LOCUS       RC213533                7560 bp    DNA     circular  SYN 
DEFINITION  MVP (Myc-DDK-tagged)-Human major vault protein (MVP), transcript variant 1
ACCESSION   NM_017458
VERSION     NM_017458.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3717..3808 
                     /label="Myc-DDK"  
     misc_feature    3838..4423 
                     /label="PolyA signal"  
     misc_feature    3895..3914 
                     /label="Restriction Enzyme"  
     misc_feature    4699..5371 
                     /label="ColE1"  
     misc_feature    5801..6595 
                     /label="Kan/Neo"  
     misc_feature    6630..6932 
                     /label="SV40 ori"  
     misc_feature    7018..7495 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3699..3705 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3699 
                     /label="MVP"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAACTGAA GAGTTCATCA TCCGCATCCC CCCATACCAC TATATCCATG 
     1081 TGCTGGACCA GAACAGCAAC GTGTCCCGTG TGGAGGTCGG GCCAAAGACC TACATCCGGC 
     1141 AGGACAATGA GAGGGTACTG TTTGCCCCCA TGCGCATGGT GACCGTCCCC CCACGTCACT 
     1201 ACTGCACAGT GGCCAACCCT GTGTCTCGGG ATGCCCAGGG CTTGGTGCTG TTTGATGTCA 
     1261 CAGGGCAAGT TCGGCTTCGC CACGCTGACC TCGAGATCCG GCTGGCCCAG GACCCCTTCC 
     1321 CCCTGTACCC AGGGGAGGTG CTGGAAAAGG ACATCACACC CCTGCAGGTG GTTCTGCCCA 
     1381 ACACTGCCCT CCATCTAAAG GCGCTGCTTG ATTTTGAGGA TAAAGATGGA GACAAGGTGG 
     1441 TGGCAGGAGA TGAGTGGCTT TTCGAGGGAC CTGGCACGTA CATCCCCCGG AAGGAAGTGG 
     1501 AGGTCGTGGA GATCATTCAG GCCACCATCA TCAGGCAGAA CCAGGCTCTG CGGCTCAGGG 
     1561 CCCGCAAGGA GTGCTGGGAC CGGGACGGCA AGGAGAGGGT GACAGGGGAA GAATGGCTGG 
     1621 TCACCACAGT AGGGGCGTAC CTCCCAGCGG TGTTTGAGGA GGTTCTGGAT TTGGTGGACG 
     1681 CCGTCATCCT TACGGAAAAG ACAGCCCTGC ACCTCCGGGC TCGGCGGAAC TTCCGGGACT 
     1741 TCAGGGGAGT GTCCCGCCGC ACTGGGGAGG AGTGGCTGGT AACAGTGCAG GACACAGAGG 
     1801 CCCACGTGCC AGATGTCCAC GAGGAGGTGC TGGGGGTTGT GCCCATCACC ACCCTGGGCC 
     1861 CCCACAACTA CTGCGTGATT CTCGACCCTG TCGGACCGGA TGGCAAGAAT CAGCTGGGGC 
     1921 AGAAGCGCGT GGTCAAGGGA GAGAAGTCTT TTTTCCTCCA GCCAGGAGAG CAGCTGGAAC 
     1981 AAGGCATCCA GGATGTGTAT GTGCTGTCGG AGCAGCAGGG GCTGCTGCTG AGGGCCCTGC 
     2041 AGCCCCTGGA GGAGGGGGAG GATGAGGAGA AGGTCTCACA CCAGGCTGGG GACCACTGGC 
     2101 TCATCCGCGG ACCCCTGGAG TATGTGCCAT CTGCCAAAGT GGAGGTGGTG GAGGAGCGCC 
     2161 AGGCCATCCC TCTAGACGAG AACGAGGGCA TCTATGTGCA GGATGTCAAG ACCGGAAAGG 
     2221 TGCGCGCTGT GATTGGAAGC ACCTACATGC TGACCCAGGA CGAAGTCCTG TGGGAGAAAG 
     2281 AGCTGCCTCC CGGGGTGGAG GAGCTGCTGA ACAAGGGGCA GGACCCTCTG GCAGACAGGG 
     2341 GTGAGAAGGA CACAGCTAAG AGCCTCCAGC CCTTGGCGCC CCGGAACAAG ACCCGTGTGG 
     2401 TCAGCTACCG CGTGCCCCAC AACGCTGCGG TGCAGGTGTA CGACTACCGA GAGAAGCGAG 
     2461 CCCGCGTGGT CTTCGGGCCT GAGCTGGTGT CGCTGGGTCC TGAGGAGCAG TTCACAGTGT 
     2521 TGTCCCTCTC AGCTGGGCGG CCCAAGCGTC CCCATGCCCG CCGTGCGCTC TGCCTGCTGC 
     2581 TGGGGCCTGA CTTCTTCACA GACGTCATCA CCATCGAAAC GGCGGATCAT GCCAGGCTGC 
     2641 AACTGCAGCT GGCCTACAAC TGGCACTTTG AGGTGAATGA CCGGAAGGAC CCCCAAGAGA 
     2701 CGGCCAAGCT CTTTTCAGTG CCAGACTTTG TAGGTGATGC CTGCAAAGCC ATCGCATCCC 
     2761 GGGTGCGGGG GGCCGTGGCC TCTGTCACTT TCGATGACTT CCATAAGAAC TCAGCCCGCA 
     2821 TCATTCGCAC TGCTGTCTTT GGCTTTGAGA CCTCGGAAGC GAAGGGCCCC GATGGCATGG 
     2881 CCCTGCCCAG GCCCCGGGAC CAGGCTGTCT TCCCCCAAAA CGGGCTGGTG GTCAGCAGTG 
     2941 TGGACGTGCA GTCAGTGGAG CCTGTGGATC AGAGGACCCG GGACGCCCTG CAACGCAGCG 
     3001 TCCAGCTGGC CATCGAGATC ACCACCAACT CCCAGGAAGC GGCGGCCAAG CATGAGGCTC 
     3061 AGAGACTGGA GCAGGAAGCC CGCGGCCGGC TTGAGCGGCA GAAGATCCTG GACCAGTCAG 
     3121 AAGCCGAGAA AGCTCGCAAG GAACTTTTGG AGCTGGAGGC TCTGAGCATG GCCGTGGAGA 
     3181 GCACCGGGAC TGCCAAGGCG GAGGCCGAGT CCCGTGCGGA GGCAGCCCGG ATTGAGGGAG 
     3241 AAGGGTCCGT GCTGCAGGCC AAGCTAAAAG CACAGGCCTT GGCCATTGAA ACGGAGGCTG 
     3301 AGCTCCAGAG GGTCCAGAAG GTCCGAGAGC TGGAACTGGT CTATGCCCGG GCCCAGCTGG 
     3361 AGCTGGAGGT GAGCAAGGCT CAGCAGCTGG CTGAGGTGGA GGTGAAGAAG TTCAAGCAGA 
     3421 TGACAGAGGC CATAGGCCCC AGCACCATCA GGGACCTTGC TGTGGCTGGG CCTGAGATGC 
