LOCUS       RC213422                8622 bp    DNA     circular  SYN 
DEFINITION  NID1 (Myc-DDK-tagged)-Human nidogen 1 (NID1)
ACCESSION   NM_002508
VERSION     NM_002508.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4779..4870 
                     /label="Myc-DDK"  
     misc_feature    4900..5485 
                     /label="PolyA signal"  
     misc_feature    4957..4976 
                     /label="Restriction Enzyme"  
     misc_feature    5761..6433 
                     /label="ColE1"  
     misc_feature    6863..7657 
                     /label="Kan/Neo"  
     misc_feature    7692..7994 
                     /label="SV40 ori"  
     misc_feature    8080..8557 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4761..4767 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4761 
                     /label="NID1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTGGCCTCG AGCAGCCGGA TCCGGGCTGC GTGGACGCGG GCGCTGCTGC 
     1081 TGCCGCTGCT GCTGGCGGGG CCTGTGGGCT GCCTGAGCCG CCAGGAGCTC TTTCCCTTCG 
     1141 GCCCCGGACA GGGGGACCTG GAGCTGGAGG ACGGGGATGA CTTCGTCTCT CCTGCCCTGG 
     1201 AGCTGAGTGG GGCGCTCCGC TTCTACGACA GATCCGACAT CGACGCAGTC TACGTCACCA 
     1261 CAAATGGCAT CATTGCTACG AGTGAACCCC CGGCCAAAGA ATCCCATCCC GGGCTCTTCC 
     1321 CACCAACATT CGGTGCAGTC GCCCCTTTCC TGGCGGACTT GGACACGACC GATGGCCTGG 
     1381 GGAAGGTTTA TTATCGAGAA GACTTATCCC CCTCCATCAC TCAGCGAGCA GCAGAGTGTG 
     1441 TCCACAGAGG GTTCCCGGAG ATCTCTTTCC AGCCTAGTAG CGCGGTGGTT GTCACTTGGG 
     1501 AATCCGTGGC CCCCTACCAA GGGCCCAGCA GGGACCCAGA CCAGAAAGGC AAGAGAAACA 
     1561 CGTTCCAGGC TGTTCTAGCC TCCTCTGATT CCAGCTCCTA TGCCATTTTC CTTTATCCTG 
     1621 AGGATGGTCT GCAGTTCCAT ACGACATTCT CAAAGAAGGA AAACAACCAA GTTCCTGCCG 
     1681 TGGTTGCATT CAGTCAAGGT TCAGTGGGAT TCTTATGGAA GAGCAACGGA GCTTATAACA 
     1741 TATTTGCTAA TGACAGGGAA TCAATTGAAA ATTTGGCCAA GAGTAGTAAC TCTGGGCAGC 
     1801 AGGGTGTCTG GGTGTTTGAG ATTGGGAGTC CAGCCACCAC CAATGGCGTG GTGCCTGCAG 
     1861 ACGTGATCCT CGGAACTGAA GATGGGGCAG AGTATGATGA TGAGGATGAA GATTATGACC 
     1921 TGGCGACCAC TCGTCTGGGC CTGGAGGATG TGGGCACCAC GCCCTTCTCC TACAAGGCTC 
     1981 TGAGAAGGGG AGGTGCTGAC ACATACAGTG TGCCCAGCGT CCTCTCCCCG CGCCGGGCAG 
     2041 CTACCGAAAG GCCCCTTGGA CCTCCCACAG AGAGAACCAG GTCTTTCCAG TTGGCAGTGG 
     2101 AGACTTTTCA CCAGCAGCAC CCTCAGGTCA TAGATGTGGA TGAAGTTGAG GAAACAGGAG 
     2161 TTGTTTTCAG CTATAACACG GATTCCCGCC AGACGTGTGC TAACAACAGA CACCAGTGCT 
     2221 CGGTGCACGC AGAGTGCAGG GACTACGCCA CGGGCTTCTG CTGCAGCTGT GTCGCTGGCT 
     2281 ATACGGGCAA TGGCAGGCAA TGTGTTGCAG AAGGTTCCCC CCAGCGAGTC AATGGCAAGG 
     2341 TGAAAGGAAG GATCTTTGTG GGGAGCAGCC AGGTCCCCAT TGTCTTTGAG AACACTGACC 
     2401 TCCACTCTTA CGTAGTAATG AACCACGGGC GCTCCTACAC AGCCATCAGC ACCATTCCCG 
     2461 AGACCGTTGG ATATTCTCTG CTTCCACTGG CCCCAGTTGG AGGCATCATT GGATGGATGT 
     2521 TTGCAGTGGA GCAGGACGGA TTCAAGAATG GGTTCAGCAT CACCGGGGGT GAGTTCACTC 
     2581 GCCAGGCTGA GGTGACCTTC GTGGGGCACC CGGGCAATCT GGTCATTAAG CAGCGGTTCA 
     2641 GCGGCATCGA TGAGCATGGG CACCTGACCA TCGACACGGA GCTGGAGGGC CGCGTGCCGC 
     2701 AGATTCCGTT CGGCTCCTCC GTGCACATTG AGCCCTACAC GGAGCTGTAC CACTACTCCA 
     2761 CCTCAGTGAT CACTTCCTCC TCCACCCGGG AGTACACGGT GACTGAGCCC GAGCGAGATG 
     2821 GGGCATCTCC TTCACGCATC TACACTTACC AGTGGCGCCA GACCATCACC TTCCAGGAAT 
     2881 GCGTCCACGA TGACTCCCGG CCAGCCCTGC CCAGCACCCA GCAGCTCTCG GTGGACAGCG 
     2941 TGTTCGTCCT GTACAACCAG GAGGAGAAGA TCTTGCGCTA TGCTCTCAGC AACTCCATTG 
     3001 GGCCTGTGAG GGAAGGCTCC CCTGATGCTC TTCAGAATCC CTGCTACATC GGCACTCATG 
     3061 GGTGTGACAC CAACGCGGCC TGTCGCCCTG GTCCCAGGAC ACAGTTCACC TGCGAGTGCT 
     3121 CCATCGGCTT CCGAGGAGAC GGGCGAACCT GCTATGATAT TGATGAATGT TCAGAACAAC 
     3181 CCTCAGTGTG TGGGAGCCAC ACAATCTGCA ATAATCACCC AGGAACCTTC CGCTGCGAGT 
     3241 GTGTGGAGGG CTACCAGTTT TCAGATGAGG GAACGTGTGT GGCTGTCGTG GACCAGCGCC 
     3301 CCATCAACTA CTGTGAAACT GGCCTTCATA ACTGCGACAT ACCCCAGCGG GCCCAGTGTA 
     3361 TCTACACAGG AGGCTCCTCC TACACCTGTT CCTGCTTGCC AGGCTTTTCT GGGGATGGCC 
     3421 AAGCCTGCCA AGATGTGGAT GAATGCCATC CAAGCCGATG TCACCCTGAC GCCTTCTGCT 
     3481 ACAACACTCC AGGCTCTTTC ACGTGCCAGT GCAAACCTGG TTATCAGGGA GACGGCTTCC 
     3541 GTTGCGTGCC CGGAGAGGTG GAGAAAACCC GGTGCCAGCA CGAGCGAGAA CACATTCTCG 
     3601 GGGCAGCGGG GGCGACAGAC CCACAGCGAC CCATTCCTCC GGGGCTGTTC GTTCCTGAGT 
     3661 GCGATGCGCA CGGGCACTAC GCGCCCACCC AGTGCCACGG CAGCACCGGC TACTGCTGGT 
     3721 GCGTGGATCG CGACGGCCGC GAGGTGGAGG GCACCAGGAC CAGGCCCGGG ATGACGCCCC 
     3781 CGTGTCTGAG TACAGTGGCT CCCCCGATTC ACCAAGGACC TGCGGTGCCT ACCGCCGTGA 
     3841 TCCCCTTGCC TCCTGGGACC CATTTACTCT TTGCCCAGAC TGGGAAGATT GAGCGCCTGC 
     3901 CCCTGGAGGG AAATACCATG AGGAAGACAG AAGCAAAGGC GTTCCTTCAT GTCCCGGCTA 
     3961 AAGTCATCAT TGGACTGGCC TTTGACTGCG TGGACAAGAT GGTTTACTGG ACGGACATCA 
