LOCUS       RC213517                8667 bp    DNA     circular  SYN 
DEFINITION  DIAPH1 (Myc-DDK-tagged)-Human diaphanous homolog 1 (Drosophila) (DIAPH1), transcript variant 2
ACCESSION   NM_001079812
VERSION     NM_001079812.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4824..4915 
                     /label="Myc-DDK"  
     misc_feature    4945..5530 
                     /label="PolyA signal"  
     misc_feature    5002..5021 
                     /label="Restriction Enzyme"  
     misc_feature    5806..6478 
                     /label="ColE1"  
     misc_feature    6908..7702 
                     /label="Kan/Neo"  
     misc_feature    7737..8039 
                     /label="SV40 ori"  
     misc_feature    8125..8602 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4806..4812 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4806 
                     /label="DIAPH1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCGCCC GGCGGGAGCC TGGGGCCCGG CCGCGGGACC CGGGACAAGA 
     1081 AGAAGGGCCG GAGCCCAGAT GAGCTGCCCT CGGCGGGCGG CGACGGCGGC AAATCTAAGA 
     1141 AATTTCTGGA GAGATTTACC AGCATGAGAA TTAAGAAGGA GAAGGAAAAG CCCAATTCTG 
     1201 CTCATAGAAA TTCTTCTGCA TCATATGGGG ATGATCCCAC AGCACAGTCA TTGCAAGATG 
     1261 TTTCAGATGA ACAAGTGCTG GTTCTCTTTG AACAGATGCT GCTGGATATG AACCTGAATG 
     1321 AGGAGAAACA GCAACCTTTG AGGGAGAAGG ACATCATCAT CAAGAGGGAG ATGGTGTCCC 
     1381 AATACTTGTA CACCTCCAAG GCTGGCATGA GCCAGAAGGA GAGCTCTAAG TCTGCCATGA 
     1441 TGTATATTCA GGAGTTGAGG TCAGGCTTGC GGGATATGCC TCTGCTCAGC TGCCTGGAGT 
     1501 CCCTTCGTGT GTCTCTCAAC AACAACCCTG TCAGTTGGGT GCAAACATTT GGTGCTGAAG 
     1561 GCTTGGCCTC CTTATTGGAC ATTCTTAAAC GACTTCATGA TGAGAAAGAA GAGACTGCTG 
     1621 GGAGTTACGA TAGCCGGAAC AAGCATGAGA TCATTCGCTG CTTGAAAGCT TTTATGAACA 
     1681 ACAAGTTTGG AATCAAGACC ATGTTGGAGA CAGAAGAAGG AATCCTACTG CTGGTCAGAG 
     1741 CCATGGATCC TGCTGTTCCC AACATGATGA TTGATGCAGC TAAGCTGCTT TCTGCTCTTT 
     1801 GTATTCTACC GCAGCCAGAG GACATGAATG AAAGGGTTTT GGAGGCAATG ACAGAAAGAG 
     1861 CTGAGATGGA TGAAGTGGAA CGTTTCCAGC CGCTGCTGGA TGGATTAAAA AGTGGAACCA 
     1921 CTATTGCACT GAAGGTTGGA TGCCTACAGC TGATCAATGC TCTCATCACA CCAGCGGAGG 
     1981 AACTTGACTT CCGAGTTCAC ATCAGAAGTG AACTGATGCG TTTGGGGCTA CATCAGGTGT 
     2041 TGCAGGACCT TCGAGAGATT GAAAATGAAG ATATGAGAGT GCAACTAAAT GTGTTTGATG 
     2101 AACAAGGGGA AGAGGATTCC TATGACCTGA AGGGACGGCT GGATGACATT CGCATGGAGA 
     2161 TGGATGACTT TAATGAAGTC TTTCAGATTC TCTTAAACAC AGTGAAGGAT TCAAAGGCAG 
     2221 AGCCACACTT CCTTTCCATC CTGCAGCACT TACTCTTGGT CCGAAATGAC TATGAGGCCA 
     2281 GACCTCAGTA CTATAAGTTG ATTGAAGAAT GTATTTCCCA GATAGTTCTG CACAAGAACG 
     2341 GGGCTGATCC TGACTTCAAG TGCCGGCACC TCCAGATTGA GATTGAGGGA TTAATTGATC 
     2401 AAATGATTGA TAAGACAAAG GTGGAGAAAT CTGAAGCCAA AGCTGCAGAG CTGGAAAAGA 
     2461 AGTTGGACTC AGAGTTAACA GCCCGACATG AGCTACAGGT GGAAATGAAA AAGATGGAAA 
     2521 GTGACTTTGA GCAGAAGCTT CAAGATCTTC AGGGAGAAAA AGATGCACTG CATTCTGAAA 
     2581 AGCAGCAAAT TGCCACAGAG AAACAGGACC TGGAAGCAGA GGTGTCCCAG CTCACAGGAG 
     2641 AGGTTGCCAA GCTGACAAAG GAACTGGAAG ATGCCAAGAA AGAAATGGCT TCCCTCTCTG 
     2701 CGGCAGCTAT TACTGTACCT CCTTCTGTTC CTAGTCGTGC TCCTGTTCCC CCTGCCCCTC 
     2761 CTTTACCTGG TGACTCTGGC ACTATTATTC CACCACCACC TGCTCCTGGG GATAGTACCA 
     2821 CTCCTCCTCC TCCTCCTCCT CCTCCTCCTC CACCTCCTTT GCCTGGGGGT GTTTGCATCT 
     2881 CCTCACCCCC TTCTTTACCT GGAGGTACTG CTATCTCTCC ACCCCCTCCT TTGTCTGGGG 
     2941 ATGCTACCAT CCCTCCACCC CCTCCTTTGC CTGAGGGTGT TGGCATCCCT TCACCCTCTT 
     3001 CTTTGCCTGG AGGTACTGCC ATCCCCCCAC CTCCTCCTTT GCCTGGGAGT GCTAGAATCC 
     3061 CCCCACCACC ACCTCCTTTG CCTGGGAGTG CTGGAATTCC CCCCCCACCT CCTCCCTTGC 
     3121 CTGGAGAAGC AGGAATGCCA CCTCCTCCTC CCCCTCTTCC TGGTGGTCCT GGAATCCCTC 
     3181 CACCTCCTCC ATTTCCCGGA GGCCCTGGCA TTCCTCCACC TCCACCCGGA ATGGGTATGC 
     3241 CTCCACCTCC CCCATTTGGA TTTGGAGTTC CTGCAGCCCC AGTTCTGCCA TTTGGATTAA 
     3301 CCCCCAAAAA GCTTTATAAG CCAGAGGTGC AGCTCCGGAG GCCAAACTGG TCCAAGCTTG 
     3361 TGGCTGAGGA CCTCTCCCAG GACTGCTTCT GGACAAAGGT GAAGGAGGAC CGCTTTGAGA 
     3421 ACAATGAACT TTTCGCCAAA CTTACCCTTA CCTTCTCTGC CCAGACCAAG ACTTCCAAAG 
     3481 CCAAGAAGGA TCAAGAAGGT GGAGAAGAAA AGAAATCTGT GCAAAAGAAA AAAGTAAAAG 
     3541 AGTTAAAGGT GTTGGATTCA AAGACAGCCC AGAATCTCTC AATCTTTTTG GGTTCCTTCC 
     3601 GCATGCCCTA TCAAGAGATT AAGAATGTCA TCCTGGAGGT GAATGAGGCT GTTCTGACTG 
     3661 AGTCTATGAT CCAGAACCTC ATTAAGCAAA TGCCAGAGCC AGAGCAGTTA AAAATGCTTT 
     3721 CTGAACTGAA GGATGAATAT GATGACCTGG CTGAGTCAGA GCAGTTTGGC GTGGTGATGG 
     3781 GCACTGTGCC CCGACTGCGG CCTCGCCTCA ATGCCATTCT CTTCAAGCTA CAATTCAGCG 
     3841 AGCAAGTGGA GAATATCAAG CCAGAGATTG TGTCTGTCAC TGCTGCATGT GAGGAGTTAC 
     3901 GTAAGAGTGA GAGCTTTTCC AATCTCCTAG AGATTACCTT GCTTGTTGGA AATTACATGA 
     3961 ATGCTGGCTC CAGAAATGCT GGTGCTTTTG GCTTCAATAT CAGCTTCCTC TGTAAGCTTC 
