LOCUS       RC213986                8187 bp    DNA     circular  SYN 
DEFINITION  USP7 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 7 (herpes virus-associated) (USP7)
ACCESSION   NM_003470
VERSION     NM_003470.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4344..4435 
                     /label="Myc-DDK"  
     misc_feature    4465..5050 
                     /label="PolyA signal"  
     misc_feature    4522..4541 
                     /label="Restriction Enzyme"  
     misc_feature    5326..5998 
                     /label="ColE1"  
     misc_feature    6428..7222 
                     /label="Kan/Neo"  
     misc_feature    7257..7559 
                     /label="SV40 ori"  
     misc_feature    7645..8122 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4326..4332 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4326 
                     /label="USP7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACCACCAG CAGCAGCAGC AGCAGCAGAA AGCGGGCGAG CAGCAGTTGA 
     1081 GCGAGCCCGA GGACATGGAG ATGGAAGCGG GAGATACAGA TGACCCACCA AGAATTACTC 
     1141 AGAACCCTGT GATCAATGGG AATGTGGCCC TGAGTGATGG ACACAACACC GCGGAGGAGG 
     1201 ACATGGAGGA TGACACCAGT TGGCGCTCCG AGGCAACCTT TCAGTTCACT GTGGAGCGCT 
     1261 TCAGCAGACT GAGTGAGTCG GTCCTTAGCC CTCCGTGTTT TGTGCGAAAT CTGCCATGGA 
     1321 AGATTATGGT GATGCCACGC TTTTATCCAG ACAGACCACA CCAAAAAAGC GTAGGATTCT 
     1381 TTCTCCAGTG CAATGCTGAA TCTGATTCCA CGTCATGGTC TTGCCATGCA CAAGCAGTGC 
     1441 TGAAGATAAT AAATTACAGA GATGATGAAA AGTCGTTCAG TCGTCGTATT AGTCATTTGT 
     1501 TCTTCCATAA AGAAAATGAT TGGGGATTTT CCAATTTTAT GGCCTGGAGT GAAGTGACCG 
     1561 ATCCTGAGAA AGGATTTATA GATGATGACA AAGTTACCTT TGAAGTCTTT GTACAGGCGG 
     1621 ATGCTCCCCA TGGAGTTGCG TGGGATTCAA AGAAGCACAC AGGCTACGTC GGCTTAAAGA 
     1681 ATCAGGGAGC GACTTGTTAC ATGAACAGCC TGCTACAGAC GTTATTTTTC ACGAATCAGC 
     1741 TACGAAAGGC TGTGTACATG ATGCCAACCG AGGGGGATGA TTCGTCTAAA AGCGTCCCTT 
     1801 TAGCATTACA AAGAGTGTTC TATGAATTAC AGCATAGTGA TAAACCTGTA GGAACAAAAA 
     1861 AGTTAACAAA GTCATTTGGG TGGGAAACTT TAGATAGCTT CATGCAACAT GATGTTCAGG 
     1921 AGCTTTGTCG AGTGTTGCTC GATAATGTGG AAAATAAGAT GAAAGGCACC TGTGTAGAGG 
     1981 GCACCATACC CAAATTATTC CGCGGCAAAA TGGTGTCCTA TATCCAGTGT AAAGAAGTAG 
     2041 ACTATCGGTC TGATAGAAGA GAAGATTATT ATGATATCCA GCTAAGTATC AAAGGAAAGA 
     2101 AAAATATATT TGAATCATTT GTGGATTATG TGGCAGTAGA ACAGCTCGAT GGGGACAATA 
     2161 AATACGACGC TGGGGAACAT GGCTTACAGG AAGCAGAGAA AGGTGTGAAA TTCCTAACAT 
     2221 TGCCACCAGT GTTACATCTA CAACTGATGA GATTTATGTA TGACCCTCAG ACGGACCAAA 
     2281 ATATCAAGAT CAATGATAGG TTTGAATTCC CAGAGCAGTT ACCACTTGAT GAATTTTTGC 
     2341 AAAAAACAGA TCCTAAGGAC CCTGCAAATT ATATTCTTCA TGCAGTCCTG GTTCATAGTG 
     2401 GAGATAATCA TGGTGGACAT TATGTGGTTT ATCTAAACCC CAAAGGGGAT GGCAAATGGT 
     2461 GTAAATTTGA TGACGACGTG GTGTCAAGGT GTACTAAAGA GGAAGCAATT GAGCACAATT 
     2521 ATGGGGGTCA CGATGACGAC CTGTCTGTTC GACACTGCAC TAATGCTTAC ATGTTAGTCT 
     2581 ACATCAGGGA ATCAAAACTG AGTGAAGTTT TACAGGCGGT CACCGACCAT GATATTCCTC 
     2641 AGCAGTTGGT GGAGCGATTA CAAGAAGAGA AAAGGATCGA GGCTCAGAAG CGGAAGGAGC 
     2701 GGCAGGAAGC CCATCTCTAT ATGCAAGTGC AGATAGTCGC AGAGGACCAG TTTTGTGGCC 
     2761 ACCAAGGGAA TGACATGTAC GATGAAGAAA AAGTGAAATA CACTGTGTTC AAAGTATTGA 
     2821 AGAACTCCTC GCTTGCTGAG TTTGTTCAGA GCCTCTCTCA GACCATGGGA TTTCCACAAG 
     2881 ATCAAATTCG ATTGTGGCCC ATGCAAGCAA GGAGTAATGG AACAAAACGA CCAGCAATGT 
     2941 TAGATAATGA AGCCGACGGC AATAAAACAA TGATTGAGCT CAGTGATAAT GAAAACCCTT 
     3001 GGACAATATT CCTGGAAACA GTTGATCCCG AGCTGGCTGC TAGTGGAGCG ACCTTACCCA 
     3061 AGTTTGATAA AGATCATGAT GTAATGTTAT TTTTGAAGAT GTATGATCCC AAAACGCGGA 
     3121 GCTTGAATTA CTGTGGGCAT ATCTACACAC CAATATCCTG TAAAATACGT GACTTGCTCC 
     3181 CAGTTATGTG TGACAGAGCA GGATTTATTC AAGATACTAG CCTTATCCTC TATGAGGAAG 
     3241 TTAAACCGAA TTTAACAGAG AGAATTCAGG ACTATGACGT GTCTCTTGAT AAAGCCCTTG 
     3301 ATGAACTAAT GGATGGTGAC ATCATAGTAT TTCAGAAGGA TGACCCTGAA AATGATAACA 
     3361 GTGAATTACC CACCGCAAAG GAGTATTTCC GAGATCTCTA CCACCGCGTT GATGTCATTT 
     3421 TCTGTGATAA AACAATCCCT AATGATCCTG GATTTGTGGT TACGTTATCA AATAGAATGA 
     3481 ATTATTTTCA GGTTGCAAAG ACAGTTGCAC AGAGGCTCAA CACAGATCCA ATGTTGCTGC 
     3541 AGTTTTTCAA GTCTCAAGGT TATAGGGATG GCCCAGGTAA TCCTCTTAGA CATAATTATG 
     3601 AAGGTACTTT AAGAGATCTT CTACAGTTCT TCAAGCCTAG ACAACCTAAG AAACTTTACT 
     3661 ATCAGCAGCT TAAGATGAAA ATCACAGACT TTGAGAACAG GCGAAGTTTT AAATGTATAT 
     3721 GGTTAAACAG CCAATTTAGG GAAGAGGAAA TAACACTATA TCCAGACAAG CATGGGTGTG 
