LOCUS       RC214008                8181 bp    DNA     circular  SYN 
DEFINITION  FMNL1 (Myc-DDK-tagged)-Human formin-like 1 (FMNL1)
ACCESSION   NM_005892
VERSION     NM_005892.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4338..4429 
                     /label="Myc-DDK"  
     misc_feature    4459..5044 
                     /label="PolyA signal"  
     misc_feature    4516..4535 
                     /label="Restriction Enzyme"  
     misc_feature    5320..5992 
                     /label="ColE1"  
     misc_feature    6422..7216 
                     /label="Kan/Neo"  
     misc_feature    7251..7553 
                     /label="SV40 ori"  
     misc_feature    7639..8116 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4320..4326 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4320 
                     /label="FMNL1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCAACGCG GCCGGCAGCG CCGAGCAGCC CGCGGGCCCC GCCGCGCCGC 
     1081 CCCCCAAGCA GCCCGCGCCT CCCAAGCAGC CGATGCCCGC GGCCGGAGAG CTGGAGGAGA 
     1141 GGTTCAACCG CGCCCTGAAC TGCATGAACT TGCCCCCAGA CAAGGTCCAG CTGCTGAGCC 
     1201 AGTATGACAA CGAGAAGAAG TGGGAGCTCA TCTGTGATCA GGAGCGGTTT CAAGTCAAGA 
     1261 ATCCCCCCGC AGCCTACATC CAGAAGCTGA AGAGCTATGT GGATACTGGT GGGGTCAGCC 
     1321 GAAAGGTAGC AGCTGATTGG ATGTCCAACC TGGGGTTTAA GAGGCGAGTT CAGGAGTCCA 
     1381 CGCAGGTGCT ACGGGAGCTG GAGACCTCCC TGAGGACCAA CCACATTGGG TGGGTGCAGG 
     1441 AGTTCCTCAA TGAAGAGAAC CGTGGCCTGG ATGTGCTGCT CGAGTACCTG GCCTTTGCCC 
     1501 AGTGCTCTGT CACGTATGAC ATGGAGAGCA CAGACAACGG GGCTTCCAAC TCAGAGAAAA 
     1561 ACAAGCCCCT GGAGCAGTCT GTGGAAGACC TCAGCAAGGG TCCACCCTCC TCCGTGCCCA 
     1621 AAAGCCGCCA CCTGACCATC AAGCTGACCC CAGCCCACAG CAGGAAGGCC CTGCGGAATT 
     1681 CCCGCATCGT CAGCCAGAAG GACGACGTCC ACGTCTGTAT TATGTGCCTA CGCGCCATCA 
     1741 TGAACTACCA GTCTGGCTTC AGCCTTGTCA TGAACCACCC AGCCTGTGTC AATGAGATTG 
     1801 CTCTGAGCCT CAACAACAAG AACCCCAGAA CCAAGGCTCT GGTGCTGGAG CTGCTGGCGG 
     1861 CCGTGTGCTT GGTGCGGGGA GGACATGACA TCATCCTTGC AGCCTTTGAC AACTTCAAGG 
     1921 AGGTGTGTGG GGAGCAGCAC CGCTTTGAAA AGCTGATGGA ATATTTCCGG AATGAGGACA 
     1981 GCAACATCGA CTTCATGGTG GCCTGCATGC AGTTCATCAA CATTGTGGTA CATTCAGTGG 
     2041 AGAACATGAA CTTCCGTGTC TTCCTGCAAT ATGAGTTCAC CCACTTGGGC CTGGACCTGT 
     2101 ACTTGGAGAG GCTTCGGCTC ACCGAGAGTG ACAAGCTGCA GGTGCAGATC CAGGCGTACC 
     2161 TGGACAATAT TTTTGATGTG GGGGCGCTGC TGGAGGACAC AGAGACCAAG AACGCTGTGC 
     2221 TGGAGCACAT GGAGGAACTG CAGGAGCAAG TGGCGCTGCT GACAGAGCGG CTTCGGGACG 
     2281 CGGAGAACGA ATCCATGGCC AAGATTGCAG AACTGGAAAA ACAGCTAAGC CAGGCGCGCA 
     2341 AGGAGTTGGA GACCCTGCGG GAGCGCTTCA GCGAATCGAC CGCCATGGGC GCCTCCAGGC 
     2401 GTCCCCCAGA GCCTGAGAAA GCGCCTCCCG CTGCCCCGAC GCGGCCCTCG GCCCTGGAGC 
     2461 TGAAGGTGGA GGAGCTGGAG GAGAAGGGGT TAATCCGTAT TCTGCGGGGG CCGGGGGATG 
     2521 CTGTCTCCAT CGAGATCCTC CCCGTCGCTG TGGCAACTCC GAGCGGCGGT GATGCTCCGA 
     2581 CTCCGGGGGT GCCGACCGGC TCCCCCAGCC CAGATCTCGC ACCTGCAGCA GAGCCGGCTC 
     2641 CCGGAGCAGC GCCACCGCCG CCGCCCCCAC TGCCCGGCCT CCCCTCCCCG CAGGAAGCCC 
     2701 CGCCCTCTGC GCCCCCACAG GCCCCGCCTC TCCCTGGCAG CCCGGAGCCC CCGCCTGCGC 
     2761 CGCCGCTGCC CGGAGACCTG CCGCCCCCAC CCCCGCCACC GCCACCACCT CCGGGCACTG 
     2821 ACGGGCCGGT GCCTCCGCCG CCGCCGCCGC CGCCGCCGCC TCCCGGAGGT CCTCCTGATG 
     2881 CCCTAGGAAG ACGCGACTCA GAATTGGGCC CAGGAGTGAA GGCCAAGAAG CCCATCCAGA 
     2941 CTAAGTTCCG AATGCCACTC TTGAACTGGG TGGCACTGAA ACCCAGCCAG ATCACCGGCA 
     3001 CTGTCTTCAC AGAGCTCAAT GATGAGAAGG TGCTGCAGGA GCTAGACATG AGTGATTTTG 
     3061 AGGAACAGTT CAAGACCAAG TCCCAAGGCC CCAGCCTGGA CCTCAGCGCT CTCAAGAGTA 
     3121 AGGCAGCCCA GAAGGCCCCC AGCAAGGCGA CACTCATTGA GGCCAACCGG GCCAAGAACT 
     3181 TGGCCATCAC CCTGCGGAAG GGCAACCTGG GGGCCGAGCG CATCTGCCAA GCCATTGAGG 
     3241 CGTACGACCT GCAGGCTCTG GGCCTGGACT TCCTGGAGCT GCTGATGCGC TTCCTGCCCA 
     3301 CAGAGTATGA GCGCAGCCTC ATCACCCGCT TTGAGCGGGA GCAGCGGCCA ATGGAGGAGC 
     3361 TGTCAGAGGA GGACCGCTTC ATGCTATGCT TCAGCCGCAT CCCGCGCCTG CCGGAGCGCA 
     3421 TGACCACACT CACCTTCCTG GGCAACTTCC CGGACACAGC CCAGCTGCTC ATGCCGCAAC 
     3481 TGAATGCCAT CATTGCAGCC TCAATGTCCA TCAAGTCCTC TGACAAACTC CGCCAGATCC 
     3541 TGGAGATTGT CCTGGCCTTT GGCAACTACA TGAACAGTAG CAAGCGTGGG GCAGCCTATG 
     3601 GCTTCCGGCT CCAGAGCCTG GATGCGCTGT TGGAGATGAA GTCGACTGAT CGCAAGCAGA 
     3661 CGCTGCTGCA CTACCTGGTG AAGGTCATTG CTGAGAAGTA CCCGCAACTC ACAGGCTTCC 
     3721 ACAGCGACCT GCACTTCCTG GACAAGGCGG GCTCAGTGTC CCTGGACAGT GTCCTGGCGG 
