LOCUS       RC214125                7296 bp    DNA     circular  SYN 
DEFINITION  PIK3AP1 (Myc-DDK-tagged)-Human phosphoinositide-3-kinase adaptor protein 1 (PIK3AP1)
ACCESSION   NM_152309
VERSION     NM_152309.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3453..3544 
                     /label="Myc-DDK"  
     misc_feature    3574..4159 
                     /label="PolyA signal"  
     misc_feature    3631..3650 
                     /label="Restriction Enzyme"  
     misc_feature    4435..5107 
                     /label="ColE1"  
     misc_feature    5537..6331 
                     /label="Kan/Neo"  
     misc_feature    6366..6668 
                     /label="SV40 ori"  
     misc_feature    6754..7231 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3435..3441 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3435 
                     /label="PIK3AP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGCCTCA GGGGTGCCCA GAGGATGCGA CATCCTCATC GTCTACAGCC 
     1081 CGGATGCCGA GGAATGGTGC CAGTACCTGC AGACCCTGTT CCTGTCCAGT CGGCAGGTCC 
     1141 GCAGCCAGAA GATACTGACT CACAGGCTGG GCCCCGAGGC CTCCTTCTCG GCAGAGGACC 
     1201 TAAGCCTTTT CCTCAGCACC CGCTGTGTCG TGGTGCTGCT GTCCGCGGAG CTGGTGCAGC 
     1261 ACTTCCACAA GCCCTCCTTG CTGCCCCTGC TGCAGAGAGC TTTCCATCCT CCGCACCGCG 
     1321 TGGTCAGGCT GCTCTGCGGC GTGCGGGACA GCGAGGAGTT CCTAGACTTC TTTCCAGATT 
     1381 GGGCCCATTG GCAGGAGCTC ACCTGTGACG ATGAGCCAGA GACCTACGTG GCAGCTGTGA 
     1441 AAAAAGCCAT TTCCGAAGAT TCTGGCTGTG ACTCAGTCAC TGACACTGAG CCTGAGGACG 
     1501 AGAAGGTTGT TTCCTACTCG AAGCAGCAGA ACCTGCCGAC GGTGACTTCA CCTGGGAACC 
     1561 TGATGGTGGT GCAGCCGGAC CGCATTCGCT GTGGGGCAGA AACCACTGTC TATGTTATTG 
     1621 TGAGATGTAA GCTGGATGAC AGGGTGGCGA CAGAAGCAGA GTTTTCTCCT GAGGATTCTC 
     1681 CCTCTGTAAG GATGGAAGCC AAGGTGGAGA ATGAGTACAC CATTTCAGTG AAGGCTCCCA 
     1741 ACCTTTCATC TGGGAACGTT TCTCTGAAGA TATATTCTGG AGACTTAGTG GTGTGTGAAA 
     1801 CCGTTATCAG CTATTATACT GACATGGAAG AAATTGGGAA TTTATTGTCC AATGCCGCGA 
     1861 ATCCTGTGGA ATTCATGTGT CAGGCCTTTA AAATTGTGCC CTACAACACA GAGACCCTTG 
     1921 ATAAACTGCT AACCGAATCC CTGAAGAACA ATATCCCTGC AAGCGGACTG CACCTCTTTG 
     1981 GAATCAACCA GCTGGAAGAA GAAGATATGA TGACAAATCA GAGGGATGAA GAGCTGCCCA 
     2041 CCCTGTTGCA TTTTGCTGCG AAGTATGGAC TGAAGAACCT CACTGCCTTG TTGCTCACCT 
     2101 GCCCAGGAGC CCTGCAGGCG TACAGCGTGG CCAACAAGCA TGGCCACTAC CCCAACACCA 
     2161 TCGCTGAGAA ACACGGCTTC AGGGACCTGC GGCAGTTCAT CGACGAGTAT GTGGAAACGG 
     2221 TGGACATGCT CAAGAGTCAC ATTAAAGAGG AACTGATGCA CGGGGAGGAG GCTGATGCTG 
     2281 TGTACGAGTC CATGGCCCAC CTTTCCACAG ACCTGCTTAT GAAATGCTCG CTCAACCCCG 
     2341 GCTGTGACGA GGATCTCTAT GAGTCCATGG CTGCCTTTGT CCCAGCTGCC ACTGAAGACC 
     2401 TCTATGTTGA AATGCTTCAG GCCAGTACAT CTAACCCAAT CCCTGGAGAT GGTTTCTCTC 
     2461 GGGCCACTAA GGACTCTATG ATCCGCAAGT TTTTAGAAGG CAACAGCATG GGAATGACCA 
     2521 ATCTGGAGAG AGATCAGTGC CATCTTGGTC AGGAAGAAGA TGTTTATCAC ACGGTGGATG 
     2581 ACGATGAGGC CTTTTCTGTG GACTTGGCCA GCAGGCCCCC TGTCCCAGTG CCCAGACCAG 
     2641 AGACCACTGC TCCTGGTGCT CACCAGCTGC CTGACAACGA ACCATACATT TTTAAAGTTT 
     2701 TTGCAGAAAA AAGTCAAGAG CGGCCTGGGA ATTTCTACGT TTCCTCAGAG AGCATCAGGA 
     2761 AAGGGCCGCC CGTCAGACCA TGGAGGGACA GGCCCCAGTC AAGTATATAT GACCCTTTTG 
     2821 CGGGAATGAA AACGCCAGGC CAGCGGCAGC TTATCACCCT CCAGGAGCAG GTGAAGCTGG 
     2881 GCATTGTCAA CGTGGATGAG GCTGTGCTCC ACTTCAAAGA GTGGCAGCTC AACCAGAAGA 
     2941 AACGATCGGA GTCCTTTCGT TTCCAGCAGG AAAATCTTAA ACGGCTAAGA GACAGCATCA 
     3001 CCCGAAGACA GAGAGAGAAG CAAAAATCAG GAAAGCAGAC AGACTTGGAG ATCACGGTCC 
     3061 CAATTCGGCA CTCACAGCAC CTGCCTGCAA AAGTGGAGTT TGGAGTCTAT GAGAGTGGCC 
     3121 CCAGGAAAAG TGTCATTCCC CCTAGGACGG AGCTGAGACG AGGAGACTGG AAAACAGACA 
     3181 GCACCTCCAG CACAGCAAGT AGCACAAGTA ACCGCTCCAG CACCCGGAGC CTCCTCAGTG 
     3241 TGAGCAGCGG GATGGAAGGG GACAACGAGG ATAATGAAGT CCCTGAGGTT ACCAGAAGTC 
     3301 GCAGTCCAGG CCCCCCACAA GTGGATGGGA CACCCACCAT GTCCCTCGAG AGACCCCCCA 
     3361 GGGTGCCTCC GAGAGCTGCC TCACAGAGGC CTCCGACCAG GGAGACCTTC CATCCTCCTC 
     3421 CACCTGTTCC ACCCAGAGGA CGCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3481 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3541 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3601 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3661 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3721 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3781 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3841 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     3901 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     3961 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4021 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4081 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4141 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4201 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4261 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4321 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4381 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4441 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4501 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4561 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4621 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4681 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4741 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4801 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4861 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     4921 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     4981 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5041 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5101 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5161 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5221 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5281 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5341 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5401 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5461 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5521 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5581 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5641 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5701 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5761 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5821 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     5881 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     5941 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6001 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6061 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6121 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6181 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6241 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6301 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6361 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6421 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6481 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6541 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6601 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6661 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6721 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6781 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6841 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     6901 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     6961 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7021 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7081 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7141 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7201 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7261 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//