LOCUS       RC213720                7587 bp    DNA     circular  SYN 
DEFINITION  ALDH1L1 (Myc-DDK-tagged)-Human aldehyde dehydrogenase 1 family, member L1 (ALDH1L1)
ACCESSION   NM_012190
VERSION     NM_012190.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3744..3835 
                     /label="Myc-DDK"  
     misc_feature    3865..4450 
                     /label="PolyA signal"  
     misc_feature    3922..3941 
                     /label="Restriction Enzyme"  
     misc_feature    4726..5398 
                     /label="ColE1"  
     misc_feature    5828..6622 
                     /label="Kan/Neo"  
     misc_feature    6657..6959 
                     /label="SV40 ori"  
     misc_feature    7045..7522 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3726..3732 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3726 
                     /label="ALDH1L1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGATTGCA GTGATTGGAC AGAGCCTGTT TGGCCAGGAA GTTTACTGCC 
     1081 ACCTGAGGAA GGAGGGCCAC GAAGTGGTGG GTGTGTTCAC TGTTCCAGAC AAGGATGGAA 
     1141 AGGCCGACCC CCTGGGTCTG GAAGCTGAGA AGGATGGAGT GCCGGTATTC AAGTACTCCC 
     1201 GGTGGCGTGC AAAAGGACAG GCTTTGCCTG ATGTGGTGGC AAAATACCAG GCTTTGGGGG 
     1261 CCGAGCTCAA CGTCCTGCCC TTCTGCAGCC AATTCATCCC CATGGAGATA ATCAGTGCCC 
     1321 CCCGGCATGG CTCCATCATC TATCACCCGT CACTGCTCCC TAGGCACCGA GGGGCCTCGG 
     1381 CCATCAACTG GACCCTCATT CACGGAGATA AGAAAGGGGG GTTTTCCATC TTCTGGGCGG 
     1441 ATGATGGTCT GGACACCGGA GACCTGCTGC TGCAGAAGGA GTGTGAGGTG CTCCCGGACG 
     1501 ACACCGTGAG CACGCTGTAC AACCGCTTCC TCTTCCCTGA AGGCATCAAA GGGATGGTGC 
     1561 AGGCCGTGAG GCTGATCGCT GAGGGCAAAG CCCCCAGACT CCCTCAGCCT GAGGAAGGAG 
     1621 CCACCTATGA GGGGATTCAG AAGAAGGAGA CAGCCAAGAT CAACTGGGAC CAGCCGGCAG 
     1681 AGGCCATTCA CAACTGGATC CGCGGGAACG ACAAGGTGCC GGGAGCCTGG ACAGAGGCCT 
     1741 GTGAACAGAA ACTGACATTT TTCAACTCAA CGCTGAACAC TTCAGGCCTG GTGCCCGAGG 
     1801 GAGACGCTTT GCCCATCCCA GGAGCCCATC GGCCAGGGGT GGTCACCAAA GCAGGACTCA 
     1861 TCCTCTTTGG GAATGATGAC AAAATGCTGC TGGTGAAGAA TATTCAGCTG GAGGATGGCA 
     1921 AAATGATCCT GGCCTCGAAC TTCTTTAAGG GGGCAGCCAG CAGTGTCCTT GAGCTGACAG 
     1981 AGGCAGAGCT GGTTACTGCG GAGGCTGTGC GGAGTTTTTG GCAGCGGATC CTCCCCAAAG 
     2041 TCCTGGAGGT TGAAGACTCC ACTGATTTCT TCAAGTCAGG GGCCGCGTCT GTGGACGTTG 
     2101 TGAGGCTGGT GGAGGAAGTG AAGGAGCTGT GTGATGGCCT GGAGTTAGAA AATGAAGATG 
     2161 TGTACATGGC ATCCACCTTT GGGGACTTCA TCCAGCTGTT AGTGAGGAAG CTTCGAGGGC 
     2221 ACGATGAGGA GGGCGAGTGC AGCATTGACT ACGTGGAAAT GGCAGTGAAC AAGCGCACTG 
     2281 TCCGCATGCC CCACCAGCTC TTCATTGGGG GGGAGTTCGT GGATGCCGAG GGCGCCAAGA 
     2341 CCTCTGAGAC CATCAATCCC ACCGATGGAA GTGTCATCTG CCAGGTATCC CTGGCCCAAG 
     2401 TCACCGACGT CGACAAGGCA GTGGCCGCAG CCAAGGATGC CTTTGAGAAT GGACGGTGGG 
     2461 GGAAGATCGG TGCGCGGGAC CGGGGCCGGC TGATGTACAG GTTGGCAGAT CTCATGGAGC 
     2521 AGCACCAGGA GGAGCTGGCC ACCATTGAGG CCCTGGATGC GGGTGCCGTC TACACGCTGG 
     2581 CCCTGAAGAC CCACGTGGGC ATGTCCATCC AGACCTTCCG CTACTTTGCT GGCTGGTGTG 
     2641 ACAAGATCCA GGGCTCCACC ATCCCCATCA ACCAGGCCAG ACCCAACCGC AACCTGACCT 
     2701 TGACCAGGAA GGAGCCTGTT GGGGTTTGTG GCATCATCAT CCCCTGGAAC TATCCCCTGA 
     2761 TGATGCTGTC CTGGAAGACA GCTGCCTGCC TGGCTGCCGG GAACACAGTG GTGATCAAGC 
     2821 CTGCTCAGGT GACCCCACTC ACAGCCTTGA AGTTTGCAGA GCTGACATTA AAGGCCGGCA 
     2881 TTCCCAAAGG TGTGGTTAAC GTCCTCCCAG GATCTGGCTC CCTGGTCGGC CAGAGACTCT 
     2941 CAGACCATCC TGATGTGAGG AAAATCGGGT TCACAGGCTC CACAGAGGTG GGCAAGCACA 
     3001 TCATGAAAAG CTGTGCCATA AGTAACGTGA AGAAGGTGTC CCTGGAACTG GGCGGGAAGT 
     3061 CACCCCTCAT CATCTTTGCT GACTGTGACC TCAACAAGGC TGTGCAGATG GGGATGAGTT 
     3121 CTGTTTTCTT CAACAAAGGA GAGAATTGCA TTGCAGCAGG CCGACTCTTT GTGGAGGACT 
     3181 CCATTCATGA TGAGTTCGTG CGGAGAGTGG TAGAAGAGGT GCGGAAGATG AAGGTGGGCA 
     3241 ACCCGCTGGA CAGGGACACC GACCACGGGC CGCAGAATCA CCATGCCCAC CTTGTGAAGC 
     3301 TGATGGAGTA CTGCCAGCAT GGCGTGAAGG AAGGGGCCAC ACTGGTCTGC GGCGGGAATC 
     3361 AGGTCCCTCG GCCAGGGTTC TTCTTTGAGC CAACTGTTTT CACAGACGTG GAAGACCACA 
     3421 TGTTCATAGC CAAGGAGGAG TCCTTCGGGC CTGTCATGAT CATCTCTCGG TTTGCTGATG 
     3481 GGGACTTGGA TGCCGTGCTG TCTCGGGCCA ATGCCACGGA ATTTGGCCTG GCTTCTGGTG 
     3541 TCTTCACCAG GGACATCAAC AAGGCCCTGT ATGTCAGTGA CAAGCTCCAG GCAGGCACTG 
     3601 TGTTTGTCAA CACGTACAAC AAGACCGACG TGGCCGCTCC CTTCGGAGGA TTCAAACAGT 
     3661 CTGGATTTGG CAAAGATCTA GGAGAGGCGG CTCTGAACGA GTACCTGCGG GTCAAGACAG 
     3721 TGACCTTCGA ATACACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     3781 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     3841 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     3901 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     3961 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4021 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4081 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4141 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4201 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4261 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4321 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4381 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4441 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4501 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4561 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     4621 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     4681 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     4741 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     4801 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4861 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     4921 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     4981 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5041 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5101 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5161 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5221 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5281 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5341 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5401 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5461 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5521 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     5581 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     5641 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     5701 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     5761 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     5821 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     5881 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     5941 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6001 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6061 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6121 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6181 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6241 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6301 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6361 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6421 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6481 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6541 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     6601 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     6661 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     6721 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     6781 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     6841 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6901 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6961 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7021 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7081 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7141 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7201 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7261 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7321 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7381 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7441 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7501 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7561 ATTTAACAAA AATTTAACGC GAATTTT
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