LOCUS       RC213583                6669 bp    DNA     circular  SYN 
DEFINITION  MYLK2 (Myc-DDK-tagged)-Human myosin light chain kinase 2 (MYLK2)
ACCESSION   NM_033118
VERSION     NM_033118.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2826..2917 
                     /label="Myc-DDK"  
     misc_feature    2947..3532 
                     /label="PolyA signal"  
     misc_feature    3004..3023 
                     /label="Restriction Enzyme"  
     misc_feature    3808..4480 
                     /label="ColE1"  
     misc_feature    4910..5704 
                     /label="Kan/Neo"  
     misc_feature    5739..6041 
                     /label="SV40 ori"  
     misc_feature    6127..6604 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2808..2814 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2808 
                     /label="MYLK2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGACAGAA AATGGAGCAG TTGAGCTGGG AATTCAGAAC CCATCAACAG 
     1081 ACAAGGCACC TAAAGGTCCC ACAGGTGAAA GACCCCTGGC TGCAGGGAAA GACCCTGGCC 
     1141 CCCCAGACCC AAAGAAAGCT CCGGATCCAC CCACCCTGAA GAAAGATGCC AAAGCCCCTG 
     1201 CCTCAGAGAA AGGGGATGGT ACCCTGGCCC AACCCTCAAC TAGCAGCCAA GGCCCCAAAG 
     1261 GAGAGGGTGA CAGGGGCGGG GGGCCCGCGG AGGGCAGTGC TGGGCCCCCG GCAGCCCTGC 
     1321 CCCAGCAGAC TGCGACACCT GAGACCAGCG TCAAGAAGCC CAAGGCTGAG CAGGGAGCCT 
     1381 CAGGCAGCCA GGATCCTGGA AAGCCCAGGG TGGGCAAGAA GGCAGCAGAG GGCCAAGCAG 
     1441 CAGCCAGGAG GGGCTCACCT GCCTTTCTGC ATAGCCCCAG CTGTCCTGCC ATCATCTCCA 
     1501 GTTCTGAGAA GCTGCTGGCC AAGAAGCCCC CAAGCGAGGC ATCAGAGCTC ACCTTTGAAG 
     1561 GGGTGCCCAT GACCCACAGC CCCACGGATC CCAGGCCAGC CAAGGCAGAA GAAGGAAAGA 
     1621 ACATCCTGGC AGAGAGCCAG AAGGAAGTGG GAGAGAAAAC CCCAGGCCAG GCTGGCCAGG 
     1681 CTAAGATGCA AGGGGACACC TCGAGGGGGA TTGAGTTCCA GGCTGTTCCC TCAGAGAAAT 
     1741 CCGAGGTGGG GCAGGCCCTC TGTCTCACAG CCAGGGAGGA GGACTGCTTC CAGATTTTGG 
     1801 ATGATTGCCC GCCACCTCCG GCCCCCTTCC CTCACCGCAT GGTGGAGCTG AGGACCGGGA 
     1861 ATGTCAGCAG TGAATTCAGT ATGAACTCCA AGGAGGCGCT CGGAGGTGGC AAGTTTGGGG 
     1921 CAGTCTGTAC CTGCATGGAG AAAGCCACAG GCCTCAAGCT GGCAGCCAAG GTCATCAAGA 
     1981 AACAGACTCC CAAAGACAAG GAAATGGTGT TGCTGGAGAT TGAGGTCATG AACCAGCTGA 
     2041 ACCACCGCAA TCTGATCCAG CTGTATGCAG CCATCGAGAC TCCGCATGAG ATCGTCCTGT 
     2101 TCATGGAGTA CATCGAGGGC GGAGAGCTCT TCGAGAGGAT TGTGGATGAG GACTACCATC 
     2161 TGACCGAGGT GGACACCATG GTGTTTGTCA GGCAGATCTG TGACGGGATC CTCTTCATGC 
     2221 ACAAGATGAG GGTTTTGCAC CTGGACCTCA AGCCAGAGAA CATCCTGTGT GTCAACACCA 
     2281 CCGGGCATTT GGTGAAGATC ATTGACTTTG GCCTGGCACG GAGGTATAAC CCCAACGAGA 
     2341 AGCTGAAGGT GAACTTTGGG ACCCCAGAGT TCCTGTCACC TGAGGTGGTG AATTATGACC 
     2401 AAATCTCCGA TAAGACAGAC ATGTGGAGTA TGGGGGTGAT CACCTACATG CTGCTGAGCG 
     2461 GCCTCTCCCC CTTCCTGGGA GATGATGACA CAGAGACCCT AAACAACGTT CTATCTGGCA 
     2521 ACTGGTACTT TGATGAAGAG ACCTTTGAGG CCGTATCAGA CGAGGCCAAA GACTTTGTCT 
     2581 CCAACCTCAT CGTCAAGGAC CAGAGGGCCC GGATGAACGC TGCCCAGTGT CTCGCCCATC 
     2641 CCTGGCTCAA CAACCTGGCG GAGAAAGCCA AACGCTGTAA CCGACGCCTT AAGTCCCAGA 
     2701 TCTTGCTTAA GAAATACCTC ATGAAGAGGC GCTGGAAGAA AAACTTCATT GCTGTCAGCG 
     2761 CTGCCAACCG CTTCAAGAAG ATCAGCAGCT CGGGGGCACT GATGGCTCTG GGGGTCACGC 
     2821 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     2881 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     2941 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     3001 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     3061 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     3121 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     3181 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     3241 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     3301 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     3361 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     3421 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     3481 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     3541 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     3601 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     3661 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     3721 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     3781 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     3841 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     3901 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     3961 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     4021 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     4081 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     4141 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     4201 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     4261 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     4321 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     4381 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     4441 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     4501 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     4561 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     4621 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     4681 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     4741 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     4801 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     4861 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     4921 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     4981 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     5041 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     5101 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     5161 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     5221 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     5281 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     5341 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     5401 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     5461 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     5521 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     5581 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     5641 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     5701 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     5761 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     5821 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     5881 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     5941 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     6001 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6061 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     6121 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     6181 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     6241 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     6301 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     6361 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     6421 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     6481 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     6541 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     6601 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     6661 GCGAATTTT
//