LOCUS       RC213814                8457 bp    DNA     circular  SYN 
DEFINITION  WWC2 (Myc-DDK-tagged)-Human WW and C2 domain containing 2 (WWC2)
ACCESSION   NM_024949
VERSION     NM_024949.5
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4614..4705 
                     /label="Myc-DDK"  
     misc_feature    4735..5320 
                     /label="PolyA signal"  
     misc_feature    4792..4811 
                     /label="Restriction Enzyme"  
     misc_feature    5596..6268 
                     /label="ColE1"  
     misc_feature    6698..7492 
                     /label="Kan/Neo"  
     misc_feature    7527..7829 
                     /label="SV40 ori"  
     misc_feature    7915..8392 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4596..4602 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4596 
                     /label="WWC2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTAGGAGG GCCGGGAGCG GTCAGCTGCC GCTGCCCCGG GGCTGGGAGG 
     1081 AGGCCAGGGA CTACGACGGC AAGGTCTTCT ACATTGACCA CAACACCAGG AGGACCAGCT 
     1141 GGATCGACCC CCGGGACAGG TTAACGAAGC CCTTGTCATT TGCTGATTGT GTTGGGGATG 
     1201 AGCTGCCGTG GGGATGGGAA GCAGGGTTTG ACCCTCAGAT TGGTGTCTAC TACATCGATC 
     1261 ACATCAACAA AACCACGCAG ATAGAAGATC CAAGAAAACA ATGGAGGGGG GAACAGGAGA 
     1321 AGATGCTCAA GGACTACCTC TCTGTGGCAC AGGATGCCCT CCGGACACAG AAGGAACTGT 
     1381 ACCATGTGAA GGAGCAGAGG CTGGCGCTGG CCCTGGATGA ATACGTGCGA TTAAATGATG 
     1441 CCTATAAGGA AAAGTCAAGT TCTCACACAA GCTTATTCTC AGGATCTTCA TCCAGTACTA 
     1501 AATATGATCC CGATATTTTA AAAGCTGAGA TCTCCACTAC AAGATTAAGG GTTAAAAAGC 
     1561 TAAAGAGAGA GCTCTCACAG ATGAAGCAGG AACTGCTCTA TAAAGAACAA GGCTTTGAAA 
     1621 CATTGCAGCA AATTGATAAA AAAATGTCTG GAGGCCAGAG CGGGTATGAA CTCAGTGAAG 
     1681 CCAAAGCCAT TCTAACAGAA CTAAAATCTA TCAGAAAGGC AATTAGCTCA GGAGAAAAAG 
     1741 AAAAACAAGA TCTGATGCAG AGTCTTGCTA AGCTGCAGGA GCGGTTTCAT TTGGATCAGA 
     1801 ACATTGGCAG ATCTGAGCCA GATTTGAGAT GTAGTCCTGT GAACTCTCAT TTATGTCTCT 
     1861 CCAGACAGAC CCTTGATGCT GGGTCACAAA CAAGCATTTC CGGAGATATT GGAGTAAGAA 
     1921 GTAGATCAAA TTTAGCTGAA AAGGTCAGGC TAAGCCTACA GTATGAAGAA GCCAAAAGAA 
     1981 GTATGGCCAA CTTAAAAATT GAACTGTCAA AATTGGACAG TGAGGCCTGG CCTGGGGCAC 
     2041 TGGATATTGA GAAGGAAAAA CTGATGCTGA TTAATGAAAA AGAAGAACTT TTGAAAGAGC 
     2101 TTCAGTTCGT CACCCCACAG AAACGTACCC AAGATGAATT AGAACGCCTA GAAGCTGAAA 
     2161 GGCAGCGGCT GGAAGAAGAG TTGCTGTCTG TGAGGGGAAC ACCAAGCAGA GCTCTGGCCG 
     2221 AGAGATTGAG ATTGGAAGAG AGAAGAAAAG AGCTGCTACA GAAACTTGAA GAAACTACTA 
     2281 AATTAACTAC TTATTTGCAT TCACAACTTA AAAGCCTCTC TGCCAGCACC CTGTCCATGT 
     2341 CATCTGGGAG CAGCCTGGGT TCCCTGGCAT CGAGTCGGGG CTCTCTGAAC ACCTCCAGCA 
     2401 GAGGGTCACT CAACTCCCTC AGTTCCACCG AACTCTATTA CAGCAGTCAA AGTGATCAGA 
     2461 TAGATGTGGA TTATCAGTAT AAACTGGACT TCCTTCTGCA AGAGAAAAGC GGTTACATTC 
     2521 CTTCTGGACC CATCACCACC ATCCATGAAA ACGAGGTGGT CAAGTCCCCT AGCCAGCCTG 
     2581 GCCAGAGTGG ACTCTGTGGA GTGGCAGCTG CAGCAACAGG CCACACTCCT CCACTGGCTG 
     2641 AGGCCCCGAA GTCTGTGGCC TCCCTGTCCT CGAGGTCCTC CCTTTCCTCC TTGTCTCCTC 
     2701 CAGGCTCTCC CTTGGTTTTG GAAGGCACGT TTCCCATGTC TTCTTCTCAT GATGCCTCTC 
     2761 TCCATCAGTT CACTGCTGAC TTTGAAGACT GTGAGTTGAG TAGCCATTTT GCAGATATCA 
     2821 GCCTCATCGA AAATCAGATT TTGCTGGATT CTGATTCAGG AGGAGCCTCC CAGTCTCTTT 
     2881 CAGAGGATAA AGACCTTAAT GAATGTGCTA GGGAGCCATT ATATGAAGGA ACTGCAGATG 
     2941 TGGAAAAATC ATTACCAAAA AGAAGAGTGA TCCACTTGCT TGGGGAGAAA ACCACTTGTG 
     3001 TGTCGGCTGC TGTGTCTGAT GAGTCTGTGG CTGGAGACAG TGGGGTCTAT GAAGCTTTCG 
     3061 TGAAACAACC TAGTGAAATG GAAGATGTCA CATACAGTGA AGAGGATGTA GCCATTGTAG 
     3121 AGACCGCCCA GGTTCAGATA GGACTCAGAT ACAATGCAAA AAGTTCAAGT TTCATGGTGA 
     3181 TTATAGCACA GCTCCGAAAC CTTCATGCCT TCTTGATACC TCATACTTCA AAAGTATATT 
     3241 TTAGGGTTGC CGTTCTTCCT TCCTCAACTG ATGTCAGCTG TCTGTTTCGC ACAAAAGTTC 
     3301 ATCCGCCCAC AGAATCCATT TTATTCAATG ATGTGTTCAG AGTCGCCATT TCCCAAACAG 
     3361 CCTTACAACA GAAGACACTG AGGGTAGACC TTTGCTCTGT CAGTAAACAC CGAAGGGAAG 
     3421 AATGCCTGGC TGGAACTCAG ATCAGCCTGG CAGATTTACC ATTTTCCAGT GAGGTTTTCA 
     3481 CTCTATGGTA TAACTTGCTT CCTTCCAAGC AAATGCCTTG CAAAAAGAAT GAAGAAAATG 
     3541 AGGACTCTGT ATTTCAACCA AACCAGCCGT TAGTAGATTC TATAGACTTG GATGCAGTGT 
     3601 CAGCCTTACT TGCAAGAACA TCAGCTGAGT TGTTAGCTGT GGAACAAGAA TTAGCACAAG 
     3661 AAGAAGAAGA AGAATCAGGA CAAGAAGAGC CAAGGGGCCC AGATGGAGAC TGGCTAACAA 
     3721 TGCTAAGAGA GGCCTCTGAT GAAATTGTGG CTGAAAAAGA GGCTGAAGTT AAATTGCCAG 
     3781 AGGACAGTAG CTGTACAGAA GATTTAAGTT CATGCACTAG TGTGCCTGAG ATGAATGAAG 
     3841 ACGGGAACAG GAAAGAAAGC AACTGTGCCA AAGACCTCAG AAGTCAGCCA CCTACTAGAA 
