LOCUS       RC214400                8463 bp    DNA     circular  SYN 
DEFINITION  GRM1 (Myc-DDK-tagged)-Human glutamate receptor, metabotropic 1 (GRM1), transcript variant 1
ACCESSION   NM_000838
VERSION     NM_000838.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4620..4711 
                     /label="Myc-DDK"  
     misc_feature    4741..5326 
                     /label="PolyA signal"  
     misc_feature    4798..4817 
                     /label="Restriction Enzyme"  
     misc_feature    5602..6274 
                     /label="ColE1"  
     misc_feature    6704..7498 
                     /label="Kan/Neo"  
     misc_feature    7533..7835 
                     /label="SV40 ori"  
     misc_feature    7921..8398 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4602..4608 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4602 
                     /label="GRM1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCGGGCTC CTTTTGTTTT TTTTCCCAGC GATCTTTTTG GAGGTGTCCC 
     1081 TTCTCCCCAG AAGCCCCGGC AGGAAAGTGT TGCTGGCAGG AGCGTCGTCT CAGCGCTCGG 
     1141 TGGCCAGAAT GGACGGAGAT GTCATCATTG GAGCCCTCTT CTCAGTCCAT CACCAGCCTC 
     1201 CGGCCGAGAA AGTGCCCGAG AGGAAGTGTG GGGAGATCAG GGAGCAGTAT GGCATCCAGA 
     1261 GGGTGGAGGC CATGTTCCAC ACGTTGGATA AGATCAACGC GGACCCGGTC CTCCTGCCCA 
     1321 ACATCACCCT GGGCAGTGAG ATCCGGGACT CCTGCTGGCA CTCTTCCGTG GCTCTGGAAC 
     1381 AGAGCATTGA GTTCATTAGG GACTCTCTGA TTTCCATTCG AGATGAGAAG GATGGGATCA 
     1441 ACCGGTGTCT GCCTGACGGC CAGTCCCTCC CCCCAGGCAG GACTAAGAAG CCCATTGCGG 
     1501 GAGTGATCGG TCCCGGCTCC AGCTCTGTAG CCATTCAAGT GCAGAACCTG CTCCAGCTCT 
     1561 TCGACATCCC CCAGATCGCT TATTCAGCCA CAAGCATCGA CCTGAGTGAC AAAACTTTGT 
     1621 ACAAATACTT CCTGAGGGTT GTCCCTTCTG ACACTTTGCA GGCAAGGGCC ATGCTTGACA 
     1681 TAGTCAAACG TTACAATTGG ACCTATGTCT CTGCAGTCCA CACGGAAGGG AATTATGGGG 
     1741 AGAGCGGAAT GGACGCTTTC AAAGAGCTGG CTGCCCAGGA AGGCCTCTGT ATCGCCCATT 
     1801 CTGACAAAAT CTACAGCAAC GCTGGGGAGA AGAGCTTTGA CCGACTCTTG CGCAAACTCC 
     1861 GAGAGAGGCT TCCCAAGGCT AGAGTGGTGG TCTGCTTCTG TGAAGGCATG ACAGTGCGAG 
     1921 GACTCCTGAG CGCCATGCGG CGCCTTGGCG TCGTGGGCGA GTTCTCACTC ATTGGAAGTG 
     1981 ATGGATGGGC AGACAGAGAT GAAGTCATTG AAGGTTATGA GGTGGAAGCC AACGGGGGAA 
     2041 TCACGATAAA GCTGCAGTCT CCAGAGGTCA GGTCATTTGA TGATTATTTC CTGAAACTGA 
     2101 GGCTGGACAC TAACACGAGG AATCCCTGGT TCCCTGAGTT CTGGCAACAT CGGTTCCAGT 
     2161 GCCGCCTTCC AGGACACCTT CTGGAAAATC CCAACTTTAA ACGAATCTGC ACAGGCAATG 
     2221 AAAGCTTAGA AGAAAACTAT GTCCAGGACA GTAAGATGGG GTTTGTCATC AATGCCATCT 
     2281 ATGCCATGGC ACATGGGCTG CAGAACATGC ACCATGCCCT CTGCCCTGGC CACGTGGGCC 
     2341 TCTGCGATGC CATGAAGCCC ATCGACGGCA GCAAGCTGCT GGACTTCCTC ATCAAGTCCT 
     2401 CATTCATTGG AGTATCTGGA GAGGAGGTGT GGTTTGATGA GAAAGGAGAC GCTCCTGGAA 
     2461 GGTATGATAT CATGAATCTG CAGTACACTG AAGCTAATCG CTATGACTAT GTGCACGTTG 
     2521 GAACCTGGCA TGAAGGAGTG CTGAACATTG ATGATTACAA AATCCAGATG AACAAGAGTG 
     2581 GAGTGGTGCG GTCTGTGTGC AGTGAGCCTT GCTTAAAGGG CCAGATTAAG GTTATACGGA 
     2641 AAGGAGAAGT GAGCTGCTGC TGGATTTGCA CGGCCTGCAA AGAGAATGAA TATGTGCAAG 
     2701 ATGAGTTCAC CTGCAAAGCT TGTGACTTGG GATGGTGGCC CAATGCAGAT CTAACAGGCT 
     2761 GTGAGCCCAT TCCTGTGCGC TATCTTGAGT GGAGCAACAT CGAATCCATT ATAGCCATCG 
     2821 CCTTTTCATG CCTGGGAATC CTTGTTACCT TGTTTGTCAC CCTAATCTTT GTACTGTACC 
     2881 GGGACACACC AGTGGTCAAA TCCTCCAGTC GGGAGCTCTG CTACATCATC CTAGCTGGCA 
     2941 TCTTCCTTGG TTATGTGTGC CCATTCACTC TCATTGCCAA ACCTACTACC ACCTCCTGCT 
     3001 ACCTCCAGCG CCTCTTGGTT GGCCTCTCCT CTGCGATGTG CTACTCTGCT TTAGTGACTA 
     3061 AAACCAATCG TATTGCACGC ATCCTGGCTG GCAGCAAGAA GAAGATCTGC ACCCGGAAGC 
     3121 CCAGGTTCAT GAGTGCCTGG GCTCAGGTGA TCATTGCCTC AATTCTGATT AGTGTGCAAC 
     3181 TAACCCTGGT GGTAACCCTG ATCATCATGG AACCCCCTAT GCCCATTCTG TCCTACCCAA 
     3241 GTATCAAGGA AGTCTACCTT ATCTGCAATA CCAGCAACCT GGGTGTGGTG GCCCCTTTGG 
     3301 GCTACAATGG ACTCCTCATC ATGAGCTGTA CCTACTATGC CTTCAAGACC CGCAACGTGC 
     3361 CCGCCAACTT CAACGAGGCC AAATATATCG CGTTCACCAT GTACACCACC TGTATCATCT 
     3421 GGCTAGCTTT TGTGCCCATT TACTTTGGGA GCAACTACAA GATCATCACA ACTTGCTTTG 
     3481 CAGTGAGTCT CAGTGTAACA GTGGCTCTGG GGTGCATGTT CACTCCCAAG ATGTACATCA 
     3541 TTATTGCCAA GCCTGAGAGG AATGTCCGCA GTGCCTTCAC CACCTCTGAT GTTGTCCGCA 
     3601 TGCATGTTGG CGATGGCAAG CTGCCCTGCC GCTCCAACAC TTTCCTCAAC ATCTTCCGAA 
     3661 GAAAGAAGGC AGGGGCAGGG AATGCCAATT CTAATGGCAA GTCTGTGTCA TGGTCTGAAC 
     3721 CAGGTGGAGG ACAGGTGCCC AAGGGACAGC ATATGTGGCA CCGCCTCTCT GTGCACGTGA 
     3781 AGACCAATGA GACGGCCTGC AACCAAACAG CCGTCATCAA GCCCCTCACT AAAAGTTACC 
     3841 AAGGCTCTGG CAAGAGCCTG ACCTTTTCAG ATACCAGCAC CAAGACCCTT TACAACGTAG 
