LOCUS       RC213497                6516 bp    DNA     circular  SYN 
DEFINITION  AIRE (Myc-DDK-tagged)-Human autoimmune regulator (AIRE), transcript variant AIRE-1
ACCESSION   NM_000383
VERSION     NM_000383.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2673..2764 
                     /label="Myc-DDK"  
     misc_feature    2794..3379 
                     /label="PolyA signal"  
     misc_feature    2851..2870 
                     /label="Restriction Enzyme"  
     misc_feature    3655..4327 
                     /label="ColE1"  
     misc_feature    4757..5551 
                     /label="Kan/Neo"  
     misc_feature    5586..5888 
                     /label="SV40 ori"  
     misc_feature    5974..6451 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2655..2661 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2655 
                     /label="AIRE"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGACGGAC GCGGCGCTAC GCCGGCTTCT GAGGCTGCAC CGCACGGAGA 
     1081 TCGCGGTGGC CGTGGACAGC GCCTTCCCAC TGCTGCACGC GCTGGCTGAC CACGACGTGG 
     1141 TCCCCGAGGA CAAGTTTCAG GAGACGCTTC ATCTGAAGGA AAAGGAGGGC TGCCCCCAGG 
     1201 CCTTCCACGC CCTCCTGTCC TGGCTGCTGA CCCAGGACTC CACAGCCATC CTGGACTTCT 
     1261 GGAGGGTGCT GTTCAAGGAC TACAACCTGG AGCGCTATGG CCGGCTGCAG CCCATCCTGG 
     1321 ACAGCTTCCC CAAAGATGTG GACCTCAGCC AGCCCCGGAA GGGGAGGAAG CCCCCGGCCG 
     1381 TCCCCAAGGC TTTGGTACCG CCACCCAGAC TCCCCACCAA GAGGAAGGCC TCAGAAGAGG 
     1441 CTCGAGCTGC CGCGCCAGCA GCCCTGACTC CAAGGGGCAC CGCCAGCCCA GGCTCTCAAC 
     1501 TGAAGGCCAA GCCCCCCAAG AAGCCGGAGA GCAGCGCAGA GCAGCAGCGC CTTCCACTCG 
     1561 GGAACGGGAT TCAGACCATG TCAGCTTCAG TCCAGAGAGC TGTGGCCATG TCCTCCGGGG 
     1621 ACGTCCCGGG AGCCCGAGGG GCCGTGGAGG GGATCCTCAT CCAGCAGGTG TTTGAGTCAG 
     1681 GCGGCTCCAA GAAGTGCATC CAGGTTGGCG GGGAGTTCTA CACTCCCAGC AAGTTCGAAG 
     1741 ACTCCGGCAG TGGGAAGAAC AAGGCCCGCA GCAGCAGTGG CCCGAAGCCT CTGGTTCGAG 
     1801 CCAAGGGAGC CCAGGGCGCT GCCCCCGGTG GAGGTGAGGC TAGGCTGGGC CAGCAGGGCA 
     1861 GCGTTCCCGC CCCTCTGGCC CTCCCCAGTG ACCCCCAGCT CCACCAGAAG AATGAGGACG 
     1921 AGTGTGCCGT GTGTCGGGAC GGCGGGGAGC TCATCTGCTG TGACGGCTGC CCTCGGGCCT 
     1981 TCCACCTGGC CTGCCTGTCC CCTCCGCTCC GGGAGATCCC CAGTGGGACC TGGAGGTGCT 
     2041 CCAGCTGCCT GCAGGCAACA GTCCAGGAGG TGCAGCCCCG GGCAGAGGAG CCCCGGCCCC 
     2101 AGGAGCCACC CGTGGAGACC CCGCTCCCCC CGGGGCTTAG GTCGGCGGGA GAGGAGGTAA 
     2161 GAGGTCCACC TGGGGAACCC CTAGCCGGCA TGGACACGAC TCTTGTCTAC AAGCACCTGC 
     2221 CGGCTCCGCC TTCTGCAGCC CCGCTGCCAG GGCTGGACTC CTCGGCCCTG CACCCCCTAC 
     2281 TGTGTGTGGG TCCTGAGGGT CAGCAGAACC TGGCTCCTGG TGCGCGTTGC GGGGTGTGCG 
     2341 GAGATGGTAC GGACGTGCTG CGGTGTACTC ACTGCGCCGC TGCCTTCCAC TGGCGCTGCC 
     2401 ACTTCCCAGC CGGCACCTCC CGGCCCGGGA CGGGCCTGCG CTGCAGATCC TGCTCAGGAG 
     2461 ACGTGACCCC AGCCCCTGTG GAGGGGGTGC TGGCCCCCAG CCCCGCCCGC CTGGCCCCTG 
     2521 GGCCTGCCAA GGATGACACT GCCAGTCACG AGCCCGCTCT GCACAGGGAT GACCTGGAGT 
     2581 CCCTTCTGAG CGAGCACACC TTCGATGGCA TCCTGCAGTG GGCCATCCAG AGCATGGCCC 
     2641 GTCCGGCGGC CCCCTTCCCC TCCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     2701 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     2761 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     2821 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     2881 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     2941 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3001 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3061 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     3121 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     3181 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     3241 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     3301 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     3361 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     3421 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     3481 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     3541 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     3601 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     3661 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     3721 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     3781 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     3841 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     3901 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     3961 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4021 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4081 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     4141 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     4201 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     4261 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     4321 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     4381 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     4441 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     4501 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     4561 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     4621 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     4681 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     4741 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     4801 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     4861 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     4921 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     4981 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5041 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     5101 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     5161 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     5221 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     5281 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     5341 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     5401 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     5461 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     5521 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     5581 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     5641 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     5701 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     5761 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     5821 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     5881 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     5941 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6001 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6061 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     6121 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     6181 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     6241 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     6301 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     6361 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     6421 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     6481 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//