LOCUS       RC214199                8580 bp    DNA     circular  SYN 
DEFINITION  COPA (Myc-DDK-tagged)-Human coatomer protein complex, subunit alpha (COPA), transcript variant 1
ACCESSION   NM_001098398
VERSION     NM_001098398.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4737..4828 
                     /label="Myc-DDK"  
     misc_feature    4858..5443 
                     /label="PolyA signal"  
     misc_feature    4915..4934 
                     /label="Restriction Enzyme"  
     misc_feature    5719..6391 
                     /label="ColE1"  
     misc_feature    6821..7615 
                     /label="Kan/Neo"  
     misc_feature    7650..7952 
                     /label="SV40 ori"  
     misc_feature    8038..8515 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4719..4725 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4719 
                     /label="COPA"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTAACCAAA TTCGAGACCA AGAGCGCGCG GGTCAAAGGG CTCAGCTTTC 
     1081 ACCCCAAAAG ACCTTGGATC CTGACTAGTT TACATAATGG GGTCATCCAG TTATGGGACT 
     1141 ATCGGATGTG CACTCTCATT GACAAGTTTG ATGAACATGA TGGTCCAGTG CGAGGCATTG 
     1201 ACTTCCATAA GCAGCAGCCA CTGTTCGTCT CTGGAGGAGA TGACTATAAG ATTAAGGTTT 
     1261 GGAATTACAA GCTTCGGCGC TGTCTTTTCA CATTGCTTGG GCACTTAGAT TATATTCGCA 
     1321 CCACGTTTTT TCATCATGAA TATCCCTGGA TTCTGAGTGC CTCCGATGAT CAGACCATCC 
     1381 GAGTGTGGAA TTGGCAATCT AGAACCTGTG TTTGTGTGTT AACAGGGCAC AACCATTATG 
     1441 TGATGTGTGC TCAGTTCCAC CCCACAGAAG ACTTGGTAGT ATCAGCCAGC CTGGACCAGA 
     1501 CTGTGCGCGT TTGGGATATT TCTGGTCTGA GGAAAAAAAA CCTGTCCCCT GGTGCGGTGG 
     1561 AATCGGATGT GAGAGGAATA ACTGGGGTTG ATCTATTTGG AACTACAGAT GCAGTGGTGA 
     1621 AGCATGTACT AGAGGGTCAC GATCGTGGAG TAAACTGGGC TGCCTTCCAC CCCACTATGC 
     1681 CCCTTATTGT ATCTGGGGCA GATGATCGTC AAGTGAAGAT CTGGCGCATG AATGAATCAA 
     1741 AGGCATGGGA GGTTGATACC TGCCGGGGCC ATTACAACAA TGTATCTTGT GCCGTCTTCC 
     1801 ACCCTCGCCA AGAGTTGATC CTCAGCAATT CTGAGGACAA GAGTATTCGA GTCTGGGATA 
     1861 TGTCTAAGCG GACTGGGGTT CAGACTTTCC GCAGAGACCA TGATCGTTTC TGGGTCCTAG 
     1921 CTGCTCACCC TAACCTTAAC CTCTTTGCAG CAGGCCATGA TGGTGGTATG ATTGTGTTTA 
     1981 AGCTGGAACG GGAACGGCCA GCCTATGCTG TTCATGGCAA TATGCTACAC TATGTCAAGG 
     2041 ACCGATTCTT ACGACAGCTG GATTTCAACA GCTCCAAAGA TGTAGCTGTG ATGCAGTTGC 
     2101 GGAGTGGTTC CAAGTTTCCA GTATTCAATA TGTCATACAA TCCAGCAGAA AATGCAGTCC 
     2161 TGCTTTGTAC AAGAGCTAGC AATCTAGAGA ATAGTACCTA TGACCTGTAC ACCATCCCTA 
     2221 AAGATGCTGA CTCCCAGAAT CCTGATGCGC CTGAAGGGAA ACGATCCTCA GGCCTGACAG 
     2281 CCGTTTGGGT CGCTCGAAAT CGGTTTGCTG TCCTAGATCG GATGCATTCG CTTCTGATCA 
     2341 AGAATCTGAA GAATGAGATC ACCAAAAAGG TACAGGTGCC CAACTGTGAT GAGATCTTCT 
     2401 ATGCTGGCAC AGGCAATCTC CTGCTTCGAG ATGCGGACTC TATCACACTC TTTGACGTAC 
     2461 AGCAGAAGCG GACTCTGGCA TCTGTGAAGA TTTCTAAAGT GAAATACGTT ATCTGGTCAG 
     2521 CAGACATGTC ACATGTAGCA CTACTAGCCA AACACGAACA CTCATGCCCT TTGCCTCTTA 
     2581 CAGCCATTGT GATCTGTAAC CGCAAACTGG ATGCTTTATG TAACATTCAT GAGAACATTC 
     2641 GTGTCAAGAG TGGGGCCTGG GATGAGAGTG GGGTATTTAT CTATACCACA AGCAACCACA 
     2701 TCAAATATGC TGTCACCACT GGGGACCACG GGATCATTCG AACTCTGGAT TTACCCATCT 
     2761 ATGTCACACG GGTGAAGGGC AACAATGTAT ACTGCCTAGA CAGGGAGTGT CGTCCCCGGG 
     2821 TACTCACCAT TGATCCCACT GAGTTCAAAT TCAAGCTGGC CCTGATCAAC AGAAAATATG 
     2881 ATGAGGTACT GCACATGGTG AGGAATGCCA AACTAGTTGG CCAGTCTATT ATTGCTTATC 
     2941 TCCAGAAGAA GGGCTATCCT GAAGTGGCAC TGCATTTTGT CAAGGATGAG AAAACTCGCT 
     3001 TTAGTCTGGC ACTGGAGTGT GGAAACATTG AGATTGCTCT GGAAGCAGCC AAAGCACTGG 
     3061 ATGACAAGAA CTGCTGGGAA AAGCTGGGAG AAGTGGCCCT GCTGCAGGGG AACCACCAGA 
     3121 TTGTGGAAAT GTGCTATCAG CGTACCAAAA ACTTTGACAA ACTTTCCTTC CTGTATCTTA 
     3181 TCACTGGCAA CTTAGAAAAA CTTCGCAAGA TGATGAAGAT TGCTGAGATC AGAAAGGACA 
     3241 TGAGTGGCCA CTATCAGAAT GCCCTATACC TGGGTGATGT GTCAGAGCGT GTGCGGATCC 
     3301 TGAAGAACTG TGGACAGAAG TCCCTGGCCT ATCTCACAGC TGCTACCCAT GGCTTAGATG 
     3361 AAGAAGCTGA GAGCCTAAAG GAGACATTTG ACCCAGAGAA GGAGACAATC CCAGACATTG 
     3421 ACCCTAATGC CAAGCTGCTC CAGCCACCTG CACCTATCAT GCCATTGGAT ACCAATTGGC 
     3481 CTTTATTGAC TGTATCCAAA GGATTTTTTG AAGGCACCAT TGCCAGCAAA GGGAAGGGAG 
     3541 GAGCACTGGC TGCTGACATT GACATTGACA CTGTTGGTAC AGAGGGCTGG GGAGAGGATG 
     3601 CAGAGCTGCA GTTGGATGAA GATGGGTTTG TGGAGGCTAC AGAAGGTTTG GGGGATGATG 
     3661 CTCTTGGCAA GGGACAGGAA GAAGGAGGTG GCTGGGATGT AGAAGAAGAT CTGGAGCTCC 
     3721 CTCCTGAGCT GGATATATCC CCTGGGGCAG CTGGTGGGGC TGAAGATGGT TTCTTTGTGC 
     3781 CCCCAACCAA GGGAACAAGT CCAACTCAGA TCTGGTGTAA TAACTCTCAG CTTCCAGTTG 
     3841 ATCACATCCT GGCAGGCTCT TTCGAAACAG CCATGCGGCT CCTTCATGAC CAAGTAGGGG 
     3901 TAATCCAGTT TGGCCCCTAC AAGCAACTGT TCCTACAGAC ATACGCCCGA GGCCGCACAA 
