LOCUS       RC214285                8970 bp    DNA     circular  SYN 
DEFINITION  FLT4 (Myc-DDK-tagged)-Human fms-related tyrosine kinase 4 (FLT4), transcript variant 1
ACCESSION   NM_182925
VERSION     NM_182925.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5127..5218 
                     /label="Myc-DDK"  
     misc_feature    5248..5833 
                     /label="PolyA signal"  
     misc_feature    5305..5324 
                     /label="Restriction Enzyme"  
     misc_feature    6109..6781 
                     /label="ColE1"  
     misc_feature    7211..8005 
                     /label="Kan/Neo"  
     misc_feature    8040..8342 
                     /label="SV40 ori"  
     misc_feature    8428..8905 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5109..5115 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5109 
                     /label="FLT4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGCGGGGC GCCGCGCTGT GCCTGCGACT GTGGCTCTGC CTGGGACTCC 
     1081 TGGACGGCCT GGTGAGTGGC TACTCCATGA CCCCCCCGAC CTTGAACATC ACGGAGGAGT 
     1141 CACACGTCAT CGACACCGGT GACAGCCTGT CCATCTCCTG CAGGGGACAG CACCCCCTCG 
     1201 AGTGGGCTTG GCCAGGAGCT CAGGAGGCGC CAGCCACCGG AGACAAGGAC AGCGAGGACA 
     1261 CGGGGGTGGT GCGAGACTGC GAGGGCACAG ACGCCAGGCC CTACTGCAAG GTGTTGCTGC 
     1321 TGCACGAGGT ACATGCCAAC GACACAGGCA GCTACGTCTG CTACTACAAG TACATCAAGG 
     1381 CACGCATCGA GGGCACCACG GCCGCCAGCT CCTACGTGTT CGTGAGAGAC TTTGAGCAGC 
     1441 CATTCATCAA CAAGCCTGAC ACGCTCTTGG TCAACAGGAA GGACGCCATG TGGGTGCCCT 
     1501 GTCTGGTGTC CATCCCCGGC CTCAATGTCA CGCTGCGCTC GCAAAGCTCG GTGCTGTGGC 
     1561 CAGACGGGCA GGAGGTGGTG TGGGATGACC GGCGGGGCAT GCTCGTGTCC ACGCCACTGC 
     1621 TGCACGATGC CCTGTACCTG CAGTGCGAGA CCACCTGGGG AGACCAGGAC TTCCTTTCCA 
     1681 ACCCCTTCCT GGTGCACATC ACAGGCAACG AGCTCTATGA CATCCAGCTG TTGCCCAGGA 
     1741 AGTCGCTGGA GCTGCTGGTA GGGGAGAAGC TGGTCCTGAA CTGCACCGTG TGGGCTGAGT 
     1801 TTAACTCAGG TGTCACCTTT GACTGGGACT ACCCAGGGAA GCAGGCAGAG CGGGGTAAGT 
     1861 GGGTGCCCGA GCGACGCTCC CAGCAGACCC ACACAGAACT CTCCAGCATC CTGACCATCC 
     1921 ACAACGTCAG CCAGCACGAC CTGGGCTCGT ATGTGTGCAA GGCCAACAAC GGCATCCAGC 
     1981 GATTTCGGGA GAGCACCGAG GTCATTGTGC ATGAAAATCC CTTCATCAGC GTCGAGTGGC 
     2041 TCAAAGGACC CATCCTGGAG GCCACGGCAG GAGACGAGCT GGTGAAGCTG CCCGTGAAGC 
     2101 TGGCAGCGTA CCCCCCGCCC GAGTTCCAGT GGTACAAGGA TGGAAAGGCA CTGTCCGGGC 
     2161 GCCACAGTCC ACATGCCCTG GTGCTCAAGG AGGTGACAGA GGCCAGCACA GGCACCTACA 
     2221 CCCTCGCCCT GTGGAACTCC GCTGCTGGCC TGAGGCGCAA CATCAGCCTG GAGCTGGTGG 
     2281 TGAATGTGCC CCCCCAGATA CATGAGAAGG AGGCCTCCTC CCCCAGCATC TACTCGCGTC 
     2341 ACAGCCGCCA GGCCCTCACC TGCACGGCCT ACGGGGTGCC CCTGCCTCTC AGCATCCAGT 
     2401 GGCACTGGCG GCCCTGGACA CCCTGCAAGA TGTTTGCCCA GCGTAGTCTC CGGCGGCGGC 
     2461 AGCAGCAAGA CCTCATGCCA CAGTGCCGTG ACTGGAGGGC GGTGACCACG CAGGATGCCG 
     2521 TGAACCCCAT CGAGAGCCTG GACACCTGGA CCGAGTTTGT GGAGGGAAAG AATAAGACTG 
     2581 TGAGCAAGCT GGTGATCCAG AATGCCAACG TGTCTGCCAT GTACAAGTGT GTGGTCTCCA 
     2641 ACAAGGTGGG CCAGGATGAG CGGCTCATCT ACTTCTATGT GACCACCATC CCCGACGGCT 
     2701 TCACCATCGA ATCCAAGCCA TCCGAGGAGC TACTAGAGGG CCAGCCGGTG CTCCTGAGCT 
     2761 GCCAAGCCGA CAGCTACAAG TACGAGCATC TGCGCTGGTA CCGCCTCAAC CTGTCCACGC 
     2821 TGCACGATGC GCACGGGAAC CCGCTTCTGC TCGACTGCAA GAACGTGCAT CTGTTCGCCA 
