LOCUS       RC213399                7746 bp    DNA     circular  SYN 
DEFINITION  TJP3 (Myc-DDK-tagged)-Human tight junction protein 3 (zona occludens 3) (TJP3)
ACCESSION   NM_014428
VERSION     NM_014428.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3901..3992 
                     /label="Myc-DDK"  
     misc_feature    4022..4607 
                     /label="PolyA signal"  
     misc_feature    4079..4098 
                     /label="Restriction Enzyme"  
     misc_feature    4883..5555 
                     /label="ColE1"  
     misc_feature    5985..6779 
                     /label="Kan/Neo"  
     misc_feature    6814..7116 
                     /label="SV40 ori"  
     misc_feature    7202..7679 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3876..3883 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3876 
                     /label="TJP3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACCTGTGT GGCCTCATGC CCATCTTCCC CGCTCCCCTC GACCAGGTGG 
     1081 CTGACATGGA GGAGCTGACC ATCTGGGAAC AGCACACGGC CACACTGTCC AAGGACCCCC 
     1141 GCCGGGGCTT TGGCATTGCG ATCTCTGGAG GCCGAGACCG GCCCGGTGGA TCCATGGTTG 
     1201 TATCTGACGT GGTACCTGGA GGGCCGGCGG AGGGCAGGCT ACAGACAGGC GACCACATCG 
     1261 TCATGGTGAA CGGGGTTTCC ATGGAGAATG CCACCTCCGC GTTTGCCATT CAGATACTCA 
     1321 AGACCTGCAC CAAGATGGCC AACATCACAG TGAAACGTCC CCGGAGGATC CACCTGCCCG 
     1381 CCACCAAAGC CAGCCCCTCC AGCCCAGGGC GCCAGGACTC GGATGAAGAC GATGGGCCCC 
     1441 AGCGGGTGGA GGAGGTGGAC CAGGGCCGGG GCTATGACGG CGACTCATCC AGTGGCTCCG 
     1501 GCCGCTCCTG GGACGAGCGC TCCCGCCGGC CGAGGCCTGG TCGCCGGGGC CGGGCCGGCA 
     1561 GCCATGGGCG TAGGAGCCCA GGTGGTGGCT CTGAGGCCAA CGGGCTGGCC CTGGTGTCCG 
     1621 GCTTTAAGCG GCTGCCACGG CAGGACGTGC AGATGAAGCC TGTGAAGTCA GTGCTGGTGA 
     1681 AGAGGAGAGA CAGCGAAGAG TTTGGCGTCA AGCTGGGCAG TCAGATCTTC ATCAAGCACA 
     1741 TTACAGATTC GGGCCTGGCT GCCCGGCACC GTGGGCTGCA GGAAGGAGAT CTCATTCTAC 
     1801 AGATCAACGG GGTGTCTAGC CAGAACCTGT CACTGAACGA CACCCGGCGA CTGATTGAGA 
     1861 AGTCAGAAGG GAAGCTAAGC CTGCTGGTGC TGAGAGATCG TGGGCAGTTC CTGGTGAACA 
     1921 TTCCGCCTGC TGTCAGTGAC AGCGACAGCT CGCCATTGGA GGAAGGCGTG ACCATGGCTG 
     1981 ATGAGATGTC CTCTCCCCCT GCAGACATCT CGGACCTCGC CTCGGAGCTA TCGCAGGCAC 
     2041 CACCATCCCA CATCCCACCA CCACCCCGGC ATGCTCAGCG GAGCCCCGAG GCCAGCCAGA 
     2101 CCGACTCTCC CGTGGAGAGT CCCCGGCTTC GGCGGGAAAG TTCAGTAGAT TCCAGAACCA 
     2161 TCTCGGAACC AGATGAGCAA CGGTCAGAGT TGCCCAGGGA AAGCAGCTAT GACATCTACA 
     2221 GAGTGCCCAG CAGTCAGAGC ATGGAGGATC GTGGGTACAG CCCCGACACG CGTGTGGTCC 
     2281 GCTTCCTCAA GGGCAAGAGC ATCGGGCTGC GGCTGGCAGG GGGCAATGAC GTGGGCATCT 
     2341 TCGTGTCCGG GGTGCAGGCG GGCAGCCCGG CCGACGGGCA GGGCATCCAG GAGGGAGATC 
     2401 AGATTCTGCA GGTGAATGAC GTGCCATTCC AGAACCTGAC ACGGGAGGAG GCAGTGCAGT 
     2461 TCCTGCTGGG GCTGCCACCA GGCGAGGAGA TGGAGCTGGT GACGCAGAGG AAGCAGGACA 
     2521 TTTTCTGGAA AATGGTGCAG TCCCGCGTGG GTGACTCCTT CTACATCCGC ACTCACTTTG 
     2581 AGCTGGAGCC CAGTCCACCG TCTGGCCTGG GCTTCACCCG TGGCGACGTC TTCCACGTGC 
     2641 TGGACACGCT GCACCCCGGC CCCGGGCAGA GCCACGCACG AGGAGGCCAC TGGCTGGCGG 
     2701 TGCGCATGGG TCGTGACCTG CGGGAGCAAG AGCGGGGCAT CATTCCCAAC CAGAGCAGGG 
     2761 CGGAGCAGCT GGCCAGCCTG GAAGCTGCCC AGAGGGCCGT GGGAGTCGGG CCCGGCTCCT 
     2821 CCGCGGGCTC CAATGCTCGG GCCGAGTTCT GGCGGCTGCG GGGTCTTCGT CGAGGAGCCA 
     2881 AGAAGACCAC TCAGCGGAGC CGTGAGGACC TCTCAGCTCT GACCCGACAG GGCCGCTACC 
     2941 CGCCCTACGA ACGAGTGGTG TTGCGAGAAG CCAGTTTCAA GCGCCCGGTA GTGATCCTGG 
     3001 GACCCGTGGC CGACATTGCT ATGCAGAAGT TGACTGCTGA GATGCCTGAC CAGTTTGAAA 
     3061 TCGCAGAGAC TGTGTCCAGG ACCGACAGCC CCTCCAAGAT CATCAAACTA GACACCGTGC 
     3121 GGGTGATTGC AGAAAAAGAC AAGCATGCGC TCCTGGATGT GACCCCCTCC GCCATCGAGC 
     3181 GCCTCAACTA TGTGCAGTAC TACCCCATTG TGGTCTTCTT CATCCCCGAG AGCCGGCCGG 
     3241 CCCTCAAGGC ACTGCGCCAG TGGCTGGCGC CTGCCTCCCG CCGCAGCACC CGTCGCCTCT 
     3301 ACGCACAAGC CCAGAAGCTG CGAAAACACA GCAGCCACCT CTTCACAGCC ACCATCCCTC 
     3361 TGAATGGCAC GAGTGACACC TGGTACCAGG AGCTCAAGGC CATCATTCGA GAGCAGCAGA 
     3421 CGCGGCCCAT CTGGACGGCG GAAGATCAGC TGGATGGCTC CTTGGAGGAC AACCTAGACC 
     3481 TCCCTCACCA CGGCCTGGCC GACAGCTCCG CTGACCTCAG CTGCGACAGC CGCGTTAACA 
     3541 GCGACTACGA GACGGACGGC GAGGGCGGCG CGTACACGGA TGGCGAGGGC TACACAGACG 