     3901 TACCAACACT GGTTGACAAA GAGACAAACA CTGATGAAGC CGCTAATGAC AATATGGCAG 
     3961 TTCGCCCCAA AGAGCGCAGC AGCCTGAGCT CTAGACAGCA TCCGTTTGTG AGGAGCAGTG 
     4021 TGATAGTGCG CTCACAGACC TTTTCTCCAG GAGAGCGGAA CCAGTACATC TGCAGGTTAA 
     4081 ATCGGAGTGA CAGTGACAGT TCAACCCTGG CTAAAAAATC ACTGTTTGTG AGAAACTCCA 
     4141 CCGAACGCCG CAGTTTGAGG GTCAAAAGGA CGGTTTGCCA GTCAGTCCTT AGAAGAACAA 
     4201 CACAGGAATG CCCAGTGCGG ACATCTCTAG ACTTAGAACT GGACCTTCAG GCATCTCTGA 
     4261 CCCGGCAGAG CCGCCTCAAT GATGAGCTGC AGGCGCTGAG GGACTTGCGG CAGAAGCTGG 
     4321 AGGAACTGAA AGCTCAGGGA GAGACTGACC TTCCACCAGG CGTGCTGGAG GATGAGAGGT 
     4381 TCCAGAGGCT TCTGAAGCAA GCTGAGAAGC AGGCTGAACA GTCCAAAGAA GAGCAGAAGC 
     4441 AAGGTCTGAA TGCAGAGAAG TTGATGAGGC AAGTCTCCAA GGACGTGTGT CGGCTCCGGG 
     4501 AGCAGAGCCA GAAGGTGCCT CGGCAGGTGC AGTCCTTCAG GGAGAAGATT GCCTACTTCA 
     4561 CCAGAGCAAA GATAAGCATC CCATCCCTGC CAGCTGATGA TGTGACGCGT ACGCGGCCGC 
     4621 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4681 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4741 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4801 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4861 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4921 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4981 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5041 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5101 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5161 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5221 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5281 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5341 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5401 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5461 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5521 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5581 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5641 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5701 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5761 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5821 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5881 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5941 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6001 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6061 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6121 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6181 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6241 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6301 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6361 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6421 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6481 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6541 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6601 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6661 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6721 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6781 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6841 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6901 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6961 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7021 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7081 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7141 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7201 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7261 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7321 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7381 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7441 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7501 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7561 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7621 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7681 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7741 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7801 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7861 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7921 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7981 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8041 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8101 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8161 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8221 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8281 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8341 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8401 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//