LOCUS       RC213847                9261 bp    DNA     circular  SYN 
DEFINITION  PTPRT (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, T (PTPRT), transcript variant 1
ACCESSION   NM_133170
VERSION     NM_133170.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5418..5509 
                     /label="Myc-DDK"  
     misc_feature    5539..6124 
                     /label="PolyA signal"  
     misc_feature    5596..5615 
                     /label="Restriction Enzyme"  
     misc_feature    6400..7072 
                     /label="ColE1"  
     misc_feature    7502..8296 
                     /label="Kan/Neo"  
     misc_feature    8331..8633 
                     /label="SV40 ori"  
     misc_feature    8719..9196 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5400..5406 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5400 
                     /label="PTPRT"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAGCCTC GCCGCGCTCG CCCTCAGCCT GCTCCTGAGG CTGCAGCTGC 
     1081 CGCCACTGCC CGGCGCCCGG GCTCAGAGCG CCGCAGGTGG CTGTTCCTTT GATGAGCACT 
     1141 ACAGCAACTG TGGTTATAGT GTGGCTCTAG GGACCAATGG GTTCACCTGG GAGCAGATTA 
     1201 ACACATGGGA GAAACCAATG CTGGACCAGG CAGTGCCCAC AGGATCTTTC ATGATGGTGA 
     1261 ACAGCTCTGG GAGAGCCTCT GGCCAGAAGG CCCACCTTCT CCTGCCAACC CTGAAGGAGA 
     1321 ATGACACCCA CTGCATCGAC TTCCATTACT ACTTCTCCAG CCGTGACAGG TCCAGCCCAG 
     1381 GGGCCTTGAA CGTCTACGTG AAGGTGAATG GTGGCCCCCA AGGGAACCCT GTGTGGAATG 
     1441 TGTCCGGGGT CGTCACTGAG GGCTGGGTGA AGGCAGAGCT CGCCATCAGC ACTTTCTGGC 
     1501 CACATTTCTA TCAGGTGATA TTTGAATCCG TCTCATTGAA GGGTCATCCT GGCTACATCG 
     1561 CCGTGGACGA GGTCCGGGTC CTTGCTCATC CATGCAGAAA AGCACCTCAT TTTCTGCGAC 
     1621 TCCAAAACGT GGAGGTGAAT GTGGGGCAGA ATGCCACATT TCAGTGCATT GCTGGTGGGA 
     1681 AGTGGTCTCA GCATGACAAG CTTTGGCTCC AGCAATGGAA TGGCAGGGAC ACGGCCCTGA 
     1741 TGGTCACCCG TGTGGTCAAC CACAGGCGCT TCTCAGCCAC AGTCAGTGTG GCAGACACTG 
     1801 CCCAGCGGAG CGTCAGCAAG TACCGCTGTG TGATCCGCTC TGATGGTGGG TCTGGTGTGT 
     1861 CCAACTACGC GGAGCTGATC GTGAAAGAGC CTCCCACGCC CATTGCTCCC CCAGAGCTGC 
     1921 TGGCTGTGGG GGCCACATAC CTGTGGATCA AGCCAAATGC CAACTCCATC ATCGGGGATG 
     1981 GCCCCATCAT CCTGAAGGAA GTGGAATATC GCACCACCAC AGGCACGTGG GCAGAGACCC 
     2041 ACATAGTCGA CTCTCCCAAC TATAAGCTGT GGCATCTGGA CCCCGATGTT GAGTATGAGA 
     2101 TCCGAGTGCT CCTCACACGA CCAGGTGAGG GGGGTACGGG ACCGCCAGGG CCTCCCCTCA 
     2161 CCACCAGGAC CAAGTGTGCA GATCCGGTAC ATGGCCCACA GAACGTGGAA ATCGTAGACA 
     2221 TCAGAGCCCG GCAGCTGACC CTGCAGTGGG AGCCCTTCGG CTACGCGGTG ACCCGCTGCC 
     2281 ATAGCTACAA CCTCACCGTG CAGTACCAGT ATGTGTTCAA CCAGCAGCAG TACGAGGCCG 
     2341 AGGAGGTCAT CCAGACCTCC TCCCACTACA CCCTGCGAGG CCTGCGCCCC TTCATGACCA 
     2401 TCCGGCTGCG ACTCTTGCTG TCTAACCCCG AGGGCCGAAT GGAGAGCGAG GAGCTGGTGG 
     2461 TGCAGACTGA GGAAGACGTT CCAGGAGCTG TTCCTCTAGA ATCCATCCAA GGGGGGCCCT 
     2521 TTGAGGAGAA GATCTACATC CAGTGGAAAC CTCCCAATGA GACCAATGGG GTCATCACGC 
     2581 TCTACGAGAT CAACTACAAG GCTGTCGGCT CGCTGGACCC AAGTGCTGAC CTCTCGAGCC 
     2641 AGAGGGGGAA AGTGTTCAAG CTCCGGAATG AAACCCACCA CCTCTTTGTG GGTCTGTACC 
     2701 CAGGGACCAC CTATTCCTTC ACCATCAAGG CCAGCACAGC AAAGGGCTTT GGGCCCCCTG 
     2761 TCACCACTCG GATTGCCACC AAAATTTCAG CTCCATCCAT GCCTGAGTAC GACACAGACA 
     2821 CCCCATTGAA TGAGACAGAC ACGACCATCA CAGTGATGCT GAAACCCGCT CAGTCCCGGG 
     2881 GAGCTCCTGT CAGTGTTTAT CAGCTGGTTG TCAAGGAGGA GCGACTTCAG AAGTCACGGA 
     2941 GGGCAGCTGA CATTATTGAG TGCTTTTCGG TGCCCGTGAG CTATCGGAAT GCCTCCAGCC 
