LOCUS       RC213971                8577 bp    DNA     circular  SYN 
DEFINITION  KIF4A (Myc-DDK-tagged)-Human kinesin family member 4A (KIF4A). Note: ORF is codon optimized
ACCESSION   NM_012310
VERSION     NM_012310.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4734..4825 
                     /label="Myc-DDK"  
     misc_feature    4855..5440 
                     /label="PolyA signal"  
     misc_feature    4912..4931 
                     /label="Restriction Enzyme"  
     misc_feature    5716..6388 
                     /label="ColE1"  
     misc_feature    6818..7612 
                     /label="Kan/Neo"  
     misc_feature    7647..7949 
                     /label="SV40 ori"  
     misc_feature    8035..8512 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4716..4722 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4716 
                     /label="KIF4A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGGAAGAG GTCAAGGGCA TACCCGTGCG AGTTGCGTTG CGCTGTCGCC 
     1081 CACTGGTGCC AAAGGAAATT TCTGAGGGAT GCCAGATGTG TCTCTCCTTT GTCCCGGGGG 
     1141 AGCCGCAGGT CGTGGTCGGC ACTGACAAGA GCTTTACATA CGATTTCGTT TTTGACCCTA 
     1201 GCACAGAGCA GGAGGAGGTA TTTAATACCG CGGTCGCACC CCTCATAAAG GGAGTGTTTA 
     1261 AGGGCTACAA CGCCACCGTG TTGGCTTACG GCCAGACCGG CAGTGGCAAG ACATATTCTA 
     1321 TGGGTGGGGC ATACACCGCA GAACAGGAGA ACGAACCAAC CGTGGGGGTA ATTCCTCGAG 
     1381 TAATCCAACT TCTTTTTAAG GAGATCGATA AAAAGAGTGA CTTCGAATTT ACCCTCAAAG 
     1441 TTAGTTATCT GGAGATCTAC AACGAGGAAA TCCTGGATCT GCTGTGTCCC AGCAGGGAGA 
     1501 AAGCCCAGAT CAATATTAGG GAGGACCCCA AGGAGGGGAT CAAGATAGTG GGGCTGACCG 
     1561 AAAAGACAGT GCTCGTTGCT CTGGATACCG TGAGCTGTTT GGAACAGGGG AACAATTCAC 
     1621 GCACTGTCGC GTCCACCGCC ATGAACTCTC AGTCTTCCCG CTCTCACGCA ATTTTCACCA 
     1681 TCAGTCTTGA GCAGCGGAAA AAGAGTGACA AGAATAGCAG CTTTAGGTCC AAGCTGCATC 
     1741 TGGTGGATTT GGCCGGCAGC GAGCGGCAAA AAAAGACCAA GGCTGAGGGC GACCGGCTGA 
     1801 AAGAGGGTAT TAACATCAAT CGGGGACTTC TGTGCTTGGG AAATGTAATC TCAGCCCTGG 
     1861 GTGACGATAA GAAAGGAGGA TTCGTACCAT ACCGCGACTC AAAACTCACC AGACTGCTGC 
     1921 AGGACTCACT GGGAGGAAAC TCACACACTC TGATGATCGC GTGTGTGTCT CCTGCTGATA 
     1981 GCAATCTCGA AGAAACTCTC AATACCCTGC GCTACGCCGA CCGGGCACGC AAGATCAAAA 
     2041 ACAAACCTAT AGTGAACATC GACCCTCAGA CAGCTGAGCT CAACCACCTG AAGCAGCAAG 
     2101 TGCAGCAGCT GCAGGTCTTG CTGCTGCAGG CCCACGGCGG CACGCTGCCT GGCAGCATTA 
     2161 CTGTTGAACC AAGTGAAAAC CTGCAGTCCT TGATGGAGAA GAACCAGTCT CTCGTTGAGG 
     2221 AAAACGAAAA GCTCAGCCGA GGGCTTTCCG AGGCCGCCGG TCAAACAGCA CAAATGCTCG 
     2281 AAAGGATTAT TCTCACCGAG CAGGCCAATG AAAAGATGAA CGCAAAACTT GAGGAGCTGA 
     2341 GACAGCACGC CGCCTGTAAA CTGGACCTGC AAAAGCTGGT GGAGACTCTG GAGGACCAGG 
     2401 AGCTGAAGGA AAACGTGGAG ATCATATGTA ATCTGCAGCA ACTGATCACA CAGTTGAGCG 
     2461 ACGAGACAGT TGCCTGTATG GCTGCTGCCA TCGACACGGC AGTGGAGCAG GAAGCTCAGG 
     2521 TTGAGACTAG CCCCGAAACT AGCCGCAGTT CCGATGCGTT CACAACCCAG CACGCATTGC 
     2581 GACAGGCTCA GATGAGTAAG GAATTGGTGG AGTTGAACAA AGCTCTGGCT CTGAAGGAGG 
     2641 CCTTGGCGAG GAAGATGACC CAGAATGACT CCCAGCTGCA GCCCATCCAG TATCAGTACC 
     2701 AAGACAACAT CAAAGAACTC GAGCTTGAAG TGATCAACCT TCAGAAGGAA AAAGAAGAGC 
     2761 TGGTCCTGGA GCTTCAAACT GCAAAGAAAG ATGCAAACCA GGCCAAACTG AGTGAAAGGC 
     2821 GACGCAAGAG ATTGCAGGAG CTGGAGGGTC AGATTGCAGA CCTGAAGAAA AAACTGAACG 
     2881 AACAGTCCAA GCTCCTGAAA CTGAAGGAGA GCACAGAGCG GACCGTCTCA AAGCTGAATC 
     2941 AGGAGATCCG CATGATGAAA AACCAACGGG TACAGTTGAT GCGGCAAATG AAGGAAGACG 
     3001 CAGAGAAGTT CAGGCAGTGG AAGCAGAAAA AGGACAAAGA AGTGATCCAG CTGAAGGAAA 
     3061 GGGACCGGAA GAGGCAGTAC GAGCTTCTCA AACTGGAGCG GAACTTTCAA AAACAGTCCA 
     3121 ATGTCCTGCG GCGGAAAACC GAGGAAGCAG CAGCCGCAAA CAAGCGGCTG AAAGATGCCT 
     3181 TGCAGAAACA GCGGGAAGTT GCGGACAAGC GAAAAGAGAC TCAGAGCCGG GGCATGGAGG 
     3241 GAACCGCCGC TCGCGTAAAG AACTGGCTGG GCAATGAAAT AGAGGTGATG GTGAGCACCG 
     3301 AGGAGGCCAA ACGCCACCTC AATGACCTCC TCGAAGATCG GAAAATCCTT GCACAAGACG 
     3361 TGGCCCAACT GAAGGAGAAA AAAGAATCCG GCGAAAACCC TCCCCCTAAG TTGCGGCGAC 
     3421 GAACATTCAG CCTCACAGAG GTAAGGGGCC AGGTTAGCGA ATCAGAAGAC AGCATCACGA 
     3481 AACAGATCGA ATCCTTGGAA ACCGAGATGG AATTTAGATC CGCACAGATC GCCGACCTGC 
     3541 AGCAAAAGCT CCTGGACGCC GAGAGTGAGG ACAGGCCTAA ACAAAGGTGG GAGAACATTG 
     3601 CTACAATACT CGAAGCCAAA TGTGCCCTTA AATACTTGAT CGGCGAGCTG GTTAGCAGCA 
     3661 AAATACAGGT GAGTAAGCTT GAGAGTTCTC TCAAGCAGAG CAAAACCTCC TGCGCAGATA 
     3721 TGCAGAAAAT GCTGTTCGAG GAAAGAAATC ATTTCGCCGA GATTGAAACC GAGTTGCAAG 
     3781 CTGAGCTGGT TAGGATGGAA CAGCAGCATC AGGAGAAAGT CCTGTATCTC CTGTCACAGC 
     3841 TCCAACAGAG CCAAATGGCA GAGAAGCAGC TGGAAGAGTC AGTGAGTGAG AAGGAGCAGC 
     3901 AGCTTCTGAG CACCCTGAAA TGCCAAGACG AAGAACTCGA AAAGATGCGC GAAGTGTGCG 