     4021 GAGACACCAA GTCCACAGAT CAGAAGATGA CGTTGTTACA CTTCTTGGCT GAGTTGTGTG 
     4081 AGAATGACTA TCCCGATGTC CTCAAGTTTC CAGACGAGCT TGCCCATGTG GAGAAAGCCA 
     4141 GCCGAGTTTC TGCTGAAAAC TTGCAAAAGA ACCTAGATCA GATGAAGAAA CAAATTTCTG 
     4201 ATGTGGAACG TGATGTTCAG AATTTCCCAG CTGCCACAGA TGAAAAAGAC AAGTTTGTTG 
     4261 AAAAAATGAC CAGCTTTGTG AAGGATGCAC AGGAACAGTA TAACAAGCTG CGGATGATGC 
     4321 ATTCTAACAT GGAGACCCTC TATAAGGAGC TGGGCGAGTA CTTCCTCTTT GACCCCAAGA 
     4381 AGTTGTCTGT TGAAGAATTT TTCATGGATC TTCACAATTT TCGGAATATG TTTTTGCAAG 
     4441 CAGTCAAGGA GAACCAGAAG CGGCGGGAGA CAGAAGAAAA GATGAGGCGA GCAAAACTAG 
     4501 CCAAGGAGAA GGCAGAGAAG GAGCGGCTAG AGAAGCAGCA GAAGAGAGAG CAACTCATAG 
     4561 ACATGAATGC AGAGGGCGAT GAGACAGGTG TGATGGACAG TCTTCTAGAA GCCCTGCAGT 
     4621 CAGGGGCAGC ATTCCGACGG AAGAGAGGGC CCCGTCAAGC CAACAGGAAG GCCGGGTGTG 
     4681 CAGTCACATC TCTGCTAGCT TCGGAGCTGA CCAAGGATGA TGCCATGGCT GCTGTTCCTG 
     4741 CCAAGGTGTC CAAGAACAGT GAGACATTCC CCACAATCCT TGAGGAAGCC AAGGAGTTGG 
     4801 TTGGCCGTGC AAGCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4861 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4921 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4981 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5041 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5101 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5161 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5221 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5281 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5341 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5401 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5461 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5521 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5581 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5641 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5701 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5761 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5821 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5881 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5941 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6001 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6061 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6121 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6181 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6241 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6301 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6361 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6421 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6481 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6541 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6601 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6661 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6721 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6781 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6841 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6901 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6961 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7021 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7081 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7141 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7201 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7261 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7321 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7381 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7441 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7501 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7561 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7621 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7681 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7741 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7801 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7861 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7921 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7981 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8041 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8101 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8161 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8221 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8281 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8341 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8401 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8461 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8521 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8581 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8641 ATTTAACAAA AATTTAACGC GAATTTT
//