     3601 GCGAGGGGGG GCCCTACACG GATGTGGATG ATGAGCCCCC GGCTCCAGCC CTGGCCCGGT 
     3661 CCTCGGAGCC CGTGCAGGCA GATGAGTCCC AGAGCCCGAG GGATCGTGGG AGAATCTCGG 
     3721 CTCATCAGGG GGCCCAGGTG GACAGCCGCC ACCCCCAGGG ACAGTGGCGA CAGGACAGCA 
     3781 TGCGAACCTA TGAACGGGAA GCCCTGAAGA AAAAGTTTAT GCGAGTACAT GATGCGGAGT 
     3841 CCTCCGATGA AGACGGCTAT GACTGGGGTC CGGCCACTGA CCTGAGCGGA CCGACGCGTA 
     3901 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     3961 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4021 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4081 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4141 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4201 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4261 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4321 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4381 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4441 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4501 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4561 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4621 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     4681 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     4741 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     4801 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     4861 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     4921 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     4981 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5041 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5101 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5161 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5221 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5281 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5341 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5401 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5461 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     5521 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     5581 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     5641 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     5701 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     5761 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     5821 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     5881 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     5941 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6001 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6061 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6121 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6181 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6241 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6301 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6361 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6421 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6481 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6541 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     6601 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     6661 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     6721 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     6781 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     6841 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     6901 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     6961 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7021 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7081 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7141 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7201 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7261 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7321 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7381 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7441 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7501 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7561 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     7621 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     7681 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     7741 AATTTT
//