LOCUS       RC213556L2                8913 bp    DNA     circular  SYN 
DEFINITION  TIAM2 (Myc-DDK-tagged)-Human T-cell lymphoma invasion and metastasis 2 (TIAM2), transcript variant 2
ACCESSION   NM_001010927
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7204..7384 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8112..8771 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7432..8051 
                     /label="ColE1"  
     misc_feature    6079..6099 
                     /label="LR50"  
     misc_feature    5219..5944 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    6088..6679 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5198..5204 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5198 
                     /label="TIAM2"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGAAGGACCG CGGGAGAATC AGGATCCTCC 
     3361 TCCGAGGCCT CTGGCCCGCC ACCTGTCTGA TGCAGACCGC CTCCGCAAAG TCATCCAGGA 
     3421 GCTTGTGGAC ACAGAGAAGT CCTACGTGAA GGATTTGAGC TGCCTCTTTG AATTATACTT 
     3481 GGAGCCACTT CAGAATGAGA CCTTTCTTAC CCAAGATGAG ATGGAGTCAC TTTTTGGAAG 
     3541 TTTGCCAGAG ATGCTTGAGT TTCAGAAGGT GTTTCTGGAG ACCCTGGAGG ATGGGATTTC 
     3601 AGCATCATCT GACTTTAACA CCCTAGAAAC CCCCTCACAG TTTAGAAAAT TACTGTTTTC 
     3661 CCTTGGAGGC TCTTTCCTTT ATTACGCGGA CCACTTTAAA CTGTACAGTG GATTCTGTGC 
     3721 TAACCATATC AAAGTACAGA AGGTTCTGGA GCGAGCTAAA ACTGACAAAG CCTTCAAGGC 
     3781 TTTTCTGGAC GCCCGGAACC CCACCAAGCA GCATTCCTCC ACGCTGGAGT CCTACCTCAT 
     3841 CAAGCCGGTT CAGAGAGTGC TCAAGTACCC GCTGCTGCTC AAGGAGCTGG TGTCCCTGAC 
     3901 GGACCAGGAG AGCGAGGAGC ACTACCACCT GACGGAAGCA CTAAAGGCAA TGGAGAAAGT 
     3961 AGCGAGCCAC ATCAATGAGA TGCAGAAGAT CTATGAGGAT TATGGGACCG TGTTTGACCA 
     4021 GCTAGTAGCT GAGCAGAGCG GAACAGAGAA GGAGGTAACA GAACTTTCGA TGGGAGAGCT 
     4081 TCTGATGCAC TCTACGGTTT CCTGGTTGAA TCCATTTCTG TCTCTAGGAA AAGCTAGAAA 
     4141 GGACCTTGAG CTCACAGTAT TTGTTTTTAA GAGAGCCGTC ATACTGGTTT ATAAAGAAAA 
     4201 CTGCAAACTG AAAAAGAAAT TGCCCTCGAA TTCCCGGCCT GCACACAACT CTACTGACTT 
     4261 GGACCCATTT AAATTCCGCT GGTTGATCCC CATCTCCGCG CTTCAAGTCA GACTGGGGAA 
     4321 TCCAGCAGGG ACAGAAAATA ATTCCATATG GGAACTGATC CATACGAAGT CAGAAATAGA 
     4381 AGGACGGCCA GAAACCATCT TTCAGTTGTG TTGCAGTGAC AGTGAAAGCA AAACCAACAT 
     4441 TGTTAAGGTG ATTCGTTCTA TTCTGAGGGA GAACTTCAGG CGTCACATAA AGTGTGAATT 
     4501 ACCACTGGAG AAAACGTGTA AGGATCGCCT GGTACCTCTT AAGAACCGAG TTCCTGTTTC 
     4561 GGCCAAATTA GCTTCATCCA GGTCTTTAAA AGTCCTGAAG AATTCCTCCA GCAACGAGTG 
     4621 GACCGGTGAG ACTGGCAAGG GAACCTTGCT GGACTCTGAC GAGGGCAGCT TGAGCAGCGG 
     4681 CACCCAGAGC AGCGGCTGCC CCACGGCTGA GGGCAGGCAG GACTCCAAGA GCACTTCTCC 
     4741 CGGGAAATAC CCACACCCCG GCTTGGCAGA TTTTGCCGAC AATCTCATCA AAGAGAGTGA 
     4801 CATCCTGAGC GATGAAGATG ATGACCACCG TCAGACTGTG AAGCAGGGCA GCCCTACTAA 
     4861 AGACATCGAA ATTCAGTTCC AGAGACTGAG GATTTCCGAG GACCCAGACG TTCACCCCGA 
     4921 GGCTGAGCAG CAGCCTGGCC CGGAGTCGGG TGAGGGTCAG AAAGGAGGAG AGCAGCCCAA 
     4981 ACTGGTCCGG GGGCACTTCT GCCCCATTAA ACGAAAAGCC AACAGCACCA AGAGGGACAG 
     5041 AGGAACTTTG CTCAAGGCGC AGATCCGTCA CCAGTCCCTT GACAGTCAGT CTGAAAATGC 
     5101 CACCATCGAC CTAAATTCTG TTCTAGAGCG AGAATTCAGT GTCCAGAGTT TAACATCTGT 
     5161 TGTCAGTGAG GAGTGTTTTT ATGAAACAGA GAGCCACGGA AAATCAACGC GTACGCGGCC 
     5221 GCTCGAGATG AGCGGGGGCG AGGAGCTGTT CGCCGGCATC GTGCCCGTGC TGATCGAGCT 
     5281 GGACGGCGAC GTGCACGGCC ACAAGTTCAG CGTGCGCGGC GAGGGCGAGG GCGACGCCGA 
     5341 CTACGGCAAG CTGGAGATCA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC 
     5401 CACCCTGGTG ACCACCCTCT GCTACGGCAT CCAGTGCTTC GCCCGCTACC CCGAGCACAT 
     5461 GAAGATGAAC GACTTCTTCA AGAGCGCCAT GCCCGAGGGC TACATCCAGG AGCGCACCAT 
     5521 CCAGTTCCAG GACGACGGCA AGTACAAGAC CCGCGGCGAG GTGAAGTTCG AGGGCGACAC 
     5581 CCTGGTGAAC CGCATCGAGC TGAAGGGCAA GGACTTCAAG GAGGACGGCA ACATCCTGGG 
     5641 CCACAAGCTG GAGTACAGCT TCAACAGCCA CAACGTGTAC ATCCGCCCCG ACAAGGCCAA 
