LOCUS       RC214036                7548 bp    DNA     circular  SYN 
DEFINITION  HCN2 (Myc-DDK-tagged)-Human hyperpolarization activated cyclic nucleotide-gated potassium channel 2 (HCN2)
ACCESSION   NM_001194
VERSION     NM_001194.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3705..3796 
                     /label="Myc-DDK"  
     misc_feature    3826..4411 
                     /label="PolyA signal"  
     misc_feature    3883..3902 
                     /label="Restriction Enzyme"  
     misc_feature    4687..5359 
                     /label="ColE1"  
     misc_feature    5789..6583 
                     /label="Kan/Neo"  
     misc_feature    6618..6920 
                     /label="SV40 ori"  
     misc_feature    7006..7483 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3687..3693 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3687 
                     /label="HCN2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACGCGCGC GGGGGCGGCG GGCGGCCCGG GGAGAGCCCG GGCGCGACCC 
     1081 CCGCGCCGGG GCCGCCGCCG CCGCCGCCGC CCGCGCCCCC CCAACAGCAG CCGCCGCCGC 
     1141 CGCCGCCGCC CGCGCCCCCC CCGGGCCCCG GGCCCGCGCC CCCCCAGCAC CCGCCCCGGG 
     1201 CCGAGGCGTT GCCCCCGGAG GCGGCGGATG AGGGCGGCCC GCGGGGCCGG CTCCGCAGCC 
     1261 GCGACAGCTC GTGCGGCCGC CCCGGCACCC CGGGCGCGGC GAGCACGGCC AAGGGCAGCC 
     1321 CGAACGGCGA GTGCGGGCGC GGCGAGCCGC AGTGCAGCCC CGCGGGGCCC GAGGGCCCGG 
     1381 CGCGGGGGCC CAAGGTGTCG TTCTCGTGCC GCGGGGCGGC CTCGGGGCCC GCGCCGGGGC 
     1441 CGGGGCCGGC GGAGGAGGCG GGCAGCGAGG AGGCGGGCCC GGCGGGGGAG CCGCGCGGCA 
     1501 GCCAGGCCAG CTTCATGCAG CGCCAGTTCG GCGCGCTCCT GCAGCCGGGC GTCAACAAGT 
     1561 TCTCGCTGCG GATGTTCGGC AGCCAGAAGG CCGTGGAGCG CGAGCAGGAG CGCGTCAAGT 
     1621 CGGCGGGGGC CTGGATCATC CACCCGTACA GCGACTTCAG GTTCTACTGG GACTTCACCA 
     1681 TGCTGCTGTT CATGGTGGGA AACCTCATCA TCATCCCAGT GGGCATCACC TTCTTCAAGG 
     1741 ATGAGACCAC TGCCCCGTGG ATCGTGTTCA ACGTGGTCTC GGACACCTTC TTCCTCATGG 
     1801 ACCTGGTGTT GAACTTCCGC ACCGGCATTG TGATCGAGGA CAACACGGAG ATCATCCTGG 
     1861 ACCCCGAGAA GATCAAGAAG AAGTATCTGC GCACGTGGTT CGTGGTGGAC TTCGTGTCCT 
     1921 CCATCCCCGT GGACTACATC TTCCTTATTG TGGAGAAGGG CATTGACTCC GAGGTCTACA 
     1981 AGACGGCACG TGCCCTGCGC ATCGTGCGCT TCACCAAGAT CCTCAGCCTC CTGCGGCTGC 
     2041 TGCGCCTCTC ACGCCTGATC CGCTACATCC ATCAGTGGGA GGAGATCTTC CACATGACCT 
     2101 ATGACCTGGC CAGCGCGGTG ATGAGGATCT GCAATCTCAT CAGCATGATG CTGCTGCTCT 
     2161 GCCACTGGGA CGGCTGCCTG CAGTTCCTGG TGCCTATGCT GCAGGACTTC CCGCGCAACT 
     2221 GCTGGGTGTC CATCAATGGC ATGGTGAACC ACTCGTGGAG TGAACTGTAC TCCTTCGCAC 
     2281 TCTTCAAGGC CATGAGCCAC ATGCTGTGCA TCGGGTACGG CCGGCAGGCG CCCGAGAGCA 
     2341 TGACGGACAT CTGGCTGACC ATGCTCAGCA TGATTGTGGG TGCCACCTGC TACGCCATGT 
     2401 TCATCGGCCA CGCCACTGCC CTCATCCAGT CGCTGGACTC CTCGCGGCGC CAGTACCAGG 
     2461 AGAAGTACAA GCAGGTGGAG CAGTACATGT CCTTCCACAA GCTGCCAGCT GACTTCCGCC 
     2521 AGAAGATCCA CGACTACTAT GAGCACCGTT ACCAGGGCAA GATGTTTGAC GAGGACAGCA 
     2581 TCCTGGGCGA GCTCAACGGG CCCCTGCGGG AGGAGATCGT CAACTTCAAC TGCCGGAAGC 
     2641 TGGTGGCCTC CATGCCGCTG TTCGCCAACG CCGACCCCAA CTTCGTCACG GCCATGCTGA 
     2701 CCAAGCTCAA GTTCGAGGTC TTCCAGCCGG GTGACTACAT CATCCGCGAA GGCACCATCG 
     2761 GGAAGAAGAT GTACTTCATC CAGCACGGCG TGGTCAGCGT GCTCACTAAG GGCAACAAGG 
     2821 AGATGAAGCT GTCCGATGGC TCCTACTTCG GGGAGATCTG CCTGCTCACC CGGGGCCGCC 
     2881 GCACGGCGAG CGTGCGGGCC GACACCTACT GCCGCCTCTA TTCGCTGAGC GTGGACAACT 
     2941 TCAACGAGGT GCTGGAGGAG TACCCCATGA TGCGGCGCGC CTTCGAGACG GTGGCCATCG 
     3001 ACCGCCTGGA CCGCATCGGC AAGAAGAATT CCATCCTCCT GCACAAGGTG CAGCATGACC 
     3061 TCAACTCGGG CGTATTCAAC AACCAGGAGA ACGCCATCAT CCAGGAGATC GTCAAGTACG 
     3121 ACCGCGAGAT GGTGCAGCAG GCCGAGCTGG GTCAGCGCGT GGGCCTCTTC CCGCCGCCGC 
     3181 CGCCGCCGCC GCAGGTCACC TCGGCCATCG CCACGCTGCA GCAGGCGGCG GCCATGAGCT 
     3241 TCTGCCCGCA GGTGGCGCGG CCGCTCGTGG GGCCGCTGGC GCTCGGCTCG CCGCGCCTCG 
     3301 TGCGCCGCCC GCCCCCGGGG CCCGCACCTG CCGCCGCCTC ACCCGGGCCC CCGCCCCCCG 
     3361 CCAGCCCCCC GGGCGCGCCC GCCAGCCCCC GGGCACCGCG GACCTCGCCC TACGGCGGCC 
     3421 TGCCCGCCGC CCCCCTTGCT GGGCCCGCCC TGCCCGCGCG CCGCCTGAGC CGCGCGTCGC 
