LOCUS       RC214121                8001 bp    DNA     circular  SYN 
DEFINITION  DSG4 (Myc-DDK-tagged)-Human desmoglein 4 (DSG4), transcript variant 2
ACCESSION   NM_177986
VERSION     NM_177986.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4158..4249 
                     /label="Myc-DDK"  
     misc_feature    4279..4864 
                     /label="PolyA signal"  
     misc_feature    4336..4355 
                     /label="Restriction Enzyme"  
     misc_feature    5140..5812 
                     /label="ColE1"  
     misc_feature    6242..7036 
                     /label="Kan/Neo"  
     misc_feature    7071..7373 
                     /label="SV40 ori"  
     misc_feature    7459..7936 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4140..4146 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4140 
                     /label="DSG4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATTGGCTC TTCTTCAGAA ACATTTGCCT TTTGATCATT CTAATGGTGG 
     1081 TGATGGAAGT AAACAGTGAA TTTATTGTTG AGGTGAAGGA ATTTGACATT GAAAATGGCA 
     1141 CTACAAAATG GCAAACAGTC AGAAGACAAA AGCGGGAGTG GATCAAGTTT GCCGCAGCCT 
     1201 GTCGAGAAGG AGAGGACAAC TCGAAGAGGA ACCCCATTGC CAAAATTCGA TCAGACTGCG 
     1261 AATCGAACCA GAAGATAACA TACCGGATTT CTGGAGTAGG GATTGATCGA CCACCATATG 
     1321 GGGTATTCAC CATTAATCCT CGCACTGGGG AAATTAACAT CACTTCAGTG GTAGACAGAG 
     1381 AAATAACTCC ACTTTTCTTG ATCTATTGCC GGGCTCTGAA TTCACGGGGT GAAGATTTAG 
     1441 AAAGGCCTCT TGAGCTTAGA GTCAAAGTTA TGGACATAAA TGATAACGCT CCAGTCTTTT 
     1501 CGCAAAGTGT ATACACAGCC AGCATTGAAG AAAATAGTGA TGCCAATACA TTGGTAGTAA 
     1561 AGTTATGTGC CACAGATGCA GATGAAGAAA ATCATCTGAA TTCTAAAATT GCCTACAAGA 
     1621 TCGTCTCTCA GGAGCCATCA GGTGCACCCA TGTTCATTCT GAATAGGTAC ACTGGAGAAG 
     1681 TCTGCACCAT GTCCAGTTTC TTGGACAGAG AGCAACACAG TATGTACAAC CTGGTTGTGA 
     1741 GAGGCTCAGA TCGGGATGGA GCTGCAGATG GACTGTCTTC TGAGTGTGAC TGTAGAATCA 
     1801 AGGTTTTAGA CGTCAACGAT AATTTCCCCA CCTTAGAGAA AACTTCATAC TCAGCCAGTA 
     1861 TTGAAGAGAA TTGTTTAAGT TCGGAACTGA TACGATTACA AGCAATTGAT CTTGATGAAG 
     1921 AAGGCACTGA TAACTGGTTG GCTCAATATT TAATTCTCTC TGGAAATGAT GGGAATTGGT 
     1981 TCGATATTCA AACAGATCCA CAAACCAATG AAGGCATTTT GAAAGTTGTC AAGATGCTGG 
     2041 ATTATGAACA AGCACCTAAC ATTCAGCTTA GTATCGGAGT TAAAAACCAA GCTGATTTTC 
     2101 ACTACTCCGT TGCTTCTCAA TTCCAAATGC ACCCAACCCC TGTGAGAATT CAAGTTGTTG 
     2161 ATGTGAGAGA AGGACCTGCA TTTCATCCAA GTACTATGGC TTTTAGTGTG CGGGAAGGAA 
     2221 TAAAAGGAAG TTCCTTATTG AATTATGTGC TTGGCACATA TACAGCCATA GATTTGGACA 
     2281 CAGGAAACCC TGCAACAGAT GTCAGATATA TCATAGGGCA TGATGCAGGC AGCTGGTTAA 
     2341 AAATTGATTC AAGAACTGGT GAGATACAAT TTTCTAGAGA ATTTGATAAG AAGTCAAAAT 
     2401 ATATTATCAA TGGGATATAC ACAGCAGAGA TCCTGGCTAT AGATGATGGC TCTGGAAAAA 
     2461 CAGCTACAGG AACCATATGT ATTGAGGTTC CTGATATCAA TGATTATTGT CCAAACATTT 
     2521 TTCCTGAAAG AAGAACCATC TGCATTGACT CTCCATCAGT CCTTATCTCT GTTAATGAAC 
     2581 ATTCTTATGG GTCTCCGTTT ACTTTCTGTG TTGTTGATGA GCCACCAGGA ATAGCTGACA 
     2641 TGTGGGATGT CAGATCAACA AATGCTACCT CGGCAATCCT TACGGCTAAG CAGGTTTTAT 
     2701 CTCCAGGATT TTATGAAATC CCAATCCTGG TGAAGGACAG CTATAACAGA GCATGTGAAT 
     2761 TGGCACAAAT GGTGCAGTTA TATGCCTGTG ATTGCGATGA CAACCACATG TGCCTGGACT 
     2821 CTGGTGCCGC GGGCATCTAC ACAGAGGACA TAACTGGTGA CACGTATGGG CCTGTCACTG 
     2881 AAGACCAAGC TGGAGTTTCA AATGTTGGTC TTGGACCAGC AGGGATTGGC ATGATGGTTC 
     2941 TGGGCATCCT GCTACTGCTT TTGGCTCCAC TCTTGCTGCT CCTGTGTTGC TGCAAACAGA 
     3001 GACAGCCAGA AGGCCTGGGA ACAAGATTTG CTCCTGTGCC TGAGGGCGGA GAAGGAGTGA 
     3061 TGCAGTCTTG GAGAATTGAA GGGGCCCATC CCGAGGACAG GGATGTGTCA AATATATGTG 
     3121 CACCCATGAC AGCCTCAAAT ACCCAGGATC GGATGGATTC CTCTGAAATC TACACCAACA 
     3181 CCTATGCAGC CGGGGGCACG GTGGAAGGAG GTGTATCGGG AGTGGAGCTC AACACAGGTA 
     3241 TGGGGACAGC CGTTGGCCTC ATGGCCGCAG GGGCCGCAGG AGCCTCAGGG GCCGCAAGGA 
     3301 AGAGGAGCTC TACCATGGGA ACCCTGCGGG ACTACGCTGA CGCAGACATC AACATGGCTT 
     3361 TCTTGGACAG CTACTTCTCG GAGAAAGCGT ATGCTTATGC AGATGAAGAT GAAGGTCGAC 
     3421 CAGCCAATGA CTGCTTGCTC ATTTATGACC ACGAGGGAGT CGGGTCTCCC GTAGGCTCTA 
     3481 TTGGTTGTTG CAGTTGGATT GTGGATGACT TAGATGAAAG CTGCATGGAA ACTTTAGATC 
     3541 CAAAATTTAG GACTCTTGCT GAGATCTGCT TAAACACAGA AATTGAACCA TTTCCTTCAC 
     3601 ACCAGGCTTG TATACCAATC AGTACTGACC TCCCTTTGCT CGGACCTAAT TACTTTGTTA 
     3661 ATGAATCTTC AGGATTGACT CCCTCAGAAG TTGAATTCCA AGAAGAAATG GCAGCATCTG 