     3961 CCTATCAGGC TCTGCCCTGC CTACCCTCCA TGTATGGCTA TCCTAATCGC AACTGGAAGG 
     4021 ATGCAGGGCT GAAGAATGGT GTACCAGCTG TGGGCCTGAA GCTTAATGAC CTCATCCAAC 
     4081 GGTTGCAGCT GTGCTACCAG CTCACCACAG TTGGCAAATT TGAGGAGGCT GTGGAAAAAT 
     4141 TCCGTTCCAT CCTTCTCAGT GTGCCACTTC TTGTTGTGGA CAATAAACAA GAGATTGCAG 
     4201 AGGCCCAGCA GCTCATCACC ATTTGCCGTG AGTACATTGT GGGTTTGTCC GTGGAGACAG 
     4261 AAAGGAAGAA GCTGCCCAAA GAGACTCTAG AACAGCAGAA GCGCATCTGT GAGATGGCAG 
     4321 CCTATTTCAC CCACTCAAAC CTGCAGCCTG TGCACATGAT CCTGGTGCTG CGTACAGCCC 
     4381 TCAATCTGTT CTTCAAGCTC AAGAACTTCA AGACAGCTGC CACCTTTGCT CGGCGCCTAC 
     4441 TAGAACTCGG GCCCAAGCCT GAGGTGGCCC AACAGACCCG AAAAATCCTG TCTGCCTGTG 
     4501 AGAAGAATCC CACAGATGCC TACCAGCTCA ATTATGACAT GCACAACCCC TTTGACATTT 
     4561 GTGCTGCATC ATATCGGCCC ATCTACCGTG GAAAGCCAGT AGAAAAGTGT CCACTCAGTG 
     4621 GGGCCTGCTA TTCCCCTGAG TTCAAAGGTC AAATCTGCAG GGTCACCACA GTGACAGAGA 
     4681 TTGGCAAAGA TGTGATTGGT TTAAGGATCA GTCCTCTGCA GTTTCGCACG CGTACGCGGC 
     4741 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4801 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4861 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4921 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4981 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5041 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5101 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5161 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5221 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5281 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5341 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5401 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5461 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5521 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5581 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5641 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5701 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5761 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5821 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5881 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5941 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6001 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6061 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6121 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6181 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6241 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6301 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6361 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6421 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6481 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6541 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6601 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6661 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6721 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6781 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6841 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6901 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6961 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7021 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7081 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7141 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7201 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7261 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7321 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7381 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7441 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7501 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7561 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7621 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7681 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7741 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7801 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7861 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7921 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7981 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8041 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8101 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8161 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8221 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8281 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8341 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8401 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8461 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8521 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//