LOCUS       RC213878                8343 bp    DNA     circular  SYN 
DEFINITION  JAK1 (Myc-DDK-tagged)-Human Janus kinase 1 (JAK1)
ACCESSION   NM_002227
VERSION     NM_002227.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4500..4591 
                     /label="Myc-DDK"  
     misc_feature    4621..5206 
                     /label="PolyA signal"  
     misc_feature    4678..4697 
                     /label="Restriction Enzyme"  
     misc_feature    5482..6154 
                     /label="ColE1"  
     misc_feature    6584..7378 
                     /label="Kan/Neo"  
     misc_feature    7413..7715 
                     /label="SV40 ori"  
     misc_feature    7801..8278 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4482..4488 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4482 
                     /label="JAK1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGTATCTA AATATAAAAG AGGACTGCAA TGCCATGGCT TTCTGTGCTA 
     1081 AAATGAGGAG CTCCAAGAAG ACTGAGGTGA ACCTGGAGGC CCCTGAGCCA GGGGTGGAAG 
     1141 TGATCTTCTA TCTGTCGGAC AGGGAGCCCC TCCGGCTGGG CAGTGGAGAG TACACAGCAG 
     1201 AGGAACTGTG CATCAGGGCT GCACAGGCAT GCCGTATCTC TCCTCTTTGT CACAACCTCT 
     1261 TTGCCCTGTA TGACGAGAAC ACCAAGCTCT GGTATGCTCC AAATCGCACC ATCACCGTTG 
     1321 ATGACAAGAT GTCCCTCCGG CTCCACTACC GGATGAGGTT CTATTTCACC AATTGGCATG 
     1381 GAACCAACGA CAATGAGCAG TCAGTGTGGC GTCATTCTCC AAAGAAGCAG AAAAATGGCT 
     1441 ACGAGAAAAA AAAGATTCCA GATGCAACCC CTCTCCTTGA TGCCAGCTCA CTGGAGTATC 
     1501 TGTTTGCTCA GGGACAGTAT GATTTGGTGA AATGCCTGGC TCCTATTCGA GACCCCAAGA 
     1561 CCGAGCAGGA TGGACATGAT ATTGAGAACG AGTGTCTAGG GATGGCTGTC CTGGCCATCT 
     1621 CACACTATGC CATGATGAAG AAGATGCAGT TGCCAGAACT GCCCAAGGAC ATCAGCTACA 
     1681 AGCGATATAT TCCAGAAACA TTGAATAAGT CCATCAGACA GAGGAACCTT CTCACCAGGA 
     1741 TGCGGATAAA TAATGTTTTC AAGGATTTCC TAAAGGAATT TAACAACAAG ACCATTTGTG 
     1801 ACAGCAGCGT GTCCACGCAT GACCTGAAGG TGAAATACTT GGCTACCTTG GAAACTTTGA 
     1861 CAAAACATTA CGGTGCTGAA ATATTTGAGA CTTCCATGTT ACTGATTTCA TCAGAAAATG 
     1921 AGATGAATTG GTTTCATTCG AATGACGGTG GAAACGTTCT CTACTACGAA GTGATGGTGA 
     1981 CTGGGAATCT TGGAATCCAG TGGAGGCATA AACCAAATGT TGTTTCTGTT GAAAAGGAAA 
     2041 AAAATAAACT GAAGCGGAAA AAACTGGAAA ATAAACACAA GAAGGATGAG GAGAAAAACA 
     2101 AGATCCGGGA AGAGTGGAAC AATTTTTCTT ACTTCCCTGA AATCACTCAC ATTGTAATAA 
     2161 AGGAGTCTGT GGTCAGCATT AACAAGCAGG ACAACAAGAA AATGGAACTG AAGCTCTCTT 
     2221 CCCACGAGGA GGCCTTGTCC TTTGTGTCCC TGGTAGATGG CTACTTCCGG CTCACAGCAG 
     2281 ATGCCCATCA TTACCTCTGC ACCGACGTGG CCCCCCCGTT GATCGTCCAC AACATACAGA 
     2341 ATGGCTGTCA TGGTCCAATC TGTACAGAAT ACGCCATCAA TAAATTGCGG CAAGAAGGAA 
     2401 GCGAGGAGGG GATGTACGTG CTGAGGTGGA GCTGCACCGA CTTTGACAAC ATCCTCATGA 
     2461 CCGTCACCTG CTTTGAGAAG TCTGAGCAGG TGCAGGGTGC CCAGAAGCAG TTCAAGAACT 
     2521 TTCAGATCGA GGTGCAGAAG GGCCGCTACA GTCTGCACGG TTCGGACCGC AGCTTCCCCA 
     2581 GCTTGGGAGA CCTCATGAGC CACCTCAAGA AGCAGATCCT GCGCACGGAT AACATCAGCT 
     2641 TCATGCTAAA ACGCTGCTGC CAGCCCAAGC CCCGAGAAAT CTCCAACCTG CTGGTGGCTA 
     2701 CTAAGAAAGC CCAGGAGTGG CAGCCCGTCT ACCCCATGAG CCAGCTGAGT TTCGATCGGA 
     2761 TCCTCAAGAA GGATCTGGTG CAGGGCGAGC ACCTTGGGAG AGGCACGAGA ACACACATCT 
     2821 ATTCTGGGAC CCTGATGGAT TACAAGGATG ACGAAGGAAC TTCTGAAGAG AAGAAGATAA 
     2881 AAGTGATCCT CAAAGTCTTA GACCCCAGCC ACAGGGATAT TTCCCTGGCC TTCTTCGAGG 
     2941 CAGCCAGCAT GATGAGACAG GTCTCCCACA AACACATCGT GTACCTCTAT GGCGTCTGTG 
     3001 TCCGCGACGT GGAGAATATC ATGGTGGAAG AGTTTGTGGA AGGGGGTCCT CTGGATCTCT 
     3061 TCATGCACCG GAAAAGCGAT GTCCTTACCA CACCATGGAA ATTCAAAGTT GCCAAACAGC 
     3121 TGGCCAGTGC CCTGAGCTAC TTGGAGGATA AAGACCTGGT CCATGGAAAT GTGTGTACTA 
     3181 AAAACCTCCT CCTGGCCCGT GAGGGCATCG ACAGTGAGTG TGGCCCATTC ATCAAGCTCA 
     3241 GTGACCCCGG CATCCCCATT ACGGTGCTGT CTAGGCAAGA ATGCATTGAA CGAATCCCAT 
     3301 GGATTGCTCC TGAGTGTGTT GAGGACTCCA AGAACCTGAG TGTGGCTGCT GACAAGTGGA 
     3361 GCTTTGGAAC CACGCTCTGG GAAATCTGCT ACAATGGCGA GATCCCCTTG AAAGACAAGA 
     3421 CGCTGATTGA GAAAGAGAGA TTCTATGAAA GCCGGTGCAG GCCAGTGACA CCATCATGTA 
     3481 AGGAGCTGGC TGACCTCATG ACCCGCTGCA TGAACTATGA CCCCAATCAG AGGCCTTTCT 
     3541 TCCGAGCCAT CATGAGAGAC ATTAATAAGC TTGAAGAGCA GAATCCAGAT ATTGTTTCAG 
     3601 AAAAAAAACC AGCAACTGAA GTGGACCCCA CACATTTTGA AAAGCGCTTC CTAAAGAGGA 
     3661 TCCGTGACTT GGGAGAGGGC CACTTTGGGA AGGTTGAGCT CTGCAGGTAT GACCCCGAAG 
     3721 GGGACAATAC AGGGGAGCAG GTGGCTGTTA AATCTCTGAA GCCTGAGAGT GGAGGTAACC 
     3781 ACATAGCTGA TCTGAAAAAG GAAATCGAGA TCTTAAGGAA CCTCTATCAT GAGAACATTG 
     3841 TGAAGTACAA AGGAATCTGC ACAGAAGACG GAGGAAATGG TATTAAGCTC ATCATGGAAT 
