCTCTGTGAT GCGCTACTCA ATAGATGGTA CCTTCCTGGC 
     5401 TGTAGGATCT CATGACAACT TTATTTACCT CTATGTAGTC TCTGAAAATG GAAGAAAATA 
     5461 TAGCAGATAT GGAAGGTGCA CTGGACATTC CAGCTACATC ACACACCTTG ACTGGTCCCC 
     5521 AGACAACAAG TATATAATGT CTAACTCGGG AGACTATGAA ATATTGTACT GGGACATTCC 
     5581 AAATGGCTGC AAACTAATCA GGAATCGATC GGATTGTAAG GACATTGATT GGACGACATA 
     5641 TACCTGTGTG CTAGGATTTC AAGTATTTGG TGTCTGGCCA GAAGGATCTG ATGGGACAGA 
     5701 TATCAATGCA CTGGTGCGAT CCCACAATAG AAAGGTGATA GCTGTTGCCG ATGACTTTTG 
     5761 TAAAGTCCAT CTGTTTCAGT ATCCCTGCTC CAAAGCAAAG GCTCCCAGTC ACAAGTACAG 
     5821 TGCCCACAGC AGCCATGTCA CCAATGTCAG TTTTACTCAC AATGACAGTC ACCTGATATC 
     5881 AACTGGTGGA AAAGACATGA GCATCATTCA GTGGAAACTT GTGGAAAAGT TATCTTTGCC 
     5941 TCAGAATGAG ACTGTAGCGG ATACTACTCT AACCAAAGCC CCCGTCTCTT CCACTGAAAG 
     6001 TGTCATCCAA TCTAATACTC CCACACCGCC TCCTTCTCAG CCCTTAAATG AGACAGCTGA 
     6061 AGAGGAAAGT AGAATAAGCA GTTCTCCCAC ACTTCTGGAG AACAGCCTGG AACAAACTGT 
     6121 GGAGCCAAGT GAAGACCACA GCGAGGAGGA GAGTGAAGAG GGCAGCGGAG ACCTTGGTGA 
     6181 GCCTCTTTAT GAAGAGCCAT GCAACGAGAT AAGCAAGGAG CAGGCCAAAG CCACCCTTCT 
     6241 GGAGGACCAG CAAGACCCTT CGCCCTCGTC CACGCGTACG CGGCCGCTCG AGCAGAAACT 
     6301 CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA 
     6361 GGTTTAAACG GCCGGCCGCG GTCTGTACAA GTAGGATTCG TCGAGGGACC TAATAACTTC 
     6421 GTATAGCATA CATTATACGA AGTTATACAT GTTTAAGGGT TCCGGTTCCA CTAGGTACAA 
     6481 TTCGATATCA AGCTTATCGA TAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT 
     6541 GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG 
     6601 TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG 
     6661 CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG 
     6721 TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG 
     6781 ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC 
     6841 TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAATCA 
     6901 TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC 
     6961 TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT 
     7021 CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC 
     7081 GCCTCCCCGC ATCGATACCG TCGACCTCGA TCGAGACCTA GAAAAACATG GAGCAATCAC 
     7141 AAGTAGCAAT ACAGCAGCTA CCAATGCTGA TTGTGCCTGG CTAGAAGCAC AAGAGGAGGA 
     7201 GGAGGTGGGT TTTCCAGTCA CACCTCAGGT ACCTTTAAGA CCAATGACTT ACAAGGCAGC 
     7261 TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG GAAGGGCTAA TTCACTCCCA 
     7321 ACGAAGACAA GATATCCTTG ATCTGTGGAT CTACCACACA CAAGGCTACT TCCCTGATTG 
     7381 GCAGAACTAC ACACCAGGGC CAGGGATCAG ATATCCACTG ACCTTTGGAT GGTGCTACAA 
     7441 GCTAGTACCA GTTGAGCAAG AGAAGGTAGA AGAAGCCAAT GAAGGAGAGA ACACCCGCTT 
     7501 GTTACACCCT GTGAGCCTGC ATGGGATGGA TGACCCGGAG AGAGAAGTAT TAGAGTGGAG 
     7561 GTTTGACAGC CGCCTAGCAT TTCATCACAT GGCCCGAGAG CTGCATCCGG ACTGTACTGG 
     7621 GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT 
     7681 GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG 
     7741 TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG 
     7801 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     7861 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     7921 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     7981 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     8041 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     8101 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     8161 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     8221 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     8281 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     8341 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     8401 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     8461 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     8521 CATGATTACG CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG 
     8581 CCGACATGGA AGCCATCACA AACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC 
     8641 TTGTCGCCTT GCGTATAATA TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA 
     8701 TTGGCCACGT TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA GACGAAAAAC 
     8761 ATATTCTCAA TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT 
     8821 TGCGAATATA TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA 
     8881 AACGTTTCAG TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC 
     8941 AGCTCACCGT CTTTCATTGC CATACGGAAC TCCGGATGAG CATTCATCAG GCGGGCAAGA 
     9001 ATGTGAATAA AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC 
     9061 GTAATATCCA GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA 
     9121 AAATGTTCTT TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC 
     9181 TCCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     9241 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTCCCGCG CACATTTCCC 
     9301 CGAAAAGTGC CACCTGAC
//