LOCUS       RC213487                7941 bp    DNA     circular  SYN 
DEFINITION  NEFH (Myc-DDK-tagged)-Human neurofilament, heavy polypeptide (NEFH)
ACCESSION   NM_021076
VERSION     NM_021076.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4098..4189 
                     /label="Myc-DDK"  
     misc_feature    4219..4804 
                     /label="PolyA signal"  
     misc_feature    4276..4295 
                     /label="Restriction Enzyme"  
     misc_feature    5080..5752 
                     /label="ColE1"  
     misc_feature    6182..6976 
                     /label="Kan/Neo"  
     misc_feature    7011..7313 
                     /label="SV40 ori"  
     misc_feature    7399..7876 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4080..4086 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4080 
                     /label="NEFH"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGAGCTTC GGCGGCGCGG ACGCGCTGCT GGGCGCCCCG TTCGCGCCGC 
     1081 TGCATGGCGG CGGCAGCCTC CACTACGCGC TAGCCCGAAA GGGTGGCGCA GGCGGGACGC 
     1141 GCTCCGCCGC TGGCTCCTCC AGCGGCTTCC ACTCGTGGAC ACGGACGTCC GTGAGCTCCG 
     1201 TGTCCGCCTC GCCCAGCCGC TTCCGTGGCG CAGGCGCCGC CTCAAGCACC GACTCGCTGG 
     1261 ACACGCTGAG CAACGGGCCG GAGGGCTGCA TGGTGGCGGT GGCCACCTCA CGCAGTGAGA 
     1321 AGGAGCAGCT GCAGGCGCTG AACGACCGCT TCGCCGGGTA CATCGACAAG GTGCGGCAGC 
     1381 TGGAGGCGCA CAACCGCAGC CTGGAGGGCG AGGCTGCGGC GCTGCGGCAG CAGCAGGCGG 
     1441 GCCGCTCCGC TATGGGCGAG CTGTACGAGC GCGAGGTCCG CGAGATGCGC GGCGCGGTGC 
     1501 TGCGCCTGGG CGCGGCGCGC GGTCAGCTAC GCCTGGAGCA GGAGCACCTG CTCGAGGACA 
     1561 TCGCGCACGT GCGCCAGCGC CTAGACGACG AGGCCCGGCA GCGAGAGGAG GCCGAGGCGG 
     1621 CGGCCCGCGC GCTGGCGCGC TTCGCGCAGG AGGCCGAGGC GGCGCGCGTG GACCTGCAGA 
     1681 AGAAGGCGCA GGCGCTGCAG GAGGAGTGCG GCTACCTGCG GCGCCAYCAC CAGGAAGAGG 
     1741 TGGGCGAGCT GCTCGGCCAG ATCCAGGGCT CCGGCGCCGC GCAGGCGCAG ATGCAGGCCG 
     1801 AGACGCGCGA CGCCCTGAAG TGCGACGTGA CGTCGGCGCT GCGCGAGATT CGCGCGCAGC 
     1861 TTGAAGGCCA CGCGGTGCAG AGCACGCTGC AGTCCGAGGA GTGGTTCCGA GTGAGGCTGG 
     1921 ACCGACTGTC GGAGGCAGCC AAGGTGAACA CAGACGCTAT GCGCTCAGCG CAGGAGGAGA 
     1981 TAACTGAGTA CCGGCGTCAG CTGCAGGCCA GGACCACAGA GCTGGAGGCA CTGAAAAGCA 
     2041 CCAAGGACTC ACTGGAGAGG CAGCGCTCTG AGCTGGAGGA CCGTCATCAG GCCGACATTG 
     2101 CCTCCTACCA GGAAGCCATT CAGCAGCTGG ACGCTGAGCT GAGGAACACC AAGTGGGAGA 
     2161 TGGCCGCCCA GCTGCGAGAA TACCAGGACC TGCTCAATGT CAAGATGGCT CTGGATATAG 
     2221 AGATAGCCGC TTACAGAAAA CTCCTGGAAG GTGAAGAGTG TCGGATTGGC TTTGGCCCAA 
     2281 TTCCTTTCTC GCTTCCAGAA GGACTCCCCA AAATTCCCTC TGTGTCCACT CACATAAAGG 
     2341 TGAAAAGCGA AGAGAAGATC AAAGTGGTGG AGAAGTCTGA GAAAGAAACT GTGATTGTGG 
     2401 AGGAACAGAC AGAGGAGACC CAAGTGACTG AAGAAGTGAC TGAAGAAGAG GAGAAAGAGG 
     2461 CCAAAGAGGA GGAGGGCAAG GAGGAAGAAG GGGGTGAAGA AGAGGAGGCA GAAGGGGGAG 
     2521 AAGAAGAAAC AAAGTCTCCC CCAGCAGAAG AGGCTGCATC CCCAGAGAAG GAAGCCAAGT 
     2581 CACCAGTAAA GGAAGAGGCA AAGTCACCGG CTGAGGCCAA GTCCCCAGAG AAGGAGGAAG 
     2641 CAAAATCCCC AGCCGAAGTC AAGTCCCCTG AGAAGGCCAA GTCTCCAGCA AAGGAAGAGG 
     2701 CAAAGTCACC GCCTGAGGCC AAGTCCCCAG AGAAGGAGGA AGCAAAATCT CCAGCTGAGG 
     2761 TCAAGTCCCC CGAGAAGGCC AAGTCCCCAG CAAAGGAAGA GGCAAAGTCA CCGGCTGAGG 
     2821 CCAAGTCTCC AGAGAAGGCC AAGTCCCCAG TGAAGGAAGA AGCAAAGTCA CTGGCTGAGG 
     2881 CCAAGTCCCC AGTGAAGGAA GAAGCAAAAT CTCCAGCTGA GGTCAAGTCC CCGGAAAAGG 
     2941 CCAAGTCTCC AACGAAGGAG GAAGCAAAGT CCCCTGAGAA GGCCAAGTCC CCAGAGAAGG 
     3001 AAGAGGCCAA GTCCCCTGAG AAGGCCAAGT CCCCAGTGAA GGCAGAAGCA AAGTCCCCTG 
     3061 AGAAGGCCAA GTCCCCAGTG AAGGCAGAAG CAAAGTCCCC TGAGAAGGCC AAGTCCCCAG 
     3121 TGAAGGAAGA AGCAAAGTCC CCTGAGAAGG CCAAGTCCCC AGTGAAGGAA GAAGCAAAGT 
     3181 CCCCTGAGAA GGCCAAGTCC CCAGTGAAGG AAGAAGCAAA GACCCCCGAG AAGGCCAAGT 
     3241 CCCCAGTGAA GGAAGAAGCT AAGTCCCCAG AGAAGGCCAA GTCCCCAGAG AAGGCCAAGA 
     3301 CTCTTGATGT GAAGTCTCCA GAAGCCAAGA CTCCAGCGAA GGAGGAAGCA AGGTCCCCTG 
     3361 CAGACAAATT CCCTGAAAAG GCCAAAAGCC CTGTCAAGGA GGAGGTCAAG TCCCCAGAGA 
     3421 AGGCGAAATC TCCCCTGAAG GAGGATGCCA AGGCCCCTGA GAAGGAGATC CCAAAAAAGG 
     3481 AAGAGGTGAA GTCCCCAGTG AAGGAGGAGG AGAAGCCCCA GGAGGTGAAA GTCAAAGAGC 
     3541 CCCCAAAGAA GGCAGAGGAA GAGAAAGCCC CTGCCACACC AAAAACAGAG GAGAAGAAGG 
     3601 ACAGCAAGAA AGAGGAGGCA CCCAAGAAGG AGGCTCCAAA GCCCAAGGTG GAGGAGAAGA 
