LOCUS       RC214592                8214 bp    DNA     circular  SYN 
DEFINITION  WWC1 (Myc-DDK-tagged)-Human WW and C2 domain containing 1 (WWC1), transcript variant 3
ACCESSION   NM_015238
VERSION     NM_015238.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4371..4462 
                     /label="Myc-DDK"  
     misc_feature    4492..5077 
                     /label="PolyA signal"  
     misc_feature    4549..4568 
                     /label="Restriction Enzyme"  
     misc_feature    5353..6025 
                     /label="ColE1"  
     misc_feature    6455..7249 
                     /label="Kan/Neo"  
     misc_feature    7284..7586 
                     /label="SV40 ori"  
     misc_feature    7672..8149 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4353..4359 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4353 
                     /label="WWC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCCGGCCG GAGCTGCCCC TGCCGGAGGG CTGGGAGGAG GCGCGCGACT 
     1081 TCGACGGCAA GGTCTACTAC ATAGACCACA CGAACCGCAC CACCAGCTGG ATCGACCCGC 
     1141 GGGACAGGTA CACCAAACCG CTCACCTTTG CTGACTGCAT TAGTGATGAG TTGCCGCTAG 
     1201 GATGGGAAGA GGCATATGAC CCACAGGTTG GAGATTACTT CATAGACCAC AACACCAAAA 
     1261 CCACTCAGAT TGAGGATCCT CGAGTACAAT GGCGGCGGGA GCAGGAACAT ATGCTGAAGG 
     1321 ATTACCTGGT GGTGGCCCAG GAGGCTCTGA GTGCACAAAA GGAGATCTAC CAGGTGAAGC 
     1381 AGCAGCGCCT GGAGCTTGCA CAGCAGGAGT ACCAGCAACT GCATGCCGTC TGGGAGCATA 
     1441 AGCTGGGCTC CCAGGTCAGC TTGGTCTCTG GTTCATCATC CAGCTCCAAG TATGACCCTG 
     1501 AGATCCTGAA AGCTGAAATT GCCACTGCAA AATCCCGGGT CAACAAGCTG AAGAGAGAGA 
     1561 TGGTTCACCT CCAGCACGAG CTGCAGTTCA AAGAGCGTGG CTTTCAGACC CTGAAGAAAA 
     1621 TCGATAAGAA AATGTCTGAT GCTCAGGGCA GCTACAAACT GGATGAAGCT CAGGCTGTCT 
     1681 TGAGAGAAAC AAAAGCCATC AAAAAGGCTA TTACCTGTGG GGAAAAGGAA AAGCAAGATC 
     1741 TCATTAAGAG CCTTGCCATG TTGAAGGACG GCTTCCGCAC TGACAGGGGG TCTCACTCAG 
     1801 ACCTGTGGTC CAGCAGCAGC TCTCTGGAGA GTTCGAGTTT CCCGCTACCG AAACAGTACC 
     1861 TGGATGTGAG CTCCCAGACA GACATCTCGG GAAGCTTCGG CATCAACAGC AACAATCAGT 
     1921 TGGCAGAGAA GGTCAGATTG CGCCTTCGAT ATGAAGAGGC TAAGAGAAGG ATCGCCAACC 
     1981 TGAAGATCCA GCTGGCCAAG CTTGACAGTG AGGCCTGGCC TGGGGTGCTG GACTCAGAGA 
     2041 GGGACCGGCT GATCCTTATC AACGAGAAGG AGGAGCTGCT GAAGGAGATG CGCTTCATCA 
     2101 GCCCCCGCAA GTGGACCCAG GGGGAGGTGG AGCAGCTGGA GATGGCCCGG AAGCGGCTGG 
     2161 AAAAGGACCT GCAGGCAGCC CGGGACACCC AGAGCAAGGC GCTGACGGAG AGGTTAAAGT 
     2221 TAAACAGTAA GAGGAACCAG CTTGTGAGAG AACTGGAGGA AGCCACCCGG CAGGTGGCAA 
     2281 CTCTGCACTC CCAGCTGAAA AGTCTCTCAA GCAGCATGCA GTCCCTGTCC TCAGGCAGCA 
     2341 GCCCCGGATC CCTCACGTCC AGCCGGGGCT CCCTGGTTGC ATCCAGCCTG GACTCCTCCA 
     2401 CTTCAGCCAG CTTCACTGAC CTCTACTATG ACCCCTTTGA GCAGCTGGAC TCAGAGCTGC 
     2461 AGAGCAAGGT GGAGTTCCTG CTCCTGGAGG GGGCCACCGG CTTCCGGCCC TCGGGCTGCA 
     2521 TCACCACCAT CCACGAGGAT GAGGTGGCCA AGACCCAGAA GGCAGAGGGA GGTGGCCGCC 
     2581 TGCAGGCTCT GCGTTCCCTG TCTGGCACCC CAAAGTCCAT GACCTCCCTA TCCCCACGTT 
     2641 CCTCTCTCTC CTCCCCCTCC CCACCCTGTT CCCCTCTCAT GGCTGACCCC CTCCTGGCTG 
     2701 GTGATGCCTT CCTCAACTCC TTGGAGTTTG AAGACCCGGA GCTGAGTGCC ACTCTTTGTG 
     2761 AACTGAGCCT TGGTAACAGC GCCCAGGAAA GATACCGGCT GGAGGAACCA GGAACGGAGG 
     2821 GCAAGCAGCT GGGCCAAGCT GTGAATACGG CCCAGGGGTG TGGCCTGAAA GTGGCCTGTG 
     2881 TCTCAGCCGC CGTATCGGAC GAGTCAGTGG CTGGAGACAG TGGTGTGTAC GAGGCTTCCG 
     2941 TGCAGAGACT GGGTGCTTCA GAAGCTGCTG CATTTGACAG TGACGAATCG GAAGCAGTGG 
     3001 GTGCGACCCG AATTCAGATT GCCCTGAAGT ATGATGAGAA GAATAAGCAA TTTGCAATAT 
     3061 TAATCATCCA GCTGAGTAAC CTTTCTGCTC TGTTGCAGCA ACAAGACCAG AAAGTGAATA 
     3121 TCCGCGTGGC TGTCCTTCCT TGCTCTGAAA GCACAACCTG CCTGTTCCGG ACCCGGCCTC 
     3181 TGGACGCCTC AGACACTCTA GTGTTCAATG AGGTGTTCTG GGTATCCATG TCCTATCCAG 
     3241 CCCTTCACCA GAAGACCTTA AGAGTCGATG TCTGTACCAC CGACAGGAGC CATCTGGAAG 
     3301 AGTGCCTGGG AGGCGCCCAG ATCAGCCTGG CGGAGGTCTG CCGGTCTGGG GAGAGGTCGA 
     3361 CTCGCTGGTA CAACCTTCTC AGCTACAAAT ACTTGAAGAA GCAGAGCAGG GAGCTCAAGC 
     3421 CAGTGGGAGT TATGGCCCCT GCCTCAGGGC CTGCCAGCAC GGACGCTGTG TCTGCTCTGT 
     3481 TGGAACAGAC AGCAGTGGAG CTGGAGAAGA GGCAGGAGGG CAGGAGCAGC ACACAGACAC 
     3541 TGGAAGACAG CTGGAGGTAT GAGGAGACCA GTGAGAATGA GGCAGTAGCC GAGGAAGAGG 
     3601 AGGAGGAGGT GGAGGAGGAG GGAGAAGAGG ATGTTTTCAC CGAGAAAGCC TCACCTGATA 
     3661 TGGATGGGTA CCCAGCATTA AAGGTGGACA AAGAGACCAA CACGGAGACC CCGGCCCCAT 
     3721 CCCCCACAGT GGTGCGACCT AAGGACCGGA GAGTGGGCAC CCCGTCCCAG GGGCCATTTC 
