LOCUS       RC214026                8709 bp    DNA     circular  SYN 
DEFINITION  PRDM16 (Myc-DDK-tagged)-Human PR domain containing 16 (PRDM16), transcript variant 1
ACCESSION   NM_022114
VERSION     NM_022114.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4866..4957 
                     /label="Myc-DDK"  
     misc_feature    4987..5572 
                     /label="PolyA signal"  
     misc_feature    5044..5063 
                     /label="Restriction Enzyme"  
     misc_feature    5848..6520 
                     /label="ColE1"  
     misc_feature    6950..7744 
                     /label="Kan/Neo"  
     misc_feature    7779..8081 
                     /label="SV40 ori"  
     misc_feature    8167..8644 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4848..4854 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4848 
                     /label="PRDM16"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGATCCAAG GCGAGGGCGA GGAAGCTAGC CAAAAGTGAC GGTGACGTTG 
     1081 TAAATAATAT GTATGAGCCC AACCGGGACC TGCTGGCCAG CCACAGCGCG GAGGACGAGG 
     1141 CCGAGGACAG TGCCATGTCG CCCATCCCCG TGGGGCCACC GTCCCCCTTC CCCACCAGCG 
     1201 AGGACTTCAC CCCCAAGGAG GGCTCGCCGT ACGAGGCCCC TGTCTACATT CCTGAAGACA 
     1261 TTCCGATCCC AGCAGACTTC GAGCTCCGAG AGTCCTCCAT CCCAGGGGCT GGCCTGGGGG 
     1321 TCTGGGCCAA GAGGAAGATG GAAGCCGGGG AGAGGCTGGG CCCCTGCGTG GTGGTGCCCC 
     1381 GGGCGGCGGC AAAGGAGACA GACTTCGGAT GGGAGCAAAT ACTGACGGAC GTGGAAGTGT 
     1441 CGCCCCAGGA AGGCTGCATC ACAAAGATCT CCGAAGACCT GGGCAGTGAG AAGTTCTGCG 
     1501 TGGATGCAAA TCAGGCGGGG GCTGGCAGCT GGCTCAAGTA CATCCGTGTG GCGTGCTCCT 
     1561 GCGATGACCA GAACCTCACC ATGTGTCAGA TCAGTGAGCA GATTTACTAT AAAGTCATTA 
     1621 AGGACATTGA GCCAGGTGAG GAGCTGCTGG TGCACGTGAA GGAAGGCGTC TACCCCCTGG 
     1681 GCACAGTGCC GCCCGGCCTG GACGAGGAGC CCACGTTCCG CTGTGACGAG TGTGACGAAC 
     1741 TCTTCCAGTC CAAGCTGGAC CTGCGGCGCC ATAAGAAGTA CACGTGTGGC TCAGTGGGGG 
     1801 CTGCGCTCTA CGAGGGCCTG GCTGAGGAGC TCAAGCCCGA GGGCCTTGGC GGTGGCAGCG 
     1861 GCCAAGCCCA CGAGTGCAAG GACTGCGAGC GGATGTTCCC CAACAAGTAC AGCCTGGAGC 
     1921 AGCACATGGT CATCCACACG GAGGAGCGCG AGTACAAATG CGACCAGTGT CCCAAGGCCT 
     1981 TCAACTGGAA GTCCAACCTC ATCCGCCACC AGATGTCCCA CGACAGCGGC AAACGCTTCG 
     2041 AATGTGAAAA CTGCGTGAAG GTGTTCACGG ACCCCAGCAA CCTTCAGCGG CACATCCGCT 
     2101 CGCAGCACGT GGGCGCTCGG GCCCACGCCT GCCCCGACTG CGGGAAGACC TTCGCCACGT 
     2161 CCTCCGGCCT CAAGCAGCAC AAGCATATCC ACAGCACGGT GAAGCCTTTC ATATGTGAGG 
     2221 TCTGCCACAA GTCCTACACG CAGTTCTCCA ACCTGTGCCG GCACAAGCGG ATGCACGCCG 
     2281 ACTGCCGCAC GCAGATCAAG TGCAAGGACT GTGGCCAGAT GTTCAGCACT ACCTCCTCCC 
     2341 TCAACAAGCA CCGGCGCTTC TGCGAGGGCA AGAACCATTA CACGCCGGGC GGCATCTTTG 
     2401 CCCCGGGCCT GCCCTTGACC CCCAGCCCCA TGATGGACAA GGCAAAACCC TCCCCCAGCC 
     2461 TCAATCACGC CAGCCTGGGC TTCAACGAGT ACTTTCCCTC CAGGCCGCAC CCGGGGAGCC 
     2521 TGCCCTTCTC CACGGCGCCT CCCACGTTCC CCGCACTCAC CCCCGGCTTC CCGGGCATCT 
     2581 TCCCTCCATC CTTGTACCCC CGGCCGCCTC TGCTACCTCC CACATCGCTG CTCAAGAGCC 
     2641 CCCTGAACCA CACCCAGGAC GCCAAGCTCC CCAGTCCCCT GGGGAACCCA GCCCTGCCCC 
     2701 TGGTCTCCGC CGTCAGCAAC AGCAGCCAGG GCACGACGGC AGCTGCGGGG CCCGAGGAGA 
     2761 AGTTCGAGAG CCGCCTGGAG GACTCCTGTG TGGAGAAGCT GAAGACCAGG AGCAGCGACA 
     2821 TGTCGGACGG CAGTGACTTT GAGGACGTCA ACACCACCAC GGGGACCGAC CTGGACACGA 
     2881 CCACGGGGAC GGGCTCGGAC CTGGACAGCG ACGTGGACAG CGACCCTGAC AAGGACAAGG 
     2941 GCAAGGGCAA GTCCGCCGAG GGCCAGCCCA AGTTTGGGGG CGGCTTGGCG CCCCCGGGGG 
     3001 CCCCGAACAG CGTGGCCGAG GTGCCTGTCT TCTATTCCCA GCACTCATTC TTCCCGCCAC 
     3061 CCGACGAGCA GCTGCTGACT GCAACGGGCG CCGCCGGGGA CTCCATCAAG GCCATCGCAT 
     3121 CCATTGCCGA GAAGTACTTT GGCCCCGGCT TCATGGGGAT GCAGGAGAAG AAGCTGGGCT 
     3181 CGCTCCCCTA CCACTCGGCG TTCCCCTTCC AGTTCCTGCC CAACTTCCCC CACTCCCTTT 
     3241 ACCCCTTCAC GGACCGAGCC CTCGCCCACA ACTTGCTGGT CAAGGCCGAG CCAAAGTCAC 
     3301 CCCGGGACGC CCTCAAGGTG GGCGGCCCCA GTGCCGAGTG CCCCTTTGAT CTCACCACCA 
     3361 AGCCCAAAGA CGTGAAGCCC ATCCTGCCCA TGCCCAAGGG CCCCTCGGCC CCCGCATCCG 
     3421 GCGAGGAGCA GCCGCTGGAC CTGAGCATCG GCAGCCGGGC CCGTGCCAGC CAAAACGGCG 
     3481 GCGGGCGGGA GCCCCGCAAG AACCACGTCT ATGGGGAACG CAAGCTGGGC GCCGGCGAGG 
     3541 GGCTGCCCCA GGTGTGCCCG GCGCGGATGC CCCAGCAGCC CCCGCTCCAC TACGCCAAGC 
     3601 CCTCGCCCTT CTTCATGGAC CCCATCTACA GCAGGGTAGA AAAGCGGAAG GTCACAGACC 
     3661 CCGTGGGAGC CCTGAAGGAG AAGTACCTGC GGCCGTCCCC GCTGCTCTTC CACCCCCAGA 
     3721 TGTCAGCCAT AGAGACCATG ACAGAGAAGC TGGAGAGCTT TGCAGCCATG AAGGCGGACT 
     3781 CGGGCAGCTC CCTGCAGCCC CTCCCCCACC ACCCCTTCAA CTTCCGGTCC CCACCCCCAA 
     3841 CGCTCTCCGA CCCCATCCTC AGGAAGGGCA AGGAGCGATA CACGTGCAGG TACTGTGGGA 
     3901 AGATCTTCCC CAGATCAGCC AATCTCACCA GACACCTGAG GACGCACACT GGGGAGCAGC 
     3961 CGTACAGGTG TAAGTACTGC GACCGCTCCT TCAGCATCTC TTCGAACCTC CAGCGGCACG 