     3961 AGCAGAATCA GCAGCTCCTC AGGGAAAACG AAATCATTAA ACAAAAACTC ACATTGCTGC 
     4021 AGGTGGCTAG CAGACAGAAG CACTTGCCAA AAGATACGCT GCTGAGCCCA GACAGCTCTT 
     4081 TCGAGTATGT GCCTCCAAAA CCTAAACCAT CCCGCGTGAA AGAGAAGTTT CTGGAACAGA 
     4141 GCATGGATAT CGAGGATTTG AAGTATTGCT CCGAGCATTC AGTCAATGAA CATGAGGACG 
     4201 GTGATGGTGA CGACGACGAG GGAGATGATG AAGAATGGAA ACCGACCAAA CTCGTCAAAG 
     4261 TTAGCCGCAA AAATATCCAA GGCTGTTCAT GCAAAGGATG GTGTGGCAAC AAGCAATGCG 
     4321 GTTGCAGGAA ACAGAAATCT GATTGCGGTG TAGACTGTTG TTGCGATCCT ACAAAATGCC 
     4381 GGAATCGCCA ACAGGGAAAA GACAGCCTCG GTACAGTTGA GAGAACCCAG GATTCAGAGG 
     4441 GAAGCTTCAA ACTCGAGGAT CCCACAGAGG TTACCCCAGG CCTGAGTTTC TTCAATCCCG 
     4501 TGTGCGCCAC ACCTAATAGT AAGATTCTGA AGGAGATGTG CGACGTGGAA CAGGTTCTTT 
     4561 CCAAAAAAAC CCCTCCCGCC CCATCTCCCT TTGATCTGCC TGAACTTAAG CATGTTGCTA 
     4621 CTGAGTACCA AGAAAATAAG GCCCCTGGTA AGAAGAAGAA ACGGGCTCTG GCCTCTAATA 
     4681 CCTCCTTTTT CTCTGGCTGT TCCCCAATTG AGGAGGAGGC CCACACGCGT ACGCGGCCGC 
     4741 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4801 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4861 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4921 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4981 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5041 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5101 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5161 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5221 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5281 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5341 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5401 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5461 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5521 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5581 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5641 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5701 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5761 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5821 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5881 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5941 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6001 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6061 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6121 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6181 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6241 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6301 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6361 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6421 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6481 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6541 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6601 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6661 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6721 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6781 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6841 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6901 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6961 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7021 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7081 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7141 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7201 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7261 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7321 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7381 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7441 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7501 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7561 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7621 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7681 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7741 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7801 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7861 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7921 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7981 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8041 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8101 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8161 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8221 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8281 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8341 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8401 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8461 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8521 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//