     3901 AGGAGGAGGA GGATGCCCAG CCGATTCGCT TTAGCCCGCC TGGTAGCCCT TCCATGGTGG 
     3961 TGCACAGGCG CGTGCCAAGC GCGGCGACCA CTCCGCCTCT GCCGTCCCAC CTGACCGCAG 
     4021 AGGAGACCCC CCTCTTCCTG GCCGAACCAG CCCTCCCCAA GGGCTTGCCC CCTCCTCTCC 
     4081 AGCAGCAGCA GCAACCCCCT CCACAGCAGA AATCGCTGAT GGACCAGCTC CAGGGAGTGG 
     4141 TCAGCAACTT CAGTACCGCG ATCCCGGATT TTCACGCGGT GCTGGCAGGC CCCGGGGGTC 
     4201 CCGGGAACGG GCTGCGGTCC CTGTACCCGC CCCCGCCACC TCCGCAGCAC CTGCAGATGC 
     4261 TGCCGCTGCA GCTGAGCACC TTTGGGGAGG AGCTGGTCTC CCCGCCCGCG GACGACGACG 
     4321 ACGACAGCGA GAGGTTTAAG CTCCTCCAGG AGTACGTGTA TGAGCACGAG CGGGAAGGGA 
     4381 ACACGGAAGA AGACGAACTG GAAGAGGAGG AGGAGGACCT GCAGGCGGCC AGCAAACTGA 
     4441 CCCCGGATGA TTCGCCTGCG CTGACGCCTC CGTCGCCTTT CCGCGACTCG GTGGCCTCGG 
     4501 GCAGCTCGGT GCCCAGCTCC CCCGTGTCCG AGTCGGTGCT CTGCACCCCT CCCAACGTAT 
     4561 CCTACGCCTC TGTCATTCTG CGGGACTACA AGCAAAGCTC TTCCACCCTG ACGCGTACGC 
     4621 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4681 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4741 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4801 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4861 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4921 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4981 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5041 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5101 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5161 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5221 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5281 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5341 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5401 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5461 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5521 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5581 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5641 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5701 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5761 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5821 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5881 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5941 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6001 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6061 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6121 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6181 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6241 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6301 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6361 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6421 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6481 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6541 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6601 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6661 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6721 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6781 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6841 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6901 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6961 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7021 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7081 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7141 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7201 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7261 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7321 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7381 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7441 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7501 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7561 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7621 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7681 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7741 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7801 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7861 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7921 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7981 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8041 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8101 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8161 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8221 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8281 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8341 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8401 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8461 TTT
//