     4021 TCCGGAACAT CCACAACAAG GAGAAGCCTT TCAAGTGCCA CCTGTGCAAC CGCTGCTTCG 
     4081 GGCAGCAGAC CAACCTGGAC CGGCACCTCA AGAAGCACGA GCACGAGAAC GCACCAGTGA 
     4141 GCCAGCACCC CGGGGTCCTC ACGAACCACC TGGGGACCAG CGCGTCCTCT CCCACCTCAG 
     4201 AGTCGGACAA CCACGCACTT TTAGACGAGA AAGAAGACTC TTATTTCTCG GAAATCAGAA 
     4261 ACTTTATTGC CAATAGTGAG ATGAACCAAG CATCAACGCG AACAGAGAAA CGGGCGGACA 
     4321 TGCAGATCGT GGACGGCAGT GCCCAGTGTC CAGGCCTAGC CAGTGAGAAG CAGGAGGACG 
     4381 TGGAGGAGGA GGACGACGAT GACCTGGAGG AGGACGATGA GGACAGCCTG GCCGGGAAGT 
     4441 CGCAGGATGA CACCGTGTCC CCCGCACCCG AGCCCCAGGC CGCCTACGAG GATGAGGAGG 
     4501 ATGAGGAGCC AGCCGCCTCC CTGGCCGTGG GCTTTGACCA CACCCGAAGG TGTGCTGAGG 
     4561 ACCACGAAGG CGGTCTGTTA GCTTTGGAGC CGATGCCGAC TTTTGGGAAG GGGCTGGACC 
     4621 TCCGCAGAGC AGCTGAGGAA GCATTTGAAG TTAAAGATGT GCTTAATTCC ACCTTAGATT 
     4681 CTGAGGCTTT AAAACATACA CTGTGCAGGC AGGCTAAGAA CCAGGCATAT GCAATGATGC 
     4741 TGTCCCTTTC CGAAGACACT CCTCTCCACA CCCCCTCCCA GGGTTCTCTG GACGCTTGGT 
     4801 TGAAGGTCAC TGGAGCCACG TCGGAGTCTG GAGCATTTCA CCCCATCAAC CACCTCACGC 
     4861 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4921 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4981 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5041 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5101 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5161 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5221 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5281 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5341 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5401 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5461 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5521 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5581 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5641 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5701 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5761 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5821 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5881 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5941 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6001 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6061 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6121 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6181 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6241 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6301 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6361 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6421 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6481 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6541 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6601 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6661 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6721 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6781 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6841 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6901 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6961 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7021 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7081 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7141 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7201 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7261 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7321 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7381 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7441 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7501 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7561 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7621 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7681 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7741 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7801 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7861 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7921 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7981 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8041 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8101 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8161 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8221 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8281 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8341 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8401 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8461 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8521 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8581 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8641 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8701 GCGAATTTT
//