     3721 AACCCGTGGT CCATGGGGAT ATTATTGTGA CTGAGACTTA CGGTAATGCT GATCCATGTG 
     3781 TGCAACCCAC TACAATTATT TTTGATCCTC AGCTTGCACC CAATGTTGTA GTAACCGAAG 
     3841 CAGTAATGGC ACCTGTCTAT GATATTCAAG GGAATATTTG TGTACCTGCT GAGTTAGCAG 
     3901 ATTACAACAA TGTAATCTAT GCTGAGAGAG TACTGGCTAG TCCTGGTGTG CCTGACATGA 
     3961 GCAATAGTAG CACGACTGAG GGTTGTATGG GACCTGTGAT GAGCGGCAAT ATTTTAGTAG 
     4021 GGCCAGAAAT TCAAGTGATG CAAATGATGA GTCCAGACCT TCCCATAGGC CAAACCGTTG 
     4081 GCTCCACATC CCCAATGACA TCTCGACACA GAGTAACACG ATACAGTAAC ATACATTACA 
     4141 CCCAACAGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4201 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4261 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4321 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4381 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4441 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4501 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4561 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4621 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4681 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4741 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4801 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4861 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4921 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4981 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5041 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5101 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5161 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5221 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5281 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5341 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5401 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5461 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5521 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5581 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5641 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5701 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5761 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5821 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5881 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5941 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6001 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6061 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6121 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6181 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6241 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6301 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6361 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6421 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6481 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6541 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6601 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6661 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6721 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6781 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6841 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6901 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6961 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7021 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7081 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7141 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7201 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7261 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7321 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7381 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7441 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7501 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7561 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7621 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7681 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7741 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7801 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7861 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7921 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7981 CAAAAATTTA ACGCGAATTT T
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