LOCUS       RC213258                8703 bp    DNA     circular  SYN 
DEFINITION  MEI1 (Myc-DDK-tagged)-Human meiosis inhibitor 1 (MEI1)
ACCESSION   NM_152513
VERSION     NM_152513.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4860..4951 
                     /label="Myc-DDK"  
     misc_feature    4981..5566 
                     /label="PolyA signal"  
     misc_feature    5038..5057 
                     /label="Restriction Enzyme"  
     misc_feature    5842..6514 
                     /label="ColE1"  
     misc_feature    6944..7738 
                     /label="Kan/Neo"  
     misc_feature    7773..8075 
                     /label="SV40 ori"  
     misc_feature    8161..8638 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4842..4848 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4842 
                     /label="MEI1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGTGAGG CAGGCGGCGA CGGCGGGCAC TCCCGGGCCC AGGAGAGAGG 
     1081 AAGAGGCGGC GCTTCTATTC GAGAGGGCCC ATTACCGGCA CGACCCGCGC TGGCTGCTGC 
     1141 CCGTGACCCC CCGCCTGTGC CTGGCCTGCG CGCTGGAGCT GCTGCCGGAC CCCGGCGTGT 
     1201 CGTTAGTGCG CAAGAAGCAC ATGTTGTCCT GCTTCCAAGA TGCCCTTGTG AGGCATACCT 
     1261 CCCTGGTCAC GCAACTGGTG TCTCAGGATC AGAGAGTCTG CATCCACTTC ATAAGTGTGC 
     1321 TTTTTGGACT ATTATGCAGC ATGGAAGATG GGAGTGTGAC AGACCTCTGT ATTGAAGTCC 
     1381 TTATTCAGAT CACAACGCAG CTGAAGCTGG AGCAGACTAT CCGTTGCCTG CTGGATGAGT 
     1441 GCCACAAAGA GCTGTGTAAC ATGCCCTCCA TGCGAGGCAG CCTGGCCACC CTGACCCTTC 
     1501 TTGGCAAGTT GGTGGATGCC ATCCCTGCTC TGGCAGACGA GCTTGTAATG GAGCATGGCA 
     1561 ACCTGATGGA GCATCTGTTG AGAGGCTTAG TATACCCCAG TGAGGGCATA CAAGCTTCTG 
     1621 TCTGTTACCT TTATGGGAAG CTATACTCCT CACCAGTGGC AGCTGAGATG CTTTCAGGAC 
     1681 ACTTCCGTGA GAAGCTTTTT CCCCTCTTCC TTTCCATCCT GGATGGTGCC CAGACAAAGG 
     1741 AGCTGCAGAT TAACTGCTTG GGTTTGCTGA GGCAGCTGTT GAAGTATGAT CTCTTTGTGT 
     1801 CCATGATCAT GAACCAGGAT GGACTGGGAG AAAGTGCTAA GAATATCGAA GGGTCATCAG 
     1861 GAAATACCTC ACTGCCTTTG GTGCTCAAAA AGCTTCTCCT CTCTAGAGAT GAAACCCTGC 
     1921 AGGTGGCCAG TGCTCACTGT ATAACTGCGG TGCTTGTCCA CTCCCCAGCA AAGCATGCGT 
     1981 CAGCCTTCAT CCACGCTGAC ATCCCAGAGT TCCTCTTTGA GCATCTTTCT TCTTCCAGTG 
     2041 AAGTGCTCGT CTGGTCCAGC TGTAACTGCT TGACACTCCT GGTAGAAGAG CCACTCTTTT 
     2101 TTTCCAAGTG CCACACGGTG TATGGGATCG AGGCAGTGGT GAGGAGCCTG CAGGGAAGCC 
     2161 TGAAGATGAA CAACATAGAG CTGCACAAGC AGGGCCTGCT GCTTTTCGCT GAAATCCTGA 
     2221 CCCGGCAGCC AGAGGAGATC AAGCTGTTCA CAAGCTCAGC CATGTGCAGA GATGCTGGCC 
     2281 GTGCCCTCCA AGAAGCAGTT AGCAGCCCTG TGCTGGAGGT GGCTGCTGAG GCCTTGAAGG 
     2341 CCACTTCTGC TTTTCTGAGG AAGGACCATC AGAGCACTCC ACCTGTGCAG TATGGGGAAC 
     2401 TGCAGGCTTT GCTAGAAGCC ATGCTAAACC GATGTGCGGA GTTTTCCCAG ACCTTGCTGA 
     2461 GCAGGAGGCC CCTGGGCCAT GCCTCCAGTA GAGATTCAGA GAAGGCCATT CTTCAAAGGG 
     2521 GAAAGTTCCT CCTCAGCACT CTGGAGGGAT TTAGAAGTGC CTGCAGGTTG GCTATAGAAT 
     2581 TCCAGAGTGA GCCTTCAGCC CAGGAGAATC CATTCACAGC TCCCAGCGCC AAGAAGGAAG 
     2641 ACACCTTGGA GGCCTTCTCA GAATTTCTTC TCAGTGCCTG TGACTCGCTG TGTATCCCCA 
     2701 TGGTGATGAG ACACTTGGAG CAGACCACCC ACCCAGCTTT GATGGAAGTT TTCCTCTCAA 
     2761 TTCTACATAA CCTCTTTGTC ATCGTTCCCC ACATGAAGGA GAAGTTTTCC AAGAAGCTTG 
     2821 CTTCCTCATC CTTCATACGA CTGACCCTGG AGCTGAAGGC CAGGTTTTGC AGTGGTCTGA 
     2881 GTCACTCAGC CCTAAACCAG GTGTGTTCCA ATTTCCTCTA CTATATGTGC CTCAACCTTC 
     2941 TCTCAGCTCC AGAGAAGACA GGACCACCTT CCAAAGAAGA ACTCTCTGCA GTGTCTGAGC 
     3001 TCCTGCAGCA TGGGCTGCCC CAGATAAGCA GCAGGAGCCC TGAAAGCCTT GCCTTCCTGT 
     3061 CTGATCGCCA GTACATGGAG GGAGCTGCTC GCCAGAGACA GTACTGCATC CTGCTCCTCT 
     3121 TCTACTTGGC CTACATCCAT GAAGACAGGT TTGTCTCAGA GGCAGAGTTA TTTGAGGCTG 
     3181 TGCAAAGCTT CCTCCTGTCG CTGCAGGACC AGGGCGAGCG CCCCCCACTG GTGGTCTTCA 
     3241 AAGCCTCCAT CTATCTGCTT GCAATCTGCC AGGACAAAGA CAATACACTA CGTGAGACTA 
     3301 TGGTCAGTGC AATCAGAAAA TTCCTAGAAG GCATCCCAGA CCTGCAGCTA GTCTATACTC 
     3361 ACCATCCGCT CCTGCTCAGG TTCTTTCTGT TGTATCCAGA GCTCATGAGT AGGTATGGGC 
     3421 ACCGTGTCCT GGAACTTTGG TTCTTCTGGG AAGAGAGCAG CTATGAGGAA CTGGATGATG 
     3481 TCACCTCTGC TGGGCAGCCC GCCCTTCCTG CCAGCTTAGT AGTCCTGTTC CAGTTGCTCA 
     3541 GAAGCATCCC CAGCATCCTG CTCATCTTGC TGGACCTCAT CTATTCCAGC CCAGTGGACA 
     3601 CAGCTCACAA GGTACTGATT AGCCTGAGGA CCTTCCTGAG GAGGAATGAG GATATCCAAG 
     3661 TGGGCGGTCT TATCCGAGGC CACTTCCTGC TGATCCTGCA GCGTCTGCTA GTGGAGCATG 
     3721 GGGCATCCCC ATCAGGAGCC TCGGGGAACC TACCATTGCT GCTGAGCCTC CTCTCCCTGA 
     3781 TGCAGCTCAG GAATGTGTCA GAGCAAGAAC TGGACAGCGT GGCCATGAAG CTCCTTCACC 
     3841 AAGTAAGCAA GCTGTGTGGG AAGTGCAGCC CCACTGACGT GGACATCCTG CAGCCCTCCT 
     3901 TCAACTTCCT GTATTGGAGC CTTCATCAGA CCACACCCAG CAGTCAGAAA AGAGCTGCTG 
     3961 CAGTGCTCCT GAGCAGCACA GGCCTGATGG AGCTTCTGGA GAAGATGCTG GCCCTCACCT 
