LOCUS       RC213926                8610 bp    DNA     circular  SYN 
DEFINITION  DMD (Myc-DDK-tagged)-Human dystrophin (DMD), transcript variant Dp140b
ACCESSION   NM_004021
VERSION     NM_004021.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4767..4858 
                     /label="Myc-DDK"  
     misc_feature    4888..5473 
                     /label="PolyA signal"  
     misc_feature    4945..4964 
                     /label="Restriction Enzyme"  
     misc_feature    5749..6421 
                     /label="ColE1"  
     misc_feature    6851..7645 
                     /label="Kan/Neo"  
     misc_feature    7680..7982 
                     /label="SV40 ori"  
     misc_feature    8068..8545 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4749..4755 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4749 
                     /label="DMD"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCATCTTCC TTGATGTTGG AGGTACCTGC TCTGGCAGAT TTCAACCGGG 
     1081 CTTGGACAGA ACTTACCGAC TGGCTTTCTC TGCTTGATCA AGTTATAAAA TCACAGAGGG 
     1141 TGATGGTGGG TGACCTTGAG GATATCAACG AGATGATCAT CAAGCAGAAG GCAACAATGC 
     1201 AGGATTTGGA ACAGAGGCGT CCCCAGTTGG AAGAACTCAT TACCGCTGCC CAAAATTTGA 
     1261 AAAACAAGAC CAGCAATCAA GAGGCTAGAA CAATCATTAC GGATCGAATT GAAAGAATTC 
     1321 AGAATCAGTG GGATGAAGTA CAAGAACACC TTCAGAACCG GAGGCAACAG TTGAATGAAA 
     1381 TGTTAAAGGA TTCAACACAA TGGCTGGAAG CTAAGGAAGA AGCTGAGCAG GTCTTAGGAC 
     1441 AGGCCAGAGC CAAGCTTGAG TCATGGAAGG AGGGTCCCTA TACAGTAGAT GCAATCCAAA 
     1501 AGAAAATCAC AGAAACCAAG CAGTTGGCCA AAGACCTCCG CCAGTGGCAG ACAAATGTAG 
     1561 ATGTGGCAAA TGACTTGGCC CTGAAACTTC TCCGGGATTA TTCTGCAGAT GATACCAGAA 
     1621 AAGTCCACAT GATAACAGAG AATATCAATG CCTCTTGGAG AAGCATTCAT AAAAGGGTGA 
     1681 GTGAGCGAGA GGCTGCTTTG GAAGAAACTC ATAGATTACT GCAACAGTTC CCCCTGGACC 
     1741 TGGAAAAGTT TCTTGCCTGG CTTACAGAAG CTGAAACAAC TGCCAATGTC CTACAGGATG 
     1801 CTACCCGTAA GGAAAGGCTC CTAGAAGACT CCAAGGGAGT AAAAGAGCTG ATGAAACAAT 
     1861 GGCAAGACCT CCAAGGTGAA ATTGAAGCTC ACACAGATGT TTATCACAAC CTGGATGAAA 
     1921 ACAGCCAAAA AATCCTGAGA TCCCTGGAAG GTTCCGATGA TGCAGTCCTG TTACAAAGAC 
     1981 GTTTGGATAA CATGAACTTC AAGTGGAGTG AACTTCGGAA AAAGTCTCTC AACATTAGGT 
     2041 CCCATTTGGA AGCCAGTTCT GACCAGTGGA AGCGTCTGCA CCTTTCTCTG CAGGAACTTC 
     2101 TGGTGTGGCT ACAGCTGAAA GATGATGAAT TAAGCCGGCA GGCACCTATT GGAGGCGACT 
     2161 TTCCAGCAGT TCAGAAGCAG AACGATGTAC ATAGGGCCTT CAAGAGGGAA TTGAAAACTA 
     2221 AAGAACCTGT AATCATGAGT ACTCTTGAGA CTGTACGAAT ATTTCTGACA GAGCAGCCTT 
     2281 TGGAAGGACT AGAGAAACTC TACCAGGAGC CCAGAGAGCT GCCTCCTGAG GAGAGAGCCC 
     2341 AGAATGTCAC TCGGCTTCTA CGAAAGCAGG CTGAGGAGGT CAATACTGAG TGGGAAAAAT 
     2401 TGAACCTGCA CTCCGCTGAC TGGCAGAGAA AAATAGATGA GACCCTTGAA AGACTCCAGG 
     2461 AACTTCAAGA GGCCACGGAT GAGCTGGACC TCAAGCTGCG CCAAGCTGAG GTGATCAAGG 
     2521 GATCCTGGCA GCCCGTGGGC GATCTCCTCA TTGACTCTCT CCAAGATCAC CTCGAGAAAG 
     2581 TCAAGGCACT TCGAGGAGAA ATTGCGCCTC TGAAAGAGAA CGTGAGCCAC GTCAATGACC 
     2641 TTGCTCGCCA GCTTACCACT TTGGGCATTC AGCTCTCACC GTATAACCTC AGCACTCTGG 
     2701 AAGACCTGAA CACCAGATGG AAGCTTCTGC AGGTGGCCGT CGAGGACCGA GTCAGGCAGC 
     2761 TGCATGAAGC CCACAGGGAC TTTGGTCCAG CATCTCAGCA CTTTCTTTCC ACGTCTGTCC 
     2821 AGGGTCCCTG GGAGAGAGCC ATCTCGCCAA ACAAAGTGCC CTACTATATC AACCACGAGA 
     2881 CTCAAACAAC TTGCTGGGAC CATCCCAAAA TGACAGAGCT CTACCAGTCT TTAGCTGACC 
     2941 TGAATAATGT CAGATTCTCA GCTTATAGGA CTGCCATGAA ACTCCGAAGA CTGCAGAAGG 
     3001 CCCTTTGCTT GGATCTCTTG AGCCTGTCAG CTGCATGTGA TGCCTTGGAC CAGCACAACC 
     3061 TCAAGCAAAA TGACCAGCCC ATGGATATCC TGCAGATTAT TAATTGTTTG ACCACTATTT 
     3121 ATGACCGCCT GGAGCAAGAG CACAACAATT TGGTCAACGT CCCTCTCTGC GTGGATATGT 
     3181 GTCTGAACTG GCTGCTGAAT GTTTATGATA CGGGACGAAC AGGGAGGATC CGTGTCCTGT 
     3241 CTTTTAAAAC TGGCATCATT TCCCTGTGTA AAGCACATTT GGAAGACAAG TACAGATACC 
     3301 TTTTCAAGCA AGTGGCAAGT TCAACAGGAT TTTGTGACCA GCGCAGGCTG GGCCTCCTTC 
     3361 TGCATGATTC TATCCAAATT CCAAGACAGT TGGGTGAAGT TGCATCCTTT GGGGGCAGTA 
     3421 ACATTGAGCC AAGTGTCCGG AGCTGCTTCC AATTTGCTAA TAATAAGCCA GAGATCGAAG 
     3481 CGGCCCTCTT CCTAGACTGG ATGAGACTGG AACCCCAGTC CATGGTGTGG CTGCCCGTCC 
     3541 TGCACAGAGT GGCTGCTGCA GAAACTGCCA AGCATCAGGC CAAATGTAAC ATCTGCAAAG 
     3601 AGTGTCCAAT CATTGGATTC AGGTACAGGA GTCTAAAGCA CTTTAATTAT GACATCTGCC 
     3661 AAAGCTGCTT TTTTTCTGGT CGAGTTGCAA AAGGCCATAA AATGCACTAT CCCATGGTGG 
     3721 AATATTGCAC TCCGACTACA TCAGGAGAAG ATGTTCGAGA CTTTGCCAAG GTACTAAAAA 
     3781 ACAAATTTCG AACCAAAAGG TATTTTGCGA AGCATCCCCG AATGGGCTAC CTGCCAGTGC 
     3841 AGACTGTCTT AGAGGGGGAC AACATGGAAA CTCCCGTTAC TCTGATCAAC TTCTGGCCAG 
     3901 TAGATTCTGC GCCTGCCTCG TCCCCTCAGC TTTCACACGA TGATACTCAT TCACGCATTG 
     3961 AACATTATGC TAGCAGGCTA GCAGAAATGG AAAACAGCAA TGGATCTTAT CTAAATGATA 
