LOCUS       RC214156L2                8790 bp    DNA     circular  SYN 
DEFINITION  BEST1 (Myc-DDK-tagged)-Human bestrophin 1 (BEST1), transcript variant 1
ACCESSION   NM_004183
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7081..7261 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    7989..8648 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7309..7928 
                     /label="ColE1"  
     misc_feature    5956..5976 
                     /label="LR50"  
     misc_feature    5096..5821 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    5965..6556 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5075..5081 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5075 
                     /label="BEST1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GACCATCACT TACACAAGCC AAGTGGCTAA 
     3361 TGCCCGCTTA GGCTCCTTCT CCCGCCTGCT GCTGTGCTGG CGGGGCAGCA TCTACAAGCT 
     3421 GCTATATGGC GAGTTCTTAA TCTTCCTGCT CTGCTACTAC ATCATCCGCT TTATTTATAG 
     3481 GCTGGCCCTC ACGGAAGAAC AACAGCTGAT GTTTGAGAAA CTGACTCTGT ATTGCGACAG 
     3541 CTACATCCAG CTCATCCCCA TTTCCTTCGT GCTGGGCTTC TACGTGACGC TGGTCGTGAC 
     3601 CCGCTGGTGG AACCAGTACG AGAACCTGCC GTGGCCCGAC CGCCTCATGA GCCTGGTGTC 
     3661 GGGCTTCGTC GAAGGCAAGG ACGAGCAAGG CCGGCTGCTG CGGCGCACGC TCATCCGCTA 
     3721 CGCCAACCTG GGCAACGTGC TCATCCTGCG CAGCGTCAGC ACCGCAGTCT ACAAGCGCTT 
     3781 CCCCAGCGCC CAGCACCTGG TGCAAGCAGG CTTTATGACT CCGGCAGAAC ACAAGCAGTT 
     3841 GGAGAAACTG AGCCTACCAC ACAACATGTT CTGGGTGCCC TGGGTGTGGT TTGCCAACCT 
     3901 GTCAATGAAG GCGTGGCTTG GAGGTCGAAT CCGGGACCCT ATCCTGCTCC AGAGCCTGCT 
     3961 GAACGAGATG AACACCTTGC GTACTCAGTG TGGACACCTG TATGCCTACG ACTGGATTAG 
     4021 TATCCCACTG GTGTATACAC AGGTGGTGAC TGTGGCGGTG TACAGCTTCT TCCTGACTTG 
     4081 TCTAGTTGGG CGGCAGTTTC TGAACCCAGC CAAGGCCTAC CCTGGCCATG AGCTGGACCT 
     4141 CGTTGTGCCC GTCTTCACGT TCCTGCAGTT CTTCTTCTAT GTTGGCTGGC TGAAGGTGGC 
     4201 AGAGCAGCTC ATCAACCCCT TTGGAGAGGA TGATGATGAT TTTGAGACCA ACTGGATTGT 
     4261 CGACAGGAAT TTGCAGGTGT CCCTGTTGGC TGTGGATGAG ATGCACCAGG ACCTGCCTCG 
     4321 GATGGAGCCG GACATGTACT GGAATAAGCC CGAGCCACAG CCCCCCTACA CAGCTGCTTC 
     4381 CGCCCAGTTC CGTCGAGCCT CCTTTATGGG CTCCACCTTC AACATCAGCC TGAACAAAGA 
     4441 GGAGATGGAG TTCCAGCCCA ATCAGGAGGA CGAGGAGGAT GCTCACGCTG GCATCATTGG 
     4501 CCGCTTCCTA GGCCTGCAGT CCCATGATCA CCATCCTCCC AGGGCAAACT CAAGGACCAA 
     4561 ACTACTGTGG CCCAAGAGGG AATCCCTTCT CCACGAGGGC CTGCCCAAAA ACCACAAGGC 
     4621 AGCCAAACAG AACGTTAGGG GCCAGGAAGA CAACAAGGCC TGGAAGCTTA AGGCTGTGGA 
     4681 CGCCTTCAAG TCTGCCCCAC TGTATCAGAG GCCAGGCTAC TACAGTGCCC CACAGACGCC 
     4741 CCTCAGCCCC ACTCCCATGT TCTTCCCCCT AGAACCATCA GCGCCGTCAA AGCTTCACAG 
     4801 TGTCACAGGC ATAGACACCA AAGACAAAAG CTTAAAGACT GTGAGTTCTG GGGCCAAGAA 
     4861 AAGTTTTGAA TTGCTCTCAG AGAGCGATGG GGCCTTGATG GAGCACCCAG AAGTATCTCA 
     4921 AGTGAGGAGG AAAACTGTGG AGTTTAACCT GACGGATATG CCAGAGATCC CCGAAAATCA 
     4981 CCTCAAAGAA CCTTTGGAAC AATCACCAAC CAACATACAC ACTACACTCA AAGATCACAT 
     5041 GGATCCTTAT TGGGCCTTGG AAAACAGGGA TGAAGCACAT TCCACGCGTA CGCGGCCGCT 
     5101 CGAGATGAGC GGGGGCGAGG AGCTGTTCGC CGGCATCGTG CCCGTGCTGA TCGAGCTGGA 
     5161 CGGCGACGTG CACGGCCACA AGTTCAGCGT GCGCGGCGAG GGCGAGGGCG ACGCCGACTA 
     5221 CGGCAAGCTG GAGATCAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC 
     5281 CCTGGTGACC ACCCTCTGCT ACGGCATCCA GTGCTTCGCC CGCTACCCCG AGCACATGAA 
     5341 GATGAACGAC TTCTTCAAGA GCGCCATGCC CGAGGGCTAC ATCCAGGAGC GCACCATCCA 
     5401 GTTCCAGGAC GACGGCAAGT ACAAGACCCG CGGCGAGGTG AAGTTCGAGG GCGACACCCT 
     5461 GGTGAACCGC ATCGAGCTGA AGGGCAAGGA CTTCAAGGAG GACGGCAACA TCCTGGGCCA 
     5521 CAAGCTGGAG TACAGCTTCA ACAGCCACAA CGTGTACATC CGCCCCGACA AGGCCAACAA 
     5581 CGGCCTGGAG GCTAACTTCA AGACCCGCCA CAACATCGAG GGCGGCGGCG TGCAGCTGGC 
     5641 CGACCACTAC CAGACCAACG TGCCCCTGGG CGACGGCCCC GTGCTGATCC CCATCAACCA 
