LOCUS       RC213737                8541 bp    DNA     circular  SYN 
DEFINITION  ATP2B3 (Myc-DDK-tagged)-Human ATPase, Ca++ transporting, plasma membrane 3 (ATP2B3), transcript variant 2
ACCESSION   NM_001001344
VERSION     NM_001001344.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4698..4789 
                     /label="Myc-DDK"  
     misc_feature    4819..5404 
                     /label="PolyA signal"  
     misc_feature    4876..4895 
                     /label="Restriction Enzyme"  
     misc_feature    5680..6352 
                     /label="ColE1"  
     misc_feature    6782..7576 
                     /label="Kan/Neo"  
     misc_feature    7611..7913 
                     /label="SV40 ori"  
     misc_feature    7999..8476 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4680..4686 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4680 
                     /label="ATP2B3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCGACATG GCCAATAGTT CCATCGAGTT CCACCCCAAG CCCCAGCAGC 
     1081 AGCGGGATGT CCCCCAGGCT GGAGGCTTTG GGTGCACGCT GGCGGAGCTG CGCACCCTCA 
     1141 TGGAGCTGCG AGGGGCCGAG GCGCTGCAGA AGATCGAGGA GGCCTACGGG GATGTCAGCG 
     1201 GGCTCTGCCG GAGGCTGAAG ACCTCACCCA CAGAGGGCCT GGCGGACAAC ACCAATGACC 
     1261 TGGAGAAGCG CAGGCAGATC TACGGGCAGA ACTTCATCCC CCCAAAGCAA CCCAAGACCT 
     1321 TCCTGCAGCT GGTGTGGGAG GCCCTGCAGG ACGTGACCCT CATCATCCTG GAGGTGGCTG 
     1381 CCATCGTCTC TCTGGGCCTC TCGTTCTATG CGCCGCCAGG AGAGGAGAGT GAAGCCTGTG 
     1441 GGAATGTGTC GGGAGGCGCA GAAGATGAGG GCGAGGCCGA AGCTGGCTGG ATCGAGGGGG 
     1501 CTGCCATCCT GCTGTCCGTC ATCTGTGTGG TGCTGGTCAC GGCCTTCAAT GACTGGAGCA 
     1561 AGGAGAAGCA GTTCCGAGGC CTGCAGAGCC GAATTGAGCA GGAGCAGAAG TTCACGGTCA 
     1621 TCCGGAACGG GCAGCTCCTC CAGGTCCCCG TGGCTGCGCT GGTGGTGGGG GACATTGCCC 
     1681 AGGTCAAGTA CGGCGACCTG CTGCCAGCCG ACGGCGTGCT CATCCAGGCC AATGACCTCA 
     1741 AGATCGACGA GAGCTCCCTG ACGGGCGAGT CTGACCACGT GCGCAAGTCA GCTGACAAAG 
     1801 ATCCCATGCT GCTCTCAGGC ACTCATGTCA TGGAAGGTTC TGGAAGAATG GTGGTGACCG 
     1861 CCGTTGGCGT GAATTCCCAG ACAGGCATCA TCTTCACGCT GCTTGGAGCT GGCGGAGAGG 
     1921 AGGAAGAGAA GAAAGATAAG AAAGGCAAGC AGCAGGATGG GGCCATGGAG AGTAGCCAGA 
     1981 CCAAAGCTAA GAAGCAGGAT GGTGCAGTGG CCATGGAGAT GCAGCCCCTG AAGAGCGCGG 
     2041 AGGGTGGGGA GATGGAGGAG CGGGAGAAGA AGAAAGCCAA CGCACCCAAA AAGGAGAAGT 
     2101 CTGTCCTTCA GGGGAAGCTC ACAAAGCTAG CCGTGCAGAT CGGGAAAGCA GGGCTGGTGA 
     2161 TGTCTGCCAT CACCGTCATC ATCCTGGTCC TCTACTTTGT GATTGAGACG TTTGTCGTGG 
     2221 AAGGCCGGAC ATGGCTGGCA GAGTGCACGC CGGTCTATGT ACAATACTTC GTGAAGTTCT 
     2281 TCATCATTGG TGTCACTGTG CTGGTCGTGG CTGTCCCAGA GGGCCTGCCT CTTGCTGTCA 
     2341 CCATCTCCTT AGCTTACTCT GTCAAGAAAA TGATGAAAGA CAACAACCTG GTGCGCCACC 
     2401 TGGATGCCTG CGAGACCATG GGCAACGCCA CAGCCATCTG CTCCGACAAG ACGGGCACGC 
     2461 TCACCACCAA CCGTATGACC GTGGTCCAGT CCTACCTAGG GGACACCCAC TACAAAGAGA 
     2521 TTCCGGCCCC CAGCGCCCTG ACCCCTAAGA TCCTCGACCT CCTGGTCCAT GCCATCTCCA 
     2581 TCAACAGTGC CTATACCACC AAAATACTAC CTCCTGAGAA GGAAGGCGCC CTCCCACGCC 
     2641 AGGTGGGCAA TAAGACGGAG TGCGCCCTGC TGGGCTTCGT CTTGGACCTG AAGCGGGACT 
     2701 TCCAGCCCGT GCGCGAGCAG ATCCCGGAAG ACAAGCTTTA CAAAGTGTAC ACCTTCAACT 
     2761 CGGTCCGCAA GTCCATGAGC ACAGTCATCC GCATGCCCGA CGGTGGCTTC CGCCTCTTCA 
     2821 GCAAGGGGGC CTCAGAGATC CTCTTGAAAA AGTGCACCAA CATCTTGAAC AGCAATGGCG 
     2881 AACTCCGGGG CTTTCGGCCT CGGGACCGGG ACGACATGGT GAGGAAGATC ATCGAGCCGA 
     2941 TGGCTTGCGA TGGCCTCCGC ACCATCTGCA TCGCCTACCG GGACTTCTCT GCAGGCCAGG 
     3001 AGCCCGACTG GGACAACGAG AATGAGGTCG TGGGTGACCT CACCTGCATA GCTGTCGTGG 
     3061 GCATTGAGGA CCCTGTGCGG CCCGAGGTCC CTGAAGCTAT CCGAAAATGC CAGCGTGCTG 
     3121 GCATCACAGT CCGCATGGTG ACTGGGGACA ACATCAACAC GGCCCGGGCC ATCGCAGCCA 
     3181 AATGCGGCAT CATCCAGCCC GGGGAGGACT TCCTGTGCCT AGAAGGGAAG GAGTTCAACC 
     3241 GGCGGATCCG CAATGAGAAA GGCGAGATAG AACAGGAGCG GCTGGACAAG GTGTGGCCCA 
     3301 AGCTGAGGGT GCTGGCCCGG TCGTCTCCCA CCGACAAGCA CACACTGGTC AAAGGGATTA 
     3361 TCGACAGCAC CACTGGTGAG CAGCGGCAGG TGGTGGCTGT GACAGGGGAT GGCACCAACG 
     3421 ATGGGCCGGC CCTCAAGAAG GCGGACGTGG GCTTCGCCAT GGGCATCGCA GGGACCGACG 
     3481 TGGCCAAGGA GGCCTCCGAC ATCATCCTGA CCGATGACAA CTTCACCAGC ATCGTCAAGG 
     3541 CAGTCATGTG GGGCCGTAAC GTCTATGACA GCATCTCCAA GTTCCTGCAG TTTCAACTGA 
     3601 CGGTCAATGT GGTGGCTGTG ATCGTGGCCT TCACAGGTGC CTGCATTACT CAGGACTCTC 
     3661 CTCTCAAAGC CGTGCAGATG TTGTGGGTGA ACTTGATCAT GGACACATTT GCCTCTCTGG 
     3721 CCCTGGCGAC GGAGCCACCC ACAGAGTCGC TGCTGCTGCG GAAGCCGTAC GGCCGCGACA 
     3781 AGCCCCTCAT CTCCCGCACC ATGATGAAGA ACATTCTGGG CCACGCCGTG TACCAGCTCG 
     3841 CCATCATCTT CACCCTGCTG TTTGTCGGGG AGCTCTTCTT CGACATCGAC AGCGGGAGGA 
     3901 ATGCGCCCCT GCACTCGCCA CCCTCAGAGC ACTACACCAT CATCTTCAAC ACGTTCGTCA 