     3781 TCCGGGACCT GTTAGAAGAA TGTAAAAAGG CCGTGGAGCT TGGGGAGAAA GCATCAGGGA 
     3841 AACTTAGGCT GCTAGAAATT GTAAGCTACA AAATCATTGG TGTTCATCAA GAAGATGAAC 
     3901 TATTAGAATG TTTATCTCCT GCAACGAGCC GGACGTTTCG AATAGAGGAA ATCCCTTTGG 
     3961 ACCAGGTGGA CATAGACAAA GAGAATGAGA TGCTTGTCAC AGTGGCGCAT TTCCACAAAG 
     4021 AGGTCTTCGG AACGTTCGGA ATCCCGTTTT TGCTGAGGAT ACACCAGGGC GAGCATTTTC 
     4081 GAGAAGTGAT GAAGCGAATC CAGAGCCTGC TGGACATCCA GGAGAAGGAG TTTGAGAAGT 
     4141 TTAAATTTGC AATTGTAATG ATGGGCCGAC ACCAGTACAT AAATGAAGAC GAGTATGAAG 
     4201 TAAATTTGAA AGACTTTGAG CCACAGCCCG GTAATATGTC TCATCCTCGG CCTTGGCTAG 
     4261 GGCTCGACCA CTTCAACAAA GCCCCAAAGA GGAGTCGCTA CACTTACCTT GAAAAGGCCA 
     4321 TTAAAATCCA TAACACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4381 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4441 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4501 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4561 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4621 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4681 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4741 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4801 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4861 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4921 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4981 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5041 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5101 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5161 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5221 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5281 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5341 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5401 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5461 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5521 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5581 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5641 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5701 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5761 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5821 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5881 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5941 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6001 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6061 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6121 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6181 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6241 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6301 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6361 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6421 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6481 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6541 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6601 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6661 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6721 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6781 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6841 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6901 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6961 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7021 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7081 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7141 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7201 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7261 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7321 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7381 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7441 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7501 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7561 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7621 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7681 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7741 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7801 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7861 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7921 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7981 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8041 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8101 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8161 ATTTAACAAA AATTTAACGC GAATTTT
//