     3781 ACGTGCGCTC CCTGCAGCGA GGCCTAGAGT TGACACAGAG AGAGTTTGTG CGGCAGGATG 
     3841 ACTGCATGGT GCTCAAGGAG TTCCTGAGGG CCAACTCGCC CACCATGGAC AAGCTGCTGG 
     3901 CAGACAGCAA GACGGCTCAG GAGGCCTTTG AGTCTGTGGT GGAGTACTTC GGAGAGAACC 
     3961 CCAAGACCAC ATCCCCAGGC CTGTTCTTCT CCCTCTTTAG CCGCTTCATT AAGGCCTACA 
     4021 AGAAAGCTGA GCAGGAGGTG GAACAGTGGA AAAAAGAAGC CGCTGCCCAG GAGGCAGGCG 
     4081 CTGATACCCC GGGCAAAGGG GAGCCCCCAG CACCCAAGTC ACCGCCAAAG GCCCGGCGGC 
     4141 CACAGATGGA CCTCATCTCT GAGCTGAAAC GGAGGCAGCA GAAGGAGCCA CTCATTTATG 
     4201 AGAGCGACCG TGATGGGGCC ATTGAAGACA TCATCACAGT GATCAAGACG GTGCCCTTCA 
     4261 CGGCCCGCAC CGGCAAGCGG ACATCCCGGC TCCTCTGTGA GGCCAGCCTG GGAGAAGAGA 
     4321 TGCCCCTCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4381 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4441 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4501 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4561 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4621 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4681 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4741 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4801 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4861 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4921 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4981 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5041 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5101 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5161 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5221 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5281 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5341 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5401 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5461 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5521 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5581 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5641 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5701 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5761 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5821 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5881 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5941 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6001 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6061 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6121 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6181 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6241 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6301 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6361 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6421 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6481 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6541 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6601 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6661 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6721 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6781 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6841 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6901 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6961 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7021 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7081 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7141 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7201 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7261 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7321 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7381 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7441 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7501 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7561 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7621 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7681 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7741 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7801 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7861 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7921 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7981 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8041 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8101 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8161 CAAAAATTTA ACGCGAATTT T
//