     4021 CTGAGCCTTC CATTGGGAGA GCTAGTCTAC ATGGTGGAGA GCCAACCACC ATCATTAGAC 
     4081 AAGATCTTGG AAGTCCAGAA GGTATCGCTG TTGATCACCT TGGCCGCAAC ATCTTCTGGA 
     4141 CAGACTCTAA CCTGGATCGA ATAGAAGTGG CGAAGCTGGA CGGCACGCAG CGCCGGGTGC 
     4201 TCTTTGAGAC TGACTTGGTG AATCCCAGAG GCATTGTAAC GGATTCCGTG AGAGGGAACC 
     4261 TTTACTGGAC AGACTGGAAC AGAGATAACC CCAAGATTGA AACTTCCTAC ATGGACGGCA 
     4321 CGAACCGGAG GATCCTTGTG CAGGATGACC TGGGCTTGCC CAATGGACTG ACCTTCGATG 
     4381 CGTTCTCATC TCAGCTCTGC TGGGTGGATG CAGGCACCAA TCGGGCGGAA TGCCTGAACC 
     4441 CCAGTCAGCC CAGCAGACGC AAGGCTCTCG AAGGGCTCCA GTATCCTTTT GCTGTGACGA 
     4501 GCTACGGGAA GAATCTGTAT TTCACAGACT GGAAGATGAA TTCCGTGGTT GCTCTCGATC 
     4561 TTGCAATTTC CAAGGAGACG GATGCTTTCC AACCCCACAA GCAGACCCGG CTGTATGGCA 
     4621 TCACCACGGC CCTGTCTCAG TGTCCGCAAG GCCATAACTA CTGCTCAGTG AACAATGGCG 
     4681 GCTGCACCCA CCTATGCTTG GCCACCCCAG GGAGCAGGAC CTGCCGTTGC CCTGACAACA 
     4741 CCTTGGGAGT TGACTGTATC GAACAGAAAA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4801 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4861 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4921 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4981 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5041 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5101 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5161 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5221 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5281 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5341 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5401 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5461 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5521 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5581 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5641 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5701 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5761 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5821 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5881 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5941 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6001 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6061 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6121 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6181 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6241 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6301 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6361 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6421 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6481 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6541 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6601 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6661 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6721 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6781 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6841 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6901 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6961 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7021 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7081 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7141 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7201 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7261 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7321 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7381 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7441 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7501 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7561 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7621 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7681 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7741 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7801 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7861 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7921 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7981 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8041 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8101 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8161 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8221 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8281 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8341 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8401 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8461 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8521 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8581 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//