LOCUS       RC214121L1                9495 bp    DNA     circular  SYN 
DEFINITION  DSG4 (Myc-DDK-tagged)-Human desmoglein 4 (DSG4), transcript variant 2
ACCESSION   NM_177986
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7786..7966 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8694..9353 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    8125..8693 
                     /label="ColE1"  
     misc_feature    6661..6681 
                     /label="LR50 primer"  
     misc_feature    6458..6529 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6670..7261 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6440..6446 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6440 
                     /label="DSG4"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGATTGGCTC TTCTTCAGAA ACATTTGCCT 
     3361 TTTGATCATT CTAATGGTGG TGATGGAAGT AAACAGTGAA TTTATTGTTG AGGTGAAGGA 
     3421 ATTTGACATT GAAAATGGCA CTACAAAATG GCAAACAGTC AGAAGACAAA AGCGGGAGTG 
     3481 GATCAAGTTT GCCGCAGCCT GTCGAGAAGG AGAGGACAAC TCGAAGAGGA ACCCCATTGC 
     3541 CAAAATTCGA TCAGACTGCG AATCGAACCA GAAGATAACA TACCGGATTT CTGGAGTAGG 
     3601 GATTGATCGA CCACCATATG GGGTATTCAC CATTAATCCT CGCACTGGGG AAATTAACAT 
     3661 CACTTCAGTG GTAGACAGAG AAATAACTCC ACTTTTCTTG ATCTATTGCC GGGCTCTGAA 
     3721 TTCACGGGGT GAAGATTTAG AAAGGCCTCT TGAGCTTAGA GTCAAAGTTA TGGACATAAA 
     3781 TGATAACGCT CCAGTCTTTT CGCAAAGTGT ATACACAGCC AGCATTGAAG AAAATAGTGA 
     3841 TGCCAATACA TTGGTAGTAA AGTTATGTGC CACAGATGCA GATGAAGAAA ATCATCTGAA 
     3901 TTCTAAAATT GCCTACAAGA TCGTCTCTCA GGAGCCATCA GGTGCACCCA TGTTCATTCT 
     3961 GAATAGGTAC ACTGGAGAAG TCTGCACCAT GTCCAGTTTC TTGGACAGAG AGCAACACAG 
     4021 TATGTACAAC CTGGTTGTGA GAGGCTCAGA TCGGGATGGA GCTGCAGATG GACTGTCTTC 
     4081 TGAGTGTGAC TGTAGAATCA AGGTTTTAGA CGTCAACGAT AATTTCCCCA CCTTAGAGAA 
     4141 AACTTCATAC TCAGCCAGTA TTGAAGAGAA TTGTTTAAGT TCGGAACTGA TACGATTACA 
     4201 AGCAATTGAT CTTGATGAAG AAGGCACTGA TAACTGGTTG GCTCAATATT TAATTCTCTC 
     4261 TGGAAATGAT GGGAATTGGT TCGATATTCA AACAGATCCA CAAACCAATG AAGGCATTTT 
     4321 GAAAGTTGTC AAGATGCTGG ATTATGAACA AGCACCTAAC ATTCAGCTTA GTATCGGAGT 
     4381 TAAAAACCAA GCTGATTTTC ACTACTCCGT TGCTTCTCAA TTCCAAATGC ACCCAACCCC 
     4441 TGTGAGAATT CAAGTTGTTG ATGTGAGAGA AGGACCTGCA TTTCATCCAA GTACTATGGC 
     4501 TTTTAGTGTG CGGGAAGGAA TAAAAGGAAG TTCCTTATTG AATTATGTGC TTGGCACATA 
     4561 TACAGCCATA GATTTGGACA CAGGAAACCC TGCAACAGAT GTCAGATATA TCATAGGGCA 
     4621 TGATGCAGGC AGCTGGTTAA AAATTGATTC AAGAACTGGT GAGATACAAT TTTCTAGAGA 
     4681 ATTTGATAAG AAGTCAAAAT ATATTATCAA TGGGATATAC ACAGCAGAGA TCCTGGCTAT 
     4741 AGATGATGGC TCTGGAAAAA CAGCTACAGG AACCATATGT ATTGAGGTTC CTGATATCAA 
     4801 TGATTATTGT CCAAACATTT TTCCTGAAAG AAGAACCATC TGCATTGACT CTCCATCAGT 
     4861 CCTTATCTCT GTTAATGAAC ATTCTTATGG GTCTCCGTTT ACTTTCTGTG TTGTTGATGA 
