LOCUS       RC214353                8193 bp    DNA     circular  SYN 
DEFINITION  PARD3B (Myc-DDK-tagged)-Human par-3 partitioning defective 3 homolog B (C. elegans) (PARD3B), transcript variant a
ACCESSION   NM_205863
VERSION     NM_205863.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4350..4441 
                     /label="Myc-DDK"  
     misc_feature    4471..5056 
                     /label="PolyA signal"  
     misc_feature    4528..4547 
                     /label="Restriction Enzyme"  
     misc_feature    5332..6004 
                     /label="ColE1"  
     misc_feature    6434..7228 
                     /label="Kan/Neo"  
     misc_feature    7263..7565 
                     /label="SV40 ori"  
     misc_feature    7651..8128 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4332..4338 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4332 
                     /label="PARD3B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAGTGACC GTGTGCTTCG GCAGGACGGG CATCGTGGTG CCCTGCAAGG 
     1081 AGGGCCAGCT GCGCGTCGGC GAGCTCACCC AGCAGGCGCT GCAGCGGTAC CTGAAGACCC 
     1141 GGGAGAAGGG TCCTGGTTAC TGGGTGAAGA TTCATCACTT AGAATATACA GATGGAGGAA 
     1201 TCCTGGATCC AGATGATGTC TTGGCAGATG TTGTTGAAGA TAAAGACAAG CTGATTGCTG 
     1261 TGTTTGAAGA ACAAGAACCA CTCCACAAGA TTGAGAGCCC CAGTGGAAAC CCTGCAGATC 
     1321 GGCAGAGCCC AGATGCTTTT GAGACAGAAG TGGCCGCCCA ACTGGCCGCA TTTAAGCCAA 
     1381 TTGGTGGGGA GATTGAAGTA ACCCCTTCTG CTCTAAAACT AGGCACTCCA CTGCTGGTGA 
     1441 GGAGAAGCAG TGACCCAGTG CCAGGCCCAC CTGCTGATAC CCAGCCAAGC GCTTCACACC 
     1501 CTGGTGGCCA GAGTCTGAAA CTGGTTGTTC CAGATTCCAC GCAGAACTTG GAAGACAGAG 
     1561 AAGTTTTGAA TGGTGTACAG ACAGAACTAC TAACTTCGCC AAGAACTAAG GACACATTGA 
     1621 GTGATATGAC AAGAACAGTG GAGATTTCTG GGGAAGGAGG CCCATTGGGA ATACATGTAG 
     1681 TGCCCTTCTT TTCATCTCTG AGTGGAAGGA TTCTAGGACT CTTCATCCGA GGCATTGAAG 
     1741 ACAACAGCAG GTCCAAGCGG GAGGGACTAT TTCACGAAAA TGAATGTATT GTAAAAATCA 
     1801 ACAATGTGGA TCTCGTAGAC AAAACCTTTG CTCAGGCTCA AGATGTCTTC CGCCAGGCAA 
     1861 TGAAATCTCC AAGTGTGCTC CTCCACGTGC TTCCTCCACA AAACCGTGAA CAGTATGAAA 
     1921 AGTCAGTCAT TGGCTCTCTT AACATTTTTG GTAATAATGA TGGCGTTTTG AAAACCAAAG 
     1981 TGCCGCCTCC TGTCCATGGA AAATCGGGAC TAAAGACAGC AAATCTCACA GGAACCGATA 
     2041 GTCCTGAAAC AGATGCATCA GCTTCCCTGC AACAAAACAA GAGTCCCCGA GTACCAAGGC 
     2101 TGGGAGGAAA ACCATCCTCT CCCTCACTCT CGCCTCTCAT GGGATTTGGC AGCAATAAAA 
     2161 ATGCAAAGAA AATTAAGATT GACCTAAAGA AAGGCCCTGA AGGACTTGGT TTCACTGTGG 
     2221 TTACCAGAGA CTCTTCCATA CATGGTCCCG GTCCCATTTT TGTAAAAAAC ATTTTACCAA 
     2281 AGGGAGCAGC AATAAAAGAT GGCCGCCTAC AATCAGGGGA CAGAATTTTG GAGGTAAATG 
     2341 GGAGAGATGT CACCGGACGA ACCCAGGAAG AGCTTGTGGC CATGCTCAGG AGCACCAAGC 
     2401 AGGGGGAGAC AGCATCGCTG GTCATTGCCC GCCAAGAAGG ACATTTTCTG CCCCGAGAGT 
     2461 TGAAAGGAGA ACCTGACTGC TGTGCACTCT CTCTGGAGAC AAGCGAGCAG CTCACCTTTG 
     2521 AGATCCCCCT GAATGATTCA GGTTCTGCTG GCCTCGGGGT GAGCTTAAAA GGGAACAAAT 
     2581 CCAGAGAAAC TGGAACAGAC TTGGGGATTT TTATCAAATC CATCATTCAT GGAGGCGCTG 
     2641 CTTTTAAGGA TGGTCGTCTG CGAATGAATG ACCAGCTGAT TGCAGTTAAT GGGGAATCTC 
     2701 TTTTGGGAAA GTCCAACCAC GAAGCTATGG AAACACTTAG GCGGTCAATG TCCATGGAGG 
     2761 GAAACATCCG AGGGATGATC CAGTTGGTGA TTCTGAGGAG GCCAGAGAGA CCAATGGAGG 
     2821 ATCCTGCAGA GTGTGGGGCA TTTTCCAAGC CATGCTTTGA GAACTGTCAA AATGCTGTAA 
     2881 CCACCTCTAG GCGAAATGAT AATAGTATCC TGCATCCACT TGGCACTTGC AGTCCACAAG 
     2941 ACAAACAGAA AGGTCTATTG CTGCCCAATG ACGGATGGGC CGAGAGTGAA GTTCCACCTT 
     3001 CTCCAACACC ACATTCTGCT CTGGGATTGG GCCTCGAAGA TTACAGCCAC AGCTCTGGGG 
     3061 TGGATTCAGC AGTATATTTT CCAGATCAGC ACATCAACTT CAGATCTGTG ACACCGGCCA 
     3121 GGCAGCCTGA ATCAATTAAT TTGAAAGCCT CGAAGAGCAT GGACCTTGTG CCAGATGAAA 
     3181 GCAAGGTTCA CTCATTGGCT GGACAAAAAT CGGAATCTCC AAGCAAAGAT TTTGGTCCAA 
     3241 CTCTGGGTTT GAAAAAGTCC AGCTCCTTGG AGAGTCTGCA GACTGCAGTG GCCGAGGTCA 
     3301 GGAAGAATGA CCTTCCCTTT CACAGGCCCC GGCCGCACAT GGTTCGAGGC CGAGGCTGCA 
     3361 ATGAGAGCTT TAGAGCAGCC ATTGACAAAT CCTACGATGG ACCTGAAGAA ATAGAAGCTG 
     3421 ACGGTCTGTC TGATAAGAGC TCTCACTCTG GCCAAGGAGC TCTGAATTGT GAGTCTGCCC 
     3481 CTCAGGGGAA TTCGGAGCTA GAGGACATGG AAAATAAAGC CAGGAAAGTC AAAAAAACGA 
     3541 AAGAGAAGGA GAAGAAAAAG GAAAAGGGCA AATTGAAAGT CAAGGAGAAA AAGCGCAAAG 
     3601 AGGAGAATGA AGATCCAGAA AGGAAAATAA AGAAGAAGGG CTTCGGCGCC ATGCTGAGAT 
     3661 TTGGAAAGAA GAAAGAGGAT AAGGGTGGAA AGGCTGAGCA GAAAGGTACT CTGAAACATG 
     3721 GTGGCCTGAG AGAAGAAGAG CTGGAGAAAA TGAAAGAAGA GCGTGAAAGT GGCCGTCCTA 