     3661 AGGAACCTGC TGTCGAAAAG CCCAAAGAAT CCAAAGTTGA AGCCAAGAAG GAAGAGGCTG 
     3721 AAGATAAGAA AAAAGTCCCC ACCCCAGAGA AGGAGGCTCC TGCCAAGGTG GAGGTGAAGG 
     3781 AAGACGCTAA ACCCAAAGAA AAGACAGAGG TAGCCAAGAA GGAACCAGAT GATGCCAAGG 
     3841 CCAAGGAACC CAGCAAACCA GCAGAGAAGA AGGAGGCAGC ACCGGAGAAA AAAGACACCA 
     3901 AGGAGGAGAA GGCCAAGAAG CCTGAGGAGA AACCCAAGAC AGAGGCCAAA GCCAAGGAAG 
     3961 ATGACAAGAC CCTCTCAAAA GAGCCTAGCA AGCCTAAGGC AGAAAAGGCT GAAAAATCCT 
     4021 CCAGCACAGA CCAAAAAGAC AGCAAGCCTC CAGAGAAGGC CACAGAAGAC AAGGCCGCCA 
     4081 AGGGGAAGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4141 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4201 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4261 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4321 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4381 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4441 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4501 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4561 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4621 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4681 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4741 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4801 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4861 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4921 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4981 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5041 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5101 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5161 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5221 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5281 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5341 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5401 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5461 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5521 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5581 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5641 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5701 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5761 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5821 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5881 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5941 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6001 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6061 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6121 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6181 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6241 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6301 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6361 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6421 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6481 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6541 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6601 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6661 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6721 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6781 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6841 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6901 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6961 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7021 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7081 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7141 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7201 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7261 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7321 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7381 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7441 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7501 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7561 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7621 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7681 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7741 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7801 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7861 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7921 CAAAAATTTA ACGCGAATTT T
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