     3481 GCCCACTGTC CGCCTCGCAG CCCTCGCTGC CTCACGGCGC CCCCGGCCCC GCGGCCTCCA 
     3541 CACGCCCGGC CAGCAGCTCC ACACCGCGCT TGGGGCCCAC GCCCGCTGCC CGGGCCGCCG 
     3601 CGCCCAGCCC GGACCGCAGG GACTCGGCCT CACCCGGCGC CGCCGGCGGC CTGGACCCCC 
     3661 AGGACTCCGC GCGCTCGCGC CTCTCGTCCA ACTTGACGCG TACGCGGCCG CTCGAGCAGA 
     3721 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3781 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3841 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3901 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3961 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4021 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4081 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4141 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4201 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4261 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4321 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4381 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4441 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4501 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4561 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4621 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4681 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4741 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4801 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4861 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4921 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4981 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5041 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5101 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5161 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5221 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5281 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5341 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5401 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5461 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5521 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5581 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5641 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5701 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5761 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5821 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5881 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     5941 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6001 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6061 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6121 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6181 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6241 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6301 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6361 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6421 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6481 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6541 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6601 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6661 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6721 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6781 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6841 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6901 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     6961 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7021 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7081 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7141 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7201 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7261 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7321 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7381 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7441 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7501 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//