     5701 CAACGGCCTG GAGGCTAACT TCAAGACCCG CCACAACATC GAGGGCGGCG GCGTGCAGCT 
     5761 GGCCGACCAC TACCAGACCA ACGTGCCCCT GGGCGACGGC CCCGTGCTGA TCCCCATCAA 
     5821 CCACTACCTG AGCACTCAGA CCAAGATCAG CAAGGACCGC AACGAGGCCC GCGACCACAT 
     5881 GGTGCTCCTG GAGTCCTTCA GCGCCTGCTG CCACACCCAC GGCATGGACG AGCTGTACAG 
     5941 GTCCGGACTC AGATAAGTTT AAACGGCCGG CCGCGGTCTG TACAAGTAGG ATTCGTCGAG 
     6001 GGACCTAATA ACTTCGTATA GCATACATTA TACGAAGTTA TACATGTTTA AGGGTTCCGG 
     6061 TTCCACTAGG TACAATTCGA TATCAAGCTT ATCGATAATC AACCTCTGGA TTACAAAATT 
     6121 TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT 
     6181 GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG 
     6241 TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC 
     6301 GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT 
     6361 CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC 
     6421 GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG 
     6481 TTGTCGGGGA AATCATCGTC CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG 
     6541 CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC 
     6601 GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG 
     6661 ATCTCCCTTT GGGCCGCCTC CCCGCATCGA TACCGTCGAC CTCGATCGAG ACCTAGAAAA 
     6721 ACATGGAGCA ATCACAAGTA GCAATACAGC AGCTACCAAT GCTGATTGTG CCTGGCTAGA 
     6781 AGCACAAGAG GAGGAGGAGG TGGGTTTTCC AGTCACACCT CAGGTACCTT TAAGACCAAT 
     6841 GACTTACAAG GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA GAAAAGGGGG GACTGGAAGG 
     6901 GCTAATTCAC TCCCAACGAA GACAAGATAT CCTTGATCTG TGGATCTACC ACACACAAGG 
     6961 CTACTTCCCT GATTGGCAGA ACTACACACC AGGGCCAGGG ATCAGATATC CACTGACCTT 
     7021 TGGATGGTGC TACAAGCTAG TACCAGTTGA GCAAGAGAAG GTAGAAGAAG CCAATGAAGG 
     7081 AGAGAACACC CGCTTGTTAC ACCCTGTGAG CCTGCATGGG ATGGATGACC CGGAGAGAGA 
     7141 AGTATTAGAG TGGAGGTTTG ACAGCCGCCT AGCATTTCAT CACATGGCCC GAGAGCTGCA 
     7201 TCCGGACTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA 
     7261 ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG 
     7321 TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG 
     7381 GAAAATCTCT AGCAGCATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     7441 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     7501 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     7561 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     7621 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     7681 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     7741 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     7801 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     7861 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     7921 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     7981 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     8041 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     8101 TTAAGGGATT TTGGTCATGA TTACGCCCCG CCCTGCCACT CATCGCAGTA CTGTTGTAAT 
     8161 TCATTAAGCA TTCTGCCGAC ATGGAAGCCA TCACAAACGG CATGATGAAC CTGAATCGCC 
     8221 AGCGGCATCA GCACCTTGTC GCCTTGCGTA TAATATTTGC CCATGGTGAA AACGGGGGCG 
     8281 AAGAAGTTGT CCATATTGGC CACGTTTAAA TCAAAACTGG TGAAACTCAC CCAGGGATTG 
     8341 GCTGAGACGA AAAACATATT CTCAATAAAC CCTTTAGGGA AATAGGCCAG GTTTTCACCG 
     8401 TAACACGCCA CATCTTGCGA ATATATGTGT AGAAACTGCC GGAAATCGTC GTGGTATTCA 
     8461 CTCCAGAGCG ATGAAAACGT TTCAGTTTGC TCATGGAAAA CGGTGTAACA AGGGTGAACA 
     8521 CTATCCCATA TCACCAGCTC ACCGTCTTTC ATTGCCATAC GGAACTCCGG ATGAGCATTC 
     8581 ATCAGGCGGG CAAGAATGTG AATAAAGGCC GGATAAAACT TGTGCTTATT TTTCTTTACG 
     8641 GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA ACGGTCTGGT TATAGGTACA TTGAGCAACT 
     8701 GACTGAAATG CCTCAAAATG TTCTTTACGA TGCCATTGGG ATATATCAAC GGTGGTATAT 
     8761 CCAGTGATTT TTTTCTCCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8821 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8881 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GAC
//