     4021 GCATCTCTCC TAATGAGAGC ATAGATGATG AACATTTGTT AATCCAGCAT TACTGCCAAA 
     4081 GTTTGAACCA GGACTCCCCC CTGAGCCAGC CTCGTAGTCC TGCCCAGATC TTGATTTCCT 
     4141 TAGAGAGTGA GGAAAGAGGG GAGCTAGAGA GAATCCTAGC AGATCTTGAG GAAGAAAACA 
     4201 GGAATCTGCA AGCAGAATAT GACCGTCTAA AGCAGCAGCA CGAACATAAA GGCCTGTCCC 
     4261 CACTGCCGTC CCCTCCTGAA ATGATGCCCA CCTCTCCCCA GAGTCCCCGG GATGCTGAGC 
     4321 TCATTGCTGA GGCCAAGCTA CTGCGTCAAC ACAAAGGCCG CCTGGAAGCC AGGATGCAAA 
     4381 TCCTGGAAGA CCACAATAAA CAGCTGGAGT CACAGTTACA CAGGCTAAGG CAGCTGCTGG 
     4441 AGCAACCCCA GGCAGAGGCC AAAGTGAATG GCACAACGGT GTCCTCTCCT TCTACCTCTC 
     4501 TACAGAGGTC CGACAGCAGT CAGCCTATGC TGCTCCGAGT GGTTGGCAGT CAAACTTCGG 
     4561 ACTCCATGGG TGAGGAAGAT CTTCTCAGTC CTCCCCAGGA CACAAGCACA GGGTTAGAGG 
     4621 AGGTGATGGA GCAACTCAAC AACTCCTTCC CTAGTTCAAG AGGACACAAT GTAGGAAGTC 
     4681 TTTTCCACAT GGCAGATGAT TTGGGCAGAG CGATGGAGTC CTTAGTATCA GTCATGACAG 
     4741 ATGAAGAAGG AGCAGAAACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4801 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4861 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4921 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4981 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5041 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5101 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5161 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5221 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5281 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5341 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5401 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5461 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5521 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5581 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5641 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5701 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5761 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5821 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5881 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5941 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6001 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6061 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6121 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6181 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6241 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6301 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6361 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6421 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6481 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6541 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6601 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6661 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6721 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6781 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6841 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6901 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6961 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7021 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7081 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7141 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7201 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7261 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7321 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7381 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7441 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7501 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7561 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7621 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7681 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7741 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7801 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7861 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7921 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7981 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8041 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8101 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8161 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8221 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8281 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8341 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8401 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8461 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8521 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8581 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//