     5701 CTACCTGAGC ACTCAGACCA AGATCAGCAA GGACCGCAAC GAGGCCCGCG ACCACATGGT 
     5761 GCTCCTGGAG TCCTTCAGCG CCTGCTGCCA CACCCACGGC ATGGACGAGC TGTACAGGTC 
     5821 CGGACTCAGA TAAGTTTAAA CGGCCGGCCG CGGTCTGTAC AAGTAGGATT CGTCGAGGGA 
     5881 CCTAATAACT TCGTATAGCA TACATTATAC GAAGTTATAC ATGTTTAAGG GTTCCGGTTC 
     5941 CACTAGGTAC AATTCGATAT CAAGCTTATC GATAATCAAC CTCTGGATTA CAAAATTTGT 
     6001 GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT 
     6061 TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT 
     6121 AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG 
     6181 GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG 
     6241 CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC 
     6301 TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG 
     6361 TCGGGGAAAT CATCGTCCTT TCCTTGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC 
     6421 GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC 
     6481 CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC GAGTCGGATC 
     6541 TCCCTTTGGG CCGCCTCCCC GCATCGATAC CGTCGACCTC GATCGAGACC TAGAAAAACA 
     6601 TGGAGCAATC ACAAGTAGCA ATACAGCAGC TACCAATGCT GATTGTGCCT GGCTAGAAGC 
     6661 ACAAGAGGAG GAGGAGGTGG GTTTTCCAGT CACACCTCAG GTACCTTTAA GACCAATGAC 
     6721 TTACAAGGCA GCTGTAGATC TTAGCCACTT TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT 
     6781 AATTCACTCC CAACGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA CACAAGGCTA 
     6841 CTTCCCTGAT TGGCAGAACT ACACACCAGG GCCAGGGATC AGATATCCAC TGACCTTTGG 
     6901 ATGGTGCTAC AAGCTAGTAC CAGTTGAGCA AGAGAAGGTA GAAGAAGCCA ATGAAGGAGA 
     6961 GAACACCCGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG AGAGAGAAGT 
     7021 ATTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC ATGGCCCGAG AGCTGCATCC 
     7081 GGACTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT CTGGCTAACT 
     7141 AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC 
     7201 CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA 
     7261 AATCTCTAGC AGCATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     7321 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     7381 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     7441 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     7501 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     7561 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     7621 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     7681 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     7741 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     7801 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     7861 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     7921 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     7981 AGGGATTTTG GTCATGATTA CGCCCCGCCC TGCCACTCAT CGCAGTACTG TTGTAATTCA 
     8041 TTAAGCATTC TGCCGACATG GAAGCCATCA CAAACGGCAT GATGAACCTG AATCGCCAGC 
     8101 GGCATCAGCA CCTTGTCGCC TTGCGTATAA TATTTGCCCA TGGTGAAAAC GGGGGCGAAG 
     8161 AAGTTGTCCA TATTGGCCAC GTTTAAATCA AAACTGGTGA AACTCACCCA GGGATTGGCT 
     8221 GAGACGAAAA ACATATTCTC AATAAACCCT TTAGGGAAAT AGGCCAGGTT TTCACCGTAA 
     8281 CACGCCACAT CTTGCGAATA TATGTGTAGA AACTGCCGGA AATCGTCGTG GTATTCACTC 
     8341 CAGAGCGATG AAAACGTTTC AGTTTGCTCA TGGAAAACGG TGTAACAAGG GTGAACACTA 
     8401 TCCCATATCA CCAGCTCACC GTCTTTCATT GCCATACGGA ACTCCGGATG AGCATTCATC 
     8461 AGGCGGGCAA GAATGTGAAT AAAGGCCGGA TAAAACTTGT GCTTATTTTT CTTTACGGTC 
     8521 TTTAAAAAGG CCGTAATATC CAGCTGAACG GTCTGGTTAT AGGTACATTG AGCAACTGAC 
     8581 TGAAATGCCT CAAAATGTTC TTTACGATGC CATTGGGATA TATCAACGGT GGTATATCCA 
     8641 GTGATTTTTT TCTCCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8701 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8761 CGCACATTTC CCCGAAAAGT GCCACCTGAC 
//