     3001 TCGATTCTCT ACACTACTTT GCTGCTGAGT TGAAGCCTGC CAACCTGCCT GTCACCCAGC 
     3061 CATTTACAGT GGGTGACAAT AAGACATACA ATGGCTACTG GAACCCTCCT CTCTCTCCCC 
     3121 TGAAAAGCTA CAGCATCTAC TTCCAGGCAC TCAGCAAAGC CAATGGAGAG ACCAAAATCA 
     3181 ACTGTGTTCG TCTGGCTACA AAAGCACCAA TGGGCAGCGC CCAGGTGACC CCGGGGACTC 
     3241 CACTCTGCCT CCTCACCACA GGTGCCTCCA CCCAGAATTC TAACACTGTG GAGCCAGAGA 
     3301 AGCAGGTGGA CAACACCGTG AAGATGGCTG GCGTGATCGC TGGCCTCCTC ATGTTCATCA 
     3361 TCATTCTCCT GGGCGTGATG CTCACCATCA AAAGGAGAAG AAATGCTTAT TCCTACTCCT 
     3421 ATTACTTGAA GCTGGCCAAG AAGCAGAAGG AGACCCAGAG TGGAGCCCAG AGGGAGATGG 
     3481 GGCCTGTGGC CTCTGCCGAC AAACCCACCA CCAAGCTCAG CGCCAGCCGC AATGATGAAG 
     3541 GCTTCTCTTC TAGTTCTCAG GACGTCAACG GATTCACAGA TGGCAGCCGC GGGGAGCTTT 
     3601 CCCAGCCCAC CCTCACGATC CAGACTCATC CCTACCGCAC CTGTGACCCT GTGGAGATGA 
     3661 GCTACCCCCG GGACCAGTTC CAACCCGCCA TCCGGGTGGC TGACTTGCTG CAGCACATCA 
     3721 CGCAGATGAA GAGAGGCCAG GGCTACGGGT TCAAGGAGGA ATACGAGGCC TTACCAGAGG 
     3781 GGCAGACAGC TTCGTGGGAC ACAGCCAAGG AGGATGAAAA CCGCAATAAG AATCGATATG 
     3841 GGAACATCAT ATCCTACGAC CATTCCCGGG TGAGGCTGCT GGTGCTGGAT GGAGACCCGC 
     3901 ACTCTGACTA CATCAATGCC AACTACATTG ACGGATACCA TCGACCTCGG CACTACATTG 
     3961 CGACTCAAGG TCCGATGCAG GAGACTGTAA AGGACTTTTG GAGAATGATC TGGCAGGAGA 
     4021 ACTCCGCCAG CATCGTCATG GTCACAAACC TGGTGGAAGT GGGCAGGGTG AAATGTGTGC 
     4081 GATACTGGCC AGATGACACG GAGGTCTACG GAGACATTAA AGTCACCCTG ATTGAAACAG 
     4141 AGCCCCTGGC AGAATACGTC ATACGCACCT TCACAGTCCA GAAGAAAGGC TACCATGAGA 
     4201 TCCGGGAGCT CCGCCTCTTC CACTTCACCA GCTGGCCTGA CCACGGCGTT CCCTGCTATG 
     4261 CCACTGGCCT TCTGGGCTTC GTCCGCCAGG TCAAGTTCCT CAACCCCCCG GAAGCTGGGC 
     4321 CCATAGTGGT CCACTGCAGT GCTGGGGCTG GGCGGACTGG CTGCTTCATT GCCATTGACA 
     4381 CCATGCTTGA CATGGCCGAG AATGAAGGGG TGGTGGACAT CTTCAACTGC GTGCGTGAGC 
     4441 TCCGGGCCCA AAGGGTCAAC CTGGTACAGA CAGAGGAGCA ATATGTGTTT GTGCACGATG 
     4501 CCATCCTGGA AGCGTGCCTC TGTGGCAACA CTGCCATCCC TGTGTGTGAG TTCCGTTCTC 
     4561 TCTACTACAA TATCAGCAGG CTGGACCCCC AGACAAACTC CAGCCAAATC AAAGATGAAT 
     4621 TTCAGACCCT CAACATTGTG ACACCCCGTG TGCGGCCCGA GGACTGCAGC ATTGGGCTCC 
     4681 TGCCCCGGAA CCATGATAAG AATCGAAGTA TGGACGTGCT GCCTCTGGAC CGCTGCCTGC 
     4741 CCTTCCTTAT CTCAGTGGAC GGAGAATCCA GCAATTACAT CAACGCAGCA CTGATGGATA 
     4801 GCCACAAGCA GCCTGCCGCC TTCGTGGTCA CCCAGCACCC TCTACCCAAC ACCGTGGCAG 
     4861 ACTTCTGGAG GCTGGTGTTC GATTACAACT GCTCCTCTGT GGTGATGCTG AATGAGATGG 
     4921 ACACTGCCCA GTTCTGTATG CAGTACTGGC CTGAGAAGAC CTCCGGGTGC TATGGGCCCA 
     4981 TCCAGGTGGA GTTCGTCTCC GCAGACATCG ACGAGGACAT CATCCACAGA ATATTCCGCA 
     5041 TCTGTAACAT GGCCCGGCCA CAGGATGGTT ATCGTATAGT CCAGCACCTC CAGTACATTG 
     5101 GCTGGCCTGC CTACCGGGAC ACGCCCCCCT CCAAGCGCTC TCTGCTCAAA GTGGTCCGAC 
     5161 GACTGGAGAA GTGGCAGGAG CAGTATGACG GGAGGGAGGG ACGTACTGTG GTCCACTGCC 
     5221 TAAATGGGGG AGGCCGTAGT GGAACCTTCT GTGCCATCTG CAGTGTGTGT GAGATGATCC 
     5281 AGCAGCAAAA CATCATTGAC GTGTTCCACA TCGTGAAAAC ACTGCGTAAC AACAAATCCA 
     5341 ACATGGTGGA GACCCTGGAA CAGTATAAAT TTGTATACGA GGTGGCACTG GAATATTTAA 
     5401 GCTCCTTTAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     5461 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5521 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5581 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5641 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5701 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5761 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5821 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5881 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5941 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     6001 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     6061 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     6121 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     6181 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     6241 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     6301 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     6361 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     6421 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6481 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6541 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6601 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6661 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6721 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6781 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6841 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6901 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6961 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     7021 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     7081 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     7141 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     7201 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     7261 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     7321 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     7381 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     7441 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7501 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7561 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7621 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7681 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7741 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7801 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7861 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7921 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7981 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     8041 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     8101 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     8161 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     8221 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     8281 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     8341 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     8401 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     8461 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8521 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8581 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8641 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8701 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8761 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8821 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8881 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8941 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     9001 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     9061 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     9121 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     9181 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     9241 CAAAAATTTA ACGCGAATTT T
//