LOCUS       RC214433                8526 bp    DNA     circular  SYN 
DEFINITION  GPR158 (Myc-DDK-tagged)-Human G protein-coupled receptor 158 (GPR158)
ACCESSION   NM_020752
VERSION     NM_020752.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4683..4774 
                     /label="Myc-DDK"  
     misc_feature    4804..5389 
                     /label="PolyA signal"  
     misc_feature    4861..4880 
                     /label="Restriction Enzyme"  
     misc_feature    5665..6337 
                     /label="ColE1"  
     misc_feature    6767..7561 
                     /label="Kan/Neo"  
     misc_feature    7596..7898 
                     /label="SV40 ori"  
     misc_feature    7984..8461 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4665..4671 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4665 
                     /label="GPR158"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGAGCCATG GCTTACCCCT TACTCCTCTG CCTCCTGCTT GCTCAGCTGG 
     1081 GATTGGGAGC TGTTGGCGCC AGCCGCGACC CCCAAGGACG GCCGGATTCC CCTCGAGAGA 
     1141 GGACCCCGAA GGGGAAGCCG CACGCCCAGC AGCCGGGTCG AGCCTCTGCC TCGGACTCCT 
     1201 CGGCTCCCTG GAGCCGCTCC ACCGATGGCA CCATCTTGGC GCAGAAACTC GCCGAGGAGG 
     1261 TGCCCATGGA CGTGGCCTCT TACCTCTACA CCGGGGACTC CCACCAGCTG AAGCGAGCCA 
     1321 ACTGCTCCGG CCGCTACGAG TTGGCGGGCC TGCCGGGGAA GTGGCCAGCC CTGGCCAGCG 
     1381 CGCACCCCTC CTTGCACCGG GCGCTGGACA CACTGACACA CGCCACCAAC TTCCTCAACG 
     1441 TGATGCTGCA GAGCAATAAG TCGCGGGAGC AGAACTTGCA GGACGACCTG GATTGGTACC 
     1501 AGGCGCTGGT GTGGAGCCTT CTGGAGGGCG AGCCCAGCAT CTCCCGGGCG GCCATCACCT 
     1561 TCAGCACCGA TTCGCTGTCC GCACCGGCCC CACAGGTCTT CCTCCAGGCC ACGCGCGAGG 
     1621 AGAGCCGCAT CCTGCTCCAA GACCTGTCCT CCTCCGCACC CCACCTGGCC AACGCCACTC 
     1681 TGGAGACCGA GTGGTTCCAC GGCCTCCGGC GCAAGTGGAG GCCCCACTTA CACCGCCGCG 
     1741 GCCCCAATCA GGGGCCCCGG GGCCTGGGCC ACAGCTGGCG GCGCAAGGAC GGGCTCGGCG 
     1801 GGGACAAGAG CCACTTCAAG TGGTCTCCGC CTTATCTGGA GTGCGAGAAC GGGAGTTACA 
     1861 AGCCCGGGTG GCTGGTTACT CTTTCCTCTG CCATCTACGG GTTGCAGCCT AACCTGGTCC 
     1921 CGGAATTCAG GGGTGTCATG AAAGTTGACA TAAATCTTCA GAAAGTGGAC ATTGACCAAT 
     1981 GCTCAAGTGA TGGCTGGTTT TCAGGAACTC ATAAATGCCA CCTCAACAAT TCAGAGTGTA 
     2041 TGCCAATTAA AGGCCTAGGA TTCGTTCTTG GAGCCTATGA GTGCATTTGC AAAGCAGGAT 
     2101 TCTATCATCC TGGAGTCTTA CCAGTGAACA ACTTTCGGAG AAGGGGTCCG GATCAGCATA 
     2161 TTTCAGGAAG TACAAAAGAT GTGTCAGAAG AAGCCTATGT CTGCCTACCT TGCAGGGAGG 
     2221 GCTGCCCCTT CTGTGCTGAT GACAGCCCAT GCTTCGTCCA GGAAGATAAG TATTTACGAC 
     2281 TTGCCATCAT CTCCTTCCAA GCCCTGTGTA TGCTGCTCGA CTTCGTTAGC ATGCTGGTGG 
     2341 TCTACCACTT TCGCAAAGCA AAGAGCATCC GGGCATCGGG CCTTATCCTG TTGGAAACGA 
     2401 TCCTTTTTGG ATCTCTGCTC CTATACTTTC CAGTTGTTAT TTTGTACTTT GAGCCAAGCA 
     2461 CATTTCGCTG TATTCTCCTA AGATGGGCTC GTCTTCTCGG TTTTGCTACT GTTTACGGAA 
     2521 CTGTCACTCT CAAACTTCAC AGGGTTTTGA AGGTGTTTCT TTCACGAACG GCTCAACGAA 
     2581 TTCCATATAT GACTGGCGGA CGGGTCATGA GGATGCTGGC AGTAATACTC TTGGTAGTGT 
     2641 TTTGGTTTCT CATTGGCTGG ACTTCATCTG TGTGCCAGAA TTTGGAGAAA CAGATTTCAC 
     2701 TTATTGGCCA GGGGAAAACA TCCGATCACC TCATCTTCAA TATGTGCCTC ATTGACCGCT 
     2761 GGGACTACAT GACAGCAGTT GCTGAATTTT TATTCCTCTT GTGGGGTGTT TATCTCTGCT 
     2821 ATGCAGTGCG GACAGTCCCA TCGGCATTCC ATGAGCCCCG CTATATGGCT GTTGCAGTTC 
     2881 ACAATGAGCT CATCATCTCT GCTATATTCC ATACAATTAG ATTTGTTCTT GCCTCAAGAC 
     2941 TTCAGTCTGA TTGGATGTTG ATGCTGTATT TTGCACATAC TCATTTGACT GTGACAGTCA 
     3001 CCATTGGGTT GCTTTTGATT CCAAAGTTTT CACATTCAAG CAATAACCCA CGAGATGATA 
     3061 TTGCTACAGA AGCATATGAG GATGAGCTAG ACATGGGCCG ATCTGGATCC TACCTGAACA 
     3121 GCAGTATCAA TTCAGCCTGG AGTGAGCACA GCTTGGATCC AGAGGACATT CGGGACGAGC 
     3181 TGAAAAAACT CTATGCCCAA CTGGAAATAT ATAAAAGAAA GAAGATGATC ACAAACAACC 
     3241 CCCACCTCCA GAAAAAGCGG TGCTCGAAGA AGGGCCTAGG TCGTTCCATC ATGAGACGCA 
     3301 TTACGGAGAT CCCAGAGACA GTCAGCCGGC AGTGCTCTAA AGAGGACAAG GAGGGCGCCG 
     3361 ACCATGGCAC AGCCAAAGGC ACTGCCCTCA TCAGGAAGAA CCCCCCAGAG TCTTCAGGGA 
     3421 ACACAGGGAA ATCCAAGGAG GAGACCCTGA AAAACCGAGT CTTCTCACTC AAGAAATCCC 
     3481 ACAGCACTTA TGACCACGTG AGAGACCAAA CGGAAGAGTC CAGTAGCCTA CCCACAGAAA 
     3541 GCCAAGAGGA GGAGACAACA GAAAATTCCA CACTGGAATC CCTGTCGGGT AAAAAACTAA 
     3601 CACAAAAACT AAAAGAAGAC AGCGAGGCTG AGTCCACGGA GTCGGTGCCG TTGGTGTGCA 
     3661 AGTCAGCAAG CGCTCACAAC CTCAGCTCAG AGAAGAAAAC TGGGCACCCA CGAACATCGA 
     3721 TGTTACAGAA GTCTCTCAGT GTCATAGCAA GCGCCAAGGA GAAGACTCTT GGATTAGCTG 
     3781 GGAAAACCCA AACAGCAGGT GTGGAAGAAC GCACTAAATC CCAGAAACCT TTGCCAAAAG 
     3841 ATAAAGAGAC AAACAGAAAT CACTCAAATT CTGATAACAC AGAGACTAAA GATCCTGCCC 
     3901 CCCAAAACTC AAATCCTGCG GAGGAGCCAA GAAAGCCTCA GAAATCTGGG ATTATGAAAC 