     4921 GCCACCAGGA ATAGCTGACA TGTGGGATGT CAGATCAACA AATGCTACCT CGGCAATCCT 
     4981 TACGGCTAAG CAGGTTTTAT CTCCAGGATT TTATGAAATC CCAATCCTGG TGAAGGACAG 
     5041 CTATAACAGA GCATGTGAAT TGGCACAAAT GGTGCAGTTA TATGCCTGTG ATTGCGATGA 
     5101 CAACCACATG TGCCTGGACT CTGGTGCCGC GGGCATCTAC ACAGAGGACA TAACTGGTGA 
     5161 CACGTATGGG CCTGTCACTG AAGACCAAGC TGGAGTTTCA AATGTTGGTC TTGGACCAGC 
     5221 AGGGATTGGC ATGATGGTTC TGGGCATCCT GCTACTGCTT TTGGCTCCAC TCTTGCTGCT 
     5281 CCTGTGTTGC TGCAAACAGA GACAGCCAGA AGGCCTGGGA ACAAGATTTG CTCCTGTGCC 
     5341 TGAGGGCGGA GAAGGAGTGA TGCAGTCTTG GAGAATTGAA GGGGCCCATC CCGAGGACAG 
     5401 GGATGTGTCA AATATATGTG CACCCATGAC AGCCTCAAAT ACCCAGGATC GGATGGATTC 
     5461 CTCTGAAATC TACACCAACA CCTATGCAGC CGGGGGCACG GTGGAAGGAG GTGTATCGGG 
     5521 AGTGGAGCTC AACACAGGTA TGGGGACAGC CGTTGGCCTC ATGGCCGCAG GGGCCGCAGG 
     5581 AGCCTCAGGG GCCGCAAGGA AGAGGAGCTC TACCATGGGA ACCCTGCGGG ACTACGCTGA 
     5641 CGCAGACATC AACATGGCTT TCTTGGACAG CTACTTCTCG GAGAAAGCGT ATGCTTATGC 
     5701 AGATGAAGAT GAAGGTCGAC CAGCCAATGA CTGCTTGCTC ATTTATGACC ACGAGGGAGT 
     5761 CGGGTCTCCC GTAGGCTCTA TTGGTTGTTG CAGTTGGATT GTGGATGACT TAGATGAAAG 
     5821 CTGCATGGAA ACTTTAGATC CAAAATTTAG GACTCTTGCT GAGATCTGCT TAAACACAGA 
     5881 AATTGAACCA TTTCCTTCAC ACCAGGCTTG TATACCAATC AGTACTGACC TCCCTTTGCT 
     5941 CGGACCTAAT TACTTTGTTA ATGAATCTTC AGGATTGACT CCCTCAGAAG TTGAATTCCA 
     6001 AGAAGAAATG GCAGCATCTG AACCCGTGGT CCATGGGGAT ATTATTGTGA CTGAGACTTA 
     6061 CGGTAATGCT GATCCATGTG TGCAACCCAC TACAATTATT TTTGATCCTC AGCTTGCACC 
     6121 CAATGTTGTA GTAACCGAAG CAGTAATGGC ACCTGTCTAT GATATTCAAG GGAATATTTG 
     6181 TGTACCTGCT GAGTTAGCAG ATTACAACAA TGTAATCTAT GCTGAGAGAG TACTGGCTAG 
     6241 TCCTGGTGTG CCTGACATGA GCAATAGTAG CACGACTGAG GGTTGTATGG GACCTGTGAT 
     6301 GAGCGGCAAT ATTTTAGTAG GGCCAGAAAT TCAAGTGATG CAAATGATGA GTCCAGACCT 
     6361 TCCCATAGGC CAAACCGTTG GCTCCACATC CCCAATGACA TCTCGACACA GAGTAACACG 
     6421 ATACAGTAAC ATACATTACA CCCAACAGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT 
     6481 CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT 
     6541 TTAAACGGCC GGCCGCGGTC TGTACAAGTA GGATTCGTCG AGGGACCTAA TAACTTCGTA 
     6601 TAGCATACAT TATACGAAGT TATACATGTT TAAGGGTTCC GGTTCCACTA GGTACAATTC 
     6661 GATATCAAGC TTATCGATAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT 
     6721 ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT 
     6781 CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG 
     6841 TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT 
     6901 GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT 
     6961 TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC 
     7021 TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAATCATCG 
     7081 TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC 