     3781 CGGGTGGAAG CACTGACCGT ATCCAGAAGT TGCGGAAAGA GTATTATCAG GCTCGGAGGG 
     3841 AAGGTTTCCC TTTATATGAA GACGACGAAG GAAGAGCAAG GCCATCTGAG TATGACCTAC 
     3901 TCTGGGTGCC TGGAAGGGGT CCAGATGGGA ATGCACACAA CCTCCGCTTT GAAGGGATGG 
     3961 AGAGGCAGTA CGCATCCTTA CCCAGGGGAG GACCCGCAGA TCCTGTAGAC TATCTGCCAG 
     4021 CAGCACCTCG GGGGCTCTAC AAGGAAAGGG AGCTTCCCTA TTATCCAGGG GCTCATCCTA 
     4081 TGCACCCTCC CAAAGGGAGC TATCCCCGCC CCACAGAGCT CAGGGTGGCA GATCTCCGGT 
     4141 ATCCTCAGCA CTACCCACCC CCGCCAGCTC CCCAGCACAA AGGACCCTTT CGACAAGACG 
     4201 TTCCGCCTTC CCCTCCCCAG CACCAAAGAA TGCCAGCCTA TCAGGAAACA GGCAGACCAG 
     4261 GGCCCCGTGG GGGCAGCCCA GACCAGTACC CTTACCGAAC CCAGGATTCC CGGCAGAAGA 
     4321 ACCCCATGAC TGCAGCCGTA ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4381 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4441 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4501 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4561 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4621 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4681 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4741 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4801 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4861 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4921 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4981 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5041 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5101 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5161 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5221 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5281 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5341 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5401 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5461 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5521 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5581 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5641 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5701 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5761 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5821 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5881 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5941 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6001 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6061 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6121 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6181 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6241 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6301 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6361 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6421 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6481 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6541 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6601 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6661 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6721 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6781 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6841 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6901 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6961 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7021 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7081 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7141 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7201 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7261 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7321 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7381 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7441 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7501 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7561 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7621 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7681 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7741 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7801 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7861 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7921 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7981 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8041 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8101 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8161 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//