     3961 AACAAAGGGT CAACCCCACC ACTGCCAATT CTGACCTGAA CCCAGGCACC ACCCAGATGA 
     4021 AGGACAACTT TGACATTGGG GAGGTGTGTC CTTGGGAGGT TTATGACCTG ACCCCTGGTC 
     4081 CTGTGCCTTC AGAATCAAAA GTTCAAAAGC ACGTATCTAT TGTGGCTTCT GAAATGGAGA 
     4141 AAAACCCCAC TTTTTCCTTA AAGGAGAAAT CTCACCACAA GCCTAAGGCA GCTGAGGTTT 
     4201 GTCAGCAATC CAATCAGAAG CGCATAGATA AGGCTGAAGT ATGCCTTTGG GAGAGCCAAG 
     4261 GCCAGTCCAT TTTGGAAGAT GAGAAGCTTT TGATTTCCAA GACTCCAGTT CTCCCAGAGA 
     4321 GGGCAAAAGA GGAGAACGGA GGTCAGCCTC GTGCAGCCAA TGTGTGTGCT GGGCAGAGCG 
     4381 AAGAACTGCC CCCCAAAGCT GTAGCATCAA AAACAGAGAA TGAAAATCTC AACCAAATAG 
     4441 GACACCAGGA AAAAAAGACA TCTTCTTCTG AGGAGAATGT GCGTGGCTCC TATAACTCAA 
     4501 GTAATAACTT CCAGCAACCT TTAACATCAC GAGCAGAGGT TTGTCCTTGG GAGTTTGAGA 
     4561 CCCCAGCTCA ACCAAATGCT GGAAGAAGTG TAGCTTTACC TGCCTCTTCT GCTCTAAGTG 
     4621 CAAATAAGAT AGCAGGGCCT AGGAAAGAAG AGATCTGGGA TAGTTTTAAA GTGACGCGTA 
     4681 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4741 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4801 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4861 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4921 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4981 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5041 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5101 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5161 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5221 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5281 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5341 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5401 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5461 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5521 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5581 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5641 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5701 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5761 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5821 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5881 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5941 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6001 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6061 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6121 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6181 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6241 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6301 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6361 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6421 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6481 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6541 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6601 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6661 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6721 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6781 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6841 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6901 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6961 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7021 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7081 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7141 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7201 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7261 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7321 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7381 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7441 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7501 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7561 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7621 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7681 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7741 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7801 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7861 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7921 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7981 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8041 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8101 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8161 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8221 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8281 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8341 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8401 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8461 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8521 AATTTT
//