     7141 TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG 
     7201 CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC 
     7261 TCCCCGCATC GATACCGTCG ACCTCGATCG AGACCTAGAA AAACATGGAG CAATCACAAG 
     7321 TAGCAATACA GCAGCTACCA ATGCTGATTG TGCCTGGCTA GAAGCACAAG AGGAGGAGGA 
     7381 GGTGGGTTTT CCAGTCACAC CTCAGGTACC TTTAAGACCA ATGACTTACA AGGCAGCTGT 
     7441 AGATCTTAGC CACTTTTTAA AAGAAAAGGG GGGACTGGAA GGGCTAATTC ACTCCCAACG 
     7501 AAGACAAGAT ATCCTTGATC TGTGGATCTA CCACACACAA GGCTACTTCC CTGATTGGCA 
     7561 GAACTACACA CCAGGGCCAG GGATCAGATA TCCACTGACC TTTGGATGGT GCTACAAGCT 
     7621 AGTACCAGTT GAGCAAGAGA AGGTAGAAGA AGCCAATGAA GGAGAGAACA CCCGCTTGTT 
     7681 ACACCCTGTG AGCCTGCATG GGATGGATGA CCCGGAGAGA GAAGTATTAG AGTGGAGGTT 
     7741 TGACAGCCGC CTAGCATTTC ATCACATGGC CCGAGAGCTG CATCCGGACT GTACTGGGTC 
     7801 TCTCTGGTTA GACCAGATCT GAGCCTGGGA GCTCTCTGGC TAACTAGGGA ACCCACTGCT 
     7861 TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA 
     7921 CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG TGGAAAATCT CTAGCAGCAT 
     7981 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     8041 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     8101 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     8161 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     8221 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     8281 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     8341 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     8401 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     8461 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     8521 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     8581 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     8641 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     8701 GATTACGCCC CGCCCTGCCA CTCATCGCAG TACTGTTGTA ATTCATTAAG CATTCTGCCG 
     8761 ACATGGAAGC CATCACAAAC GGCATGATGA ACCTGAATCG CCAGCGGCAT CAGCACCTTG 
     8821 TCGCCTTGCG TATAATATTT GCCCATGGTG AAAACGGGGG CGAAGAAGTT GTCCATATTG 
     8881 GCCACGTTTA AATCAAAACT GGTGAAACTC ACCCAGGGAT TGGCTGAGAC GAAAAACATA 
     8941 TTCTCAATAA ACCCTTTAGG GAAATAGGCC AGGTTTTCAC CGTAACACGC CACATCTTGC 
     9001 GAATATATGT GTAGAAACTG CCGGAAATCG TCGTGGTATT CACTCCAGAG CGATGAAAAC 
     9061 GTTTCAGTTT GCTCATGGAA AACGGTGTAA CAAGGGTGAA CACTATCCCA TATCACCAGC 
     9121 TCACCGTCTT TCATTGCCAT ACGGAACTCC GGATGAGCAT TCATCAGGCG GGCAAGAATG 
     9181 TGAATAAAGG CCGGATAAAA CTTGTGCTTA TTTTTCTTTA CGGTCTTTAA AAAGGCCGTA 
     9241 ATATCCAGCT GAACGGTCTG GTTATAGGTA CATTGAGCAA CTGACTGAAA TGCCTCAAAA 
     9301 TGTTCTTTAC GATGCCATTG GGATATATCA ACGGTGGTAT ATCCAGTGAT TTTTTTCTCC 
     9361 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     9421 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TCCCGCGCAC ATTTCCCCGA 
     9481 AAAGTGCCAC CTGAC
//