     3481 AGGTAAAACT GCTCCAGTCC CTGGGCCTGA AATCAACCCT CATCACCGAT GGCTCCACTC 
     3541 CCATCAACCT CTTCAACACA GCCTTTGGGC TGCTGGGGAT GGGGCCCGAG GGTCAGCCCC 
     3601 TGGGCAGAAG GGTGGCCAGT GGGCCCAGCC CTGGGGAGGG GATATCCCCC CAGTCTGCTC 
     3661 AGGCCCCTCA AGCTCCTGGA GACAACCACG TGGTGCCTGT ACTGCGCACG CGTACGCGGC 
     3721 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3781 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3841 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     3901 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     3961 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4021 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4081 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4141 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4201 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4261 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4321 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4381 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4441 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4501 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4561 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4621 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4681 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4741 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4801 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     4861 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     4921 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     4981 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5041 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5101 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5161 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5221 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5281 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5341 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5401 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5461 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5521 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5581 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5641 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5701 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5761 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     5821 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     5881 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     5941 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6001 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6061 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6121 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6181 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6241 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6301 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6361 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6421 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6481 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6541 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6601 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6661 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6721 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6781 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     6841 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     6901 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     6961 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7021 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7081 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7141 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7201 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7261 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7321 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7381 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7441 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7501 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//