     4021 TGGCAAAGGC AGATTCTCCC AGGACTGCAC TCCTCTGCTC TGCCTGGCTG CTCACTGCCT 
     4081 CCTTCTCTGC CCAGCAGCAC AAGGGCAGTT TGCAGGTTCA CCAGACACTC TCTGTGGAAA 
     4141 TGGACCAAGT ATTGAAGGCT CTCAGCTTTC CAAAGAAAAA GGCTGCACTA CTCTCAGCTG 
     4201 CCATCTTATG CTTCCTGCGG ACAGCCCTGC GACAAAGCTT TTCCTCTGCC CTGGTAGCCC 
     4261 TGGTGCCCTC AGGGGCCCAG CCACTGCCAG CCACCAAGGA CACTGTCCTA GCTCCACTGC 
     4321 GAATGTCGCA AGTCCGGTCC CTGGTCATTG GGCTGCAGAA CCTCCTGGTG CAGAAGGACC 
     4381 CTCTATTGTC CCAGGCCTGT GTTGGCTGCC TGGAGGCCTT GCTTGACTAC CTGGATGCCC 
     4441 GGAGCCCAGA CATTGCTCTC CACGTGGCCT CCCAGCCTTG GAATCGGTTT TTGCTGTTTA 
     4501 CCCTCTTGGA TGCTGGAGAG AATTCCTTCC TCAGACCTGA GATTTTGAGG CTCATGACCC 
     4561 TGTTTATGCG GTACCGGAGT AGCAGTGTCC TCTCTCATGA AGAGGTGGGT GATGTTCTGC 
     4621 AAGGTGTGGC TTTGGCTGAC CTGTCTACCC TCTCGAACAC CACACTCCAG GCCCTGCATG 
     4681 GCTTCTTCCA GCAGCTCCAG AGCATGGGAC ACCTGGCTGA CCACAGCATG GCCCAGACCC 
     4741 TGCAGGCCTC CTTGGAGGGC CTTCCCCCTA GCACCTCCTC AGGCCAGCCA CCCCTGCAGG 
     4801 ACATGCTCTG CCTGGGAGGG GTGGCTGTAT CCCTGTCCCA CATCAGAAAC ACGCGTACGC 
     4861 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4921 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4981 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5041 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5101 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5161 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5221 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5281 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5341 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5401 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5461 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5521 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5581 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5641 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5701 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5761 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5821 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5881 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5941 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6001 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6061 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6121 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6181 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6241 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6301 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6361 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6421 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6481 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6541 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6601 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6661 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6721 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6781 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6841 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6901 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6961 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7021 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7081 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7141 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7201 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7261 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7321 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7381 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7441 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7501 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7561 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7621 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7681 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7741 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7801 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7861 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7921 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7981 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8041 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8101 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8161 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8221 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8281 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8341 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8401 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8461 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8521 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8581 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8641 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8701 TTT
//