LOCUS       RC213247                8562 bp    DNA     circular  SYN 
DEFINITION  BCR (Myc-DDK-tagged)-Human breakpoint cluster region (BCR), transcript variant 2
ACCESSION   NM_021574
VERSION     NM_021574.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4719..4810 
                     /label="Myc-DDK"  
     misc_feature    4840..5425 
                     /label="PolyA signal"  
     misc_feature    4897..4916 
                     /label="Restriction Enzyme"  
     misc_feature    5701..6373 
                     /label="ColE1"  
     misc_feature    6803..7597 
                     /label="Kan/Neo"  
     misc_feature    7632..7934 
                     /label="SV40 ori"  
     misc_feature    8020..8497 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4701..4707 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4701 
                     /label="BCR"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGGACCCG GTGGGCTTCG CGGAGGCGTG GAAGGCGCAG TTCCCGGACT 
     1081 CAGAGCCCCC GCGCATGGAG CTGCGCTCAG TGGGCGACAT CGAGCAGGAG CTGGAGCGCT 
     1141 GCAAGGCCTC CATTCGGCGC CTGGAGCAGG AGGTGAACCA GGAGCGCTTC CGCATGATCT 
     1201 ACCTGCAGAC GTTGCTGGCC AAGGAAAAGA AGAGCTATGA CCGGCAGCGA TGGGGCTTCC 
     1261 GGCGCGCGGC GCAGGCCCCC GACGGCGCCT CCGAGCCCCG AGCGTCCGCG TCGCGCCCGC 
     1321 AGCCAGCGCC CGCCGACGGA GCCGACCCGC CGCCCGCCGA GGAGCCCGAG GCCCGGCCCG 
     1381 ACGGCGAGGG TTCTCCGGGT AAGGCCAGGC CCGGGACCGC CCGCAGGCCC GGGGCAGCCG 
     1441 CGTCGGGGGA ACGGGACGAC CGGGGACCCC CCGCCAGCGT GGCGGCGCTC AGGTCCAACT 
     1501 TCGAGCGGAT CCGCAAGGGC CATGGCCAGC CCGGGGCGGA CGCCGAGAAG CCCTTCTACG 
     1561 TGAACGTCGA GTTTCACCAC GAGCGCGGCC TGGTGAAGGT CAACGACAAA GAGGTGTCGG 
     1621 ACCGCATCAG CTCCCTGGGC AGCCAGGCCA TGCAGATGGA GCGCAAAAAG TCCCAGCACG 
     1681 GCGCGGGCTC GAGCGTGGGG GATGCATCCA GGCCCCCTTA CCGGGGACGC TCCTCGGAGA 
     1741 GCAGCTGCGG CGTCGACGGC GACTACGAGG ACGCCGAGTT GAACCCCCGC TTCCTGAAGG 
     1801 ACAACCTGAT CGACGCCAAT GGCGGTAGCA GGCCCCCTTG GCCGCCCCTG GAGTACCAGC 
     1861 CCTACCAGAG CATCTACGTC GGGGGCATGA TGGAAGGGGA GGGCAAGGGC CCGCTCCTGC 
     1921 GCAGCCAGAG CACCTCTGAG CAGGAGAAGC GCCTTACCTG GCCCCGCAGG TCCTACTCCC 
     1981 CCCGGAGTTT TGAGGATTGC GGAGGCGGCT ATACCCCGGA CTGCAGCTCC AATGAGAACC 
     2041 TCACCTCCAG CGAGGAGGAC TTCTCCTCTG GCCAGTCCAG CCGCGTGTCC CCAAGCCCCA 
     2101 CCACCTACCG CATGTTCCGG GACAAAAGCC GCTCTCCCTC GCAGAACTCG CAACAGTCCT 
     2161 TCGACAGCAG CAGTCCCCCC ACGCCGCAGT GCCATAAGCG GCACCGGCAC TGCCCGGTTG 
     2221 TCGTGTCCGA GGCCACCATC GTGGGCGTCC GCAAGACCGG GCAGATCTGG CCCAACGATG 
     2281 GCGAGGGCGC CTTCCATGGA GACGCAGATG GCTCGTTCGG AACACCACCT GGATACGGCT 
     2341 GCGCTGCAGA CCGGGCAGAG GAGCAGCGCC GGCACCAAGA TGGGCTGCCC TACATTGATG 
     2401 ACTCGCCCTC CTCATCGCCC CACCTCAGCA GCAAGGGCAG GGGCAGCCGG GATGCGCTGG 
     2461 TCTCGGGAGC CCTGGAGTCC ACTAAAGCGA GTGAGCTGGA CTTGGAAAAG GGCTTGGAGA 
     2521 TGAGAAAATG GGTCCTGTCG GGAATCCTGG CTAGCGAGGA GACTTACCTG AGCCACCTGG 
     2581 AGGCACTGCT GCTGCCCATG AAGCCTTTGA AAGCCGCTGC CACCACCTCT CAGCCGGTGC 
     2641 TGACGAGTCA GCAGATCGAG ACCATCTTCT TCAAAGTGCC TGAGCTCTAC GAGATCCACA 
     2701 AGGAGTTCTA TGATGGGCTC TTCCCCCGCG TGCAGCAGTG GAGCCACCAG CAGCGGGTGG 
     2761 GCGACCTCTT CCAGAAGCTG GCCAGCCAGC TGGGTGTGTA CCGGGCCTTC GTGGACAACT 
     2821 ACGGAGTTGC CATGGAAATG GCTGAGAAGT GCTGTCAGGC CAATGCTCAG TTTGCAGAAA 
     2881 TCTCCGAGAA CCTGAGAGCC AGAAGCAACA AAGATGCCAA GGATCCAACG ACCAAGAACT 
     2941 CTCTGGAAAC TCTGCTCTAC AAGCCTGTGG ACCGTGTGAC GAGGAGCACG CTGGTCCTCC 
     3001 ATGACTTGCT GAAGCACACT CCTGCCAGCC ACCCTGACCA CCCCTTGCTG CAGGACGCCC 
     3061 TCCGCATCTC ACAGAACTTC CTGTCCAGCA TCAATGAGGA GATCACACCC CGACGGCAGT 
     3121 CCATGACGGT GAAGAAGGGA GAGCACCGGC AGCTGCTGAA GGACAGCTTC ATGGTGGAGC 
     3181 TGGTGGAGGG GGCCCGCAAG CTGCGCCACG TCTTCCTGTT CACCGACCTG CTTCTCTGCA 
     3241 CCAAGCTCAA GAAGCAGAGC GGAGGCAAAA CGCAGCAGTA TGACTGCAAA TGGTACATTC 
     3301 CGCTCACGGA TCTCAGCTTC CAGATGGTGG ATGAACTGGA GGCAGTGCCC AACATCCCCC 
     3361 TGGTGCCCGA TGAGGAGCTG GACGCTTTGA AGATCAAGAT CTCCCAGATC AAGAATGACA 
     3421 TCCAGAGAGA GAAGAGGGCG AACAAGGGCA GCAAGGCTAC GGAGAGGCTG AAGAAGAAGC 
     3481 TGTCGGAGCA GGAGTCACTG CTGCTGCTTA TGTCTCCCAG CATGGCCTTC AGGGTGCACA 
     3541 GCCGCAACGG CAAGAGTTAC ACGTTCCTGA TCTCCTCTGA CTATGAGCGT GCAGAGTGGA 
     3601 GGGAGAACAT CCGGGAGCAG CAGAAGAAGT GTTTCAGAAG CTTCTCCCTG ACATCCGTGG 
     3661 AGCTGCAGAT GCTGACCAAC TCGTGTGTGA AACTCCAGAC TGTCCACAGC ATTCCGCTGA 
     3721 CCATCAATAA GGAAGATGAT GAGTCTCCGG GGCTCTATGG GTTTCTGAAT GTCATCGTCC 
     3781 ACTCAGCCAC TGGATTTAAG CAGAGTTCAA ATCTGTACTG CACCCTGGAG GTGGATTCCT 
     3841 TTGGGTATTT TGTGAATAAA GCAAAGACGC GCGTCTACAG GGACACAGCT GAGCCAAACT 
     3901 GGAACGAGCT GGACCCGCAG GCCCTGCAGG ACAGAGACTG GCAGCGCACC GTCATCGCCA 