     3781 TTCGAGGGAG CACCATCATC CGCTCTAAGA CCTTCTCCCC AGGACCCCAG AGCCAGTACG 
     3841 TGTGCCGGCT GAATCGGAGT GATAGTGACA GCTCCACTCT GTCCAAAAAG CCACCTTTTG 
     3901 TTCGAAACTC CCTGGAGCGA CGCAGCGTCC GGATGAAGCG GCCTTCCTCG GTCAAGTCGC 
     3961 TGCGCTCCGA GCGTCTGATC CGTACCTCGC TGGACCTGGA GTTAGACCTG CAGGCGACAA 
     4021 GAACCTGGCA CAGCCAATTG ACCCAGGAGA TCTCGGTGCT GAAGGAGCTC AAGGAGCAGC 
     4081 TGGAACAAGC CAAGAGCCAC GGGGAGAAGG AGCTGCCACA GTGGTTGCGT GAGGACGAGC 
     4141 GTTTCCGCCT GCTGCTGAGG ATGCTGGAGA AGCGGATGGA CCGAGCGGAG CACAAGGGTG 
     4201 AGCTTCAGAC AGACAAGATG ATGAGGGCAG CTGCCAAGGA TGTGCACAGG CTCCGAGGCC 
     4261 AGAGCTGTAA GGAACCCCCA GAAGTTCAGT CTTTCAGGGA GAAGATGGCA TTTTTCACCC 
     4321 GGCCTCGGAT GAATATCCCA GCTCTCTCTG CAGATGACGT CACGCGTACG CGGCCGCTCG 
     4381 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4441 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4501 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4561 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4621 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4681 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4741 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4801 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4861 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4921 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4981 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5041 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5101 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5161 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5221 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5281 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5341 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5401 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5461 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5521 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5581 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5641 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5701 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5761 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5821 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5881 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5941 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6001 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6061 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6121 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6181 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6241 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6301 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6361 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6421 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6481 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6541 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6601 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6661 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6721 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6781 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6841 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6901 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6961 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7021 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7081 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7141 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7201 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7261 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7321 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7381 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7441 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7501 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7561 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7621 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7681 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7741 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7801 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7861 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7921 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7981 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8041 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8101 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8161 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//