LOCUS       RC214344                8028 bp    DNA     circular  SYN 
DEFINITION  TAOK2 (Myc-DDK-tagged)-Human TAO kinase 2 (TAOK2), transcript variant 1
ACCESSION   NM_004783
VERSION     NM_004783.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4185..4276 
                     /label="Myc-DDK"  
     misc_feature    4306..4891 
                     /label="PolyA signal"  
     misc_feature    4363..4382 
                     /label="Restriction Enzyme"  
     misc_feature    5167..5839 
                     /label="ColE1"  
     misc_feature    6269..7063 
                     /label="Kan/Neo"  
     misc_feature    7098..7400 
                     /label="SV40 ori"  
     misc_feature    7486..7963 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4167..4173 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4167 
                     /label="TAOK2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCAGCTGGG GGCCGGGCCG GGAGCCTGAA GGACCCAGAT GTGGCTGAGC 
     1081 TCTTCTTCAA GGATGACCCA GAAAAGCTCT TCTCTGACCT CCGGGAAATT GGCCATGGCA 
     1141 GCTTTGGAGC CGTATACTTT GCCCGGGATG TCCGGAATAG TGAGGTGGTG GCCATCAAGA 
     1201 AGATGTCCTA CAGTGGGAAG CAGTCCAATG AGAAATGGCA AGACATCATC AAGGAGGTGC 
     1261 GGTTCTTACA GAAGCTCCGG CATCCCAACA CCATTCAGTA CCGGGGCTGT TACCTGAGGG 
     1321 AGCACACGGC TTGGCTGGTA ATGGAGTATT GCCTGGGCTC AGCTTCTGAC CTTCTAGAAG 
     1381 TGCACAAGAA ACCCCTTCAG GAGGTAGAGA TCGCAGCTGT GACCCACGGG GCGCTTCAGG 
     1441 GCCTGGCATA TCTGCACTCC CACAACATGA TCCATAGGGA TGTGAAGGCT GGAAACATCC 
     1501 TGCTGTCAGA GCCAGGGTTA GTGAAGCTAG GGGACTTTGG TTCTGCGTCC ATCATGGCAC 
     1561 CTGCCAACTC CTTCGTGGGC ACCCCATACT GGATGGCACC CGAGGTGATC CTGGCCATGG 
     1621 ATGAGGGGCA GTACGATGGC AAAGTGGACG TCTGGTCCTT GGGGATAACC TGCATCGAGC 
     1681 TGGCTGAACG GAAACCACCG CTCTTTAACA TGAATGCGAT GAGTGCCTTA TACCACATTG 
     1741 CACAGAACGA ATCCCCCGTG CTCCAGTCAG GACACTGGTC TGAGTACTTC CGGAATTTTG 
     1801 TCGACTCCTG TCTTCAGAAA ATCCCTCAAG ACAGACCAAC CTCAGAGGTT CTCCTGAAGC 
     1861 ACCGCTTTGT GCTCCGGGAG CGGCCACCCA CAGTCATCAT GGACCTGATC CAGAGGACCA 
     1921 AGGATGCCGT GCGGGAGCTG GACAACCTGC AGTACCGCAA GATGAAGAAG ATCCTGTTCC 
     1981 AAGAGGCACC CAACGGCCCT GGTGCCGAGG CCCCAGAGGA GGAAGAGGAG GCCGAGCCCT 
     2041 ACATGCACCG GGCCGGGACT CTGACCAGCC TCGAGAGTAG CCACTCAGTG CCCAGCATGT 
     2101 CCATCAGCGC CTCCAGCCAG AGCAGCTCCG TCAACAGCCT AGCAGATGCC TCAGACAACG 
     2161 AGGAAGAGGA GGAGGAGGAG GAGGAAGAGG AGGAGGAGGA AGAAGGCCCT GAAGCCCGGG 
     2221 AGATGGCCAT GATGCAGGAG GGGGAGCACA CAGTCACCTC TCACAGCTCC ATTATCCACC 
     2281 GGCTGCCGGG CTCTGACAAC CTATATGATG ACCCCTACCA GCCAGAGATA ACCCCCAGCC 
     2341 CTCTCCAGCC GCCTGCAGCC CCAGCTCCCA CTTCCACCAC CTCTTCCGCC CGCCGCCGGG 
     2401 CCTACTGCCG TAACCGAGAC CACTTTGCCA CCATCCGAAC CGCCTCCCTG GTCAGCCGTC 
     2461 AGATCCAGGA GCATGAGCAG GACTCTGCGC TGCGGGAGCA GCTGAGCGGC TATAAGCGGA 
     2521 TGCGACGACA GCACCAGAAG CAGCTGCTGG CCCTGGAGTC ACGGCTGAGG GGTGAACGGG 
     2581 AGGAGCACAG TGCACGGCTG CAGCGGGAGC TTGAGGCGCA GCGGGCTGGC TTTGGGGCAG 
     2641 AGGCAGAAAA GCTGGCCCGG CGGCACCAGG CCATAGGTGA GAAGGAGGCA CGAGCTGCCC 
     2701 AGGCCGAGGA GCGGAAGTTC CAGCAGCACA TCCTTGGGCA GCAGAAGAAG GAGCTGGCTG 
     2761 CCCTGCTGGA GGCACAGAAG CGGACCTACA AACTTCGCAA GGAACAGCTG AAGGAGGAGC 
     2821 TCCAGGAGAA CCCCAGCACT CCCAAGCGGG AGAAGGCCGA GTGGCTGCTG CGGCAGAAGG 
     2881 AGCAGCTCCA GCAGTGCCAG GCGGAGGAGG AAGCAGGGCT GCTGCGGCGG CAGCGCCAGT 
     2941 ACTTTGAGCT GCAGTGTCGC CAGTACAAGC GCAAGATGTT GCTGGCTCGG CACAGCCTGG 
     3001 ACCAGGACCT GCTGCGGGAG GACCTGAACA AGAAGCAGAC CCAGAAGGAC TTGGAGTGTG 
     3061 CACTGCTGCT TCGGCAGCAC GAGGCCACGC GGGAGCTGGA GCTGCGGCAG CTCCAGGCCG 
     3121 TGCAGCGCAC GCGGGCTGAG CTCACCCGCC TGCAGCACCA GACGGAGCTG GGCAACCAGC 
     3181 TGGAGTACAA CAAGCGGCGT GAGCAAGAGT TGCGGCAGAA GCATGCGGCC CAGGTTCGCC 
     3241 AGCAGCCCAA GAGCCTCAAA TCTAAGGAGC TGCAGATCAA GAAGCAGTTC CAGGAGACGT 
     3301 GTAAGATCCA GACTCGGCAG TACAAGGCTC TGCGAGCACA CTTGCTGGAG ACCACGCCCA 
     3361 AAGCTCAGCA CAAGAGCCTC CTTAAGCGGC TCAAGGAAGA GCAGACCCGC AAGCTGGCGA 
     3421 TCTTGGCGGA GCAGTATGAC CAGTCCATCT CAGAGATGCT CAGCTCACAG GCGCTGCGGC 
     3481 TTGATGAGAC CCAGGAGGCA GAGTTCCAGG CCCTTCGGCA GCAGCTTCAA CAGGAGCTGG 
     3541 AGCTGCTCAA CGCTTACCAG AGCAAGATCA AGATCCGCAC AGAGAGCCAG CACGAGAGGG 
     3601 AGCTGCGGGA GCTGGAGCAG AGGGTCGCGC TGCGGCGGGC ACTGCTGGAG CAGCGGGTGG 
     3661 AAGAGGAGCT GCTGGCCCTG CAGACAGGAC GCTCCGAGCG AATCCGCAGT CTGCTTGAGC 