     3901 TTCTGCCTTC GGGAAGCCTT AAGGAATATC TTCCAAAGAA TAAGAACAAA ATAAACCTCA 
     3961 AACAGCAGCT AAAATATGCC GTTCAGATTT GTAAGGGGAT GGACTATTTG GGTTCTCGGC 
     4021 AATACGTTCA CCGGGACTTG GCAGCAAGAA ATGTCCTTGT TGAGAGTGAA CACCAAGTGA 
     4081 AAATTGGAGA CTTCGGTTTA ACCAAAGCAA TTGAAACCGA TAAGGAGTAT TACACCGTCA 
     4141 AGGATGACCG GGACAGCCCT GTGTTTTGGT ATGCTCCAGA ATGTTTAATG CAATCTAAAT 
     4201 TTTATATTGC CTCTGACGTC TGGTCTTTTG GAGTCACTCT GCATGAGCTG CTGACTTACT 
     4261 GTGATTCAGA TTCTAGTCCC ATGGCTTTGT TCCTGAAAAT GATAGGCCCA ACCCATGGCC 
     4321 AGATGACAGT CACAAGACTT GTGAATACGT TAAAAGAAGG AAAACGCCTG CCGTGCCCAC 
     4381 CTAACTGTCC AGATGAGGTT TATCAACTTA TGAGGAAATG CTGGGAATTC CAACCATCCA 
     4441 ATCGGACAAG CTTTCAGAAC CTTATTGAAG GATTTGAAGC ACTTTTAAAA ACGCGTACGC 
     4501 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4561 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4621 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4681 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4741 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4801 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4861 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4921 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4981 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5041 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5101 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5161 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5221 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5281 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5341 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5401 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5461 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5521 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5581 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5641 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5701 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5761 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5821 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5881 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5941 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6001 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6061 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6121 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6181 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6241 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6301 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6361 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6421 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6481 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6541 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6601 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6661 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6721 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6781 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6841 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6901 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6961 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7021 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7081 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7141 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7201 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7261 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7321 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7381 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7441 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7501 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7561 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7621 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7681 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7741 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7801 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7861 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7921 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7981 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8041 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8101 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8161 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8221 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8281 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8341 TTT
//