     3961 TGAATGGGAT CGAAGTAAAG CTCTCGGTCA AGTTCAACAG CAGGGAGTTC AGCTTGAAGA 
     4021 GGATGCCGTC CCGAAAACAG ACAGGGGTCT TCGGAGTCAA GATTGCTGTG GTCACCAAGA 
     4081 GAGAGAGGTC CAAGGTGCCC TACATCGTGC GCCAGTGCGT GGAGGAGATC GAGCGCCGAG 
     4141 GCATGGAGGA GGTGGGCATC TACCGCGTGT CCGGTGTGGC CACGGACATC CAGGCACTGA 
     4201 AGGCAGCCTT CGACGTCAAT AACAAGGACG TGTCGGTGAT GATGAGCGAG ATGGACGTGA 
     4261 ACGCCATCGC AGGCACGCTG AAGCTGTACT TCCGTGAGCT GCCCGAGCCC CTCTTCACTG 
     4321 ACGAGTTCTA CCCCAACTTC GCAGAGGGCA TCGCTCTTTC AGACCCGGTT GCAAAGGAGA 
     4381 GCTGCATGCT CAACCTGCTG CTGTCCCTGC CGGAGGCCAA CCTGCTCACC TTCCTTTTCC 
     4441 TTCTGGACCA CCTGAAAAGG GTGGCAGAGA AGGAGGCAGT CAATAAGATG TCCCTGCACA 
     4501 ACCTCGCCAC GGTCTTTGGC CCCACGCTGC TCCGGCCCTC CGAGAAGGAG AGCAAGCTCC 
     4561 CTGCCAACCC CAGCCAGCCT ATCACCATGA CTGACAGCTG GTCCTTGGAG GTCATGTCCC 
     4621 AGGTCCAGGT GCTGCTGTAC TTCCTGCAGC TGGAGGCCAT CCCTGCCCCG GACAGCAAGA 
     4681 GACAGAGCAT CCTGTTCTCC ACCGAAGTCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4741 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4801 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4861 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4921 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4981 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5041 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5101 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5161 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5221 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5281 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5341 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5401 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5461 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5521 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5581 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5641 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5701 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5761 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5821 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5881 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5941 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6001 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6061 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6121 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6181 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6241 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6301 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6361 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6421 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6481 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6541 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6601 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6661 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6721 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6781 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6841 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6901 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6961 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7021 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7081 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7141 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7201 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7261 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7321 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7381 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7441 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7501 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7561 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7621 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7681 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7741 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7801 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7861 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7921 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7981 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8041 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8101 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8161 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8221 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8281 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8341 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8401 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8461 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8521 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//