     3721 GGCAGGCCCG TGAGATCGAG GCCTTCGATG CGGAAAGCAT GAGGCTGGGC TTCTCCAGCA 
     3781 TGGCTCTGGG GGGCATCCCG GCTGAAGCTG CTGCCCAGGG CTATCCTGCT CCACCCCCTG 
     3841 CCCCAGCCTG GCCCTCCCGT CCCGTTCCCC GTTCTGGGGC ACACTGGAGC CATGGCCCTC 
     3901 CTCCACCAGG CATGCCCCCT CCAGCCTGGC GTCAGCCGTC TCTGCTGGCT CCCCCAGGCC 
     3961 CCCCAAACTG GCTGGGGCCC CCCACACAAA GTGGGACACC CCGTGGCGGA GCCCTGCTGC 
     4021 TGCTAAGAAA CAGCCCCCAG CCCCTGCGGC GGGCAGCCTC GGGGGGCAGT GGCAGTGAGA 
     4081 ATGTGGGCCC CCCTGCTGCC GCGGTGCCCG GGCCCCTGAG CCGCAGCACC AGTGTCGCTT 
     4141 CCCACATCCT CAATGGTTCT TCCCACTTCT ATTCCACGCG TACGCGGCCG CTCGAGCAGA 
     4201 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4261 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4321 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4381 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4441 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4501 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4561 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4621 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4681 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4741 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4801 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4861 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4921 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4981 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5041 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5101 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5161 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5221 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5281 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5341 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5401 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5461 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5521 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5581 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5641 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5701 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5761 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5821 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5881 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5941 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6001 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6061 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6121 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6181 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6241 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6301 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6361 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6421 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6481 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6541 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6601 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6661 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6721 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6781 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6841 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6901 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6961 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7021 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7081 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7141 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7201 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7261 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7321 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7381 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7441 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7501 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7561 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7621 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7681 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7741 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7801 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7861 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7921 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7981 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//