     2881 CCCCTCTGGC CGCCAGCCTG GAGGAGGTGG CACCTGGGGC GCGCCACGCC ACGCTCAGCC 
     2941 TGAGTATCCC CCGCGTCGCG CCCGAGCACG AGGGCCACTA TGTGTGCGAA GTGCAAGACC 
     3001 GGCGCAGCCA TGACAAGCAC TGCCACAAGA AGTACCTGTC GGTGCAGGCC CTGGAAGCCC 
     3061 CTCGGCTCAC GCAGAACTTG ACCGACCTCC TGGTGAACGT GAGCGACTCG CTGGAGATGC 
     3121 AGTGCTTGGT GGCCGGAGCG CACGCGCCCA GCATCGTGTG GTACAAAGAC GAGAGGCTGC 
     3181 TGGAGGAAAA GTCTGGAGTC GACTTGGCGG ACTCCAACCA GAAGCTGAGC ATCCAGCGCG 
     3241 TGCGCGAGGA GGATGCGGGA CGCTATCTGT GCAGCGTGTG CAACGCCAAG GGCTGCGTCA 
     3301 ACTCCTCCGC CAGCGTGGCC GTGGAAGGCT CCGAGGATAA GGGCAGCATG GAGATCGTGA 
     3361 TCCTTGTCGG TACCGGCGTC ATCGCTGTCT TCTTCTGGGT CCTCCTCCTC CTCATCTTCT 
     3421 GTAACATGAG GAGGCCGGCC CACGCAGACA TCAAGACGGG CTACCTGTCC ATCATCATGG 
     3481 ACCCCGGGGA GGTGCCTCTG GAGGAGCAAT GCGAATACCT GTCCTACGAT GCCAGCCAGT 
     3541 GGGAATTCCC CCGAGAGCGG CTGCACCTGG GGAGAGTGCT CGGCTACGGC GCCTTCGGGA 
     3601 AGGTGGTGGA AGCCTCCGCT TTCGGCATCC ACAAGGGCAG CAGCTGTGAC ACCGTGGCCG 
     3661 TGAAAATGCT GAAAGAGGGC GCCACGGCCA GCGAGCACCG CGCGCTGATG TCGGAGCTCA 
     3721 AGATCCTCAT TCACATCGGC AACCACCTCA ACGTGGTCAA CCTCCTCGGG GCGTGCACCA 
     3781 AGCCGCAGGG CCCCCTCATG GTGATCGTGG AGTTCTGCAA GTACGGCAAC CTCTCCAACT 
     3841 TCCTGCGCGC CAAGCGGGAC GCCTTCAGCC CCTGCGCGGA GAAGTCTCCC GAGCAGCGCG 
     3901 GACGCTTCCG CGCCATGGTG GAGCTCGCCA GGCTGGATCG GAGGCGGCCG GGGAGCAGCG 
     3961 ACAGGGTCCT CTTCGCGCGG TTCTCGAAGA CCGAGGGCGG AGCGAGGCGG GCTTCTCCAG 
     4021 ACCAAGAAGC TGAGGACCTG TGGCTGAGCC CGCTGACCAT GGAAGATCTT GTCTGCTACA 
     4081 GCTTCCAGGT GGCCAGAGGG ATGGAGTTCC TGGCTTCCCG AAAGTGCATC CACAGAGACC 
     4141 TGGCTGCTCG GAACATTCTG CTGTCGGAAA GCGACGTGGT GAAGATCTGT GACTTTGGCC 
     4201 TTGCCCGGGA CATCTACAAA GACCCTGACT ACGTCCGCAA GGGCAGTGCC CGGCTGCCCC 
     4261 TGAAGTGGAT GGCCCCTGAA AGCATCTTCG ACAAGGTGTA CACCACGCAG AGTGACGTGT 
     4321 GGTCCTTTGG GGTGCTTCTC TGGGAGATCT TCTCTCTGGG GGCCTCCCCG TACCCTGGGG 
     4381 TGCAGATCAA TGAGGAGTTC TGCCAGCGGC TGAGAGACGG CACAAGGATG AGGGCCCCGG 
     4441 AGCTGGCCAC TCCCGCCATA CGCCGCATCA TGCTGAACTG CTGGTCCGGA GACCCCAAGG 
     4501 CGAGACCTGC ATTCTCGGAG CTGGTGGAGA TCCTGGGGGA CCTGCTCCAG GGCAGGGGCC 
     4561 TGCAAGAGGA AGAGGAGGTC TGCATGGCCC CGCGCAGCTC TCAGAGCTCA GAAGAGGGCA 
     4621 GCTTCTCGCA GGTGTCCACC ATGGCCCTAC ACATCGCCCA GGCTGACGCT GAGGACAGCC 
     4681 CGCCAAGCCT GCAGCGCCAC AGCCTGGCCG CCAGGTATTA CAACTGGGTG TCCTTTCCCG 
     4741 GGTGCCTGGC CAGAGGGGCT GAGACCCGTG GTTCCTCCAG GATGAAGACA TTTGAGGAAT 
     4801 TCCCCATGAC CCCAACGACC TACAAAGGCT CTGTGGACAA CCAGACAGAC AGTGGGATGG 
     4861 TGCTGGCCTC GGAGGAGTTT GAGCAGATAG AGAGCAGGCA TAGACAAGAA AGCGGCTTCA 
     4921 GCTGTAAAGG ACCTGGCCAG AATGTGGCTG TGACCAGGGC ACACCCTGAC TCCCAAGGGA 
     4981 GGCGGCGGCG GCCTGAGCGG GGGGCCCGAG GAGGCCAGGT GTTTTACAAC AGCGAGTATG 
     5041 GGGAGCTGTC GGAGCCAAGC GAGGAGGACC ACTGCTCCCC GTCTGCCCGC GTGACTTTCT 
     5101 TCACAGACAA CAGCTACACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5161 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5221 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5281 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5341 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5401 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5461 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5521 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5581 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5641 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5701 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5761 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5821 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5881 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5941 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6001 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6061 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6121 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6181 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6241 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6301 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6361 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6421 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6481 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6541 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6601 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6661 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6721 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6781 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6841 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6901 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6961 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7021 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7081 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7141 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7201 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7261 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7321 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7381 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7441 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7501 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7561 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7621 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7681 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7741 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7801 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7861 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7921 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7981 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8041 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8101 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8161 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8221 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8281 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8341 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8401 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8461 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8521 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8581 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8641 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8701 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8761 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8821 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8881 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8941 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//