     3961 TGATGCAGCT CTTTAACGAG ATCAACGCCC GCAAGATCCA CGGCGAGAGG AACGTGTTCG 
     4021 ACGGCATCTT CAGCAACCCC ATCTTCTGCA CCATCGTTTT GGGCACTTTC GGGATTCAGA 
     4081 TTGTCATCGT CCAGTTTGGC GGGAAGCCCT TCAGCTGCTC CCCACTATCC ACAGAACAGT 
     4141 GGCTCTGGTG CCTGTTTGTT GGTGTTGGGG AGCTGGTCTG GGGACAGGTC ATTGCCACCA 
     4201 TCCCCACCAG CCAGCTCAAG TGCCTGAAGG AAGCCGGGCA CGGGCCCGGG AAGGACGAGA 
     4261 TGACCGACGA GGAGCTGGCC GAAGGCGAGG AAGAGATCGA CCATGCCGAG CGGGAGCTCC 
     4321 GCAGGGGCCA GATCCTCTGG TTCCGGGGCC TGAACCGGAT TCAGACGCAG ATCCGGGTGG 
     4381 TGAAAGCGTT CCGTAGCTCG CTCTATGAAG GCCTGGAGAA ACCAGAATCC AAGACCTCCA 
     4441 TTCACAACTT CATGGCCACG CCCGAGTTTC TGATCAATGA CTACACCCAC AACATCCCGC 
     4501 TCATTGACGA CACGGACGTG GACGAGAACG AGGAGCGCCT CCGGGCCCCC CCGCCCCCGT 
     4561 CCCCCAACCA GAACAACAAC GCCATAGACA GCGGCATCTA CCTGACCACG CATGTCACCA 
     4621 AGTCAGCTAC CTCTTCAGTG TTTTCCTCCA GTCCCGGGAG CCCGCTCCAC AGCGTGGAGA 
     4681 CGTCCCTCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4741 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4801 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4861 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4921 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4981 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5041 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5101 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5161 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5221 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5281 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5341 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5401 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5461 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5521 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5581 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5641 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5701 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5761 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5821 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5881 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5941 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6001 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6061 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6121 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6181 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6241 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6301 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6361 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6421 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6481 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6541 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6601 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6661 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6721 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6781 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6841 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6901 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6961 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7021 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7081 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7141 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7201 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7261 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7321 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7381 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7441 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7501 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7561 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7621 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7681 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7741 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7801 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7861 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7921 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7981 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8041 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8101 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8161 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8221 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8281 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8341 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8401 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8461 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8521 CAAAAATTTA ACGCGAATTT T
//